Restriction map for
pFN21AB6802
(Linear) (Six-base) MAPSORT of: KIBB6802.seq Check: 4053 from: 1 to: 552
KIBB6802.orf
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 469 544 552
Size: 469 75 8
Fragments arranged by size:
469 75 8
AleI CACnn'nnGTG
Cuts at: 0 116 552
Size: 116 436
AlwNI CAG_nnn'CTG
Cuts at: 0 447 552
Size: 447 105
ApoI r'AATT_y
Cuts at: 0 214 518 552
Size: 214 304 34
Fragments arranged by size:
304 214 34
BanII G_rGCy'C
Cuts at: 0 472 552
Size: 472 80
BbsI GAAGACnn'nnnn_
Cuts at: 0 552
Size: 552
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 261 388 552
Size: 261 127 164
Fragments arranged by size:
261 164 127
BsaI GGTCTCn'nnnn_
Cuts at: 0 279 552
Size: 279 273
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 354 384 552
Size: 354 30 168
Fragments arranged by size:
354 168 30
BseYI C'CCAG_C
Cuts at: 0 159 329 358 552
Size: 159 170 29 194
Fragments arranged by size:
194 170 159 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 552
Size: 328 224
BsmBI CGTCTCn'nnnn_
Cuts at: 0 56 552
Size: 56 496
Bsp1286I G_dGCh'C
Cuts at: 0 472 552
Size: 472 80
BspHI T'CATG_A
Cuts at: 0 308 552
Size: 308 244
BsrBI CCG'CTC
Cuts at: 0 511 552
Size: 511 41
BsrFI r'CCGG_y
Cuts at: 0 230 552
Size: 230 322
BsrGI T'GTAC_A
Cuts at: 0 35 255 552
Size: 35 220 297
Fragments arranged by size:
297 220 35
BssSI C'ACGA_G
Cuts at: 0 223 552
Size: 223 329
BstAPI GCAn_nnn'nTGC
Cuts at: 0 351 552
Size: 351 201
BstYI r'GATC_y
Cuts at: 0 20 364 552
Size: 20 344 188
Fragments arranged by size:
344 188 20
BtgI C'CryG_G
Cuts at: 0 237 552
Size: 237 315
BtsI GCAGTG_nn'
Cuts at: 0 258 448 552
Size: 258 190 104
Fragments arranged by size:
258 190 104
DraIII CAC_nnn'GTG
Cuts at: 0 351 552
Size: 351 201
EaeI y'GGCC_r
Cuts at: 0 266 506 552
Size: 266 240 46
Fragments arranged by size:
266 240 46
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 261 388 469 544 552
Size: 261 127 81 75 8
Fragments arranged by size:
261 127 81 75 8
EcoRI G'AATT_C
Cuts at: 0 518 552
Size: 518 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 160 192 552
Size: 160 32 360
Fragments arranged by size:
360 160 32
MfeI C'AATT_G
Cuts at: 0 73 552
Size: 73 479
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 552
Size: 113 439
MslI CAynn'nnrTG
Cuts at: 0 116 552
Size: 116 436
MspA1I CmG'CkG
Cuts at: 0 166 239 552
Size: 166 73 313
Fragments arranged by size:
313 166 73
NaeI GCC'GGC
Cuts at: 0 232 552
Size: 232 320
NgoMIV G'CCGG_C
Cuts at: 0 230 552
Size: 230 322
PfoI T'CCnGG_A
Cuts at: 0 277 404 513 552
Size: 277 127 109 39
Fragments arranged by size:
277 127 109 39
SacII CC_GC'GG
Cuts at: 0 240 552
Size: 240 312
StuI AGG'CCT
Cuts at: 0 126 552
Size: 126 426
TatI w'GTAC_w
Cuts at: 0 35 255 552
Size: 35 220 297
Fragments arranged by size:
297 220 35
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 354 386 552
Size: 354 32 166
Fragments arranged by size:
354 166 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 129 381 501 552
Size: 129 252 120 51
Fragments arranged by size:
252 129 120 51
Enzymes that do cut:
AcuI AleI AlwNI ApoI BanII BbsI BpmI BsaI
BsaXI BseYI BsgI BsmBI Bsp1286I BspHI BsrBI BsrFI
BsrGI BssSI BstAPI BstYI BtgI BtsI DraIII EaeI
Eco57MI EcoRI Hin4I MfeI MmeI MslI MspA1I NaeI
NgoMIV PfoI SacII StuI TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaAI BsaBI
BsaHI BsaWI BseRI BsiEI BsiHKAI BsiWI BsmI BspEI
BspMI BsrDI BssHII BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DrdI EagI EarI EciI
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MluI MscI
NarI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StyI SwaI TaqII Tth111I
XbaI XhoI XmaI XmnI ZraI