(Circular) (Six-base) MAPSORT of: pFN21AB6766.seq Check: 9459 from: 1 to: 5149 pFN21AB6766 5149 bp With 182 enzymes: SgfI * October 20, 2009 10:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2959 278 Size: 53 83 186 936 1423 2468 Fragments arranged by size: 2468 1423 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2392 2492 1937 Size: 455 100 4594 Fragments arranged by size: 4594 455 100 Acc65I G'GTAC_C Cuts at: 2470 2470 Size: 5149 AclI AA'CG_TT Cuts at: 1563 3276 3649 1563 Size: 1713 373 3063 Fragments arranged by size: 3063 1713 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2225 3212 4596 4864 1632 Size: 593 987 1384 268 1917 Fragments arranged by size: 1917 1384 987 593 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4952 AflIII A'CryG_T Cuts at: 1203 4048 5025 1203 Size: 2845 977 1327 Fragments arranged by size: 2845 1327 977 AgeI A'CCGG_T Cuts at: 3980 3980 Size: 5149 AhdI GACnn_n'nnGTC Cuts at: 3878 3878 Size: 5149 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5149 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5149 AlwNI CAG_nnn'CTG Cuts at: 2215 2892 4464 2215 Size: 677 1572 2900 Fragments arranged by size: 2900 1572 677 ApaLI G'TGCA_C Cuts at: 3206 4362 3206 Size: 1156 3993 ApoI r'AATT_y Cuts at: 1493 2251 2458 2684 2836 4945 1493 Size: 758 207 226 152 2109 1697 Fragments arranged by size: 2109 1697 758 226 207 152 AseI AT'TA_AT Cuts at: 160 1032 3703 160 Size: 872 2671 1606 Fragments arranged by size: 2671 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5149 AvaI C'yCGr_G Cuts at: 1870 1943 2061 2474 1870 Size: 73 118 413 4545 Fragments arranged by size: 4545 413 118 73 BamHI G'GATC_C Cuts at: 2479 2846 2479 Size: 367 4782 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2056 2470 3925 618 Size: 325 217 627 269 414 1455 1842 Fragments arranged by size: 1842 1455 627 414 325 269 217 BanII G_rGCy'C Cuts at: 1391 2022 2468 1391 Size: 631 446 4072 Fragments arranged by size: 4072 631 446 BbeI G_GCGC'C Cuts at: 2060 2060 Size: 5149 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2169 928 Size: 971 270 3908 Fragments arranged by size: 3908 971 270 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3340 3374 1903 Size: 34 50 34 1319 34 3678 Fragments arranged by size: 3678 1319 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3041 4251 3041 Size: 1210 3939 BclI T'GATC_A Cuts at: 1548 1548 Size: 5149 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2065 3760 136 Size: 107 122 71 631 605 393 1695 1525 Fragments arranged by size: 1695 1525 631 605 393 122 107 71 BglII A'GATC_T Cuts at: 5144 5144 Size: 5149 BlpI GC'TnA_GC Cuts at: 2566 2566 Size: 5149 Bme1580I G_kGCm'C Cuts at: 1790 2148 3210 4366 1790 Size: 358 1062 1156 2573 Fragments arranged by size: 2573 1156 1062 358 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5149 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2143 2880 3838 Size: 416 53 83 399 24 726 737 958 Cuts at: 3838 442 Size: 1753 Fragments arranged by size: 1753 958 737 726 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5149 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2388 3809 1239 Size: 1149 1421 2579 Fragments arranged by size: 2579 1421 1149 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3274 4139 4437 3274 Size: 865 298 3986 Fragments arranged by size: 3986 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2267 3812 882 Size: 617 768 1545 2219 Fragments arranged by size: 2219 1545 768 617 BsaAI yAC'GTr Cuts at: 493 1204 2940 493 Size: 711 1736 2702 Fragments arranged by size: 2702 1736 711 BsaBI GATnn'nnATC Cuts at: 5143 5143 Size: 5149 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2057 2956 3338 3973 Size: 53 83 186 936 524 899 382 635 Cuts at: 3973 275 Size: 1451 Fragments arranged by size: 1451 936 899 635 524 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3582 3980 4254 4401 4812 3582 Size: 398 274 147 411 3919 Fragments arranged by size: 3919 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2085 2115 1178 Size: 30 165 30 682 30 4212 Fragments arranged by size: 4212 682 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5149 BseYI C'CCAG_C Cuts at: 2257 2433 4352 4848 2257 Size: 176 1919 496 2558 Fragments arranged by size: 2558 1919 496 176 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4576 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2014 2599 3360 4388 1540 Size: 275 186 13 585 761 1028 2301 Fragments arranged by size: 2301 1028 761 585 275 186 13 BsiHKAI G_wGCw'C Cuts at: 2022 2168 3210 3295 4366 2022 Size: 146 1042 85 1071 2805 Fragments arranged by size: 2805 1071 1042 146 85 BsmI GAATG_Cn' Cuts at: 2660 2753 2660 Size: 93 5056 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 2148 2168 2468 3210 3295 4366 Size: 399 232 126 20 300 742 85 1071 Cuts at: 4366 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 300 232 126 85 20 BspEI T'CCGG_A Cuts at: 4812 4812 Size: 5149 BspHI T'CATG_A Cuts at: 3038 4768 3038 Size: 1730 3419 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2504 844 Size: 1660 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2072 3036 1394 Size: 429 249 964 3507 Fragments arranged by size: 3507 964 429 249 BsrDI GCAATG_nn' Cuts at: 65 1248 3644 3818 65 Size: 1183 2396 174 1396 Fragments arranged by size: 2396 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3793 3980 1700 Size: 110 1983 187 2869 Fragments arranged by size: 2869 1983 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5149 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5149 BssSI C'ACGA_G Cuts at: 3209 4221 3209 Size: 1012 4137 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5149 BstBI TT'CG_AA Cuts at: 3953 3953 Size: 5149 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5149 BstXI CCAn_nnnn'nTGG Cuts at: 2314 2314 Size: 5149 BstYI r'GATC_y Cuts at: 1972 2479 2846 3231 3248 4700 4786 4798 5144 Size: 507 367 385 17 1452 86 12 346 Cuts at: 5144 1972 Size: 1977 Fragments arranged by size: 1977 1452 507 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4597 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4410 Fragments arranged by size: 4410 683 56 BtsI GCAGTG_nn' Cuts at: 2665 3459 3479 2665 Size: 794 20 4335 Fragments arranged by size: 4335 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5149 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5114 DraI TTT'AAA Cuts at: 2453 2453 Size: 5149 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4156 809 Size: 507 2840 1802 Fragments arranged by size: 2840 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2011 2596 3485 Size: 54 1425 50 275 18 181 585 889 Cuts at: 3485 5114 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 585 275 181 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2011 2596 1537 Size: 275 199 585 4090 Fragments arranged by size: 4090 585 275 199 EarI CTCTTCn'nnn_ Cuts at: 1353 3079 1353 Size: 1726 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2106 3736 4110 4256 387 Size: 1064 138 517 1630 374 146 1280 Fragments arranged by size: 1630 1280 1064 517 374 146 138 EcoICRI GAG'CTC Cuts at: 2020 2020 Size: 5149 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2225 2388 3212 3809 4596 4864 1239 Size: 393 593 163 824 597 787 268 1524 Fragments arranged by size: 1524 824 787 597 593 393 268 163 EcoO109I rG'GnC_Cy Cuts at: 2311 2311 Size: 5149 EcoRI G'AATT_C Cuts at: 2458 2836 2458 Size: 378 4771 FspI TGC'GCA Cuts at: 3655 3655 Size: 5149 HaeII r_GCGC'y Cuts at: 1678 2042 2060 4296 1678 Size: 364 18 2236 2531 Fragments arranged by size: 2531 2236 364 18 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2211 2243 3790 3822 3864 Size: 32 135 32 639 32 1547 32 42 Cuts at: 3864 3896 1373 Size: 32 2626 Fragments arranged by size: 2626 1547 639 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2493 2739 669 Size: 988 281 555 246 3079 Fragments arranged by size: 3079 988 555 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5149 HpaI GTT'AAC Cuts at: 2739 2739 Size: 5149 KasI G'GCGC_C Cuts at: 2056 2056 Size: 5149 KpnI G_GTAC'C Cuts at: 2474 2474 Size: 5149 MfeI C'AATT_G Cuts at: 2748 2748 Size: 5149 MluI A'CGCG_T Cuts at: 5025 5025 Size: 5149 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4263 4447 1305 Size: 2958 184 2007 Fragments arranged by size: 2958 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3327 Fragments arranged by size: 3327 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3107 3466 3625 518 Size: 648 1941 359 159 2042 Fragments arranged by size: 2042 1941 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2026 2080 2215 2437 2565 3242 4390 4635 Size: 420 54 135 222 128 677 1148 245 Cuts at: 4635 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 420 245 222 135 128 54 NarI GG'CG_CC Cuts at: 2057 2057 Size: 5149 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4597 NdeI CA'TA_TG Cuts at: 387 387 Size: 5149 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5149 NotI GC'GGCC_GC Cuts at: 2596 2596 Size: 5149 NspI r_CATG'y Cuts at: 1132 1229 2507 4052 1132 Size: 97 1278 1545 2229 Fragments arranged by size: 2229 1545 1278 97 PasI CC'CwG_GG Cuts at: 1348 2314 1348 Size: 966 4183 PciI A'CATG_T Cuts at: 4048 4048 Size: 5149 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5149 PmeI GTTT'AAAC Cuts at: 2453 2453 Size: 5149 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3218 3250 4731 4763 3218 Size: 32 1481 32 3604 Fragments arranged by size: 3604 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5149 PsiI TTA'TAA Cuts at: 2719 2719 Size: 5149 PstI C_TGCA'G Cuts at: 830 2501 830 Size: 1671 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5149 PvuII CAG'CTG Cuts at: 2215 2437 2215 Size: 222 4927 SacI G_AGCT'C Cuts at: 2022 2022 Size: 5149 SalI G'TCGA_C Cuts at: 1936 2491 1936 Size: 555 4594 SbfI CC_TGCA'GG Cuts at: 2501 2501 Size: 5149 ScaI AGT'ACT Cuts at: 3397 5109 3397 Size: 1712 3437 SfcI C'TryA_G Cuts at: 826 1045 2497 3632 4313 4504 826 Size: 219 1452 1135 681 191 1471 Fragments arranged by size: 1471 1452 1135 681 219 191 SfoI GGC'GCC Cuts at: 2058 2058 Size: 5149 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5149 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5149 SmaI CCC'GGG Cuts at: 1872 2063 2476 1872 Size: 191 413 4545 Fragments arranged by size: 4545 413 191 SmlI C'TyrA_G Cuts at: 820 1017 1943 3253 4154 4416 820 Size: 197 926 1310 901 262 1553 Fragments arranged by size: 1553 1310 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2940 493 Size: 2447 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5149 SphI G_CATG'C Cuts at: 1132 2507 1132 Size: 1375 3774 SspI AAT'ATT Cuts at: 5 52 3073 5 Size: 47 3021 2081 Fragments arranged by size: 3021 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5149 StyI C'CwwG_G Cuts at: 513 1065 2307 2588 513 Size: 552 1242 281 3074 Fragments arranged by size: 3074 1242 552 281 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3176 3193 3346 3531 3976 325 Size: 1255 1596 17 153 185 445 1498 Fragments arranged by size: 1596 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2223 3395 5107 Size: 274 80 33 51 1443 246 1172 1712 Cuts at: 5107 96 Size: 138 Fragments arranged by size: 1712 1443 1172 274 246 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2085 2117 5109 5141 2085 Size: 32 2992 32 2093 Fragments arranged by size: 2992 2093 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5149 XbaI T'CTAG_A Cuts at: 2485 2485 Size: 5149 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5149 XmaI C'CCGG_G Cuts at: 1870 2061 2474 1870 Size: 191 413 4545 Fragments arranged by size: 4545 413 191 XmnI GAAnn'nnTTC Cuts at: 3278 3278 Size: 5149 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2957 276 Size: 53 83 186 936 1423 2468 Fragments arranged by size: 2468 1423 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI