Restriction map for
pFN21AB6925
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# NO CUT ENZYMES ( 155 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI Asp700I Asp718I
AspEI AspHI AspI AvaI AvaII
AviII AvrII BalI BamHI BanII
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfrI BglII BlnI
BmyI BpmI BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BsgI BsiHKAI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I Cac8I ClaI CpoI DraI
DraII DraIII DrdI Eam1105I EarI
Eco47III Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HpaI KpnI Ksp632I MamI
MfeI MluI MluNI MroI MscI
MunI NaeI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI RcaI RsaI
RsrII SacI SapI SbfI ScaI
SexAI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccI 106 GTCGAC GT^[AC][GT]AC
AccII 97 CGCG CG^CG
AseI 477 ATTAAT AT^TAAT
AsnI 477 ATTAAT AT^TAAT
BanI 285 GGCGCC G^G[CT][GA]CC
BbeI 285 GGCGCC GGCGC^C
BfaI 466 CTAG C^TAG
BglI 7 GCCTCGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 117 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 117 GCTGAGC GC^T[ACGT]AGC
BsiEI 83 CGGCCG CG[GA][CT]^CG
BsmAI 526 GAGAC GTCTC(1/5)
BsrFI 23 ACCGGC [GA]^CCGG[CT]
BssKI 302 CCAGG ^CC[ACGT]GG
BstNI 302 CCAGG CC^[AT]GG
BstUI 97 CGCG CG^CG
CelII 117 GCTGAGC GC^T[ACGT]AGC
CfoI 286 GCGC GCG^C
Cfr10I 23 ACCGGC [GA]^CCGG[CT]
Cfr13I 26 GGCCC G^G[ACGT]CC
EaeI 83 CGGCCG [CT]^GGCC[GA]
EagI 83 CGGCCG C^GGCCG
EclXI 83 CGGCCG C^GGCCG
Eco52I 83 CGGCCG C^GGCCG
EcoRII 302 CCAGG ^CC[AT]GG
EcoT14I 281 CCATGG C^C[AT][AT]GG
HaeII 285 GGCGCC [GA]GCGC^[CT]
HapII 24 CCGG C^CGG
HgaI 79 GCGTC GACGC(5/10)
HhaI 286 GCGC GCG^C
HinP1I 286 GCGC G^CGC
HincII 106 GTCGAC GT[CT]^[GA]AC
HindII 106 GTCGAC GT[CT]^[GA]AC
HindIII 433 AAGCTT A^AGCTT
HpaII 24 CCGG C^CGG
HphI 388 TCACC GGTGA(8/7)
KasI 285 GGCGCC G^GCGCC
KspI 96 CCGCGG CCGC^GG
MaeI 466 CTAG C^TAG
MaeII 163 ACGT A^CGT
MspI 24 CCGG C^CGG
MvaI 302 CCAGG CC^[AT]GG
MvnI 97 CGCG CG^CG
NarI 285 GGCGCC GG^CGCC
NcoI 281 CCATGG C^CATGG
PleI 452 GACTC GAGTC(4/5)
PshBI 477 ATTAAT AT^TAAT
PvuII 317 CAGCTG CAG^CTG
SacII 96 CCGCGG CCGC^GG
SalI 106 GTCGAC G^TCGAC
Sau96I 26 GGCCC G^G[ACGT]CC
ScrFI 302 CCAGG CC^[ACGT]GG
SfaNI 250 GATGC GCATC(5/9)
SfiI 6 GGCCTCGACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 285 GGCGCC GGC^GCC
StyI 281 CCATGG C^C[AT][AT]GG
XcmI 272 CCACTGAAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 78 GGCGTC G[GA]^CG[CT]C
285 GGCGCC
AlwI 38 GGATC GGATC(4/5)
571 GGATC
BbvI 316 GCAGC GCAGC(8/12)
313 GCTGC
BsaHI 78 GGCGTC G[GA]^CG[CT]C
285 GGCGCC
BseRI 112 GAGGAG GAGGAG(10/8)
370 GAGGAG
BstYI 38 GGATCT [GA]^GATC[CT]
571 GGATCT
DdeI 118 CTGAG C^T[ACGT]AG
297 CTTAG
DpnII 39 GATC ^GATC
572 GATC
DsaI 96 CCGCGG C^C[GA][CT]GG
281 CCATGG
FauI 95 CCCGC CCCGC(4/6)
98 GCGGG
Hin1I 78 GGCGTC G[GA]^CG[CT]C
285 GGCGCC
MboI 39 GATC ^GATC
572 GATC
MflI 38 GGATCT [GA]^GATC[CT]
571 GGATCT
MseI 193 TTAA T^TAA
478 TTAA
MslI 439 CACTACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
496 CATGATGATG
MspA1I 96 CCGCGG C[AC]G^C[GT]G
317 CAGCTG
MwoI 7 GCCTCGACGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
271 GCCACTGAAGC
NdeII 39 GATC ^GATC
572 GATC
NlaIV 285 GGCGCC GG[ACGT]^[ACGT]CC
330 GGAGCC
PstI 314 CTGCAG CTGCA^G
530 CTGCAG
Sau3AI 39 GATC ^GATC
572 GATC
TaqI 10 TCGA T^CGA
107 TCGA
TfiI 345 GATTC G^A[AT]TC
559 GAATC
Tru9I 193 TTAA T^TAA
478 TTAA
TseI 313 GCTGC G^C[AT]GC
316 GCAGC
TspRI 273 CACTG CA[GC]TG[ACGT][ACGT]^
363 CAGTG
TthHB8I 10 TCGA T^CGA
107 TCGA
XhoII 38 GGATCT [GA]^GATC[CT]
571 GGATCT
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# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AciI AluI BsaJI BsiYI BslI
BstF5I Fnu4HI FokI HaeIII HinfI
ItaI MaeIII MboII MnlI NlaIII
SfcI Tsp45I Tsp509I