Restriction map for
pFN21AB6828
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# NO CUT ENZYMES ( 189 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BamHI BanII
BbeI BbrPI BbsI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstYI BstZ17I Bsu36I CelII
CfoI Cfr10I Cfr13I ClaI CpoI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HpaII
KasI KpnI KspI MaeII MamI
MfeI MflI MluI MroI MspA1I
MspI MunI MvnI NaeI NarI
NciI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
Sau96I SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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BalI 340 TGGCCA TGG^CCA
BanI 483 GGTGCC G^G[CT][GA]CC
BbvCI 419 CCTCAGC CCTCAGC(-5/-2)
BbvI 433 GCTGC GCAGC(8/12)
BfaI 95 CTAG C^TAG
BmyI 484 GTGCCC G[AGT]GC[ACT]^C
BpmI 37 CTGGAG CTGGAG(16/14)
BseRI 21 CTCCTC GAGGAG(10/8)
BsmBI 40 GAGACG CGTCTC(1/5)
BsmFI 226 GGGAC GGGAC(10/14)
Bsp1286I 484 GTGCCC G[AGT]GC[ACT]^C
BsrBI 196 CCGCTC CCGCTC(-3/-3)
BsrDI 408 CATTGC GCAATG(2/0)
BssKI 36 CCTGG ^CC[ACGT]GG
BssSI 24 CTCGTG CACGAG(-5/-1)
BstNI 36 CCTGG CC^[AT]GG
BstXI 366 CCATAGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 397 GCAAGC GC[ACGT]^[ACGT]GC
DpnII 15 GATC ^GATC
DraI 471 TTTAAA TTT^AAA
DsaI 290 CCATGG C^C[GA][CT]GG
EaeI 340 TGGCCA [CT]^GGCC[GA]
EcoRII 36 CCTGG ^CC[AT]GG
Fnu4HI 433 GCTGC GC^[ACGT]GC
HaeIII 341 GGCC GG^CC
HinfI 514 GAGTC G^A[ACGT]TC
HphI 186 GGTGA GGTGA(8/7)
ItaI 433 GCTGC GC^[ACGT]GC
MaeI 95 CTAG C^TAG
MaeIII 323 GTGAC ^GT[ACGT]AC
MboI 15 GATC ^GATC
MluNI 340 TGGCCA TGG^CCA
MscI 340 TGGCCA TGG^CCA
MseI 472 TTAA T^TAA
MvaI 36 CCTGG CC^[AT]GG
NcoI 290 CCATGG C^CATGG
NdeI 489 CATATG CA^TATG
NdeII 15 GATC ^GATC
NlaIII 291 CATG CATG^
PleI 514 GAGTC GAGTC(4/5)
Sau3AI 15 GATC ^GATC
ScrFI 36 CCTGG CC^[ACGT]GG
TaqI 4 TCGA T^CGA
Tru9I 472 TTAA T^TAA
TseI 433 GCTGC G^C[AT]GC
Tsp45I 323 GTGAC ^GT[GC]AC
Tsp509I 47 AATT ^AATT
TthHB8I 4 TCGA T^CGA
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# TWO CUT ENZYMES ( 18 enzymes) #
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AciI 196 CCGC CCGC(-3/-1)
295 GCGG
BsaJI 290 CCATGG C^C[ACGT][ACGT]GG
479 CCAAGG
BsiYI 63 CCCATTAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
196 CCGCTCTGTGG
BslI 63 CCCATTAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
196 CCGCTCTGTGG
BsmAI 40 GAGAC GTCTC(1/5)
175 GTCTC
BsrI 343 CCAGT ACTGG(1/-1)
385 CCAGT
BstF5I 554 CATCC GGATG(2/0)
108 GGATG
DdeI 267 CTTAG C^T[ACGT]AG
420 CTCAG
EarI 118 GAAGAG CTCTTC(1/4)
511 GAAGAG
EcoT14I 290 CCATGG C^C[AT][AT]GG
479 CCAAGG
FokI 554 CATCC GGATG(9/13)
108 GGATG
Ksp632I 118 GAAGAG CTCTTC(1/4)
511 GAAGAG
MboII 118 GAAGA GAAGA(8/7)
511 GAAGA
MslI 354 CATAGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
408 CATTGCTGTG
NlaIV 381 GGTTCC GG[ACGT]^[ACGT]CC
483 GGTGCC
SfaNI 436 GCATC GCATC(5/9)
553 GCATC
StyI 290 CCATGG C^C[AT][AT]GG
479 CCAAGG
TspRI 359 CAGTG CA[GC]TG[ACGT][ACGT]^
386 CAGTG
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# 3 OR MORE CUT ENZYMES ( 3 enzymes) #
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AluI MnlI MwoI