Restriction map for
pFN21AB6926
##################################
# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcsI AflII AgeI AhdI Alw44I
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AvaII AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BfrI BlnI BlpI
Bpu1102I BsaAI BsaBI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspMI BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I CelII ClaI CpoI DraI
DraIII DsaI EagI Eam1105I EarI
EclXI Eco52I EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MamI MfeI MluI MseI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccIII 181 TCCGGA T^CCGGA
AcyI 130 GGCGTC G[GA]^CG[CT]C
AfaI 507 GTAC GT^AC
AflIII 142 ACATGT A^C[GA][CT]GT
AocI 92 CCTCAGG CC^T[ACGT]AGG
Aor51HI 659 AGCGCT AGC^GCT
BanII 616 GAGCCC G[GA]GC[CT]^C
BbsI 481 GAAGAC GAAGAC(2/6)
BclI 425 TGATCA T^GATCA
BfaI 436 CTAG C^TAG
BglI 562 GCCTTTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 260 AGATCT A^GATCT
BpuAI 481 GAAGAC GAAGAC(2/6)
BsaHI 130 GGCGTC G[GA]^CG[CT]C
BsaWI 181 TCCGGA [AT]^CCGG[AT]
BseAI 181 TCCGGA T^CCGGA
BsiYI 494 CCTTGGCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 494 CCTTGGCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspEI 181 TCCGGA T^CCGGA
BspHI 399 TCATGA T^CATGA
BspLU11I 142 ACATGT A^CATGT
BsrFI 33 GCCGGT [GA]^CCGG[CT]
BsrI 541 ACTGG ACTGG(1/-1)
BstAPI 69 GCAGACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 92 CCTCAGG CC^T[ACGT]AGG
Cfr10I 33 GCCGGT [GA]^CCGG[CT]
DrdI 124 GACACAGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 659 AGCGCT AGC^GCT
Eco81I 92 CCTCAGG CC^T[ACGT]AGG
EcoNI 494 CCTTGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 56 CCCGC CCCGC(4/6)
FbaI 425 TGATCA T^GATCA
Hin1I 130 GGCGTC G[GA]^CG[CT]C
MaeI 436 CTAG C^TAG
MaeII 419 ACGT A^CGT
MroI 181 TCCGGA T^CCGGA
NspI 142 ACATGT [GA]CATG^[CT]
PleI 646 GAGTC GAGTC(4/5)
PstI 256 CTGCAG CTGCA^G
PvuII 666 CAGCTG CAG^CTG
RcaI 399 TCATGA T^CATGA
RsaI 507 GTAC GT^AC
SfcI 256 CTGCAG C^T[GA][CT]AG
StuI 326 AGGCCT AGG^CCT
TaqI 368 TCGA T^CGA
TfiI 396 GATTC G^A[AT]TC
Tsp509I 158 AATT ^AATT
TspRI 390 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 368 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
BalI 497 TGGCCA TGG^CCA
569 TGGCCA
BcnI 594 CCGGG CC^[GC]GG
599 CCGGG
BmyI 88 GTGCCC G[AGT]GC[ACT]^C
616 GAGCCC
BpmI 663 CTCCAG CTGGAG(16/14)
523 CTGGAG
BsaI 490 GAGACC GGTCTC(1/5)
526 GAGACC
Bsp1286I 88 GTGCCC G[AGT]GC[ACT]^C
616 GAGCCC
BstF5I 237 GGATG GGATG(2/0)
612 GGATG
BstYI 260 AGATCT [GA]^GATC[CT]
629 AGATCC
Cac8I 55 GCCCGC GC[ACGT]^[ACGT]GC
86 GCGTGC
DraII 169 GGGGCCT [GA]G^G[ACGT]CC[CT]
379 AGGGCCT
EaeI 497 TGGCCA [CT]^GGCC[GA]
569 TGGCCA
EcoO109I 169 GGGGCCT [GA]G^G[ACGT]CC[CT]
379 AGGGCCT
EcoT14I 494 CCTTGG C^C[AT][AT]GG
578 CCAAGG
FokI 237 GGATG GGATG(9/13)
612 GGATG
HaeII 532 AGCGCC [GA]GCGC^[CT]
659 AGCGCT
HgaI 131 GCGTC GACGC(5/10)
296 GCGTC
HinfI 396 GATTC G^A[ACGT]TC
646 GAGTC
MaeIII 229 GTGAC ^GT[ACGT]AC
298 GTCAC
MflI 260 AGATCT [GA]^GATC[CT]
629 AGATCC
MluNI 497 TGGCCA TGG^CCA
569 TGGCCA
MscI 497 TGGCCA TGG^CCA
569 TGGCCA
MslI 400 CATGAGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
546 CATGAATGTG
MspA1I 272 CCGCTG C[AC]G^C[GT]G
666 CAGCTG
NciI 594 CCGGG CC^[GC]GG
599 CCGGG
StyI 494 CCTTGG C^C[AT][AT]GG
578 CCAAGG
Tsp45I 229 GTGAC ^GT[GC]AC
298 GTCAC
XhoII 260 AGATCT [GA]^GATC[CT]
629 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AluI AlwI AlwNI BbvI
BsaJI BsmAI BsmFI BssKI BstNI
CfoI Cfr13I DdeI DpnII EcoRII
Fnu4HI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MboI MboII
MnlI MspI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI TseI