(Circular) (Six-base) MAPSORT of: pFN21AB6917.seq Check: 3298 from: 1 to: 5320 pFN21AB6917 5320 bp With 182 enzymes: SgfI * October 20, 2009 10:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2529 2529 Size: 5320 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3130 278 Size: 53 83 186 936 1594 2468 Fragments arranged by size: 2468 1594 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2229 2663 1937 Size: 292 434 4594 Fragments arranged by size: 4594 434 292 Acc65I G'GTAC_C Cuts at: 2641 2641 Size: 5320 AclI AA'CG_TT Cuts at: 1563 3447 3820 1563 Size: 1884 373 3063 Fragments arranged by size: 3063 1884 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2463 3383 4767 5035 1632 Size: 831 920 1384 268 1917 Fragments arranged by size: 1917 1384 920 831 268 AfeI AGC'GCT Cuts at: 2585 2585 Size: 5320 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5123 AflIII A'CryG_T Cuts at: 1203 4219 5196 1203 Size: 3016 977 1327 Fragments arranged by size: 3016 1327 977 AgeI A'CCGG_T Cuts at: 4151 4151 Size: 5320 AhdI GACnn_n'nnGTC Cuts at: 4049 4049 Size: 5320 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5320 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5320 AlwNI CAG_nnn'CTG Cuts at: 2512 3063 4635 2512 Size: 551 1572 3197 Fragments arranged by size: 3197 1572 551 ApaLI G'TGCA_C Cuts at: 3377 4533 3377 Size: 1156 4164 ApoI r'AATT_y Cuts at: 1493 2629 2855 3007 5116 1493 Size: 1136 226 152 2109 1697 Fragments arranged by size: 2109 1697 1136 226 152 AseI AT'TA_AT Cuts at: 160 1032 3874 160 Size: 872 2842 1606 Fragments arranged by size: 2842 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5320 AvaI C'yCGr_G Cuts at: 1870 1943 2490 2645 1870 Size: 73 547 155 4545 Fragments arranged by size: 4545 547 155 73 BamHI G'GATC_C Cuts at: 2650 3017 2650 Size: 367 4953 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2011 2164 2641 4096 618 Size: 325 217 627 224 153 477 1455 1842 Fragments arranged by size: 1842 1455 627 477 325 224 217 153 BanII G_rGCy'C Cuts at: 1391 2639 1391 Size: 1248 4072 BbeI G_GCGC'C Cuts at: 2015 2168 2015 Size: 153 5167 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2451 928 Size: 971 552 3797 Fragments arranged by size: 3797 971 552 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3511 3545 1903 Size: 34 1574 34 3678 Fragments arranged by size: 3678 1574 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3212 4422 3212 Size: 1210 4110 BclI T'GATC_A Cuts at: 1548 1548 Size: 5320 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2144 3931 Size: 107 122 71 631 605 337 135 1787 Cuts at: 3931 136 Size: 1525 Fragments arranged by size: 1787 1525 631 605 337 135 122 107 71 BglII A'GATC_T Cuts at: 5315 5315 Size: 5320 BlpI GC'TnA_GC Cuts at: 2737 2737 Size: 5320 Bme1580I G_kGCm'C Cuts at: 1790 3381 4537 1790 Size: 1591 1156 2573 Fragments arranged by size: 2573 1591 1156 BmgBI CAC'GTC Cuts at: 1318 2241 1318 Size: 923 4397 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2318 2389 3051 Size: 416 53 83 399 24 901 71 662 Cuts at: 3051 4009 442 Size: 958 1753 Fragments arranged by size: 1753 958 901 662 416 399 83 71 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5320 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2215 2215 Size: 5320 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3980 1239 Size: 2741 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3445 4310 4608 3445 Size: 865 298 4157 Fragments arranged by size: 4157 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3983 882 Size: 617 2484 2219 Fragments arranged by size: 2484 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3111 493 Size: 711 1907 2702 Fragments arranged by size: 2702 1907 711 BsaBI GATnn'nnATC Cuts at: 5314 5314 Size: 5320 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2012 2153 2165 2196 Size: 53 83 186 936 479 141 12 31 Cuts at: 2196 3127 3509 4144 275 Size: 931 382 635 1451 Fragments arranged by size: 1451 936 931 635 479 382 186 141 83 53 31 12 BsaWI w'CCGG_w Cuts at: 3753 4151 4425 4572 4983 3753 Size: 398 274 147 411 4090 Fragments arranged by size: 4090 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5095 Fragments arranged by size: 5095 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2447 1186 Size: 1261 4059 BseYI C'CCAG_C Cuts at: 2322 2545 4523 5019 2322 Size: 223 1978 496 2623 Fragments arranged by size: 2623 1978 496 223 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2485 2507 1163 Size: 573 749 22 3976 Fragments arranged by size: 3976 749 573 22 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2100 2139 2213 2770 3531 4559 Size: 275 186 99 39 74 557 761 1028 Cuts at: 4559 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 557 275 186 99 74 39 BsiHKAI G_wGCw'C Cuts at: 3381 3466 4537 3381 Size: 85 1071 4164 Fragments arranged by size: 4164 1071 85 BsmI GAATG_Cn' Cuts at: 2831 2924 2831 Size: 93 5227 BsmBI CGTCTCn'nnnn_ Cuts at: 2124 2124 Size: 5320 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2639 3381 3466 4537 1391 Size: 399 849 742 85 1071 2174 Fragments arranged by size: 2174 1071 849 742 399 85 BspEI T'CCGG_A Cuts at: 4983 4983 Size: 5320 BspHI T'CATG_A Cuts at: 3209 4939 3209 Size: 1730 3590 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2370 2529 2675 844 Size: 1526 159 146 3489 Fragments arranged by size: 3489 1526 159 146 BsrBI CCG'CTC Cuts at: 1394 1823 2023 3207 1394 Size: 429 200 1184 3507 Fragments arranged by size: 3507 1184 429 200 BsrDI GCAATG_nn' Cuts at: 65 1248 3815 3989 65 Size: 1183 2567 174 1396 Fragments arranged by size: 2567 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3964 4151 1700 Size: 110 2154 187 2869 Fragments arranged by size: 2869 2154 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5320 BssHII G'CGCG_C Cuts at: 1925 2067 2069 1925 Size: 142 2 5176 Fragments arranged by size: 5176 142 2 BssSI C'ACGA_G Cuts at: 3380 4392 3380 Size: 1012 4308 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5320 BstBI TT'CG_AA Cuts at: 4124 4124 Size: 5320 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5320 BstYI r'GATC_y Cuts at: 1972 2650 3017 3402 3419 4871 4957 4969 5315 Size: 678 367 385 17 1452 86 12 346 Cuts at: 5315 1972 Size: 1977 Fragments arranged by size: 1977 1452 678 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2035 513 Size: 552 939 31 3798 Fragments arranged by size: 3798 939 552 31 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4581 Fragments arranged by size: 4581 683 56 BtsI GCAGTG_nn' Cuts at: 2836 3630 3650 2836 Size: 794 20 4506 Fragments arranged by size: 4506 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5320 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5285 DraI TTT'AAA Cuts at: 2624 2624 Size: 5320 DraIII CAC_nnn'GTG Cuts at: 2508 2508 Size: 5320 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4327 809 Size: 507 3011 1802 Fragments arranged by size: 3011 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2136 2210 2343 Size: 54 1425 50 275 18 306 74 133 Cuts at: 2343 2767 3656 5285 8 Size: 424 889 1629 43 Fragments arranged by size: 1629 1425 889 424 306 275 133 74 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2136 2210 2767 1537 Size: 275 324 74 557 4090 Fragments arranged by size: 4090 557 324 275 74 EarI CTCTTCn'nnn_ Cuts at: 1353 2054 3250 1353 Size: 701 1196 3423 Fragments arranged by size: 3423 1196 701 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2140 3907 4281 4427 387 Size: 1064 138 551 1767 374 146 1280 Fragments arranged by size: 1767 1280 1064 551 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2463 3383 3980 4767 5035 1239 Size: 393 831 920 597 787 268 1524 Fragments arranged by size: 1524 920 831 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2203 2203 Size: 5320 EcoRI G'AATT_C Cuts at: 2629 3007 2629 Size: 378 4942 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2575 2575 Size: 5320 FspI TGC'GCA Cuts at: 3826 3826 Size: 5320 HaeII r_GCGC'y Cuts at: 1678 2015 2168 2587 4467 1678 Size: 337 153 419 1880 2531 Fragments arranged by size: 2531 1880 419 337 153 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3961 3993 4035 4067 1373 Size: 32 135 32 2389 32 42 32 2626 Fragments arranged by size: 2626 2389 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2230 2664 2910 669 Size: 988 281 292 434 246 3079 Fragments arranged by size: 3079 988 434 292 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5320 HpaI GTT'AAC Cuts at: 2910 2910 Size: 5320 KasI G'GCGC_C Cuts at: 2011 2164 2011 Size: 153 5167 KpnI G_GTAC'C Cuts at: 2645 2645 Size: 5320 MfeI C'AATT_G Cuts at: 2919 2919 Size: 5320 MluI A'CGCG_T Cuts at: 5196 5196 Size: 5320 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4434 4618 1305 Size: 3129 184 2007 Fragments arranged by size: 3129 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3498 Fragments arranged by size: 3498 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3278 3637 3796 518 Size: 648 2112 359 159 2042 Fragments arranged by size: 2112 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2322 2736 3413 4561 4806 1606 Size: 716 414 677 1148 245 2120 Fragments arranged by size: 2120 1148 716 677 414 245 NarI GG'CG_CC Cuts at: 2012 2165 2012 Size: 153 5167 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3829 Fragments arranged by size: 3829 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5320 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5320 NotI GC'GGCC_GC Cuts at: 2136 2767 2136 Size: 631 4689 NruI TCG'CGA Cuts at: 2301 2301 Size: 5320 NspI r_CATG'y Cuts at: 1132 1229 2499 2678 4223 1132 Size: 97 1270 179 1545 2229 Fragments arranged by size: 2229 1545 1270 179 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5320 PciI A'CATG_T Cuts at: 4219 4219 Size: 5320 PflMI CCAn_nnn'nTGG Cuts at: 1108 2341 1108 Size: 1233 4087 PfoI T'CCnGG_A Cuts at: 2201 2201 Size: 5320 PmeI GTTT'AAAC Cuts at: 2624 2624 Size: 5320 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3389 3421 4902 4934 3389 Size: 32 1481 32 3775 Fragments arranged by size: 3775 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5320 PsiI TTA'TAA Cuts at: 2890 2890 Size: 5320 PstI C_TGCA'G Cuts at: 830 2672 830 Size: 1842 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5320 PvuII CAG'CTG Cuts at: 2322 2322 Size: 5320 SalI G'TCGA_C Cuts at: 1936 2228 2662 1936 Size: 292 434 4594 Fragments arranged by size: 4594 434 292 SbfI CC_TGCA'GG Cuts at: 2672 2672 Size: 5320 ScaI AGT'ACT Cuts at: 3568 5280 3568 Size: 1712 3608 SfcI C'TryA_G Cuts at: 826 1045 2668 3803 4484 4675 826 Size: 219 1623 1135 681 191 1471 Fragments arranged by size: 1623 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2144 2144 Size: 5320 SfoI GGC'GCC Cuts at: 2013 2166 2013 Size: 153 5167 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5320 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5320 SmaI CCC'GGG Cuts at: 1872 2492 2647 1872 Size: 620 155 4545 Fragments arranged by size: 4545 620 155 SmlI C'TyrA_G Cuts at: 820 1017 1943 3424 4325 4587 820 Size: 197 926 1481 901 262 1553 Fragments arranged by size: 1553 1481 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3111 493 Size: 2618 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5320 SphI G_CATG'C Cuts at: 1132 2499 2678 1132 Size: 1367 179 3774 Fragments arranged by size: 3774 1367 179 SspI AAT'ATT Cuts at: 5 52 3244 5 Size: 47 3192 2081 Fragments arranged by size: 3192 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5320 StyI C'CwwG_G Cuts at: 513 1065 2004 2759 513 Size: 552 939 755 3074 Fragments arranged by size: 3074 939 755 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2114 2490 3347 3364 3517 3702 4147 Size: 1255 534 376 857 17 153 185 445 Cuts at: 4147 325 Size: 1498 Fragments arranged by size: 1498 1255 857 534 445 376 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3566 5278 96 Size: 274 80 33 51 1443 1589 1712 138 Fragments arranged by size: 1712 1589 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5280 5312 5280 Size: 32 5288 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5320 XbaI T'CTAG_A Cuts at: 2656 2656 Size: 5320 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5320 XmaI C'CCGG_G Cuts at: 1870 2490 2645 1870 Size: 620 155 4545 Fragments arranged by size: 4545 620 155 XmnI GAAnn'nnTTC Cuts at: 2048 3449 2048 Size: 1401 3919 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3128 276 Size: 53 83 186 936 1594 2468 Fragments arranged by size: 2468 1594 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoO109I EcoRV FseI FspAI NaeI NgoMIV NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SrfI SwaI XcmI