(Circular) (Six-base) MAPSORT of: pFN21AB6802.seq Check: 1475 from: 1 to: 5257 pFN21AB6802 5257 bp With 182 enzymes: SgfI * October 20, 2009 10:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3067 278 Size: 53 83 186 936 1531 2468 Fragments arranged by size: 2468 1531 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2600 1937 Size: 663 4594 Acc65I G'GTAC_C Cuts at: 2578 2578 Size: 5257 AclI AA'CG_TT Cuts at: 1563 3384 3757 1563 Size: 1821 373 3063 Fragments arranged by size: 3063 1821 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2474 2549 3320 4704 4972 1632 Size: 842 75 771 1384 268 1917 Fragments arranged by size: 1917 1384 842 771 268 75 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5060 AflIII A'CryG_T Cuts at: 1203 4156 5133 1203 Size: 2953 977 1327 Fragments arranged by size: 2953 1327 977 AgeI A'CCGG_T Cuts at: 4088 4088 Size: 5257 AhdI GACnn_n'nnGTC Cuts at: 3986 3986 Size: 5257 AleI CACnn'nnGTG Cuts at: 1166 2121 1166 Size: 955 4302 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5257 AlwNI CAG_nnn'CTG Cuts at: 2452 3000 4572 2452 Size: 548 1572 3137 Fragments arranged by size: 3137 1572 548 ApaLI G'TGCA_C Cuts at: 3314 4470 3314 Size: 1156 4101 ApoI r'AATT_y Cuts at: 1493 2219 2523 2566 2792 2944 5053 1493 Size: 726 304 43 226 152 2109 1697 Fragments arranged by size: 2109 1697 726 304 226 152 43 AseI AT'TA_AT Cuts at: 160 1032 3811 160 Size: 872 2779 1606 Fragments arranged by size: 2779 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5257 AvaI C'yCGr_G Cuts at: 1870 1943 2582 1870 Size: 73 639 4545 Fragments arranged by size: 4545 639 73 BamHI G'GATC_C Cuts at: 2587 2954 2587 Size: 367 4890 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2578 4033 618 Size: 325 217 627 791 1455 1842 Fragments arranged by size: 1842 1455 791 627 325 217 BanII G_rGCy'C Cuts at: 1391 2477 2576 1391 Size: 1086 99 4072 Fragments arranged by size: 4072 1086 99 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2557 928 Size: 971 658 3628 Fragments arranged by size: 3628 971 658 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3448 3482 1903 Size: 34 1511 34 3678 Fragments arranged by size: 3678 1511 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3149 4359 3149 Size: 1210 4047 BclI T'GATC_A Cuts at: 1548 1548 Size: 5257 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3868 136 Size: 107 122 71 631 605 2196 1525 Fragments arranged by size: 2196 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5252 5252 Size: 5257 BlpI GC'TnA_GC Cuts at: 2674 2674 Size: 5257 Bme1580I G_kGCm'C Cuts at: 1790 3318 4474 1790 Size: 1528 1156 2573 Fragments arranged by size: 2573 1528 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5257 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2988 3946 442 Size: 416 53 83 399 24 1571 958 1753 Fragments arranged by size: 1753 1571 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5257 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2266 2393 3917 1239 Size: 1027 127 1524 2579 Fragments arranged by size: 2579 1524 1027 127 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3382 4247 4545 3382 Size: 865 298 4094 Fragments arranged by size: 4094 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2284 3920 882 Size: 617 785 1636 2219 Fragments arranged by size: 2219 1636 785 617 BsaAI yAC'GTr Cuts at: 493 1204 3048 493 Size: 711 1844 2702 Fragments arranged by size: 2702 1844 711 BsaBI GATnn'nnATC Cuts at: 5251 5251 Size: 5257 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3064 3446 4081 275 Size: 53 83 186 936 1531 382 635 1451 Fragments arranged by size: 1531 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3690 4088 4362 4509 4920 3690 Size: 398 274 147 411 4027 Fragments arranged by size: 4027 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2359 2389 1178 Size: 30 165 30 956 30 4046 Fragments arranged by size: 4046 956 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5257 BseYI C'CCAG_C Cuts at: 2164 2334 2363 4460 4956 2164 Size: 170 29 2097 496 2465 Fragments arranged by size: 2465 2097 496 170 29 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2333 1163 Size: 573 597 4087 Fragments arranged by size: 4087 597 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2707 3468 4496 1540 Size: 275 186 706 761 1028 2301 Fragments arranged by size: 2301 1028 761 706 275 186 BsiHKAI G_wGCw'C Cuts at: 3318 3403 4474 3318 Size: 85 1071 4101 Fragments arranged by size: 4101 1071 85 BsmI GAATG_Cn' Cuts at: 2768 2861 2768 Size: 93 5164 BsmBI CGTCTCn'nnnn_ Cuts at: 2061 2061 Size: 5257 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2477 2576 3318 3403 4474 1391 Size: 399 687 99 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 687 399 99 85 BspEI T'CCGG_A Cuts at: 4920 4920 Size: 5257 BspHI T'CATG_A Cuts at: 2313 3146 4876 2313 Size: 833 1730 2694 Fragments arranged by size: 2694 1730 833 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2612 844 Size: 1768 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2516 3144 1394 Size: 429 693 628 3507 Fragments arranged by size: 3507 693 628 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3752 3926 65 Size: 1183 2504 174 1396 Fragments arranged by size: 2504 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2235 3901 4088 1700 Size: 110 425 1666 187 2869 Fragments arranged by size: 2869 1666 425 187 110 BsrGI T'GTAC_A Cuts at: 96 2040 2260 96 Size: 1944 220 3093 Fragments arranged by size: 3093 1944 220 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5257 BssSI C'ACGA_G Cuts at: 2228 3317 4329 2228 Size: 1089 1012 3156 Fragments arranged by size: 3156 1089 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2356 794 Size: 1562 3695 BstBI TT'CG_AA Cuts at: 4061 4061 Size: 5257 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5257 BstYI r'GATC_y Cuts at: 1972 2025 2369 2587 2954 3339 3356 4808 4894 Size: 53 344 218 367 385 17 1452 86 Cuts at: 4894 4906 5252 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 344 218 86 53 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2242 513 Size: 552 939 238 3528 Fragments arranged by size: 3528 939 552 238 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4518 Fragments arranged by size: 4518 683 56 BtsI GCAGTG_nn' Cuts at: 2263 2453 2773 3567 3587 2263 Size: 190 320 794 20 3933 Fragments arranged by size: 3933 794 320 190 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5257 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5222 DraI TTT'AAA Cuts at: 2561 2561 Size: 5257 DraIII CAC_nnn'GTG Cuts at: 2356 2356 Size: 5257 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4264 809 Size: 507 2948 1802 Fragments arranged by size: 2948 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2271 2511 2704 Size: 54 1425 50 275 18 441 240 193 Cuts at: 2704 3593 5222 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 441 275 240 193 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2704 1537 Size: 275 892 4090 Fragments arranged by size: 4090 892 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3187 1353 Size: 1834 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3844 4218 4364 387 Size: 1064 138 2255 374 146 1280 Fragments arranged by size: 2255 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2266 2393 2474 2549 3320 3917 4704 Size: 393 634 127 81 75 771 597 787 Cuts at: 4704 4972 1239 Size: 268 1524 Fragments arranged by size: 1524 787 771 634 597 393 268 127 81 75 EcoRI G'AATT_C Cuts at: 2523 2566 2944 2523 Size: 43 378 4836 Fragments arranged by size: 4836 378 43 FspI TGC'GCA Cuts at: 3763 3763 Size: 5257 HaeII r_GCGC'y Cuts at: 1678 4404 1678 Size: 2726 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2165 2197 3898 3930 3972 Size: 32 135 32 593 32 1701 32 42 Cuts at: 3972 4004 1373 Size: 32 2626 Fragments arranged by size: 2626 1701 593 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2601 2847 669 Size: 988 281 663 246 3079 Fragments arranged by size: 3079 988 663 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5257 HpaI GTT'AAC Cuts at: 2847 2847 Size: 5257 KpnI G_GTAC'C Cuts at: 2582 2582 Size: 5257 MfeI C'AATT_G Cuts at: 2078 2856 2078 Size: 778 4479 MluI A'CGCG_T Cuts at: 5133 5133 Size: 5257 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2118 4371 4555 1305 Size: 813 2253 184 2007 Fragments arranged by size: 2253 2007 813 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3435 Fragments arranged by size: 3435 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2121 3215 3574 3733 518 Size: 648 955 1094 359 159 2042 Fragments arranged by size: 2042 1094 955 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2171 2244 2673 3350 4498 4743 1606 Size: 565 73 429 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 565 429 245 73 NaeI GCC'GGC Cuts at: 2237 2237 Size: 5257 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3766 Fragments arranged by size: 3766 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5257 NgoMIV G'CCGG_C Cuts at: 2235 2235 Size: 5257 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5257 NotI GC'GGCC_GC Cuts at: 2704 2704 Size: 5257 NspI r_CATG'y Cuts at: 1132 1229 2615 4160 1132 Size: 97 1386 1545 2229 Fragments arranged by size: 2229 1545 1386 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5257 PciI A'CATG_T Cuts at: 4156 4156 Size: 5257 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5257 PfoI T'CCnGG_A Cuts at: 2282 2409 2518 2282 Size: 127 109 5021 Fragments arranged by size: 5021 127 109 PmeI GTTT'AAAC Cuts at: 2561 2561 Size: 5257 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3326 3358 4839 4871 3326 Size: 32 1481 32 3712 Fragments arranged by size: 3712 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5257 PsiI TTA'TAA Cuts at: 2827 2827 Size: 5257 PstI C_TGCA'G Cuts at: 830 2609 830 Size: 1779 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5257 SacII CC_GC'GG Cuts at: 2245 2245 Size: 5257 SalI G'TCGA_C Cuts at: 1936 2599 1936 Size: 663 4594 SbfI CC_TGCA'GG Cuts at: 2609 2609 Size: 5257 ScaI AGT'ACT Cuts at: 3505 5217 3505 Size: 1712 3545 SfcI C'TryA_G Cuts at: 826 1045 2605 3740 4421 4612 826 Size: 219 1560 1135 681 191 1471 Fragments arranged by size: 1560 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5257 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5257 SmaI CCC'GGG Cuts at: 1872 2584 1872 Size: 712 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3361 4262 4524 820 Size: 197 926 1418 901 262 1553 Fragments arranged by size: 1553 1418 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3048 493 Size: 2555 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5257 SphI G_CATG'C Cuts at: 1132 2615 1132 Size: 1483 3774 SspI AAT'ATT Cuts at: 5 52 3181 5 Size: 47 3129 2081 Fragments arranged by size: 3129 2081 47 StuI AGG'CCT Cuts at: 1517 2131 1517 Size: 614 4643 StyI C'CwwG_G Cuts at: 513 1065 2004 2696 513 Size: 552 939 692 3074 Fragments arranged by size: 3074 939 692 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3284 3301 3454 3639 4084 325 Size: 1255 1704 17 153 185 445 1498 Fragments arranged by size: 1704 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2040 2260 3503 Size: 274 80 33 51 1443 63 220 1243 Cuts at: 3503 5215 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1243 274 220 138 80 63 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2359 2391 5217 5249 2359 Size: 32 2826 32 2367 Fragments arranged by size: 2826 2367 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5257 XbaI T'CTAG_A Cuts at: 2593 2593 Size: 5257 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2134 2386 2506 2134 Size: 252 120 4885 Fragments arranged by size: 4885 252 120 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5257 XmaI C'CCGG_G Cuts at: 1870 2582 1870 Size: 712 4545 XmnI GAAnn'nnTTC Cuts at: 3386 3386 Size: 5257 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3065 276 Size: 53 83 186 936 1531 2468 Fragments arranged by size: 2468 1531 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI