Restriction map for
pFN21AB6867
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# NO CUT ENZYMES ( 180 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI AluI Alw44I
AlwI AocI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DpnII DraI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MaeII MboI MfeI MflI
MluI MluNI MroI MscI MseI
MspA1I MunI MvnI NaeI NarI
NdeII NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI Sau3AI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI SspBI
SspI StuI SwaI TfiI Tru9I
Tsp509I Tth111I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AciI 433 CCGC CCGC(-3/-1)
AlwNI 202 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 7 AGCGCT AGC^GCT
BanI 504 GGTGCC G^G[CT][GA]CC
BbvI 226 GCTGC GCAGC(8/12)
BglI 507 GCCCACTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 19 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 480 GAGACC GGTCTC(1/5)
BsmAI 480 GAGAC GTCTC(1/5)
BspMI 414 ACCTGC ACCTGC(4/8)
BsrI 406 CCAGT ACTGG(1/-1)
CfoI 8 GCGC GCG^C
Eco47III 7 AGCGCT AGC^GCT
EcoNI 274 CCTCCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 437 CCATGG C^C[AT][AT]GG
HaeII 7 AGCGCT [GA]GCGC^[CT]
HhaI 8 GCGC GCG^C
HinP1I 8 GCGC G^CGC
MaeIII 80 GTGAC ^GT[ACGT]AC
MamI 19 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 437 CCATGG C^CATGG
SapI 190 GAAGAGC GCTCTTC(1/4)
SbfI 277 CCTGCAGG CCTGCA^GG
Sse8387I 277 CCTGCAGG CCTGCA^GG
StyI 437 CCATGG C^C[AT][AT]GG
TaqI 528 TCGA T^CGA
TseI 226 GCTGC G^C[AT]GC
Tsp45I 80 GTGAC ^GT[GC]AC
TthHB8I 528 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AvaII 316 GGTCC G^G[AT]CC
412 GGACC
BcnI 313 CCGGG CC^[GC]GG
517 CCCGG
BsmFI 411 GGGAC GGGAC(10/14)
494 GTCCC
Cac8I 195 GCAGGC GC[ACGT]^[ACGT]GC
339 GCCAGC
DdeI 28 CTCAG C^T[ACGT]AG
200 CTCAG
DsaI 14 CCGTGG C^C[GA][CT]GG
437 CCATGG
EarI 49 CTCTTC CTCTTC(1/4)
190 GAAGAG
Fnu4HI 226 GCTGC GC^[ACGT]GC
432 GCCGC
HapII 313 CCGG C^CGG
518 CCGG
HinfI 109 GACTC G^A[ACGT]TC
525 GACTC
HpaII 313 CCGG C^CGG
518 CCGG
HphI 58 TCACC GGTGA(8/7)
101 TCACC
ItaI 226 GCTGC GC^[ACGT]GC
432 GCCGC
Ksp632I 49 CTCTTC CTCTTC(1/4)
190 GAAGAG
MboII 190 GAAGA GAAGA(8/7)
50 TCTTC
MspI 313 CCGG C^CGG
518 CCGG
MwoI 453 GCCAATGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
507 GCCCACTCGGC
NciI 313 CCGGG CC^[GC]GG
517 CCCGG
NdeI 349 CATATG CA^TATG
448 CATATG
PleI 109 GACTC GAGTC(4/5)
525 GACTC
PpuMI 315 GGGTCCT [GA]G^G[AT]CC[CT]
411 GGGACCT
PstI 135 CTGCAG CTGCA^G
278 CTGCAG
SfcI 135 CTGCAG C^T[GA][CT]AG
278 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
ApaI BanII BmyI BsaJI BseRI
BsiYI BslI Bsp1286I BssKI BstF5I
BstNI Cfr13I DraII EcoO109I EcoRII
FokI HaeIII MnlI MslI MvaI
NlaIII NlaIV Sau96I ScrFI SfaNI
TspRI