Restriction map for
pFN21AB6802
##################################
# NO CUT ENZYMES ( 163 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmFI BsmI BsoBI BspDI
BspEI BspLU11I BspMI BsrDI BssHII
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII CfoI ClaI
CpoI DraI DraII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I MaeI MamI MluI
MluNI MroI MscI NarI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StyI SwaI TaqI
Tth111I TthHB8I Van91I XbaI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AlwNI 442 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 426 GGTCC G^G[AT]CC
BanII 468 GAGCCC G[GA]GC[CT]^C
BbsI 545 GAAGAC GAAGAC(2/6)
BmyI 468 GAGCCC G[AGT]GC[ACT]^C
BpuAI 545 GAAGAC GAAGAC(2/6)
BsaI 273 GGTCTC GGTCTC(1/5)
BsgI 343 CTGCAC GTGCAG(16/14)
BsmBI 62 GAGACG CGTCTC(1/5)
Bsp1286I 468 GAGCCC G[AGT]GC[ACT]^C
BspHI 308 TCATGA T^CATGA
BsrBI 509 CCGCTC CCGCTC(-3/-3)
BsrFI 230 GCCGGC [GA]^CCGG[CT]
BsrI 291 ACTGG ACTGG(1/-1)
BssSI 223 CACGAG CACGAG(-5/-1)
BstAPI 345 GCACACCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 230 GCCGGC [GA]^CCGG[CT]
DdeI 541 CTCAG C^T[ACGT]AG
DraIII 346 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 237 CCGCGG C^C[GA][CT]GG
EcoRI 518 GAATTC G^AATTC
HgaI 64 GACGC GACGC(5/10)
HinfI 169 GATTC G^A[ACGT]TC
KspI 237 CCGCGG CCGC^GG
MaeII 383 ACGT A^CGT
MfeI 73 CAATTG C^AATTG
MslI 112 CACGATAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 73 CAATTG C^AATTG
NaeI 230 GCCGGC GCC^GGC
NgoMIV 230 GCCGGC G^CCGGC
NlaIV 233 GGCTCC GG[ACGT]^[ACGT]CC
RcaI 308 TCATGA T^CATGA
SacII 237 CCGCGG CCGC^GG
StuI 124 AGGCCT AGG^CCT
TfiI 169 GATTC G^A[AT]TC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 66 CGCG CG^CG
238 CGCG
AcsI 214 AAATTC [GA]^AATT[CT]
518 GAATTC
AfaI 36 GTAC GT^AC
256 GTAC
AluI 247 AGCT AG^CT
401 AGCT
AlwI 21 GATCC GGATC(4/5)
365 GATCC
ApoI 214 AAATTC [GA]^AATT[CT]
518 GAATTC
BbvI 163 GCAGC GCAGC(8/12)
248 GCTGC
BpmI 276 CTCCAG CTGGAG(16/14)
403 CTCCAG
BsaJI 237 CCGCGG C^C[ACGT][ACGT]GG
269 CCGGGG
Bsp1407I 35 TGTACA T^GTACA
255 TGTACA
BsrGI 35 TGTACA T^GTACA
255 TGTACA
BstUI 66 CGCG CG^CG
238 CGCG
BstYI 20 AGATCC [GA]^GATC[CT]
364 AGATCC
Cac8I 230 GCCGGC GC[ACGT]^[ACGT]GC
354 GCGTGC
Cfr13I 426 GGTCC G^G[ACGT]CC
533 GGGCC
EaeI 266 TGGCCG [CT]^GGCC[GA]
506 TGGCCG
MflI 20 AGATCC [GA]^GATC[CT]
364 AGATCC
MseI 143 TTAA T^TAA
212 TTAA
MspA1I 164 CAGCGG C[AC]G^C[GT]G
237 CCGCGG
MvnI 66 CGCG CG^CG
238 CGCG
NlaIII 309 CATG CATG^
421 CATG
RsaI 36 GTAC GT^AC
256 GTAC
Sau96I 426 GGTCC G^G[ACGT]CC
533 GGGCC
SspBI 35 TGTACA T^GTACA
255 TGTACA
Tru9I 143 TTAA T^TAA
212 TTAA
TseI 163 GCAGC G^C[AT]GC
248 GCTGC
XhoII 20 AGATCC [GA]^GATC[CT]
364 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI BcnI BsiYI BslI BsmAI
BssKI BstF5I BstNI DpnII EcoRII
Fnu4HI FokI HaeIII HapII HpaII
HphI ItaI MaeIII MboI MboII
MnlI MspI MvaI MwoI NciI
NdeII Sau3AI ScrFI SfaNI Tsp45I
Tsp509I TspRI XcmI