Restriction map for
pFN21AB6884
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# NO CUT ENZYMES ( 156 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BamHI
BanII BbeI BbrPI BcgI BciVI
BclI BcnI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsmFI
BsmI Bsp1407I BspDI BspEI BspHI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstF5I BstPI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT22I FauI
FbaI FokI FseI FspI HgaI
Hin1I HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MaeII MamI
MfeI MluI MroI MseI MunI
NarI NciI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I Tth111I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccII 34 CGCG CG^CG
AcsI 484 GAATTC [GA]^AATT[CT]
AfaI 207 GTAC GT^AC
AflIII 329 ACATGT A^C[GA][CT]GT
AlwNI 286 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 484 GAATTC [GA]^AATT[CT]
AvaI 238 CCCGAG C^[CT]CG[GA]G
BalI 2 TGGCCA TGG^CCA
BanI 234 GGTGCC G^G[CT][GA]CC
BbsI 259 GTCTTC GAAGAC(2/6)
BbvCI 301 GCTGAGG CCTCAGC(-5/-2)
BglI 553 GCCTCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 76 CTGGAG CTGGAG(16/14)
BpuAI 259 GTCTTC GAAGAC(2/6)
BsoBI 238 CCCGAG C^[CT]CG[GA]G
BspLU11I 329 ACATGT A^CATGT
BspMI 276 GCAGGT ACCTGC(4/8)
BsrBI 82 GAGCGG CCGCTC(-3/-3)
BsrI 449 ACTGG ACTGG(1/-1)
BstUI 34 CGCG CG^CG
BstYI 441 AGATCC [GA]^GATC[CT]
DrdI 307 GACTATGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 2 TGGCCA [CT]^GGCC[GA]
EcoRI 484 GAATTC G^AATTC
EcoT14I 254 CCATGG C^C[AT][AT]GG
HaeII 143 GGCGCT [GA]GCGC^[CT]
HincII 343 GTTGAC GT[CT]^[GA]AC
HindII 343 GTTGAC GT[CT]^[GA]AC
HinfI 180 GATTC G^A[ACGT]TC
MaeIII 412 GTGAC ^GT[ACGT]AC
MflI 441 AGATCC [GA]^GATC[CT]
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MslI 430 CACTACCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 499 CAGCTG C[AC]G^C[GT]G
MvnI 34 CGCG CG^CG
NaeI 562 GCCGGC GCC^GGC
NcoI 254 CCATGG C^CATGG
NgoMIV 562 GCCGGC G^CCGGC
PflMI 133 CCAGACCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 502 CTGCAG CTGCA^G
PvuII 499 CAGCTG CAG^CTG
RsaI 207 GTAC GT^AC
SfcI 502 CTGCAG C^T[GA][CT]AG
SfiI 552 GGCCTCCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StyI 254 CCATGG C^C[AT][AT]GG
TaqI 53 TCGA T^CGA
TfiI 180 GATTC G^A[AT]TC
Tsp45I 412 GTGAC ^GT[GC]AC
Tsp509I 485 AATT ^AATT
TthHB8I 53 TCGA T^CGA
Van91I 133 CCAGACCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 441 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwI 442 GATCC GGATC(4/5)
98 GGATC
AvaII 13 GGTCC G^G[AT]CC
383 GGACC
BmyI 235 GTGCCC G[AGT]GC[ACT]^C
427 GGGCAC
BseRI 70 GAGGAG GAGGAG(10/8)
79 GAGGAG
Bsp1286I 235 GTGCCC G[AGT]GC[ACT]^C
427 GGGCAC
BsrFI 225 GCCGGT [GA]^CCGG[CT]
562 GCCGGC
BssKI 156 CCAGG ^CC[ACGT]GG
558 CCAGG
BstNI 156 CCAGG CC^[AT]GG
558 CCAGG
CfoI 144 GCGC GCG^C
466 GCGC
Cfr10I 225 GCCGGT [GA]^CCGG[CT]
562 GCCGGC
EcoRII 156 CCAGG ^CC[AT]GG
558 CCAGG
HapII 226 CCGG C^CGG
563 CCGG
HhaI 144 GCGC GCG^C
466 GCGC
HinP1I 144 GCGC G^CGC
466 GCGC
HpaII 226 CCGG C^CGG
563 CCGG
HphI 195 GGTGA GGTGA(8/7)
228 GGTGA
MspI 226 CCGG C^CGG
563 CCGG
MvaI 156 CCAGG CC^[AT]GG
558 CCAGG
NspI 329 ACATGT [GA]CATG^[CT]
516 GCATGT
PpuMI 12 GGGTCCT [GA]G^G[AT]CC[CT]
382 AGGACCC
ScrFI 156 CCAGG CC^[ACGT]GG
558 CCAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AciI AluI BbvI BsaJI BsgI
BsiYI BslI Cac8I Cfr13I DdeI
DpnII DraII DsaI EcoO109I Fnu4HI
HaeIII ItaI MboI MboII MnlI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I TseI TspRI