(Circular) (Six-base) MAPSORT of: pFN21AB6924.seq Check: 3928 from: 1 to: 5386 pFN21AB6924 5386 bp With 182 enzymes: SgfI * October 20, 2009 10:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3196 278 Size: 53 83 186 936 1660 2468 Fragments arranged by size: 2468 1660 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2729 1937 Size: 792 4594 Acc65I G'GTAC_C Cuts at: 2707 2707 Size: 5386 AclI AA'CG_TT Cuts at: 1563 3513 3886 1563 Size: 1950 373 3063 Fragments arranged by size: 3063 1950 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3449 4833 5101 1632 Size: 1817 1384 268 1917 Fragments arranged by size: 1917 1817 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5189 AflIII A'CryG_T Cuts at: 1203 4285 5262 1203 Size: 3082 977 1327 Fragments arranged by size: 3082 1327 977 AgeI A'CCGG_T Cuts at: 4217 4217 Size: 5386 AhdI GACnn_n'nnGTC Cuts at: 4115 4115 Size: 5386 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5386 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5386 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2567 2599 2567 Size: 32 5354 AlwNI CAG_nnn'CTG Cuts at: 3129 4701 3129 Size: 1572 3814 ApaI G_GGCC'C Cuts at: 2307 2307 Size: 5386 ApaLI G'TGCA_C Cuts at: 3443 4599 3443 Size: 1156 4230 ApoI r'AATT_y Cuts at: 1493 2695 2921 3073 5182 1493 Size: 1202 226 152 2109 1697 Fragments arranged by size: 2109 1697 1202 226 152 AseI AT'TA_AT Cuts at: 160 1032 3940 160 Size: 872 2908 1606 Fragments arranged by size: 2908 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5386 AvaI C'yCGr_G Cuts at: 1870 1943 2224 2711 1870 Size: 73 281 487 4545 Fragments arranged by size: 4545 487 281 73 BamHI G'GATC_C Cuts at: 2716 3083 2716 Size: 367 5019 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2427 2602 2707 4162 618 Size: 325 217 627 640 175 105 1455 1842 Fragments arranged by size: 1842 1455 640 627 325 217 175 105 BanII G_rGCy'C Cuts at: 1391 2307 2705 1391 Size: 916 398 4072 Fragments arranged by size: 4072 916 398 BbeI G_GCGC'C Cuts at: 2431 2431 Size: 5386 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4415 BbvCI CC'TCA_GC Cuts at: 2061 2061 Size: 5386 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2495 2529 3577 3611 1903 Size: 34 50 34 474 34 1048 34 3678 Fragments arranged by size: 3678 1048 474 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2236 3278 4488 2236 Size: 1042 1210 3134 Fragments arranged by size: 3134 1210 1042 BclI T'GATC_A Cuts at: 1548 1548 Size: 5386 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3997 136 Size: 107 122 71 631 605 2325 1525 Fragments arranged by size: 2325 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5381 5381 Size: 5386 BlpI GC'TnA_GC Cuts at: 2803 2803 Size: 5386 Bme1580I G_kGCm'C Cuts at: 1790 2307 2394 2559 2607 3447 4603 1790 Size: 517 87 165 48 840 1156 2573 Fragments arranged by size: 2573 1156 840 517 165 87 48 BmgBI CAC'GTC Cuts at: 1318 2554 1318 Size: 1236 4150 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3117 4075 442 Size: 416 53 83 399 24 1700 958 1753 Fragments arranged by size: 1753 1700 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5386 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2416 2546 4046 1239 Size: 1177 130 1500 2579 Fragments arranged by size: 2579 1500 1177 130 Bpu10I CC'TnA_GC Cuts at: 2061 2061 Size: 5386 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2158 3511 4376 4674 2158 Size: 1353 865 298 2870 Fragments arranged by size: 2870 1353 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4049 882 Size: 617 2550 2219 Fragments arranged by size: 2550 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3177 493 Size: 711 1973 2702 Fragments arranged by size: 2702 1973 711 BsaBI GATnn'nnATC Cuts at: 5380 5380 Size: 5386 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2261 2428 3193 3575 Size: 53 83 186 936 728 167 765 382 Cuts at: 3575 4210 275 Size: 635 1451 Fragments arranged by size: 1451 936 765 728 635 382 186 167 83 53 BsaWI w'CCGG_w Cuts at: 2230 2493 3819 4217 4491 4638 5049 2230 Size: 263 1326 398 274 147 411 2567 Fragments arranged by size: 2567 1326 411 398 274 263 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2567 2597 1178 Size: 30 165 30 1164 30 3967 Fragments arranged by size: 3967 1164 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2061 2543 2546 2660 1186 Size: 875 482 3 114 3912 Fragments arranged by size: 3912 875 482 114 3 BseYI C'CCAG_C Cuts at: 2598 4589 5085 2598 Size: 1991 496 2899 Fragments arranged by size: 2899 1991 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4813 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2123 2494 2836 3597 4625 1540 Size: 275 186 122 371 342 761 1028 2301 Fragments arranged by size: 2301 1028 761 371 342 275 186 122 BsiHKAI G_wGCw'C Cuts at: 3447 3532 4603 3447 Size: 85 1071 4230 Fragments arranged by size: 4230 1071 85 BsmI GAATG_Cn' Cuts at: 2081 2897 2990 2081 Size: 816 93 4477 Fragments arranged by size: 4477 816 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2307 2394 2559 2607 2705 3447 3532 Size: 399 517 87 165 48 98 742 85 Cuts at: 3532 4603 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 517 399 165 98 87 85 48 BspEI T'CCGG_A Cuts at: 5049 5049 Size: 5386 BspHI T'CATG_A Cuts at: 2544 3275 5005 2544 Size: 731 1730 2925 Fragments arranged by size: 2925 1730 731 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2741 844 Size: 1897 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3273 1394 Size: 429 1450 3507 Fragments arranged by size: 3507 1450 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3881 4055 65 Size: 1183 2633 174 1396 Fragments arranged by size: 2633 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4030 4217 1700 Size: 110 2220 187 2869 Fragments arranged by size: 2869 2220 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5386 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5386 BssSI C'ACGA_G Cuts at: 3446 4458 3446 Size: 1012 4374 BstAPI GCAn_nnn'nTGC Cuts at: 794 2281 794 Size: 1487 3899 BstBI TT'CG_AA Cuts at: 4190 4190 Size: 5386 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5386 BstYI r'GATC_y Cuts at: 1972 2716 3083 3468 3485 4937 5023 5035 5381 Size: 744 367 385 17 1452 86 12 346 Cuts at: 5381 1972 Size: 1977 Fragments arranged by size: 1977 1452 744 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2162 2665 513 Size: 552 1097 503 3234 Fragments arranged by size: 3234 1097 552 503 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2443 487 Size: 683 56 1217 3430 Fragments arranged by size: 3430 1217 683 56 BtsI GCAGTG_nn' Cuts at: 2902 3696 3716 2902 Size: 794 20 4572 Fragments arranged by size: 4572 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5386 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5351 DraI TTT'AAA Cuts at: 2690 2690 Size: 5386 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4393 809 Size: 507 3077 1802 Fragments arranged by size: 3077 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2361 2833 3722 Size: 54 1425 50 275 18 531 472 889 Cuts at: 3722 5351 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 531 472 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2833 1537 Size: 275 1021 4090 Fragments arranged by size: 4090 1021 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3316 1353 Size: 1963 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2087 2173 2210 2277 3973 4347 Size: 1064 138 498 86 37 67 1696 374 Cuts at: 4347 4493 387 Size: 146 1280 Fragments arranged by size: 1696 1280 1064 498 374 146 138 86 67 37 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2416 2546 3449 4046 4833 5101 1239 Size: 393 784 130 903 597 787 268 1524 Fragments arranged by size: 1524 903 787 784 597 393 268 130 EcoNI CCTnn'n_nnAGG Cuts at: 2528 2528 Size: 5386 EcoO109I rG'GnC_Cy Cuts at: 2499 2499 Size: 5386 EcoRI G'AATT_C Cuts at: 2695 3073 2695 Size: 378 5008 FspI TGC'GCA Cuts at: 2636 3892 2636 Size: 1256 4130 HaeII r_GCGC'y Cuts at: 1678 2431 4533 1678 Size: 753 2102 2531 Fragments arranged by size: 2531 2102 753 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4027 4059 4101 4133 1373 Size: 32 135 32 2455 32 42 32 2626 Fragments arranged by size: 2626 2455 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2730 2976 669 Size: 988 281 792 246 3079 Fragments arranged by size: 3079 988 792 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5386 HpaI GTT'AAC Cuts at: 2976 2976 Size: 5386 KasI G'GCGC_C Cuts at: 2427 2427 Size: 5386 KpnI G_GTAC'C Cuts at: 2711 2711 Size: 5386 MfeI C'AATT_G Cuts at: 2985 2985 Size: 5386 MluI A'CGCG_T Cuts at: 5262 5262 Size: 5386 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2103 4500 4684 1305 Size: 798 2397 184 2007 Fragments arranged by size: 2397 2007 798 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2363 10 Size: 54 1425 343 531 3033 Fragments arranged by size: 3033 1425 531 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2201 2543 3344 3703 3862 518 Size: 648 1035 342 801 359 159 2042 Fragments arranged by size: 2042 1035 801 648 359 342 159 MspA1I CmG'CkG Cuts at: 1606 2065 2164 2598 2802 3479 4627 4872 1606 Size: 459 99 434 204 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 459 434 245 204 99 NarI GG'CG_CC Cuts at: 2428 2428 Size: 5386 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4834 NdeI CA'TA_TG Cuts at: 387 387 Size: 5386 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5386 NotI GC'GGCC_GC Cuts at: 2833 2833 Size: 5386 NspI r_CATG'y Cuts at: 1132 1229 2284 2359 2744 4289 1132 Size: 97 1055 75 385 1545 2229 Fragments arranged by size: 2229 1545 1055 385 97 75 PasI CC'CwG_GG Cuts at: 1348 2091 1348 Size: 743 4643 PciI A'CATG_T Cuts at: 4285 4285 Size: 5386 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5386 PfoI T'CCnGG_A Cuts at: 2142 2142 Size: 5386 PmeI GTTT'AAAC Cuts at: 2690 2690 Size: 5386 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2567 2599 3455 3487 4968 5000 2567 Size: 32 856 32 1481 32 2953 Fragments arranged by size: 2953 1481 856 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2499 2499 Size: 5386 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5386 PsiI TTA'TAA Cuts at: 2956 2956 Size: 5386 PspOMI G'GGCC_C Cuts at: 2303 2303 Size: 5386 PstI C_TGCA'G Cuts at: 830 2070 2738 830 Size: 1240 668 3478 Fragments arranged by size: 3478 1240 668 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5386 PvuII CAG'CTG Cuts at: 2065 2065 Size: 5386 SacII CC_GC'GG Cuts at: 2165 2165 Size: 5386 SalI G'TCGA_C Cuts at: 1936 2728 1936 Size: 792 4594 SbfI CC_TGCA'GG Cuts at: 2738 2738 Size: 5386 ScaI AGT'ACT Cuts at: 3634 5346 3634 Size: 1712 3674 SfcI C'TryA_G Cuts at: 826 1045 2066 2350 2734 3869 4550 4741 826 Size: 219 1021 284 384 1135 681 191 1471 Fragments arranged by size: 1471 1135 1021 681 384 284 219 191 SfoI GGC'GCC Cuts at: 2429 2429 Size: 5386 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5386 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5386 SmaI CCC'GGG Cuts at: 1872 2226 2713 1872 Size: 354 487 4545 Fragments arranged by size: 4545 487 354 SmlI C'TyrA_G Cuts at: 820 1017 1943 2173 3490 4391 4653 820 Size: 197 926 230 1317 901 262 1553 Fragments arranged by size: 1553 1317 926 901 262 230 197 SnaBI TAC'GTA Cuts at: 493 3177 493 Size: 2684 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5386 SphI G_CATG'C Cuts at: 1132 2359 2744 1132 Size: 1227 385 3774 Fragments arranged by size: 3774 1227 385 SspI AAT'ATT Cuts at: 5 52 3310 5 Size: 47 3258 2081 Fragments arranged by size: 3258 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5386 StyI C'CwwG_G Cuts at: 513 1065 2015 2825 513 Size: 552 950 810 3074 Fragments arranged by size: 3074 950 810 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2590 3413 3430 3583 3768 4213 325 Size: 1255 1010 823 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1010 823 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3632 5344 96 Size: 274 80 33 51 1443 1655 1712 138 Fragments arranged by size: 1712 1655 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2220 2252 5346 5378 2220 Size: 32 3094 32 2228 Fragments arranged by size: 3094 2228 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5386 XbaI T'CTAG_A Cuts at: 2722 2722 Size: 5386 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2194 2194 Size: 5386 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5386 XmaI C'CCGG_G Cuts at: 1870 2224 2711 1870 Size: 354 487 4545 Fragments arranged by size: 4545 487 354 XmnI GAAnn'nnTTC Cuts at: 3515 3515 Size: 5386 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3194 276 Size: 53 83 186 936 1660 2468 Fragments arranged by size: 2468 1660 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI