(Circular) (Six-base) MAPSORT of: pFN21AB8020.seq Check: 56 from: 1 to: 6775 pFN21AB8020 6775 bp With 182 enzymes: SgfI * August 20, 2012 11:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4585 278 Size: 53 83 186 936 3049 2468 Fragments arranged by size: 3049 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4118 1937 Size: 2181 4594 Acc65I G'GTAC_C Cuts at: 2519 4096 2519 Size: 1577 5198 AclI AA'CG_TT Cuts at: 1563 2380 4902 5275 1563 Size: 817 2522 373 3063 Fragments arranged by size: 3063 2522 817 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2340 2840 3209 4838 6222 6490 1632 Size: 708 500 369 1629 1384 268 1917 Fragments arranged by size: 1917 1629 1384 708 500 369 268 AfeI AGC'GCT Cuts at: 2647 2647 Size: 6775 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6578 AflIII A'CryG_T Cuts at: 1203 5674 6651 1203 Size: 4471 977 1327 Fragments arranged by size: 4471 1327 977 AgeI A'CCGG_T Cuts at: 5606 5606 Size: 6775 AhdI GACnn_n'nnGTC Cuts at: 5504 5504 Size: 6775 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6775 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2948 1184 Size: 1764 5011 AlwNI CAG_nnn'CTG Cuts at: 2564 4518 6090 2564 Size: 1954 1572 3249 Fragments arranged by size: 3249 1954 1572 ApaI G_GGCC'C Cuts at: 2499 2499 Size: 6775 ApaLI G'TGCA_C Cuts at: 4832 5988 4832 Size: 1156 5619 ApoI r'AATT_y Cuts at: 1493 2709 3247 4084 4310 4462 6571 1493 Size: 1216 538 837 226 152 2109 1697 Fragments arranged by size: 2109 1697 1216 837 538 226 152 AseI AT'TA_AT Cuts at: 160 1032 5329 160 Size: 872 4297 1606 Fragments arranged by size: 4297 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6775 AvaI C'yCGr_G Cuts at: 1870 1943 2901 4100 1870 Size: 73 958 1199 4545 Fragments arranged by size: 4545 1199 958 73 BamHI G'GATC_C Cuts at: 4105 4472 4105 Size: 367 6408 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2519 4096 5551 618 Size: 325 217 627 732 1577 1455 1842 Fragments arranged by size: 1842 1577 1455 732 627 325 217 BanII G_rGCy'C Cuts at: 1391 2499 4094 1391 Size: 1108 1595 4072 Fragments arranged by size: 4072 1595 1108 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5804 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4966 5000 1903 Size: 34 3029 34 3678 Fragments arranged by size: 3678 3029 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4667 5877 4667 Size: 1210 5565 BclI T'GATC_A Cuts at: 1548 1548 Size: 6775 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5386 136 Size: 107 122 71 631 605 3714 1525 Fragments arranged by size: 3714 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6770 6770 Size: 6775 BlpI GC'TnA_GC Cuts at: 3146 4192 3146 Size: 1046 5729 Bme1580I G_kGCm'C Cuts at: 1790 2499 3183 4836 5992 1790 Size: 709 684 1653 1156 2573 Fragments arranged by size: 2573 1653 1156 709 684 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6775 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4506 5464 442 Size: 416 53 83 399 24 3089 958 1753 Fragments arranged by size: 3089 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6775 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2411 2439 2465 2514 2670 2862 5435 1239 Size: 1172 28 26 49 156 192 2573 2579 Fragments arranged by size: 2579 2573 1172 192 156 49 28 26 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2454 3683 4900 5765 6063 2454 Size: 1229 1217 865 298 3166 Fragments arranged by size: 3166 1229 1217 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2838 5438 882 Size: 617 1339 2600 2219 Fragments arranged by size: 2600 2219 1339 617 BsaAI yAC'GTr Cuts at: 493 1204 4566 493 Size: 711 3362 2702 Fragments arranged by size: 3362 2702 711 BsaBI GATnn'nnATC Cuts at: 6769 6769 Size: 6775 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4582 4964 5599 275 Size: 53 83 186 936 3049 382 635 1451 Fragments arranged by size: 3049 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2265 3202 5208 5606 5880 6027 6438 2265 Size: 937 2006 398 274 147 411 2602 Fragments arranged by size: 2602 2006 937 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2512 2542 3228 3258 1178 Size: 30 165 30 1109 30 686 30 4695 Fragments arranged by size: 4695 1109 686 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2038 2041 2624 2654 3695 3716 1186 Size: 852 3 583 30 1041 21 4245 Fragments arranged by size: 4245 1041 852 583 30 21 3 BseYI C'CCAG_C Cuts at: 2916 3276 3455 3779 5978 6474 2916 Size: 360 179 324 2199 496 3217 Fragments arranged by size: 3217 2199 496 360 324 179 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3984 1163 Size: 573 2248 3954 Fragments arranged by size: 3954 2248 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4225 4986 6014 1540 Size: 275 186 2224 761 1028 2301 Fragments arranged by size: 2301 2224 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2977 4836 4921 5992 2977 Size: 1859 85 1071 3760 Fragments arranged by size: 3760 1859 1071 85 BsmI GAATG_Cn' Cuts at: 4286 4379 4286 Size: 93 6682 BsmBI CGTCTCn'nnnn_ Cuts at: 2595 2817 2595 Size: 222 6553 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2499 2977 3183 4094 4836 4921 5992 Size: 399 709 478 206 911 742 85 1071 Cuts at: 5992 1391 Size: 2174 Fragments arranged by size: 2174 1071 911 742 709 478 399 206 85 BspEI T'CCGG_A Cuts at: 3202 6438 3202 Size: 3236 3539 BspHI T'CATG_A Cuts at: 4664 6394 4664 Size: 1730 5045 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2507 4130 844 Size: 1663 1623 3489 Fragments arranged by size: 3489 1663 1623 BsrBI CCG'CTC Cuts at: 1394 1823 2572 4662 1394 Size: 429 749 2090 3507 Fragments arranged by size: 3507 2090 749 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2814 2887 3160 3621 5270 5444 65 Size: 1183 1566 73 273 461 1649 174 1396 Fragments arranged by size: 1649 1566 1396 1183 461 273 174 73 BsrFI r'CCGG_y Cuts at: 1700 1810 5419 5606 1700 Size: 110 3609 187 2869 Fragments arranged by size: 3609 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2115 96 Size: 2019 4756 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6775 BssSI C'ACGA_G Cuts at: 3003 3528 4835 5847 3003 Size: 525 1307 1012 3931 Fragments arranged by size: 3931 1307 1012 525 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6775 BstBI TT'CG_AA Cuts at: 5579 5579 Size: 6775 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6775 BstYI r'GATC_y Cuts at: 1972 2509 3373 4105 4472 4857 4874 6326 6412 Size: 537 864 732 367 385 17 1452 86 Cuts at: 6412 6424 6770 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 864 732 537 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3514 3981 513 Size: 552 939 1510 467 3307 Fragments arranged by size: 3307 1510 939 552 467 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6036 Fragments arranged by size: 6036 683 56 BtsI GCAGTG_nn' Cuts at: 2565 2930 4291 5085 5105 2565 Size: 365 1361 794 20 4235 Fragments arranged by size: 4235 1361 794 365 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6775 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6740 DraI TTT'AAA Cuts at: 4079 4079 Size: 6775 DraIII CAC_nnn'GTG Cuts at: 2198 2198 Size: 6775 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2587 5782 809 Size: 507 1271 3195 1802 Fragments arranged by size: 3195 1802 1271 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2083 3168 3960 Size: 54 1425 50 275 18 253 1085 792 Cuts at: 3960 4222 5111 6740 8 Size: 262 889 1629 43 Fragments arranged by size: 1629 1425 1085 889 792 275 262 253 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4222 1537 Size: 275 2410 4090 Fragments arranged by size: 4090 2410 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2059 2598 3003 3033 3858 4705 1353 Size: 706 539 405 30 825 847 3423 Fragments arranged by size: 3423 847 825 706 539 405 30 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5362 5736 5882 387 Size: 1064 138 3773 374 146 1280 Fragments arranged by size: 3773 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2340 2411 2439 2465 2514 2670 2840 Size: 393 708 71 28 26 49 156 170 Cuts at: 2840 2862 3209 4838 5435 6222 6490 1239 Size: 22 347 1629 597 787 268 1524 Fragments arranged by size: 1629 1524 787 708 597 393 347 268 170 156 71 49 28 26 22 EcoRI G'AATT_C Cuts at: 4084 4462 4084 Size: 378 6397 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3044 3044 Size: 6775 FspI TGC'GCA Cuts at: 5281 5281 Size: 6775 HaeII r_GCGC'y Cuts at: 1678 2649 2962 5922 1678 Size: 971 313 2960 2531 Fragments arranged by size: 2960 2531 971 313 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2015 2047 2981 3013 5416 Size: 32 135 32 443 32 934 32 2403 Cuts at: 5416 5448 5490 5522 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2403 934 443 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2863 3978 4119 4365 669 Size: 988 281 925 1115 141 246 3079 Fragments arranged by size: 3079 1115 988 925 281 246 141 HindIII A'AGCT_T Cuts at: 748 748 Size: 6775 HpaI GTT'AAC Cuts at: 4365 4365 Size: 6775 KpnI G_GTAC'C Cuts at: 2523 4100 2523 Size: 1577 5198 MfeI C'AATT_G Cuts at: 3255 4374 3255 Size: 1119 5656 MluI A'CGCG_T Cuts at: 6651 6651 Size: 6775 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2241 3093 5889 6073 1305 Size: 936 852 2796 184 2007 Fragments arranged by size: 2796 2007 936 852 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2085 3170 10 Size: 54 1425 343 253 1085 3615 Fragments arranged by size: 3615 1425 1085 343 253 54 MslI CAynn'nnrTG Cuts at: 518 1166 2895 3494 3956 4733 5092 5251 518 Size: 648 1729 599 462 777 359 159 2042 Fragments arranged by size: 2042 1729 777 648 599 462 359 159 MspA1I CmG'CkG Cuts at: 1606 4191 4868 6016 6261 1606 Size: 2585 677 1148 245 2120 Fragments arranged by size: 2585 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3514 513 Size: 552 939 1510 3774 Fragments arranged by size: 3774 1510 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6775 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6775 NotI GC'GGCC_GC Cuts at: 4222 4222 Size: 6775 NspI r_CATG'y Cuts at: 1132 1229 3396 4133 5678 1132 Size: 97 2167 737 1545 2229 Fragments arranged by size: 2229 2167 1545 737 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6775 PciI A'CATG_T Cuts at: 5674 5674 Size: 6775 PflMI CCAn_nnn'nTGG Cuts at: 1108 3515 1108 Size: 2407 4368 PfoI T'CCnGG_A Cuts at: 2416 2790 2416 Size: 374 6401 PmeI GTTT'AAAC Cuts at: 4079 4079 Size: 6775 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4844 4876 6357 6389 4844 Size: 32 1481 32 5230 Fragments arranged by size: 5230 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6775 PsiI TTA'TAA Cuts at: 4345 4345 Size: 6775 PspOMI G'GGCC_C Cuts at: 2495 2495 Size: 6775 PstI C_TGCA'G Cuts at: 830 2181 2518 4127 830 Size: 1351 337 1609 3478 Fragments arranged by size: 3478 1609 1351 337 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6775 SalI G'TCGA_C Cuts at: 1936 4117 1936 Size: 2181 4594 SapI GCTCTTCn'nnn_ Cuts at: 3033 3033 Size: 6775 SbfI CC_TGCA'GG Cuts at: 2518 4127 2518 Size: 1609 5166 ScaI AGT'ACT Cuts at: 3715 5023 6735 3715 Size: 1308 1712 3755 Fragments arranged by size: 3755 1712 1308 SfcI C'TryA_G Cuts at: 826 1045 2177 2514 4123 5258 5939 6130 826 Size: 219 1132 337 1609 1135 681 191 1471 Fragments arranged by size: 1609 1471 1135 1132 681 337 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6775 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6775 SmaI CCC'GGG Cuts at: 1872 4102 1872 Size: 2230 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2469 3662 4879 5780 6042 820 Size: 197 926 526 1193 1217 901 262 1553 Fragments arranged by size: 1553 1217 1193 926 901 526 262 197 SnaBI TAC'GTA Cuts at: 493 4566 493 Size: 4073 2702 SpeI A'CTAG_T Cuts at: 152 2459 4003 152 Size: 2307 1544 2924 Fragments arranged by size: 2924 2307 1544 SphI G_CATG'C Cuts at: 1132 4133 1132 Size: 3001 3774 SspI AAT'ATT Cuts at: 5 52 4699 5 Size: 47 4647 2081 Fragments arranged by size: 4647 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6775 StyI C'CwwG_G Cuts at: 513 1065 2004 3439 3514 4214 513 Size: 552 939 1435 75 700 3074 Fragments arranged by size: 3074 1435 939 700 552 75 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2706 2744 3174 4802 4819 4972 5157 Size: 1255 1126 38 430 1628 17 153 185 Cuts at: 5157 5602 325 Size: 445 1498 Fragments arranged by size: 1628 1498 1255 1126 445 430 185 153 38 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2115 3713 5021 Size: 274 80 33 51 1443 138 1598 1308 Cuts at: 5021 6733 96 Size: 1712 138 Fragments arranged by size: 1712 1598 1443 1308 274 138 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3228 3260 3651 3683 6735 6767 3228 Size: 32 391 32 3052 32 3236 Fragments arranged by size: 3236 3052 391 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2147 1316 Size: 831 5944 XbaI T'CTAG_A Cuts at: 4111 4111 Size: 6775 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6775 XmaI C'CCGG_G Cuts at: 1870 4100 1870 Size: 2230 4545 XmnI GAAnn'nnTTC Cuts at: 2249 4904 2249 Size: 2655 4120 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4583 276 Size: 53 83 186 936 3049 2468 Fragments arranged by size: 3049 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI