(Circular) (Six-base) MAPSORT of: pFN21AB6575.seq Check: 5726 from: 1 to: 5833 pFN21AB6575 5833 bp With 182 enzymes: SgfI * October 20, 2009 10:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2450 2450 Size: 5833 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3643 278 Size: 53 83 186 936 2107 2468 Fragments arranged by size: 2468 2107 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2514 3176 1937 Size: 577 662 4594 Fragments arranged by size: 4594 662 577 Acc65I G'GTAC_C Cuts at: 3154 3154 Size: 5833 AclI AA'CG_TT Cuts at: 1563 3960 4333 1563 Size: 2397 373 3063 Fragments arranged by size: 3063 2397 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2528 3896 5280 5548 1632 Size: 896 1368 1384 268 1917 Fragments arranged by size: 1917 1384 1368 896 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5636 AflIII A'CryG_T Cuts at: 1203 4732 5709 1203 Size: 3529 977 1327 Fragments arranged by size: 3529 1327 977 AgeI A'CCGG_T Cuts at: 4664 4664 Size: 5833 AhdI GACnn_n'nnGTC Cuts at: 4562 4562 Size: 5833 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5833 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5833 AlwNI CAG_nnn'CTG Cuts at: 3576 5148 3576 Size: 1572 4261 ApaLI G'TGCA_C Cuts at: 2379 3890 5046 2379 Size: 1511 1156 3166 Fragments arranged by size: 3166 1511 1156 ApoI r'AATT_y Cuts at: 1493 2370 3142 3368 3520 5629 1493 Size: 877 772 226 152 2109 1697 Fragments arranged by size: 2109 1697 877 772 226 152 AseI AT'TA_AT Cuts at: 160 1032 4387 160 Size: 872 3355 1606 Fragments arranged by size: 3355 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5833 AvaI C'yCGr_G Cuts at: 1870 1943 3158 1870 Size: 73 1215 4545 Fragments arranged by size: 4545 1215 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3039 3072 3039 Size: 33 5800 BamHI G'GATC_C Cuts at: 3163 3530 3163 Size: 367 5466 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3154 4609 618 Size: 325 217 627 1367 1455 1842 Fragments arranged by size: 1842 1455 1367 627 325 217 BanII G_rGCy'C Cuts at: 1391 3152 1391 Size: 1761 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2021 928 Size: 971 122 4740 Fragments arranged by size: 4740 971 122 BbvCI CC'TCA_GC Cuts at: 2109 2109 Size: 5833 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4024 4058 1903 Size: 34 50 34 2003 34 3678 Fragments arranged by size: 3678 2003 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3725 4935 3725 Size: 1210 4623 BclI T'GATC_A Cuts at: 1548 2811 1548 Size: 1263 4570 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4444 136 Size: 107 122 71 631 605 2772 1525 Fragments arranged by size: 2772 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5828 5828 Size: 5833 BlpI GC'TnA_GC Cuts at: 2141 3250 2141 Size: 1109 4724 Bme1580I G_kGCm'C Cuts at: 1790 2352 2383 3894 5050 1790 Size: 562 31 1511 1156 2573 Fragments arranged by size: 2573 1511 1156 562 31 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5833 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3564 4522 442 Size: 416 53 83 399 24 2147 958 1753 Fragments arranged by size: 2147 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5833 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2225 2225 Size: 5833 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2049 4493 1239 Size: 810 2444 2579 Fragments arranged by size: 2579 2444 810 Bpu10I CC'TnA_GC Cuts at: 2109 2109 Size: 5833 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2297 3112 3958 4823 5121 2297 Size: 815 846 865 298 3009 Fragments arranged by size: 3009 865 846 815 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3108 4496 882 Size: 617 1609 1388 2219 Fragments arranged by size: 2219 1609 1388 617 BsaAI yAC'GTr Cuts at: 493 1204 3624 493 Size: 711 2420 2702 Fragments arranged by size: 2702 2420 711 BsaBI GATnn'nnATC Cuts at: 2880 5827 2880 Size: 2947 2886 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3640 4022 4657 275 Size: 53 83 186 936 2107 382 635 1451 Fragments arranged by size: 2107 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4266 4664 4938 5085 5496 4266 Size: 398 274 147 411 4603 Fragments arranged by size: 4603 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5608 Fragments arranged by size: 5608 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2466 1186 Size: 1280 4553 BseYI C'CCAG_C Cuts at: 2802 2993 5036 5532 2802 Size: 191 2043 496 3103 Fragments arranged by size: 3103 2043 496 191 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2450 1163 Size: 573 714 4546 Fragments arranged by size: 4546 714 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3283 4044 5072 1540 Size: 275 186 1282 761 1028 2301 Fragments arranged by size: 2301 1282 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2383 3098 3894 3979 5050 2383 Size: 715 796 85 1071 3166 Fragments arranged by size: 3166 1071 796 715 85 BsmI GAATG_Cn' Cuts at: 2532 3011 3344 3437 2532 Size: 479 333 93 4928 Fragments arranged by size: 4928 479 333 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2352 2383 3098 3152 3894 3979 5050 Size: 399 562 31 715 54 742 85 1071 Cuts at: 5050 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 715 562 399 85 54 31 BspEI T'CCGG_A Cuts at: 5496 5496 Size: 5833 BspHI T'CATG_A Cuts at: 2752 3722 5452 2752 Size: 970 1730 3133 Fragments arranged by size: 3133 1730 970 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2450 3188 844 Size: 1606 738 3489 Fragments arranged by size: 3489 1606 738 BsrBI CCG'CTC Cuts at: 1394 1823 3720 1394 Size: 429 1897 3507 Fragments arranged by size: 3507 1897 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3071 4328 4502 65 Size: 1183 1823 1257 174 1396 Fragments arranged by size: 1823 1396 1257 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4477 4664 1700 Size: 110 2667 187 2869 Fragments arranged by size: 2869 2667 187 110 BsrGI T'GTAC_A Cuts at: 96 2258 2982 96 Size: 2162 724 2947 Fragments arranged by size: 2947 2162 724 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5833 BssSI C'ACGA_G Cuts at: 3111 3893 4905 3111 Size: 782 1012 4039 Fragments arranged by size: 4039 1012 782 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5833 BstBI TT'CG_AA Cuts at: 4637 4637 Size: 5833 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5833 BstYI r'GATC_y Cuts at: 1972 3163 3530 3915 3932 5384 5470 5482 5828 Size: 1191 367 385 17 1452 86 12 346 Cuts at: 5828 1972 Size: 1977 Fragments arranged by size: 1977 1452 1191 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3033 3033 Size: 5833 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5281 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5094 Fragments arranged by size: 5094 683 56 BtsI GCAGTG_nn' Cuts at: 3349 4143 4163 3349 Size: 794 20 5019 Fragments arranged by size: 5019 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5833 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5798 DraI TTT'AAA Cuts at: 3137 3137 Size: 5833 DraIII CAC_nnn'GTG Cuts at: 2387 2387 Size: 5833 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4840 809 Size: 507 3524 1802 Fragments arranged by size: 3524 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3280 4169 5798 Size: 54 1425 50 275 18 1450 889 1629 Cuts at: 5798 8 Size: 43 Fragments arranged by size: 1629 1450 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3280 1537 Size: 275 1468 4090 Fragments arranged by size: 4090 1468 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2194 2778 3763 1353 Size: 841 584 985 3423 Fragments arranged by size: 3423 985 841 584 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4420 4794 4940 387 Size: 1064 138 2831 374 146 1280 Fragments arranged by size: 2831 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2049 2528 3896 4493 5280 5548 1239 Size: 393 417 479 1368 597 787 268 1524 Fragments arranged by size: 1524 1368 787 597 479 417 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2904 2904 Size: 5833 EcoRI G'AATT_C Cuts at: 3142 3520 3142 Size: 378 5455 EcoRV GAT'ATC Cuts at: 2160 2160 Size: 5833 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2693 2693 Size: 5833 FspI TGC'GCA Cuts at: 4339 4339 Size: 5833 HaeII r_GCGC'y Cuts at: 1678 4980 1678 Size: 3302 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2753 2785 4474 4506 4548 Size: 32 135 32 1181 32 1689 32 42 Cuts at: 4548 4580 1373 Size: 32 2626 Fragments arranged by size: 2626 1689 1181 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3177 3423 669 Size: 988 281 1239 246 3079 Fragments arranged by size: 3079 1239 988 281 246 HindIII A'AGCT_T Cuts at: 748 2586 748 Size: 1838 3995 HpaI GTT'AAC Cuts at: 3423 3423 Size: 5833 KpnI G_GTAC'C Cuts at: 3158 3158 Size: 5833 MfeI C'AATT_G Cuts at: 3432 3432 Size: 5833 MluI A'CGCG_T Cuts at: 5709 5709 Size: 5833 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2371 4947 5131 1305 Size: 1066 2576 184 2007 Fragments arranged by size: 2576 2007 1066 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4011 Fragments arranged by size: 4011 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2404 3791 4150 4309 518 Size: 648 1238 1387 359 159 2042 Fragments arranged by size: 2042 1387 1238 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2038 2059 2069 3249 3926 5074 5319 1606 Size: 432 21 10 1180 677 1148 245 2120 Fragments arranged by size: 2120 1180 1148 677 432 245 21 10 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5281 NdeI CA'TA_TG Cuts at: 387 387 Size: 5833 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5833 NotI GC'GGCC_GC Cuts at: 3280 3280 Size: 5833 NspI r_CATG'y Cuts at: 1132 1229 2927 3069 3191 4736 1132 Size: 97 1698 142 122 1545 2229 Fragments arranged by size: 2229 1698 1545 142 122 97 PasI CC'CwG_GG Cuts at: 1348 2293 1348 Size: 945 4888 PciI A'CATG_T Cuts at: 4732 4732 Size: 5833 PflMI CCAn_nnn'nTGG Cuts at: 1108 2390 1108 Size: 1282 4551 PmeI GTTT'AAAC Cuts at: 3137 3137 Size: 5833 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3902 3934 5415 5447 3902 Size: 32 1481 32 4288 Fragments arranged by size: 4288 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5833 PsiI TTA'TAA Cuts at: 3403 3403 Size: 5833 PstI C_TGCA'G Cuts at: 830 3185 830 Size: 2355 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5833 SalI G'TCGA_C Cuts at: 1936 3175 1936 Size: 1239 4594 SbfI CC_TGCA'GG Cuts at: 3185 3185 Size: 5833 ScaI AGT'ACT Cuts at: 3015 4081 5793 3015 Size: 1066 1712 3055 Fragments arranged by size: 3055 1712 1066 SexAI A'CCwGG_T Cuts at: 2383 2383 Size: 5833 SfcI C'TryA_G Cuts at: 826 1045 3181 4316 4997 5188 826 Size: 219 2136 1135 681 191 1471 Fragments arranged by size: 2136 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5833 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5833 SmaI CCC'GGG Cuts at: 1872 3160 1872 Size: 1288 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2276 3091 3937 4838 5100 820 Size: 197 926 333 815 846 901 262 1553 Fragments arranged by size: 1553 926 901 846 815 333 262 197 SnaBI TAC'GTA Cuts at: 493 3624 493 Size: 3131 2702 SpeI A'CTAG_T Cuts at: 152 2716 152 Size: 2564 3269 SphI G_CATG'C Cuts at: 1132 3191 1132 Size: 2059 3774 SspI AAT'ATT Cuts at: 5 52 2614 3757 5 Size: 47 2562 1143 2081 Fragments arranged by size: 2562 2081 1143 47 StuI AGG'CCT Cuts at: 1517 2888 1517 Size: 1371 4462 StyI C'CwwG_G Cuts at: 513 1065 3272 513 Size: 552 2207 3074 Fragments arranged by size: 3074 2207 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2973 3007 3860 3877 4030 4215 4660 Size: 1255 1393 34 853 17 153 185 445 Cuts at: 4660 325 Size: 1498 Fragments arranged by size: 1498 1393 1255 853 445 185 153 34 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2258 2982 3013 Size: 274 80 33 51 1443 281 724 31 Cuts at: 3013 4079 5791 96 Size: 1066 1712 138 Fragments arranged by size: 1712 1443 1066 724 281 274 138 80 51 33 31 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5793 5825 5793 Size: 32 5801 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5833 XbaI T'CTAG_A Cuts at: 3169 3169 Size: 5833 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5833 XmaI C'CCGG_G Cuts at: 1870 3158 1870 Size: 1288 4545 XmnI GAAnn'nnTTC Cuts at: 2018 3962 2018 Size: 1944 3889 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3641 276 Size: 53 83 186 936 2107 2468 Fragments arranged by size: 2468 2107 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BfrBI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI