Restriction map for
pFN21AB6567
(Circular) (Six-base) MAPSORT of: pFN21AB6567.seq Check: 5913 from: 1 to: 5827
pFN21AB6567 5827 bp
With 182 enzymes: SgfI *
October 20, 2009 10:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3637 278
Size: 53 83 186 936 2101 2468
Fragments arranged by size:
2468 2101 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3170 1937
Size: 1233 4594
Acc65I G'GTAC_C
Cuts at: 3148 3148
Size: 5827
AclI AA'CG_TT
Cuts at: 1563 3954 4327 1563
Size: 2391 373 3063
Fragments arranged by size:
3063 2391 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2391 2400 2445 3890 5274 5542 1632
Size: 759 9 45 1445 1384 268 1917
Fragments arranged by size:
1917 1445 1384 759 268 45 9
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5630
AflIII A'CryG_T
Cuts at: 1203 2294 4726 5703 1203
Size: 1091 2432 977 1327
Fragments arranged by size:
2432 1327 1091 977
AgeI A'CCGG_T
Cuts at: 4658 4658
Size: 5827
AhdI GACnn_n'nnGTC
Cuts at: 4556 4556
Size: 5827
AleI CACnn'nnGTG
Cuts at: 1166 3002 1166
Size: 1836 3991
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5827
AlwNI CAG_nnn'CTG
Cuts at: 2650 2987 2999 3570 5142 2650
Size: 337 12 571 1572 3335
Fragments arranged by size:
3335 1572 571 337 12
ApaLI G'TGCA_C
Cuts at: 3884 5040 3884
Size: 1156 4671
ApoI r'AATT_y
Cuts at: 1493 2750 2767 3013 3136 3362 3514 5623 1493
Size: 1257 17 246 123 226 152 2109 1697
Fragments arranged by size:
2109 1697 1257 246 226 152 123 17
AseI AT'TA_AT
Cuts at: 160 1032 4381 160
Size: 872 3349 1606
Fragments arranged by size:
3349 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5827
AvaI C'yCGr_G
Cuts at: 1870 1943 3152 1870
Size: 73 1209 4545
Fragments arranged by size:
4545 1209 73
BamHI G'GATC_C
Cuts at: 3157 3524 3157
Size: 367 5460
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3078 3148 4603 618
Size: 325 217 627 1291 70 1455 1842
Fragments arranged by size:
1842 1455 1291 627 325 217 70
BanII G_rGCy'C
Cuts at: 1391 3146 1391
Size: 1755 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2122 2379 2426 928
Size: 971 223 257 47 4329
Fragments arranged by size:
4329 971 257 223 47
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4018 4052 1903
Size: 34 2081 34 3678
Fragments arranged by size:
3678 2081 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3719 4929 3719
Size: 1210 4617
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5827
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4438 136
Size: 107 122 71 631 605 2766 1525
Fragments arranged by size:
2766 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5822 5822
Size: 5827
BlpI GC'TnA_GC
Cuts at: 2678 3244 2678
Size: 566 5261
Bme1580I G_kGCm'C
Cuts at: 1790 3888 5044 1790
Size: 2098 1156 2573
Fragments arranged by size:
2573 2098 1156
BmgBI CAC'GTC
Cuts at: 1318 2295 1318
Size: 977 4850
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2416 3558 4516
Size: 416 53 83 399 24 999 1142 958
Cuts at: 4516 442
Size: 1753
Fragments arranged by size:
1753 1142 999 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5827
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2092 2092
Size: 5827
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2372 2672 2843 4487 1239
Size: 1133 300 171 1644 2579
Fragments arranged by size:
2579 1644 1133 300 171
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2062 3952 4817 5115 2062
Size: 1890 865 298 2774
Fragments arranged by size:
2774 1890 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4490 882
Size: 617 2991 2219
Fragments arranged by size:
2991 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3618 493
Size: 711 2414 2702
Fragments arranged by size:
2702 2414 711
BsaBI GATnn'nnATC
Cuts at: 5821 5821
Size: 5827
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3634 4016 4651 275
Size: 53 83 186 936 2101 382 635 1451
Fragments arranged by size:
2101 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3124 4260 4658 4932 5079 5490 3124
Size: 1136 398 274 147 411 3461
Fragments arranged by size:
3461 1136 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2345 2375 1178
Size: 30 165 30 942 30 4630
Fragments arranged by size:
4630 942 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2101 2369 2687 2840 1186
Size: 915 268 318 153 4173
Fragments arranged by size:
4173 915 318 268 153
BseYI C'CCAG_C
Cuts at: 2453 5030 5526 2453
Size: 2577 496 2754
Fragments arranged by size:
2754 2577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2407 2606 3043 3069 1163
Size: 573 671 199 437 26 3921
Fragments arranged by size:
3921 671 573 437 199 26
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3277 4038 5066 1540
Size: 275 186 1276 761 1028 2301
Fragments arranged by size:
2301 1276 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3888 3973 5044 3888
Size: 85 1071 4671
Fragments arranged by size:
4671 1071 85
BsmI GAATG_Cn'
Cuts at: 2497 2556 3338 3431 2497
Size: 59 782 93 4893
Fragments arranged by size:
4893 782 93 59
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3146 3888 3973 5044 1391
Size: 399 1356 742 85 1071 2174
Fragments arranged by size:
2174 1356 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5490 5490
Size: 5827
BspHI T'CATG_A
Cuts at: 3716 5446 3716
Size: 1730 4097
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3102 3182 844
Size: 2258 80 3489
Fragments arranged by size:
3489 2258 80
BsrBI CCG'CTC
Cuts at: 1394 1823 3714 1394
Size: 429 1891 3507
Fragments arranged by size:
3507 1891 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4322 4496 65
Size: 1183 3074 174 1396
Fragments arranged by size:
3074 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4471 4658 1700
Size: 110 2661 187 2869
Fragments arranged by size:
2869 2661 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5827
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5827
BssSI C'ACGA_G
Cuts at: 3887 4899 3887
Size: 1012 4815
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2999 794
Size: 2205 3622
BstBI TT'CG_AA
Cuts at: 2854 4631 2854
Size: 1777 4050
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5827
BstXI CCAn_nnnn'nTGG
Cuts at: 3004 3004
Size: 5827
BstYI r'GATC_y
Cuts at: 1972 3157 3524 3909 3926 5378 5464 5476 5822
Size: 1185 367 385 17 1452 86 12 346
Cuts at: 5822 1972
Size: 1977
Fragments arranged by size:
1977 1452 1185 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2124 2721 513
Size: 552 939 120 597 3619
Fragments arranged by size:
3619 939 597 552 120
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5088
Fragments arranged by size:
5088 683 56
BtsI GCAGTG_nn'
Cuts at: 2151 2997 3343 4137 4157 2151
Size: 846 346 794 20 3821
Fragments arranged by size:
3821 846 794 346 20
ClaI AT'CG_AT
Cuts at: 1554 2871 1554
Size: 1317 4510
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5792
DraI TTT'AAA
Cuts at: 2053 2317 2560 2614 3131 2053
Size: 264 243 54 517 4749
Fragments arranged by size:
4749 517 264 243 54
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4834 809
Size: 507 3518 1802
Fragments arranged by size:
3518 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2121 3274 4163
Size: 54 1425 50 275 18 291 1153 889
Cuts at: 4163 5792 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1153 889 291 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3274 1537
Size: 275 1462 4090
Fragments arranged by size:
4090 1462 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2061 2376 2664 3124 3757 1353
Size: 708 315 288 460 633 3423
Fragments arranged by size:
3423 708 633 460 315 288
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4414 4788 4934 387
Size: 1064 138 2825 374 146 1280
Fragments arranged by size:
2825 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2372 2391 2400 2445 2672 2843 3890
Size: 393 740 19 9 45 227 171 1047
Cuts at: 3890 4487 5274 5542 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1047 787 740 597 393 268 227
171 45 19 9
EcoO109I rG'GnC_Cy
Cuts at: 2647 2647
Size: 5827
EcoRI G'AATT_C
Cuts at: 3013 3136 3514 3013
Size: 123 378 5326
Fragments arranged by size:
5326 378 123
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2822 2914 2822
Size: 92 5735
FspI TGC'GCA
Cuts at: 4333 4333
Size: 5827
HaeII r_GCGC'y
Cuts at: 1678 4974 1678
Size: 3296 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2345 2377 2449 2481 4468
Size: 32 135 32 773 32 72 32 1987
Cuts at: 4468 4500 4542 4574 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1987 773 135 72 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2236 2781 2913 3171 3417 669
Size: 988 281 298 545 132 258 246 3079
Fragments arranged by size:
3079 988 545 298 281 258 246 132
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5827
HpaI GTT'AAC
Cuts at: 2236 3417 2236
Size: 1181 4646
KpnI G_GTAC'C
Cuts at: 3152 3152
Size: 5827
MfeI C'AATT_G
Cuts at: 3426 3426
Size: 5827
MluI A'CGCG_T
Cuts at: 5703 5703
Size: 5827
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4941 5125 1305
Size: 3636 184 2007
Fragments arranged by size:
3636 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2123 10
Size: 54 1425 343 291 3714
Fragments arranged by size:
3714 1425 343 291 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2400 3002 3785 4144 4303 518
Size: 648 1234 602 783 359 159 2042
Fragments arranged by size:
2042 1234 783 648 602 359 159
MspA1I CmG'CkG
Cuts at: 1606 3243 3920 5068 5313 1606
Size: 1637 677 1148 245 2120
Fragments arranged by size:
2120 1637 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2124 513
Size: 552 939 120 4216
Fragments arranged by size:
4216 939 552 120
NdeI CA'TA_TG
Cuts at: 387 2743 387
Size: 2356 3471
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5827
NotI GC'GGCC_GC
Cuts at: 3274 3274
Size: 5827
NspI r_CATG'y
Cuts at: 1132 1229 3185 4730 1132
Size: 97 1956 1545 2229
Fragments arranged by size:
2229 1956 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5827
PciI A'CATG_T
Cuts at: 4726 4726
Size: 5827
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2650 1108
Size: 1542 4285
PfoI T'CCnGG_A
Cuts at: 2649 2649
Size: 5827
PmeI GTTT'AAAC
Cuts at: 3131 3131
Size: 5827
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2233 2265 3896 3928 5409 5441 2233
Size: 32 1631 32 1481 32 2619
Fragments arranged by size:
2619 1631 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2647 2647
Size: 5827
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5827
PsiI TTA'TAA
Cuts at: 3397 3397
Size: 5827
PstI C_TGCA'G
Cuts at: 830 2158 3179 830
Size: 1328 1021 3478
Fragments arranged by size:
3478 1328 1021
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5827
SalI G'TCGA_C
Cuts at: 1936 3169 1936
Size: 1233 4594
SbfI CC_TGCA'GG
Cuts at: 3179 3179
Size: 5827
ScaI AGT'ACT
Cuts at: 2255 4075 5787 2255
Size: 1820 1712 2295
Fragments arranged by size:
2295 1820 1712
SfcI C'TryA_G
Cuts at: 826 1045 2154 3175 4310 4991 5182 826
Size: 219 1109 1021 1135 681 191 1471
Fragments arranged by size:
1471 1135 1109 1021 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5827
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5827
SmaI CCC'GGG
Cuts at: 1872 3154 1872
Size: 1282 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2077 3931 4832 5094 820
Size: 197 926 134 1854 901 262 1553
Fragments arranged by size:
1854 1553 926 901 262 197 134
SnaBI TAC'GTA
Cuts at: 493 3618 493
Size: 3125 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5827
SphI G_CATG'C
Cuts at: 1132 3185 1132
Size: 2053 3774
SspI AAT'ATT
Cuts at: 5 52 2058 3751 5
Size: 47 2006 1693 2081
Fragments arranged by size:
2081 2006 1693 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5827
StyI C'CwwG_G
Cuts at: 513 1065 2004 2124 2889 2937 3266 513
Size: 552 939 120 765 48 329 3074
Fragments arranged by size:
3074 939 765 552 329 120 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2398 2657 3854 3871 4024 4209 4654
Size: 1255 818 259 1197 17 153 185 445
Cuts at: 4654 325
Size: 1498
Fragments arranged by size:
1498 1255 1197 818 445 259 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2253 2538 4073
Size: 274 80 33 51 1443 276 285 1535
Cuts at: 4073 5785 96
Size: 1712 138
Fragments arranged by size:
1712 1535 1443 285 276 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5787 5819 5787
Size: 32 5795
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5827
XbaI T'CTAG_A
Cuts at: 3163 3163
Size: 5827
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2131 2818 2131
Size: 687 5140
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5827
XmaI C'CCGG_G
Cuts at: 1870 3152 1870
Size: 1282 4545
XmnI GAAnn'nnTTC
Cuts at: 3956 3956
Size: 5827
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3635 276
Size: 53 83 186 936 2101 2468
Fragments arranged by size:
2468 2101 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI