Restriction map for
pFN21AB6926
(Linear) (Six-base) MAPSORT of: KIBB6926.seq Check: 3580 from: 1 to: 684
KIBB6926.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 198 684
Size: 198 486
AfeI AGC'GCT
Cuts at: 0 661 684
Size: 661 23
AflIII A'CryG_T
Cuts at: 0 142 684
Size: 142 542
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 127 684
Size: 127 557
AlwNI CAG_nnn'CTG
Cuts at: 0 75 383 671 684
Size: 75 308 288 13
Fragments arranged by size:
308 288 75 13
BanII G_rGCy'C
Cuts at: 0 620 684
Size: 620 64
BbsI GAAGACnn'nnnn_
Cuts at: 0 488 684
Size: 488 196
BclI T'GATC_A
Cuts at: 0 425 684
Size: 425 259
BglI GCCn_nnn'nGGC
Cuts at: 0 568 684
Size: 568 116
BglII A'GATC_T
Cuts at: 0 260 684
Size: 260 424
Bme1580I G_kGCm'C
Cuts at: 0 92 684
Size: 92 592
BmgBI CAC'GTC
Cuts at: 0 420 684
Size: 420 264
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 544 648 684
Size: 544 104 36
Fragments arranged by size:
544 104 36
Bpu10I CC'TnA_GC
Cuts at: 0 43 323 684
Size: 43 280 361
Fragments arranged by size:
361 280 43
BsaI GGTCTCn'nnnn_
Cuts at: 0 484 520 684
Size: 484 36 164
Fragments arranged by size:
484 164 36
BsaHI Gr'CG_yC
Cuts at: 0 131 684
Size: 131 553
BsaWI w'CCGG_w
Cuts at: 0 181 684
Size: 181 503
BseYI C'CCAG_C
Cuts at: 0 274 619 684
Size: 274 345 65
Fragments arranged by size:
345 274 65
Bsp1286I G_dGCh'C
Cuts at: 0 92 620 684
Size: 92 528 64
Fragments arranged by size:
528 92 64
BspEI T'CCGG_A
Cuts at: 0 181 684
Size: 181 503
BspHI T'CATG_A
Cuts at: 0 399 684
Size: 399 285
BsrFI r'CCGG_y
Cuts at: 0 33 684
Size: 33 651
BstAPI GCAn_nnn'nTGC
Cuts at: 0 75 684
Size: 75 609
BstYI r'GATC_y
Cuts at: 0 260 629 684
Size: 260 369 55
Fragments arranged by size:
369 260 55
Bsu36I CC'TnA_GG
Cuts at: 0 93 684
Size: 93 591
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 558 684
Size: 558 126
DrdI GACnn_nn'nnGTC
Cuts at: 0 130 684
Size: 130 554
EaeI y'GGCC_r
Cuts at: 0 497 569 684
Size: 497 72 115
Fragments arranged by size:
497 115 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 463 625 684
Size: 463 162 59
Fragments arranged by size:
463 162 59
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 198 544 648 684
Size: 198 346 104 36
Fragments arranged by size:
346 198 104 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 498 684
Size: 498 186
EcoO109I rG'GnC_Cy
Cuts at: 0 170 380 684
Size: 170 210 304
Fragments arranged by size:
304 210 170
HaeII r_GCGC'y
Cuts at: 0 536 663 684
Size: 536 127 21
Fragments arranged by size:
536 127 21
MscI TGG'CCA
Cuts at: 0 499 571 684
Size: 499 72 113
Fragments arranged by size:
499 113 72
MslI CAynn'nnrTG
Cuts at: 0 404 550 684
Size: 404 146 134
Fragments arranged by size:
404 146 134
MspA1I CmG'CkG
Cuts at: 0 274 668 684
Size: 274 394 16
Fragments arranged by size:
394 274 16
NspI r_CATG'y
Cuts at: 0 146 684
Size: 146 538
PciI A'CATG_T
Cuts at: 0 142 684
Size: 142 542
PfoI T'CCnGG_A
Cuts at: 0 521 684
Size: 521 163
PstI C_TGCA'G
Cuts at: 0 260 684
Size: 260 424
PvuII CAG'CTG
Cuts at: 0 668 684
Size: 668 16
SfcI C'TryA_G
Cuts at: 0 256 684
Size: 256 428
StuI AGG'CCT
Cuts at: 0 328 684
Size: 328 356
StyI C'CwwG_G
Cuts at: 0 494 578 684
Size: 494 84 106
Fragments arranged by size:
494 106 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 316 684
Size: 316 368
Enzymes that do cut:
AcuI AfeI AflIII AlfI AlwNI BanII BbsI BclI
BglI BglII Bme1580I BmgBI BpmI Bpu10I BsaI BsaHI
BsaWI BseYI Bsp1286I BspEI BspHI BsrFI BstAPI BstYI
Bsu36I BtgZI DrdI EaeI EciI Eco57MI EcoNI EcoO109I
HaeII MscI MslI MspA1I NspI PciI PfoI PstI
PvuII SfcI StuI StyI TaqII
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AleI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BciVI BfrBI BlpI BmrI BmtI BplI BpuEI BsaAI
BsaBI BsaXI BseRI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspMI BsrBI BsrDI BsrGI BssHII BssSI BstBI
BstEII BstXI BstZ17I BtgI BtsI ClaI CspCI DraI
DraIII EagI EarI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI