Restriction map for
pFN21AB6757
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# NO CUT ENZYMES ( 194 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BanI BanII BbeI BbrPI BbvCI
BbvI BcgI BciVI BclI BcnI
BfaI BglI BglII BlnI BlpI
BmyI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
Fnu4HI FokI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HpaII
ItaI KasI KpnI MaeI MaeII
MaeIII MamI MfeI MluI MluNI
MroI MscI MslI MspI MunI
MwoI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsaI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SwaI TfiI TseI Tsp45I
Tsp509I TspRI Tth111I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccII 12 CGCG CG^CG
AflII 301 CTTAAG C^TTAAG
AluI 152 AGCT AG^CT
AvaII 247 GGACC G^G[AT]CC
BamHI 144 GGATCC G^GATCC
BbsI 95 GTCTTC GAAGAC(2/6)
BfrI 301 CTTAAG C^TTAAG
BpuAI 95 GTCTTC GAAGAC(2/6)
BseRI 274 CTCCTC GAGGAG(10/8)
BsiYI 251 CCAGGCCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 251 CCAGGCCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 58 GTCCC GGGAC(10/14)
BsrI 230 ACTGG ACTGG(1/-1)
BssKI 251 CCAGG ^CC[ACGT]GG
BstNI 251 CCAGG CC^[AT]GG
BstUI 12 CGCG CG^CG
Cac8I 21 GCGTGC GC[ACGT]^[ACGT]GC
Cfr13I 247 GGACC G^G[ACGT]CC
DraII 246 AGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 11 CCGCGG C^C[GA][CT]GG
EarI 132 GAAGAG CTCTTC(1/4)
EcoO109I 246 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRII 251 CCAGG ^CC[AT]GG
FauI 10 CCCGC CCCGC(4/6)
HaeIII 254 GGCC GG^CC
HphI 15 GGTGA GGTGA(8/7)
Ksp632I 132 GAAGAG CTCTTC(1/4)
KspI 11 CCGCGG CCGC^GG
MseI 302 TTAA T^TAA
MspA1I 11 CCGCGG C[AC]G^C[GT]G
MvaI 251 CCAGG CC^[AT]GG
MvnI 12 CGCG CG^CG
NlaIII 63 CATG CATG^
PflMI 251 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 246 AGGACCC [GA]G^G[AT]CC[CT]
SacII 11 CCGCGG CCGC^GG
Sau96I 247 GGACC G^G[ACGT]CC
ScrFI 251 CCAGG CC^[ACGT]GG
SmlI 301 CTTAAG C^T[CT][GA]AG
SspI 238 AATATT AAT^ATT
StuI 253 AGGCCT AGG^CCT
TaqI 243 TCGA T^CGA
Tru9I 302 TTAA T^TAA
TthHB8I 243 TCGA T^CGA
Van91I 251 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AciI 11 CCGC CCGC(-3/-1)
13 GCGG
BstYI 144 GGATCC [GA]^GATC[CT]
264 AGATCC
DdeI 149 CTGAG C^T[ACGT]AG
308 CTAAG
DpnII 145 GATC ^GATC
265 GATC
EcoT14I 256 CCTTGG C^C[AT][AT]GG
279 CCAAGG
MboI 145 GATC ^GATC
265 GATC
MflI 144 GGATCC [GA]^GATC[CT]
264 AGATCC
NdeII 145 GATC ^GATC
265 GATC
NlaIV 144 GGATCC GG[ACGT]^[ACGT]CC
247 GGACCC
Sau3AI 145 GATC ^GATC
265 GATC
SfaNI 18 GATGC GCATC(5/9)
124 GATGC
StyI 256 CCTTGG C^C[AT][AT]GG
279 CCAAGG
XhoII 144 GGATCC [GA]^GATC[CT]
264 AGATCC
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# 3 OR MORE CUT ENZYMES ( 6 enzymes) #
#########################################
AlwI BsaJI HinfI MboII MnlI
PleI