Restriction map for
pFN21AB6921
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# NO CUT ENZYMES ( 159 enzymes) #
##################################
AatII Acc65I AccIII AclI AfaI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BanII BbrPI BbvCI BcgI BclI
BcnI BfaI BfrI BglI BglII
BlnI BlpI BmyI Bpu1102I BsaAI
BsaWI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DdeI DraI DraII
DraIII EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI Ksp632I MaeI MaeII
MaeIII MfeI MluI MluNI MroI
MscI MunI NciI NcoI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tsp45I Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AccII 50 CGCG CG^CG
AlwNI 69 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 525 GAACGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 220 GGACC G^G[AT]CC
BamHI 543 GGATCC G^GATCC
BanI 562 GGCGCC G^G[CT][GA]CC
BbeI 562 GGCGCC GGCGC^C
BbsI 157 GTCTTC GAAGAC(2/6)
BciVI 132 GTATCC GTATCC(6/5)
BpuAI 157 GTCTTC GAAGAC(2/6)
BsaBI 183 GATACCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 207 GAGACC GGTCTC(1/5)
BsgI 401 GTGCAG GTGCAG(16/14)
BsmFI 53 GGGAC GGGAC(10/14)
BsrFI 36 GCCGGC [GA]^CCGG[CT]
BssKI 211 CCTGG ^CC[ACGT]GG
BstF5I 556 GGATG GGATG(2/0)
BstNI 211 CCTGG CC^[AT]GG
BstUI 50 CGCG CG^CG
BstYI 543 GGATCC [GA]^GATC[CT]
CfoI 563 GCGC GCG^C
Cfr10I 36 GCCGGC [GA]^CCGG[CT]
DrdI 55 GACGCCTGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 49 CCGCGG C^C[GA][CT]GG
EaeI 38 CGGCCA [CT]^GGCC[GA]
EcoRII 211 CCTGG ^CC[AT]GG
EcoT14I 548 CCAAGG C^C[AT][AT]GG
FokI 556 GGATG GGATG(9/13)
HaeII 562 GGCGCC [GA]GCGC^[CT]
HapII 37 CCGG C^CGG
HhaI 563 GCGC GCG^C
HinP1I 563 GCGC G^CGC
HpaII 37 CCGG C^CGG
HphI 366 TCACC GGTGA(8/7)
KasI 562 GGCGCC G^GCGCC
KspI 49 CCGCGG CCGC^GG
MamI 183 GATACCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 543 GGATCC [GA]^GATC[CT]
MseI 347 TTAA T^TAA
MslI 534 CATCAGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 37 CCGG C^CGG
MvaI 211 CCTGG CC^[AT]GG
MvnI 50 CGCG CG^CG
NaeI 36 GCCGGC GCC^GGC
NarI 562 GGCGCC GG^CGCC
NgoMIV 36 GCCGGC G^CCGGC
PleI 451 GACTC GAGTC(4/5)
PvuII 72 CAGCTG CAG^CTG
SacII 49 CCGCGG CCGC^GG
ScrFI 211 CCTGG CC^[ACGT]GG
SfaNI 340 GATGC GCATC(5/9)
SfcI 66 CTACAG C^T[GA][CT]AG
SfoI 562 GGCGCC GGC^GCC
SspI 91 AATATT AAT^ATT
StyI 548 CCAAGG C^C[AT][AT]GG
TaqI 293 TCGA T^CGA
Tru9I 347 TTAA T^TAA
TspRI 456 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 293 TCGA T^CGA
XhoII 543 GGATCC [GA]^GATC[CT]
XmnI 525 GAACGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AccI 64 GTCTAC GT^[AC][GT]AC
568 GTCTAC
AcsI 385 AAATTT [GA]^AATT[CT]
583 GAATTT
AcyI 55 GACGCC G[GA]^CG[CT]C
562 GGCGCC
AluI 73 AGCT AG^CT
101 AGCT
ApoI 385 AAATTT [GA]^AATT[CT]
583 GAATTT
BsaHI 55 GACGCC G[GA]^CG[CT]C
562 GGCGCC
BsaJI 49 CCGCGG C^C[ACGT][ACGT]GG
548 CCAAGG
BsmAI 207 GAGAC GTCTC(1/5)
499 GAGAC
Cfr13I 13 GGCCC G^G[ACGT]CC
220 GGACC
HaeIII 13 GGCC GG^CC
39 GGCC
HgaI 55 GACGC GACGC(5/10)
62 GCGTC
Hin1I 55 GACGCC G[GA]^CG[CT]C
562 GGCGCC
MwoI 62 GCGTCTACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
474 GCCTCCGCCGC
Sau96I 13 GGCCC G^G[ACGT]CC
220 GGACC
TfiI 406 GATTC G^A[AT]TC
508 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AciI AlwI BbvI BpmI BsiYI
BslI BsrI Cac8I DpnII FauI
Fnu4HI HinfI ItaI MboI MboII
MnlI MspA1I NdeII NlaIII NlaIV
Sau3AI TseI Tsp509I