Restriction map for
pFN21AB6925
(Linear) (Six-base) MAPSORT of: KIBB6925.seq Check: 6504 from: 1 to: 615
KIBB6925.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AccI GT'mk_AC
Cuts at: 0 107 615
Size: 107 508
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 341 413 615
Size: 296 45 72 202
Fragments arranged by size:
296 202 72 45
AseI AT'TA_AT
Cuts at: 0 478 615
Size: 478 137
BanI G'GyrC_C
Cuts at: 0 285 615
Size: 285 330
BbeI G_GCGC'C
Cuts at: 0 289 615
Size: 289 326
BglI GCCn_nnn'nGGC
Cuts at: 0 13 615
Size: 13 602
BlpI GC'TnA_GC
Cuts at: 0 118 615
Size: 118 497
BsaHI Gr'CG_yC
Cuts at: 0 79 286 615
Size: 79 207 329
Fragments arranged by size:
329 207 79
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 322 352 615
Size: 322 30 263
Fragments arranged by size:
322 263 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 127 385 615
Size: 127 258 230
Fragments arranged by size:
258 230 127
BsiEI CG_ry'CG
Cuts at: 0 86 615
Size: 86 529
BsrFI r'CCGG_y
Cuts at: 0 23 615
Size: 23 592
BstYI r'GATC_y
Cuts at: 0 38 571 615
Size: 38 533 44
Fragments arranged by size:
533 44 38
BtgI C'CryG_G
Cuts at: 0 96 281 615
Size: 96 185 334
Fragments arranged by size:
334 185 96
BtsI GCAGTG_nn'
Cuts at: 0 369 615
Size: 369 246
EaeI y'GGCC_r
Cuts at: 0 83 615
Size: 83 532
EagI C'GGCC_G
Cuts at: 0 83 615
Size: 83 532
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 341 413 615
Size: 296 45 72 202
Fragments arranged by size:
296 202 72 45
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 57 236 615
Size: 57 179 379
Fragments arranged by size:
379 179 57
HaeII r_GCGC'y
Cuts at: 0 289 615
Size: 289 326
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 153 185 443 475 615
Size: 153 32 258 32 140
Fragments arranged by size:
258 153 140 32 32
HincII GTy'rAC
Cuts at: 0 108 615
Size: 108 507
HindIII A'AGCT_T
Cuts at: 0 433 615
Size: 433 182
KasI G'GCGC_C
Cuts at: 0 285 615
Size: 285 330
MslI CAynn'nnrTG
Cuts at: 0 443 500 615
Size: 443 57 115
Fragments arranged by size:
443 115 57
MspA1I CmG'CkG
Cuts at: 0 98 319 615
Size: 98 221 296
Fragments arranged by size:
296 221 98
NarI GG'CG_CC
Cuts at: 0 286 615
Size: 286 329
NcoI C'CATG_G
Cuts at: 0 281 615
Size: 281 334
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 40 72 615
Size: 40 32 543
Fragments arranged by size:
543 40 32
PstI C_TGCA'G
Cuts at: 0 318 534 615
Size: 318 216 81
Fragments arranged by size:
318 216 81
PvuII CAG'CTG
Cuts at: 0 319 615
Size: 319 296
SacII CC_GC'GG
Cuts at: 0 99 615
Size: 99 516
SalI G'TCGA_C
Cuts at: 0 106 615
Size: 106 509
SfcI C'TryA_G
Cuts at: 0 255 314 530 615
Size: 255 59 216 85
Fragments arranged by size:
255 216 85 59
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 13 615
Size: 13 602
SfoI GGC'GCC
Cuts at: 0 287 615
Size: 287 328
StyI C'CwwG_G
Cuts at: 0 281 615
Size: 281 334
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 279 615
Size: 279 336
Enzymes that do cut:
AccI AcuI AseI BanI BbeI BglI BlpI BsaHI
BsaXI BseRI BsiEI BsrFI BstYI BtgI BtsI EaeI
EagI Eco57MI FalI HaeII Hin4I HincII HindIII KasI
MslI MspA1I NarI NcoI PpiI PstI PvuII SacII
SalI SfcI SfiI SfoI StyI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AsiSI AvaI AvrII BaeI BamHI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglII Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaWI BseYI BsgI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EarI EciI EcoICRI
EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI HpaI
KpnI MfeI MluI MmeI MscI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SanDI SapI SbfI
ScaI SexAI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI