(Linear) (Six-base) MAPSORT of: KIBB6921.seq  Check: 3022  from: 1  to: 615



KIBB6921.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AccI GT'mk_AC

Cuts at:      0      65     569     615 
   Size:         65     504      46

  Fragments arranged by size: 

                 504      65      46
 
AlwNI CAG_nnn'CTG

Cuts at:      0      74     615 
   Size:         74     541
 
ApoI r'AATT_y

Cuts at:      0     385     583     615 
   Size:        385     198      32

  Fragments arranged by size: 

                 385     198      32
 
BamHI G'GATC_C

Cuts at:      0     543     615 
   Size:        543      72
 
BanI G'GyrC_C

Cuts at:      0     562     615 
   Size:        562      53
 
BbeI G_GCGC'C

Cuts at:      0     566     615 
   Size:        566      49
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     150     615 
   Size:        150     465
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     143     615 
   Size:        143     472
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     491     615 
   Size:        491     124
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     233     438     524     615 
   Size:        233     205      86      91

  Fragments arranged by size: 

                 233     205      91      86
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     201     615 
   Size:        201     414
 
BsaBI GATnn'nnATC

Cuts at:      0     187     615 
   Size:        187     428
 
BsaHI Gr'CG_yC

Cuts at:      0      56     563     615 
   Size:         56     507      52

  Fragments arranged by size: 

                 507      56      52
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     422     615 
   Size:        422     193
 
BsrFI r'CCGG_y

Cuts at:      0      36     615 
   Size:         36     579
 
BstYI r'GATC_y

Cuts at:      0     543     615 
   Size:        543      72
 
BtgI C'CryG_G

Cuts at:      0      49     615 
   Size:         49     566
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      61     615 
   Size:         61     554
 
EaeI y'GGCC_r

Cuts at:      0      38     615 
   Size:         38     577
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     467     615 
   Size:        467     148
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     233     438     524     615 
   Size:        233     205      86      91

  Fragments arranged by size: 

                 233     205      91      86
 
HaeII r_GCGC'y

Cuts at:      0     566     615 
   Size:        566      49
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     212     244     615 
   Size:        212      32     371

  Fragments arranged by size: 

                 371     212      32
 
KasI G'GCGC_C

Cuts at:      0     562     615 
   Size:        562      53
 
MslI CAynn'nnrTG

Cuts at:      0     538     615 
   Size:        538      77
 
MspA1I CmG'CkG

Cuts at:      0      19      51      74     615 
   Size:         19      32      23     541

  Fragments arranged by size: 

                 541      32      23      19
 
NaeI GCC'GGC

Cuts at:      0      38     615 
   Size:         38     577
 
NarI GG'CG_CC

Cuts at:      0     563     615 
   Size:        563      52
 
NgoMIV G'CCGG_C

Cuts at:      0      36     615 
   Size:         36     579
 
PvuII CAG'CTG

Cuts at:      0      74     615 
   Size:         74     541
 
SacII CC_GC'GG

Cuts at:      0      52     615 
   Size:         52     563
 
SfcI C'TryA_G

Cuts at:      0      66     615 
   Size:         66     549
 
SfoI GGC'GCC

Cuts at:      0     564     615 
   Size:        564      51
 
SspI AAT'ATT

Cuts at:      0      93     615 
   Size:         93     522
 
StyI C'CwwG_G

Cuts at:      0     548     615 
   Size:        548      67
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     383     449     615 
   Size:        383      66     166

  Fragments arranged by size: 

                 383     166      66
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      90     122     615 
   Size:         90      32     493

  Fragments arranged by size: 

                 493      90      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     529     615 
   Size:        529      86
 

 Enzymes that do cut:

     AccI    AlwNI     ApoI    BamHI     BanI     BbeI     BbsI    BciVI
     BplI     BpmI     BsaI    BsaBI    BsaHI     BsgI    BsrFI    BstYI
     BtgI     DrdI     EaeI     EciI  Eco57MI    HaeII    Hin4I     KasI
     MslI   MspA1I     NaeI     NarI   NgoMIV    PvuII    SacII     SfcI
     SfoI     SspI     StyI    TaqII     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BanII    BbvCI     BcgI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI   Bpu10I    BpuEI    BsaAI    BsaWI    BsaXI    BseRI    BseYI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     EagI     EarI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     MscI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI     TatI
  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI