(Circular) (Six-base) MAPSORT of: pFN21AB6919.seq Check: 3045 from: 1 to: 5356 pFN21AB6919 5356 bp With 182 enzymes: SgfI * October 20, 2009 10:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2476 2476 Size: 5356 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3166 278 Size: 53 83 186 936 1630 2468 Fragments arranged by size: 2468 1630 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2699 1937 Size: 762 4594 Acc65I G'GTAC_C Cuts at: 2058 2677 2058 Size: 619 4737 AclI AA'CG_TT Cuts at: 1563 3483 3856 1563 Size: 1920 373 3063 Fragments arranged by size: 3063 1920 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3419 4803 5071 1632 Size: 1787 1384 268 1917 Fragments arranged by size: 1917 1787 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5159 AflIII A'CryG_T Cuts at: 1203 4255 5232 1203 Size: 3052 977 1327 Fragments arranged by size: 3052 1327 977 AgeI A'CCGG_T Cuts at: 4187 4187 Size: 5356 AhdI GACnn_n'nnGTC Cuts at: 4085 4085 Size: 5356 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5356 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5356 AlwNI CAG_nnn'CTG Cuts at: 2027 3099 4671 2027 Size: 1072 1572 2712 Fragments arranged by size: 2712 1572 1072 ApaLI G'TGCA_C Cuts at: 3413 4569 3413 Size: 1156 4200 ApoI r'AATT_y Cuts at: 1493 2665 2891 3043 5152 1493 Size: 1172 226 152 2109 1697 Fragments arranged by size: 2109 1697 1172 226 152 AseI AT'TA_AT Cuts at: 160 1032 3910 160 Size: 872 2878 1606 Fragments arranged by size: 2878 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5356 AvaI C'yCGr_G Cuts at: 1870 1943 2681 1870 Size: 73 738 4545 Fragments arranged by size: 4545 738 73 BamHI G'GATC_C Cuts at: 2046 2686 3053 2046 Size: 640 367 4349 Fragments arranged by size: 4349 640 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2058 2677 4132 618 Size: 325 217 627 271 619 1455 1842 Fragments arranged by size: 1842 1455 627 619 325 271 217 BanII G_rGCy'C Cuts at: 1391 2346 2675 1391 Size: 955 329 4072 Fragments arranged by size: 4072 955 329 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2156 928 Size: 971 257 4128 Fragments arranged by size: 4128 971 257 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3547 3581 1903 Size: 34 1610 34 3678 Fragments arranged by size: 3678 1610 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2567 3248 4458 2567 Size: 681 1210 3465 Fragments arranged by size: 3465 1210 681 BclI T'GATC_A Cuts at: 1548 1548 Size: 5356 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3967 136 Size: 107 122 71 631 605 2295 1525 Fragments arranged by size: 2295 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2211 5351 2211 Size: 3140 2216 BlpI GC'TnA_GC Cuts at: 2340 2773 2340 Size: 433 4923 Bme1580I G_kGCm'C Cuts at: 1790 2365 3417 4573 1790 Size: 575 1052 1156 2573 Fragments arranged by size: 2573 1156 1052 575 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5356 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2138 3087 4045 Size: 416 53 83 399 24 721 949 958 Cuts at: 4045 442 Size: 1753 Fragments arranged by size: 1753 958 949 721 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5356 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2177 2454 4016 1239 Size: 938 277 1562 2579 Fragments arranged by size: 2579 1562 938 277 Bpu10I CC'TnA_GC Cuts at: 2298 2298 Size: 5356 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2330 3481 4346 4644 2330 Size: 1151 865 298 3042 Fragments arranged by size: 3042 1151 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4019 882 Size: 617 2520 2219 Fragments arranged by size: 2520 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3147 493 Size: 711 1943 2702 Fragments arranged by size: 2702 1943 711 BsaBI GATnn'nnATC Cuts at: 5350 5350 Size: 5356 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3163 3545 4180 275 Size: 53 83 186 936 1630 382 635 1451 Fragments arranged by size: 1630 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3789 4187 4461 4608 5019 3789 Size: 398 274 147 411 4126 Fragments arranged by size: 4126 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5131 Fragments arranged by size: 5131 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5356 BseYI C'CCAG_C Cuts at: 4559 5055 4559 Size: 496 4860 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2324 1163 Size: 573 588 4195 Fragments arranged by size: 4195 588 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2806 3567 4595 1540 Size: 275 186 805 761 1028 2301 Fragments arranged by size: 2301 1028 805 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2346 3417 3502 4573 2346 Size: 1071 85 1071 3129 Fragments arranged by size: 3129 1071 1071 85 BsmI GAATG_Cn' Cuts at: 2417 2867 2960 2417 Size: 450 93 4813 Fragments arranged by size: 4813 450 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2612 2612 Size: 5356 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2346 2365 2675 3417 3502 4573 1391 Size: 399 556 19 310 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 556 399 310 85 19 BspEI T'CCGG_A Cuts at: 5019 5019 Size: 5356 BspHI T'CATG_A Cuts at: 3245 4975 3245 Size: 1730 3626 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2070 2476 2711 844 Size: 1226 406 235 3489 Fragments arranged by size: 3489 1226 406 235 BsrBI CCG'CTC Cuts at: 1394 1823 3243 1394 Size: 429 1420 3507 Fragments arranged by size: 3507 1420 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3851 4025 65 Size: 1183 2603 174 1396 Fragments arranged by size: 2603 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2076 4000 4187 1700 Size: 110 266 1924 187 2869 Fragments arranged by size: 2869 1924 266 187 110 BsrGI T'GTAC_A Cuts at: 96 2490 96 Size: 2394 2962 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5356 BssSI C'ACGA_G Cuts at: 3416 4428 3416 Size: 1012 4344 BstAPI GCAn_nnn'nTGC Cuts at: 794 2027 2311 794 Size: 1233 284 3839 Fragments arranged by size: 3839 1233 284 BstBI TT'CG_AA Cuts at: 4160 4160 Size: 5356 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5356 BstYI r'GATC_y Cuts at: 1972 2046 2211 2686 3053 3438 3455 4907 4993 Size: 74 165 475 367 385 17 1452 86 Cuts at: 4993 5005 5351 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 475 385 367 346 165 86 74 17 12 Bsu36I CC'TnA_GG Cuts at: 2054 2054 Size: 5356 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4804 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4617 Fragments arranged by size: 4617 683 56 BtsI GCAGTG_nn' Cuts at: 2292 2872 3666 3686 2292 Size: 580 794 20 3962 Fragments arranged by size: 3962 794 580 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5356 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5321 DraI TTT'AAA Cuts at: 2660 2660 Size: 5356 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2264 4363 809 Size: 507 948 2099 1802 Fragments arranged by size: 2099 1802 948 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2803 3692 5321 Size: 54 1425 50 275 18 973 889 1629 Cuts at: 5321 8 Size: 43 Fragments arranged by size: 1629 1425 973 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2803 1537 Size: 275 991 4090 Fragments arranged by size: 4090 991 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2469 2522 2528 3286 1353 Size: 1116 53 6 758 3423 Fragments arranged by size: 3423 1116 758 53 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3943 4317 4463 387 Size: 1064 138 2354 374 146 1280 Fragments arranged by size: 2354 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2344 2344 Size: 5356 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2177 2454 3419 4016 4803 5071 1239 Size: 393 545 277 965 597 787 268 1524 Fragments arranged by size: 1524 965 787 597 545 393 277 268 EcoRI G'AATT_C Cuts at: 2665 3043 2665 Size: 378 4978 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2614 2614 Size: 5356 FspI TGC'GCA Cuts at: 3862 3862 Size: 5356 HaeII r_GCGC'y Cuts at: 1678 4503 1678 Size: 2825 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3997 4029 4071 4103 1373 Size: 32 135 32 2425 32 42 32 2626 Fragments arranged by size: 2626 2425 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2700 2946 669 Size: 988 281 762 246 3079 Fragments arranged by size: 3079 988 762 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5356 HpaI GTT'AAC Cuts at: 2946 2946 Size: 5356 KpnI G_GTAC'C Cuts at: 2062 2681 2062 Size: 619 4737 MfeI C'AATT_G Cuts at: 2955 2955 Size: 5356 MluI A'CGCG_T Cuts at: 5232 5232 Size: 5356 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4470 4654 1305 Size: 3165 184 2007 Fragments arranged by size: 3165 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3534 Fragments arranged by size: 3534 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3314 3673 3832 518 Size: 648 2148 359 159 2042 Fragments arranged by size: 2148 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2116 2164 2772 3449 4597 4842 1606 Size: 510 48 608 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 608 510 245 48 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4804 NdeI CA'TA_TG Cuts at: 387 387 Size: 5356 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5356 NotI GC'GGCC_GC Cuts at: 2803 2803 Size: 5356 NspI r_CATG'y Cuts at: 1132 1229 2714 4259 1132 Size: 97 1485 1545 2229 Fragments arranged by size: 2229 1545 1485 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5356 PciI A'CATG_T Cuts at: 4255 4255 Size: 5356 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5356 PfoI T'CCnGG_A Cuts at: 2394 2394 Size: 5356 PmeI GTTT'AAAC Cuts at: 2660 2660 Size: 5356 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3425 3457 4938 4970 3425 Size: 32 1481 32 3811 Fragments arranged by size: 3811 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5356 PsiI TTA'TAA Cuts at: 2926 2926 Size: 5356 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2066 2098 2066 Size: 32 5324 PstI C_TGCA'G Cuts at: 830 2067 2708 830 Size: 1237 641 3478 Fragments arranged by size: 3478 1237 641 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5356 PvuII CAG'CTG Cuts at: 2164 2164 Size: 5356 SacI G_AGCT'C Cuts at: 2346 2346 Size: 5356 SalI G'TCGA_C Cuts at: 1936 2698 1936 Size: 762 4594 SbfI CC_TGCA'GG Cuts at: 2067 2708 2067 Size: 641 4715 ScaI AGT'ACT Cuts at: 2510 3604 5316 2510 Size: 1094 1712 2550 Fragments arranged by size: 2550 1712 1094 SfcI C'TryA_G Cuts at: 826 1045 2063 2083 2111 2422 2544 2704 3839 Size: 219 1018 20 28 311 122 160 1135 Cuts at: 3839 4520 4711 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1018 681 311 219 191 160 122 28 20 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5356 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5356 SmaI CCC'GGG Cuts at: 1872 2683 1872 Size: 811 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2345 3460 4361 4623 820 Size: 197 926 402 1115 901 262 1553 Fragments arranged by size: 1553 1115 926 901 402 262 197 SnaBI TAC'GTA Cuts at: 493 3147 493 Size: 2654 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5356 SphI G_CATG'C Cuts at: 1132 2714 1132 Size: 1582 3774 SspI AAT'ATT Cuts at: 5 52 3280 5 Size: 47 3228 2081 Fragments arranged by size: 3228 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5356 StyI C'CwwG_G Cuts at: 513 1065 2795 513 Size: 552 1730 3074 Fragments arranged by size: 3074 1730 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3383 3400 3553 3738 4183 325 Size: 1255 1803 17 153 185 445 1498 Fragments arranged by size: 1803 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2490 2508 3602 Size: 274 80 33 51 1443 513 18 1094 Cuts at: 3602 5314 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1094 513 274 138 80 51 33 18 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5316 5348 5316 Size: 32 5324 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5356 XbaI T'CTAG_A Cuts at: 2692 2692 Size: 5356 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5356 XmaI C'CCGG_G Cuts at: 1870 2681 1870 Size: 811 4545 XmnI GAAnn'nnTTC Cuts at: 2594 3485 2594 Size: 891 4465 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3164 276 Size: 53 83 186 936 1630 2468 Fragments arranged by size: 2468 1630 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI