Restriction map for
pFN21AE0850
(Linear) (Six-base) MAPSORT of: KIEE0850.seq Check: 398 from: 1 to: 951
KIEE0850.orf
With 182 enzymes: SgfI *
March 2, 2010 14:31 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 746 951
Size: 746 205
ApaLI G'TGCA_C
Cuts at: 0 658 951
Size: 658 293
ApoI r'AATT_y
Cuts at: 0 28 951
Size: 28 923
AvaI C'yCGr_G
Cuts at: 0 478 951
Size: 478 473
BanII G_rGCy'C
Cuts at: 0 58 503 951
Size: 58 445 448
Fragments arranged by size:
448 445 58
BbsI GAAGACnn'nnnn_
Cuts at: 0 306 715 951
Size: 306 409 236
Fragments arranged by size:
409 306 236
BbvCI CC'TCA_GC
Cuts at: 0 679 951
Size: 679 272
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 459 493 951
Size: 459 34 458
Fragments arranged by size:
459 458 34
BclI T'GATC_A
Cuts at: 0 461 951
Size: 461 490
Bme1580I G_kGCm'C
Cuts at: 0 662 915 951
Size: 662 253 36
Fragments arranged by size:
662 253 36
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 94 951
Size: 94 857
Bpu10I CC'TnA_GC
Cuts at: 0 4 679 951
Size: 4 675 272
Fragments arranged by size:
675 272 4
BsaBI GATnn'nnATC
Cuts at: 0 247 951
Size: 247 704
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 262 292 951
Size: 262 30 659
Fragments arranged by size:
659 262 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 681 684 951
Size: 681 3 267
Fragments arranged by size:
681 267 3
BseYI C'CCAG_C
Cuts at: 0 935 951
Size: 935 16
BsiHKAI G_wGCw'C
Cuts at: 0 58 641 662 951
Size: 58 583 21 289
Fragments arranged by size:
583 289 58 21
Bsp1286I G_dGCh'C
Cuts at: 0 58 503 641 662 915 951
Size: 58 445 138 21 253 36
Fragments arranged by size:
445 253 138 58 36 21
BsrDI GCAATG_nn'
Cuts at: 0 73 951
Size: 73 878
BsrGI T'GTAC_A
Cuts at: 0 833 951
Size: 833 118
BssSI C'ACGA_G
Cuts at: 0 37 68 256 951
Size: 37 31 188 695
Fragments arranged by size:
695 188 37 31
BstAPI GCAn_nnn'nTGC
Cuts at: 0 601 951
Size: 601 350
Bsu36I CC'TnA_GG
Cuts at: 0 696 951
Size: 696 255
BtgI C'CryG_G
Cuts at: 0 113 632 902 951
Size: 113 519 270 49
Fragments arranged by size:
519 270 113 49
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 227 262 951
Size: 227 35 689
Fragments arranged by size:
689 227 35
DraIII CAC_nnn'GTG
Cuts at: 0 258 951
Size: 258 693
DrdI GACnn_nn'nnGTC
Cuts at: 0 274 951
Size: 274 677
EaeI y'GGCC_r
Cuts at: 0 116 192 329 668 951
Size: 116 76 137 339 283
Fragments arranged by size:
339 283 137 116 76
EarI CTCTTCn'nnn_
Cuts at: 0 94 951
Size: 94 857
EcoICRI GAG'CTC
Cuts at: 0 56 951
Size: 56 895
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 746 951
Size: 746 205
EcoO109I rG'GnC_Cy
Cuts at: 0 543 573 951
Size: 543 30 378
Fragments arranged by size:
543 378 30
HindIII A'AGCT_T
Cuts at: 0 528 951
Size: 528 423
MscI TGG'CCA
Cuts at: 0 118 670 951
Size: 118 552 281
Fragments arranged by size:
552 281 118
MslI CAynn'nnrTG
Cuts at: 0 118 951
Size: 118 833
NcoI C'CATG_G
Cuts at: 0 113 632 902 951
Size: 113 519 270 49
Fragments arranged by size:
519 270 113 49
PflMI CCAn_nnn'nTGG
Cuts at: 0 258 951
Size: 258 693
PfoI T'CCnGG_A
Cuts at: 0 148 206 951
Size: 148 58 745
Fragments arranged by size:
745 148 58
PpuMI rG'GwC_Cy
Cuts at: 0 573 951
Size: 573 378
PshAI GACnn'nnGTC
Cuts at: 0 742 951
Size: 742 209
SacI G_AGCT'C
Cuts at: 0 58 951
Size: 58 893
SanDI GG'GwC_CC
Cuts at: 0 573 951
Size: 573 378
SfcI C'TryA_G
Cuts at: 0 102 951
Size: 102 849
SmlI C'TyrA_G
Cuts at: 0 478 951
Size: 478 473
StyI C'CwwG_G
Cuts at: 0 113 632 902 951
Size: 113 519 270 49
Fragments arranged by size:
519 270 113 49
TatI w'GTAC_w
Cuts at: 0 179 305 833 951
Size: 179 126 528 118
Fragments arranged by size:
528 179 126 118
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 262 294 951
Size: 262 32 657
Fragments arranged by size:
657 262 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 796 951
Size: 796 155
XhoI C'TCGA_G
Cuts at: 0 478 951
Size: 478 473
XmnI GAAnn'nnTTC
Cuts at: 0 712 951
Size: 712 239
Enzymes that do cut:
AcuI ApaLI ApoI AvaI BanII BbsI BbvCI BcgI
BclI Bme1580I BplI Bpu10I BsaBI BsaXI BseRI BseYI
BsiHKAI Bsp1286I BsrDI BsrGI BssSI BstAPI Bsu36I BtgI
CspCI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoO109I
HindIII MscI MslI NcoI PflMI PfoI PpuMI PshAI
SacI SanDI SfcI SmlI StyI TatI TstI XcmI
XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BciVI
BfrBI BglI BglII BlpI BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaHI BsaWI BsgI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrBI BsrFI BssHII
BstBI BstEII BstXI BstYI BstZ17I BtgZI BtsI ClaI
DraI EagI EciI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HaeII Hin4I HincII HpaI KasI KpnI
MfeI MluI MmeI MspA1I NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII Tth111I XbaI
XmaI ZraI