(Circular) (Six-base) MAPSORT of: pFN21AE0850.seq Check: 209 from: 1 to: 5656 pFN21AE0850 5656 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3466 278 Size: 53 83 186 936 1930 2468 Fragments arranged by size: 2468 1930 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2999 1937 Size: 1062 4594 Acc65I G'GTAC_C Cuts at: 2977 2977 Size: 5656 AclI AA'CG_TT Cuts at: 1563 3783 4156 1563 Size: 2220 373 3063 Fragments arranged by size: 3063 2220 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2751 3719 5103 5371 1632 Size: 1119 968 1384 268 1917 Fragments arranged by size: 1917 1384 1119 968 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5459 AflIII A'CryG_T Cuts at: 1203 4555 5532 1203 Size: 3352 977 1327 Fragments arranged by size: 3352 1327 977 AgeI A'CCGG_T Cuts at: 4487 4487 Size: 5656 AhdI GACnn_n'nnGTC Cuts at: 4385 4385 Size: 5656 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5656 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5656 AlwNI CAG_nnn'CTG Cuts at: 3399 4971 3399 Size: 1572 4084 ApaLI G'TGCA_C Cuts at: 2663 3713 4869 2663 Size: 1050 1156 3450 Fragments arranged by size: 3450 1156 1050 ApoI r'AATT_y Cuts at: 1493 2033 2965 3191 3343 5452 1493 Size: 540 932 226 152 2109 1697 Fragments arranged by size: 2109 1697 932 540 226 152 AseI AT'TA_AT Cuts at: 160 1032 4210 160 Size: 872 3178 1606 Fragments arranged by size: 3178 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5656 AvaI C'yCGr_G Cuts at: 1870 1943 2483 2981 1870 Size: 73 540 498 4545 Fragments arranged by size: 4545 540 498 73 BamHI G'GATC_C Cuts at: 2986 3353 2986 Size: 367 5289 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2977 4432 618 Size: 325 217 627 1190 1455 1842 Fragments arranged by size: 1842 1455 1190 627 325 217 BanII G_rGCy'C Cuts at: 1391 2063 2508 2975 1391 Size: 672 445 467 4072 Fragments arranged by size: 4072 672 467 445 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2311 2720 928 Size: 971 412 409 3864 Fragments arranged by size: 3864 971 412 409 BbvCI CC'TCA_GC Cuts at: 2684 2684 Size: 5656 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2464 2498 3847 3881 1903 Size: 34 50 34 443 34 1349 34 3678 Fragments arranged by size: 3678 1349 443 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3548 4758 3548 Size: 1210 4446 BclI T'GATC_A Cuts at: 1548 2466 1548 Size: 918 4738 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4267 136 Size: 107 122 71 631 605 2595 1525 Fragments arranged by size: 2595 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5651 5651 Size: 5656 BlpI GC'TnA_GC Cuts at: 3073 3073 Size: 5656 Bme1580I G_kGCm'C Cuts at: 1790 2667 2920 3717 4873 1790 Size: 877 253 797 1156 2573 Fragments arranged by size: 2573 1156 877 797 253 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5656 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3387 4345 442 Size: 416 53 83 399 24 1970 958 1753 Fragments arranged by size: 1970 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5656 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2099 2099 Size: 5656 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4316 1239 Size: 3077 2579 Bpu10I CC'TnA_GC Cuts at: 2009 2684 2009 Size: 675 4981 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3781 4646 4944 3781 Size: 865 298 4493 Fragments arranged by size: 4493 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4319 882 Size: 617 2820 2219 Fragments arranged by size: 2820 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3447 493 Size: 711 2243 2702 Fragments arranged by size: 2702 2243 711 BsaBI GATnn'nnATC Cuts at: 2252 5650 2252 Size: 3398 2258 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3463 3845 4480 275 Size: 53 83 186 936 1930 382 635 1451 Fragments arranged by size: 1930 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4089 4487 4761 4908 5319 4089 Size: 398 274 147 411 4426 Fragments arranged by size: 4426 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2267 2297 1178 Size: 30 165 30 864 30 4537 Fragments arranged by size: 4537 864 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2686 2689 1186 Size: 1500 3 4153 Fragments arranged by size: 4153 1500 3 BseYI C'CCAG_C Cuts at: 2940 4859 5355 2940 Size: 1919 496 3241 Fragments arranged by size: 3241 1919 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5083 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3106 3867 4895 1540 Size: 275 186 1105 761 1028 2301 Fragments arranged by size: 2301 1105 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2063 2646 2667 3717 3802 4873 2063 Size: 583 21 1050 85 1071 2846 Fragments arranged by size: 2846 1071 1050 583 85 21 BsmI GAATG_Cn' Cuts at: 3167 3260 3167 Size: 93 5563 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2063 2508 2646 2667 2920 2975 3717 Size: 399 273 445 138 21 253 55 742 Cuts at: 3717 3802 4873 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 445 399 273 253 138 85 55 21 BspEI T'CCGG_A Cuts at: 5319 5319 Size: 5656 BspHI T'CATG_A Cuts at: 3545 5275 3545 Size: 1730 3926 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3011 844 Size: 2167 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3543 1394 Size: 429 1720 3507 Fragments arranged by size: 3507 1720 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2078 4151 4325 65 Size: 1183 830 2073 174 1396 Fragments arranged by size: 2073 1396 1183 830 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4300 4487 1700 Size: 110 2490 187 2869 Fragments arranged by size: 2869 2490 187 110 BsrGI T'GTAC_A Cuts at: 96 2838 96 Size: 2742 2914 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5656 BssSI C'ACGA_G Cuts at: 2042 2073 2261 3716 4728 2042 Size: 31 188 1455 1012 2970 Fragments arranged by size: 2970 1455 1012 188 31 BstAPI GCAn_nnn'nTGC Cuts at: 794 2606 794 Size: 1812 3844 BstBI TT'CG_AA Cuts at: 4460 4460 Size: 5656 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5656 BstYI r'GATC_y Cuts at: 1972 2986 3353 3738 3755 5207 5293 5305 5651 Size: 1014 367 385 17 1452 86 12 346 Cuts at: 5651 1972 Size: 1977 Fragments arranged by size: 1977 1452 1014 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2701 2701 Size: 5656 BtgI C'CryG_G Cuts at: 513 1065 2118 2637 2907 513 Size: 552 1053 519 270 3262 Fragments arranged by size: 3262 1053 552 519 270 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4917 Fragments arranged by size: 4917 683 56 BtsI GCAGTG_nn' Cuts at: 3172 3966 3986 3172 Size: 794 20 4842 Fragments arranged by size: 4842 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5656 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2232 2267 529 Size: 35 1668 35 3918 Fragments arranged by size: 3918 1668 35 35 DraI TTT'AAA Cuts at: 2960 2960 Size: 5656 DraIII CAC_nnn'GTG Cuts at: 2263 2263 Size: 5656 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2279 4663 809 Size: 507 963 2384 1802 Fragments arranged by size: 2384 1802 963 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2121 2197 2334 Size: 54 1425 50 275 18 291 76 137 Cuts at: 2334 2673 3103 3992 5621 8 Size: 339 430 889 1629 43 Fragments arranged by size: 1629 1425 889 430 339 291 275 137 76 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3103 1537 Size: 275 1291 4090 Fragments arranged by size: 4090 1291 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2099 3586 1353 Size: 746 1487 3423 Fragments arranged by size: 3423 1487 746 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4243 4617 4763 387 Size: 1064 138 2654 374 146 1280 Fragments arranged by size: 2654 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2061 2061 Size: 5656 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2751 3719 4316 5103 5371 1239 Size: 393 1119 968 597 787 268 1524 Fragments arranged by size: 1524 1119 968 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2548 2578 2548 Size: 30 5626 EcoRI G'AATT_C Cuts at: 2965 3343 2965 Size: 378 5278 FspI TGC'GCA Cuts at: 4162 4162 Size: 5656 HaeII r_GCGC'y Cuts at: 1678 4803 1678 Size: 3125 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4297 4329 4371 4403 1373 Size: 32 135 32 2725 32 42 32 2626 Fragments arranged by size: 2725 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3000 3246 669 Size: 988 281 1062 246 3079 Fragments arranged by size: 3079 1062 988 281 246 HindIII A'AGCT_T Cuts at: 748 2533 748 Size: 1785 3871 HpaI GTT'AAC Cuts at: 3246 3246 Size: 5656 KpnI G_GTAC'C Cuts at: 2981 2981 Size: 5656 MfeI C'AATT_G Cuts at: 3255 3255 Size: 5656 MluI A'CGCG_T Cuts at: 5532 5532 Size: 5656 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4770 4954 1305 Size: 3465 184 2007 Fragments arranged by size: 3465 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2123 2675 10 Size: 54 1425 343 291 552 2991 Fragments arranged by size: 2991 1425 552 343 291 54 MslI CAynn'nnrTG Cuts at: 518 1166 2123 3614 3973 4132 518 Size: 648 957 1491 359 159 2042 Fragments arranged by size: 2042 1491 957 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3072 3749 4897 5142 1606 Size: 1466 677 1148 245 2120 Fragments arranged by size: 2120 1466 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2118 2637 2907 513 Size: 552 1053 519 270 3262 Fragments arranged by size: 3262 1053 552 519 270 NdeI CA'TA_TG Cuts at: 387 387 Size: 5656 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5656 NotI GC'GGCC_GC Cuts at: 3103 3103 Size: 5656 NspI r_CATG'y Cuts at: 1132 1229 3014 4559 1132 Size: 97 1785 1545 2229 Fragments arranged by size: 2229 1785 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5656 PciI A'CATG_T Cuts at: 4555 4555 Size: 5656 PflMI CCAn_nnn'nTGG Cuts at: 1108 2263 1108 Size: 1155 4501 PfoI T'CCnGG_A Cuts at: 2153 2211 2153 Size: 58 5598 PmeI GTTT'AAAC Cuts at: 2960 2960 Size: 5656 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3725 3757 5238 5270 3725 Size: 32 1481 32 4111 Fragments arranged by size: 4111 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2578 2578 Size: 5656 PshAI GACnn'nnGTC Cuts at: 1613 2747 1613 Size: 1134 4522 PsiI TTA'TAA Cuts at: 3226 3226 Size: 5656 PstI C_TGCA'G Cuts at: 830 3008 830 Size: 2178 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5656 SacI G_AGCT'C Cuts at: 2063 2063 Size: 5656 SalI G'TCGA_C Cuts at: 1936 2998 1936 Size: 1062 4594 SanDI GG'GwC_CC Cuts at: 2578 2578 Size: 5656 SbfI CC_TGCA'GG Cuts at: 3008 3008 Size: 5656 ScaI AGT'ACT Cuts at: 3904 5616 3904 Size: 1712 3944 SfcI C'TryA_G Cuts at: 826 1045 2107 3004 4139 4820 5011 826 Size: 219 1062 897 1135 681 191 1471 Fragments arranged by size: 1471 1135 1062 897 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5656 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5656 SmaI CCC'GGG Cuts at: 1872 2983 1872 Size: 1111 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2483 3760 4661 4923 820 Size: 197 926 540 1277 901 262 1553 Fragments arranged by size: 1553 1277 926 901 540 262 197 SnaBI TAC'GTA Cuts at: 493 3447 493 Size: 2954 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5656 SphI G_CATG'C Cuts at: 1132 3014 1132 Size: 1882 3774 SspI AAT'ATT Cuts at: 5 52 3580 5 Size: 47 3528 2081 Fragments arranged by size: 3528 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5656 StyI C'CwwG_G Cuts at: 513 1065 2118 2637 2907 3095 513 Size: 552 1053 519 270 188 3074 Fragments arranged by size: 3074 1053 552 519 270 188 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3683 3700 3853 4038 4483 325 Size: 1255 2103 17 153 185 445 1498 Fragments arranged by size: 2103 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2184 2310 2838 Size: 274 80 33 51 1443 207 126 528 Cuts at: 2838 3902 5614 96 Size: 1064 1712 138 Fragments arranged by size: 1712 1443 1064 528 274 207 138 126 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2267 2299 5616 5648 2267 Size: 32 3317 32 2275 Fragments arranged by size: 3317 2275 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5656 XbaI T'CTAG_A Cuts at: 2992 2992 Size: 5656 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2801 2801 Size: 5656 XhoI C'TCGA_G Cuts at: 1943 2483 1943 Size: 540 5116 XmaI C'CCGG_G Cuts at: 1870 2981 1870 Size: 1111 4545 XmnI GAAnn'nnTTC Cuts at: 2717 3785 2717 Size: 1068 4588 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3464 276 Size: 53 83 186 936 1930 2468 Fragments arranged by size: 2468 1930 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI