(Circular) (Six-base) MAPSORT of: pFN21AB6929.seq Check: 4849 from: 1 to: 5326 pFN21AB6929 5326 bp With 182 enzymes: SgfI * October 20, 2009 10:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2373 2373 Size: 5326 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3136 278 Size: 53 83 186 936 1600 2468 Fragments arranged by size: 2468 1600 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2669 1937 Size: 732 4594 Acc65I G'GTAC_C Cuts at: 2497 2647 2497 Size: 150 5176 AclI AA'CG_TT Cuts at: 1563 3453 3826 1563 Size: 1890 373 3063 Fragments arranged by size: 3063 1890 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3389 4773 5041 1632 Size: 1757 1384 268 1917 Fragments arranged by size: 1917 1757 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5129 AflIII A'CryG_T Cuts at: 1203 4225 5202 1203 Size: 3022 977 1327 Fragments arranged by size: 3022 1327 977 AgeI A'CCGG_T Cuts at: 4157 4157 Size: 5326 AhdI GACnn_n'nnGTC Cuts at: 2232 4055 2232 Size: 1823 3503 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5326 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5326 AlwNI CAG_nnn'CTG Cuts at: 2622 3069 4641 2622 Size: 447 1572 3307 Fragments arranged by size: 3307 1572 447 ApaI G_GGCC'C Cuts at: 2414 2537 2414 Size: 123 5203 ApaLI G'TGCA_C Cuts at: 2381 3383 4539 2381 Size: 1002 1156 3168 Fragments arranged by size: 3168 1156 1002 ApoI r'AATT_y Cuts at: 1493 2635 2861 3013 5122 1493 Size: 1142 226 152 2109 1697 Fragments arranged by size: 2109 1697 1142 226 152 AseI AT'TA_AT Cuts at: 160 1032 3880 160 Size: 872 2848 1606 Fragments arranged by size: 2848 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5326 AvaI C'yCGr_G Cuts at: 1870 1943 2066 2074 2651 1870 Size: 73 123 8 577 4545 Fragments arranged by size: 4545 577 123 73 8 BamHI G'GATC_C Cuts at: 2061 2656 3023 2061 Size: 595 367 4364 Fragments arranged by size: 4364 595 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2088 2497 2647 4102 618 Size: 325 217 627 301 409 150 1455 1842 Fragments arranged by size: 1842 1455 627 409 325 301 217 150 BanII G_rGCy'C Cuts at: 1391 2414 2537 2558 2577 2645 1391 Size: 1023 123 21 19 68 4072 Fragments arranged by size: 4072 1023 123 68 21 19 BbeI G_GCGC'C Cuts at: 2092 2092 Size: 5326 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4355 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3517 3551 1903 Size: 34 1580 34 3678 Fragments arranged by size: 3678 1580 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3218 4428 3218 Size: 1210 4116 BclI T'GATC_A Cuts at: 1548 1548 Size: 5326 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3937 136 Size: 107 122 71 631 605 2265 1525 Fragments arranged by size: 2265 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5321 5321 Size: 5326 BlpI GC'TnA_GC Cuts at: 2743 2743 Size: 5326 Bme1580I G_kGCm'C Cuts at: 1790 2385 2414 2537 3387 4543 1790 Size: 595 29 123 850 1156 2573 Fragments arranged by size: 2573 1156 850 595 123 29 BmgBI CAC'GTC Cuts at: 1318 2048 1318 Size: 730 4596 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3057 4015 442 Size: 416 53 83 399 24 1640 958 1753 Fragments arranged by size: 1753 1640 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5326 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3986 1239 Size: 2747 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3451 4316 4614 3451 Size: 865 298 4163 Fragments arranged by size: 4163 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3989 882 Size: 617 2490 2219 Fragments arranged by size: 2490 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3117 493 Size: 711 1913 2702 Fragments arranged by size: 2702 1913 711 BsaBI GATnn'nnATC Cuts at: 5320 5320 Size: 5326 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2089 2187 3133 3515 Size: 53 83 186 936 556 98 946 382 Cuts at: 3515 4150 275 Size: 635 1451 Fragments arranged by size: 1451 946 936 635 556 382 186 98 83 53 BsaWI w'CCGG_w Cuts at: 3759 4157 4431 4578 4989 3759 Size: 398 274 147 411 4096 Fragments arranged by size: 4096 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5101 Fragments arranged by size: 5101 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2276 1186 Size: 1090 4236 BseYI C'CCAG_C Cuts at: 4529 5025 4529 Size: 496 4830 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2321 2420 1163 Size: 573 585 99 4069 Fragments arranged by size: 4069 585 573 99 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2460 2776 3537 4565 1540 Size: 275 186 459 316 761 1028 2301 Fragments arranged by size: 2301 1028 761 459 316 275 186 BsiHKAI G_wGCw'C Cuts at: 2021 2385 2577 3387 3472 4543 2021 Size: 364 192 810 85 1071 2804 Fragments arranged by size: 2804 1071 810 364 192 85 BsmI GAATG_Cn' Cuts at: 2837 2930 2837 Size: 93 5233 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2021 2385 2414 2537 2558 2577 2645 Size: 399 231 364 29 123 21 19 68 Cuts at: 2645 3387 3472 4543 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 364 231 123 85 68 29 21 19 BspEI T'CCGG_A Cuts at: 4989 4989 Size: 5326 BspHI T'CATG_A Cuts at: 3215 4945 3215 Size: 1730 3596 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2373 2442 2681 844 Size: 1529 69 239 3489 Fragments arranged by size: 3489 1529 239 69 BsrBI CCG'CTC Cuts at: 1394 1823 3213 1394 Size: 429 1390 3507 Fragments arranged by size: 3507 1390 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3821 3995 65 Size: 1183 2573 174 1396 Fragments arranged by size: 2573 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2455 3970 4157 1700 Size: 110 645 1515 187 2869 Fragments arranged by size: 2869 1515 645 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5326 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5326 BssSI C'ACGA_G Cuts at: 3386 4398 3386 Size: 1012 4314 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5326 BstBI TT'CG_AA Cuts at: 4130 4130 Size: 5326 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5326 BstXI CCAn_nnnn'nTGG Cuts at: 2551 2551 Size: 5326 BstYI r'GATC_y Cuts at: 1972 2061 2211 2656 3023 3408 3425 4877 4963 Size: 89 150 445 367 385 17 1452 86 Cuts at: 4963 4975 5321 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 445 385 367 346 150 89 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2037 2037 Size: 5326 BtgI C'CryG_G Cuts at: 513 1065 2529 2550 513 Size: 552 1464 21 3289 Fragments arranged by size: 3289 1464 552 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4587 Fragments arranged by size: 4587 683 56 BtsI GCAGTG_nn' Cuts at: 2578 2842 3636 3656 2578 Size: 264 794 20 4248 Fragments arranged by size: 4248 794 264 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5326 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5291 DraI TTT'AAA Cuts at: 2630 2630 Size: 5326 DraIII CAC_nnn'GTG Cuts at: 2530 2530 Size: 5326 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4333 809 Size: 507 3017 1802 Fragments arranged by size: 3017 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2376 2457 2773 Size: 54 1425 50 275 18 546 81 316 Cuts at: 2773 3662 5291 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 546 316 275 81 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2457 2773 1537 Size: 275 645 316 4090 Fragments arranged by size: 4090 645 316 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3256 1353 Size: 1903 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3913 4287 4433 387 Size: 1064 138 2324 374 146 1280 Fragments arranged by size: 2324 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2575 2575 Size: 5326 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3389 3986 4773 5041 1239 Size: 393 1757 597 787 268 1524 Fragments arranged by size: 1757 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2041 2410 2411 2534 2041 Size: 369 1 123 4833 Fragments arranged by size: 4833 369 123 1 EcoRI G'AATT_C Cuts at: 2635 3013 2635 Size: 378 4948 FspI TGC'GCA Cuts at: 2609 3832 2609 Size: 1223 4103 HaeII r_GCGC'y Cuts at: 1678 2092 2148 2431 4473 1678 Size: 414 56 283 2042 2531 Fragments arranged by size: 2531 2042 414 283 56 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2218 2250 3967 3999 4041 Size: 32 135 32 646 32 1717 32 42 Cuts at: 4041 4073 1373 Size: 32 2626 Fragments arranged by size: 2626 1717 646 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2670 2916 669 Size: 988 281 732 246 3079 Fragments arranged by size: 3079 988 732 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5326 HpaI GTT'AAC Cuts at: 2916 2916 Size: 5326 KasI G'GCGC_C Cuts at: 2088 2088 Size: 5326 KpnI G_GTAC'C Cuts at: 2501 2651 2501 Size: 150 5176 MfeI C'AATT_G Cuts at: 2925 2925 Size: 5326 MluI A'CGCG_T Cuts at: 5202 5202 Size: 5326 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4440 4624 1305 Size: 3135 184 2007 Fragments arranged by size: 3135 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3504 Fragments arranged by size: 3504 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2367 2549 2567 3284 3643 3802 518 Size: 648 1201 182 18 717 359 159 2042 Fragments arranged by size: 2042 1201 717 648 359 182 159 18 MspA1I CmG'CkG Cuts at: 1606 2086 2619 2742 3419 4567 4812 1606 Size: 480 533 123 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 533 480 245 123 NaeI GCC'GGC Cuts at: 2457 2457 Size: 5326 NarI GG'CG_CC Cuts at: 2089 2089 Size: 5326 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4774 NdeI CA'TA_TG Cuts at: 387 387 Size: 5326 NgoMIV G'CCGG_C Cuts at: 2455 2455 Size: 5326 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5326 NotI GC'GGCC_GC Cuts at: 2773 2773 Size: 5326 NspI r_CATG'y Cuts at: 1132 1229 2372 2684 4229 1132 Size: 97 1143 312 1545 2229 Fragments arranged by size: 2229 1545 1143 312 97 PasI CC'CwG_GG Cuts at: 1348 2248 1348 Size: 900 4426 PciI A'CATG_T Cuts at: 4225 4225 Size: 5326 PflMI CCAn_nnn'nTGG Cuts at: 1108 2530 1108 Size: 1422 3904 PmeI GTTT'AAAC Cuts at: 2630 2630 Size: 5326 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3395 3427 4908 4940 3395 Size: 32 1481 32 3781 Fragments arranged by size: 3781 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5326 PsiI TTA'TAA Cuts at: 2896 2896 Size: 5326 PspOMI G'GGCC_C Cuts at: 2410 2533 2410 Size: 123 5203 PstI C_TGCA'G Cuts at: 830 2678 830 Size: 1848 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5326 SacI G_AGCT'C Cuts at: 2577 2577 Size: 5326 SalI G'TCGA_C Cuts at: 1936 2668 1936 Size: 732 4594 SbfI CC_TGCA'GG Cuts at: 2678 2678 Size: 5326 ScaI AGT'ACT Cuts at: 3574 5286 3574 Size: 1712 3614 SfcI C'TryA_G Cuts at: 826 1045 2674 3809 4490 4681 826 Size: 219 1629 1135 681 191 1471 Fragments arranged by size: 1629 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2090 2090 Size: 5326 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5326 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5326 SmaI CCC'GGG Cuts at: 1872 2653 1872 Size: 781 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3430 4331 4593 820 Size: 197 926 1487 901 262 1553 Fragments arranged by size: 1553 1487 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3117 493 Size: 2624 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5326 SphI G_CATG'C Cuts at: 1132 2684 1132 Size: 1552 3774 SspI AAT'ATT Cuts at: 5 52 3250 5 Size: 47 3198 2081 Fragments arranged by size: 3198 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5326 StyI C'CwwG_G Cuts at: 513 1065 2765 513 Size: 552 1700 3074 Fragments arranged by size: 3074 1700 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2036 3353 3370 3523 3708 4153 325 Size: 1255 456 1317 17 153 185 445 1498 Fragments arranged by size: 1498 1317 1255 456 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3572 5284 96 Size: 274 80 33 51 1443 1595 1712 138 Fragments arranged by size: 1712 1595 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2046 2078 5286 5318 2046 Size: 32 3208 32 2054 Fragments arranged by size: 3208 2054 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2138 1316 Size: 822 4504 XbaI T'CTAG_A Cuts at: 2662 2662 Size: 5326 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5326 XmaI C'CCGG_G Cuts at: 1870 2651 1870 Size: 781 4545 XmnI GAAnn'nnTTC Cuts at: 3455 3455 Size: 5326 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3134 276 Size: 53 83 186 936 1600 2468 Fragments arranged by size: 2468 1600 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI