(Circular) (Six-base) MAPSORT of: pFN21AB6921.seq Check: 2885 from: 1 to: 5320 pFN21AB6921 5320 bp With 182 enzymes: SgfI * October 20, 2009 10:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3130 278 Size: 53 83 186 936 1594 2468 Fragments arranged by size: 2468 1594 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2070 2574 2663 1937 Size: 133 504 89 4594 Fragments arranged by size: 4594 504 133 89 Acc65I G'GTAC_C Cuts at: 2641 2641 Size: 5320 AclI AA'CG_TT Cuts at: 1563 3447 3820 1563 Size: 1884 373 3063 Fragments arranged by size: 3063 1884 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3383 4767 5035 1632 Size: 1751 1384 268 1917 Fragments arranged by size: 1917 1751 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5123 AflIII A'CryG_T Cuts at: 1203 4219 5196 1203 Size: 3016 977 1327 Fragments arranged by size: 3016 1327 977 AgeI A'CCGG_T Cuts at: 4151 4151 Size: 5320 AhdI GACnn_n'nnGTC Cuts at: 4049 4049 Size: 5320 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5320 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5320 AlwNI CAG_nnn'CTG Cuts at: 2079 3063 4635 2079 Size: 984 1572 2764 Fragments arranged by size: 2764 1572 984 ApaLI G'TGCA_C Cuts at: 3377 4533 3377 Size: 1156 4164 ApoI r'AATT_y Cuts at: 1493 2390 2588 2629 2855 3007 5116 1493 Size: 897 198 41 226 152 2109 1697 Fragments arranged by size: 2109 1697 897 226 198 152 41 AseI AT'TA_AT Cuts at: 160 1032 3874 160 Size: 872 2842 1606 Fragments arranged by size: 2842 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5320 AvaI C'yCGr_G Cuts at: 1870 1943 2645 1870 Size: 73 702 4545 Fragments arranged by size: 4545 702 73 BamHI G'GATC_C Cuts at: 2548 2650 3017 2548 Size: 102 367 4851 Fragments arranged by size: 4851 367 102 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2567 2641 4096 618 Size: 325 217 627 780 74 1455 1842 Fragments arranged by size: 1842 1455 780 627 325 217 74 BanII G_rGCy'C Cuts at: 1391 2639 1391 Size: 1248 4072 BbeI G_GCGC'C Cuts at: 2571 2571 Size: 5320 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2155 928 Size: 971 256 4093 Fragments arranged by size: 4093 971 256 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3511 3545 1903 Size: 34 1574 34 3678 Fragments arranged by size: 3678 1574 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2148 3212 4422 2148 Size: 1064 1210 3046 Fragments arranged by size: 3046 1210 1064 BclI T'GATC_A Cuts at: 1548 1548 Size: 5320 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3931 136 Size: 107 122 71 631 605 2259 1525 Fragments arranged by size: 2259 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5315 5315 Size: 5320 BlpI GC'TnA_GC Cuts at: 2737 2737 Size: 5320 Bme1580I G_kGCm'C Cuts at: 1790 3381 4537 1790 Size: 1591 1156 2573 Fragments arranged by size: 2573 1591 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5320 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3051 4009 442 Size: 416 53 83 399 24 1634 958 1753 Fragments arranged by size: 1753 1634 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5320 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2496 2496 Size: 5320 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2238 2443 2529 3980 1239 Size: 999 205 86 1451 2579 Fragments arranged by size: 2579 1451 999 205 86 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3445 4310 4608 3445 Size: 865 298 4157 Fragments arranged by size: 4157 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2206 3983 882 Size: 617 707 1777 2219 Fragments arranged by size: 2219 1777 707 617 BsaAI yAC'GTr Cuts at: 493 1204 3111 493 Size: 711 1907 2702 Fragments arranged by size: 2702 1907 711 BsaBI GATnn'nnATC Cuts at: 2192 5314 2192 Size: 3122 2198 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2061 2568 3127 3509 Size: 53 83 186 936 528 507 559 382 Cuts at: 3509 4144 275 Size: 635 1451 Fragments arranged by size: 1451 936 635 559 528 507 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3753 4151 4425 4572 4983 3753 Size: 398 274 147 411 4090 Fragments arranged by size: 4090 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5095 Fragments arranged by size: 5095 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5320 BseYI C'CCAG_C Cuts at: 4523 5019 4523 Size: 496 4824 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2427 1163 Size: 573 691 4056 Fragments arranged by size: 4056 691 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2770 3531 4559 1540 Size: 275 186 769 761 1028 2301 Fragments arranged by size: 2301 1028 769 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3381 3466 4537 3381 Size: 85 1071 4164 Fragments arranged by size: 4164 1071 85 BsmI GAATG_Cn' Cuts at: 2831 2924 2831 Size: 93 5227 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2639 3381 3466 4537 1391 Size: 399 849 742 85 1071 2174 Fragments arranged by size: 2174 1071 849 742 399 85 BspEI T'CCGG_A Cuts at: 4983 4983 Size: 5320 BspHI T'CATG_A Cuts at: 3209 4939 3209 Size: 1730 3590 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2675 844 Size: 1831 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3207 1394 Size: 429 1384 3507 Fragments arranged by size: 3507 1384 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3815 3989 65 Size: 1183 2567 174 1396 Fragments arranged by size: 2567 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2041 3964 4151 1700 Size: 110 231 1923 187 2869 Fragments arranged by size: 2869 1923 231 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5320 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5320 BssSI C'ACGA_G Cuts at: 3380 4392 3380 Size: 1012 4308 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5320 BstBI TT'CG_AA Cuts at: 4124 4124 Size: 5320 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5320 BstYI r'GATC_y Cuts at: 1972 2548 2650 3017 3402 3419 4871 4957 4969 Size: 576 102 367 385 17 1452 86 12 Cuts at: 4969 5315 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 576 385 367 346 102 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2054 513 Size: 552 939 50 3779 Fragments arranged by size: 3779 939 552 50 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4581 Fragments arranged by size: 4581 683 56 BtsI GCAGTG_nn' Cuts at: 2836 3630 3650 2836 Size: 794 20 4506 Fragments arranged by size: 4506 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5320 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5285 DraI TTT'AAA Cuts at: 2624 2624 Size: 5320 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2066 4327 809 Size: 507 750 2261 1802 Fragments arranged by size: 2261 1802 750 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2043 2767 3656 Size: 54 1425 50 275 18 213 724 889 Cuts at: 3656 5285 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 724 275 213 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2767 1537 Size: 275 955 4090 Fragments arranged by size: 4090 955 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3250 1353 Size: 1897 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2472 3907 4281 4427 387 Size: 1064 138 883 1435 374 146 1280 Fragments arranged by size: 1435 1280 1064 883 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2238 2443 2529 3383 3980 4767 5035 Size: 393 606 205 86 854 597 787 268 Cuts at: 5035 1239 Size: 1524 Fragments arranged by size: 1524 854 787 606 597 393 268 205 86 EcoRI G'AATT_C Cuts at: 2629 3007 2629 Size: 378 4942 FspI TGC'GCA Cuts at: 3826 3826 Size: 5320 HaeII r_GCGC'y Cuts at: 1678 2571 4467 1678 Size: 893 1896 2531 Fragments arranged by size: 2531 1896 893 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2217 2249 3961 3993 4035 Size: 32 135 32 645 32 1712 32 42 Cuts at: 4035 4067 1373 Size: 32 2626 Fragments arranged by size: 2626 1712 645 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2664 2910 669 Size: 988 281 726 246 3079 Fragments arranged by size: 3079 988 726 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5320 HpaI GTT'AAC Cuts at: 2910 2910 Size: 5320 KasI G'GCGC_C Cuts at: 2567 2567 Size: 5320 KpnI G_GTAC'C Cuts at: 2645 2645 Size: 5320 MfeI C'AATT_G Cuts at: 2919 2919 Size: 5320 MluI A'CGCG_T Cuts at: 5196 5196 Size: 5320 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4434 4618 1305 Size: 3129 184 2007 Fragments arranged by size: 3129 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3498 Fragments arranged by size: 3498 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2543 3278 3637 3796 518 Size: 648 1377 735 359 159 2042 Fragments arranged by size: 2042 1377 735 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2024 2056 2079 2736 3413 4561 4806 1606 Size: 418 32 23 657 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 657 418 245 32 23 NaeI GCC'GGC Cuts at: 2043 2043 Size: 5320 NarI GG'CG_CC Cuts at: 2568 2568 Size: 5320 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3829 Fragments arranged by size: 3829 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5320 NgoMIV G'CCGG_C Cuts at: 2041 2041 Size: 5320 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5320 NotI GC'GGCC_GC Cuts at: 2767 2767 Size: 5320 NspI r_CATG'y Cuts at: 1132 1229 2678 4223 1132 Size: 97 1449 1545 2229 Fragments arranged by size: 2229 1545 1449 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5320 PciI A'CATG_T Cuts at: 4219 4219 Size: 5320 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5320 PmeI GTTT'AAAC Cuts at: 2624 2624 Size: 5320 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3389 3421 4902 4934 3389 Size: 32 1481 32 3775 Fragments arranged by size: 3775 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5320 PsiI TTA'TAA Cuts at: 2890 2890 Size: 5320 PstI C_TGCA'G Cuts at: 830 2672 830 Size: 1842 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5320 PvuII CAG'CTG Cuts at: 2079 2079 Size: 5320 SacII CC_GC'GG Cuts at: 2057 2057 Size: 5320 SalI G'TCGA_C Cuts at: 1936 2662 1936 Size: 726 4594 SbfI CC_TGCA'GG Cuts at: 2672 2672 Size: 5320 ScaI AGT'ACT Cuts at: 3568 5280 3568 Size: 1712 3608 SfcI C'TryA_G Cuts at: 826 1045 2071 2668 3803 4484 4675 826 Size: 219 1026 597 1135 681 191 1471 Fragments arranged by size: 1471 1135 1026 681 597 219 191 SfoI GGC'GCC Cuts at: 2569 2569 Size: 5320 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5320 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5320 SmaI CCC'GGG Cuts at: 1872 2647 1872 Size: 775 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3424 4325 4587 820 Size: 197 926 1481 901 262 1553 Fragments arranged by size: 1553 1481 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3111 493 Size: 2618 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5320 SphI G_CATG'C Cuts at: 1132 2678 1132 Size: 1546 3774 SspI AAT'ATT Cuts at: 5 52 2098 3244 5 Size: 47 2046 1146 2081 Fragments arranged by size: 2081 2046 1146 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5320 StyI C'CwwG_G Cuts at: 513 1065 2004 2553 2759 513 Size: 552 939 549 206 3074 Fragments arranged by size: 3074 939 552 549 206 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2388 2454 3347 3364 3517 3702 4147 Size: 1255 808 66 893 17 153 185 445 Cuts at: 4147 325 Size: 1498 Fragments arranged by size: 1498 1255 893 808 445 185 153 66 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3566 5278 96 Size: 274 80 33 51 1443 1589 1712 138 Fragments arranged by size: 1712 1589 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2095 2127 5280 5312 2095 Size: 32 3153 32 2103 Fragments arranged by size: 3153 2103 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5320 XbaI T'CTAG_A Cuts at: 2656 2656 Size: 5320 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5320 XmaI C'CCGG_G Cuts at: 1870 2645 1870 Size: 775 4545 XmnI GAAnn'nnTTC Cuts at: 2534 3449 2534 Size: 915 4405 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3128 276 Size: 53 83 186 936 1594 2468 Fragments arranged by size: 2468 1594 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI