Restriction map for
pFN21AB6859
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# NO CUT ENZYMES ( 183 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AvaI AviII BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstUI BstYI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DpnII DraI DraII DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII KasI KpnI Ksp632I
KspI MamI MboI MfeI MflI
MluI MroI MslI MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NdeII NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI Sau3AI SbfI
ScaI SexAI SfcI SfiI SfoI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AciI 185 GCGG CCGC(-3/-1)
AluI 357 AGCT AG^CT
AlwNI 149 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 255 GTGCTC G[AT]GC[AT]^C
AspI 508 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 490 GGACC G^G[AT]CC
AvrII 474 CCTAGG C^CTAGG
BalI 333 TGGCCA TGG^CCA
BfaI 475 CTAG C^TAG
BlnI 474 CCTAGG C^CTAGG
BmyI 255 GTGCTC G[AGT]GC[ACT]^C
BpmI 90 CTGGAG CTGGAG(16/14)
BsiHKAI 255 GTGCTC G[AT]GC[AT]^C
BsiYI 336 CCAGCCCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 336 CCAGCCCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 179 GAGAC GTCTC(1/5)
BsmFI 408 GGGAC GGGAC(10/14)
Bsp1286I 255 GTGCTC G[AGT]GC[ACT]^C
BstBI 212 TTCGAA TT^CGAA
BstEII 520 GGTCACC G^GT[ACGT]ACC
BstPI 520 GGTCACC G^GT[ACGT]ACC
DdeI 446 CTCAG C^T[ACGT]AG
EaeI 333 TGGCCA [CT]^GGCC[GA]
EcoO65I 520 GGTCACC G^GT[ACGT]ACC
FauI 185 GCGGG CCCGC(4/6)
HphI 522 TCACC GGTGA(8/7)
MaeI 475 CTAG C^TAG
MaeII 441 ACGT A^CGT
MluNI 333 TGGCCA TGG^CCA
MnlI 5 CCTC CCTC(7/6)
MscI 333 TGGCCA TGG^CCA
MspA1I 183 CAGCGG C[AC]G^C[GT]G
NlaIV 92 GGAGCC GG[ACGT]^[ACGT]CC
NspI 77 GCATGT [GA]CATG^[CT]
NspV 212 TTCGAA TT^CGAA
PflFI 508 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SfuI 212 TTCGAA TT^CGAA
SspI 26 AATATT AAT^ATT
Tth111I 508 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BbvI 247 GCTGC GCAGC(8/12)
265 GCTGC
BstF5I 37 CATCC GGATG(2/0)
113 GGATG
BstXI 8 CCAAATCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
341 CCAAGGTGGTGG
Cac8I 166 GCAAGC GC[ACGT]^[ACGT]GC
335 GCCAGC
Fnu4HI 247 GCTGC GC^[ACGT]GC
265 GCTGC
FokI 37 CATCC GGATG(9/13)
113 GGATG
HinfI 201 GATTC G^A[ACGT]TC
288 GATTC
ItaI 247 GCTGC GC^[ACGT]GC
265 GCTGC
MaeIII 514 GTCAC ^GT[ACGT]AC
521 GTCAC
MboII 296 GAAGA GAAGA(8/7)
367 GAAGA
MseI 223 TTAA T^TAA
424 TTAA
TaqI 99 TCGA T^CGA
213 TCGA
TfiI 201 GATTC G^A[AT]TC
288 GATTC
Tru9I 223 TTAA T^TAA
424 TTAA
TseI 247 GCTGC G^C[AT]GC
265 GCTGC
Tsp45I 514 GTCAC ^GT[GC]AC
521 GTCAC
Tsp509I 426 AATT ^AATT
504 AATT
TspRI 253 CAGTG CA[GC]TG[ACGT][ACGT]^
516 CACTG
TthHB8I 99 TCGA T^CGA
213 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
#########################################
AfaI BsaJI BsrI BssKI BstNI
Cfr13I EcoRII EcoT14I HaeIII MvaI
MwoI NlaIII RsaI Sau96I ScrFI
SfaNI StyI