(Circular) (Six-base) MAPSORT of: pFN21AB5573.seq Check: 1178 from: 1 to: 8320 pFN21AB5573 8320 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2227 3583 2227 Size: 1356 6964 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6130 278 Size: 53 83 186 936 4594 2468 Fragments arranged by size: 4594 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2173 5663 1937 Size: 236 3490 4594 Fragments arranged by size: 4594 3490 236 Acc65I G'GTAC_C Cuts at: 5641 5641 Size: 8320 AclI AA'CG_TT Cuts at: 1563 6447 6820 1563 Size: 4884 373 3063 Fragments arranged by size: 4884 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2720 3662 4403 4428 5044 5186 5195 6383 Size: 1088 942 741 25 616 142 9 1188 Cuts at: 6383 7767 8035 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1188 1088 942 741 616 268 142 25 9 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8123 AflIII A'CryG_T Cuts at: 1203 7219 8196 1203 Size: 6016 977 1327 Fragments arranged by size: 6016 1327 977 AgeI A'CCGG_T Cuts at: 7151 7151 Size: 8320 AhdI GACnn_n'nnGTC Cuts at: 4508 7049 4508 Size: 2541 5779 AleI CACnn'nnGTG Cuts at: 1166 4295 4904 1166 Size: 3129 609 4582 Fragments arranged by size: 4582 3129 609 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3372 5585 1184 Size: 2188 2213 3919 Fragments arranged by size: 3919 2213 2188 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2555 2587 2555 Size: 32 8288 AlwNI CAG_nnn'CTG Cuts at: 5092 6063 7635 5092 Size: 971 1572 5777 Fragments arranged by size: 5777 1572 971 ApaLI G'TGCA_C Cuts at: 6377 7533 6377 Size: 1156 7164 ApoI r'AATT_y Cuts at: 1493 4181 4823 5042 5408 5629 5855 6007 8116 Size: 2688 642 219 366 221 226 152 2109 Cuts at: 8116 1493 Size: 1697 Fragments arranged by size: 2688 2109 1697 642 366 226 221 219 152 AseI AT'TA_AT Cuts at: 160 1032 2136 6874 160 Size: 872 1104 4738 1606 Fragments arranged by size: 4738 1606 1104 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8320 AvaI C'yCGr_G Cuts at: 1870 1943 3677 4113 5645 1870 Size: 73 1734 436 1532 4545 Fragments arranged by size: 4545 1734 1532 436 73 BamHI G'GATC_C Cuts at: 5650 6017 5650 Size: 367 7953 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3034 3779 4036 4710 4852 Size: 325 217 627 1247 745 257 674 142 Cuts at: 4852 5192 5641 7096 618 Size: 340 449 1455 1842 Fragments arranged by size: 1842 1455 1247 745 674 627 449 340 325 257 217 142 BanII G_rGCy'C Cuts at: 1391 2717 3891 3954 4686 5639 1391 Size: 1326 1174 63 732 953 4072 Fragments arranged by size: 4072 1326 1174 953 732 63 BbeI G_GCGC'C Cuts at: 3783 4714 3783 Size: 931 7389 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3481 928 Size: 971 1582 5767 Fragments arranged by size: 5767 1582 971 BbvCI CC'TCA_GC Cuts at: 5075 5075 Size: 8320 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3865 3899 6511 6545 1903 Size: 34 1928 34 2612 34 3678 Fragments arranged by size: 3678 2612 1928 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5312 6212 7422 5312 Size: 900 1210 6210 Fragments arranged by size: 6210 1210 900 BclI T'GATC_A Cuts at: 1548 2481 4644 1548 Size: 933 2163 5224 Fragments arranged by size: 5224 2163 933 BfrBI ATG'CAT Cuts at: 4775 4775 Size: 8320 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3274 4719 6931 Size: 107 122 71 631 605 1602 1445 2212 Cuts at: 6931 136 Size: 1525 Fragments arranged by size: 2212 1602 1525 1445 631 605 122 107 71 BglII A'GATC_T Cuts at: 2218 3637 4239 8315 2218 Size: 1419 602 4076 2223 Fragments arranged by size: 4076 2223 1419 602 BlpI GC'TnA_GC Cuts at: 5737 5737 Size: 8320 Bme1580I G_kGCm'C Cuts at: 1790 3678 5015 6381 7537 1790 Size: 1888 1337 1366 1156 2573 Fragments arranged by size: 2573 1888 1366 1337 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8320 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5575 6051 7009 Size: 416 53 83 399 24 4158 476 958 Cuts at: 7009 442 Size: 1753 Fragments arranged by size: 4158 1753 958 476 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8320 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2243 2351 3549 4605 6980 1239 Size: 1004 108 1198 1056 2375 2579 Fragments arranged by size: 2579 2375 1198 1056 1004 108 Bpu10I CC'TnA_GC Cuts at: 4248 5075 4248 Size: 827 7493 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6445 7310 7608 6445 Size: 865 298 7157 Fragments arranged by size: 7157 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3359 6983 882 Size: 617 1860 3624 2219 Fragments arranged by size: 3624 2219 1860 617 BsaAI yAC'GTr Cuts at: 493 1204 6111 493 Size: 711 4907 2702 Fragments arranged by size: 4907 2702 711 BsaBI GATnn'nnATC Cuts at: 5033 8314 5033 Size: 3281 5039 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3780 4711 6127 6509 Size: 53 83 186 936 2247 931 1416 382 Cuts at: 6509 7144 275 Size: 635 1451 Fragments arranged by size: 2247 1451 1416 936 931 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3822 3909 6753 7151 7425 7572 7983 3822 Size: 87 2844 398 274 147 411 4159 Fragments arranged by size: 4159 2844 411 398 274 147 87 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2555 2585 3171 3201 4508 Size: 30 165 30 1152 30 586 30 1307 Cuts at: 4508 4538 1178 Size: 30 4960 Fragments arranged by size: 4960 1307 1152 586 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3695 5219 5435 5438 5474 1186 Size: 2509 1524 216 3 36 4032 Fragments arranged by size: 4032 2509 1524 216 36 3 BseYI C'CCAG_C Cuts at: 4871 5183 7523 8019 4871 Size: 312 2340 496 5172 Fragments arranged by size: 5172 2340 496 312 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2059 4643 5591 1163 Size: 573 323 2584 948 3892 Fragments arranged by size: 3892 2584 948 573 323 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5770 6531 7559 1540 Size: 275 186 3769 761 1028 2301 Fragments arranged by size: 3769 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2717 3619 3734 5505 6381 6466 7537 2717 Size: 902 115 1771 876 85 1071 3500 Fragments arranged by size: 3500 1771 1071 902 876 115 85 BsmI GAATG_Cn' Cuts at: 4137 4775 5355 5831 5924 4137 Size: 638 580 476 93 6533 Fragments arranged by size: 6533 638 580 476 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2717 3619 3678 3734 3891 3954 4686 Size: 399 927 902 59 56 157 63 732 Cuts at: 4686 5015 5505 5639 6381 6466 7537 1391 Size: 329 490 134 742 85 1071 2174 Fragments arranged by size: 2174 1071 927 902 742 732 490 399 329 157 134 85 63 59 56 BspEI T'CCGG_A Cuts at: 3909 7983 3909 Size: 4074 4246 BspHI T'CATG_A Cuts at: 5394 6209 7939 5394 Size: 815 1730 5775 Fragments arranged by size: 5775 1730 815 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2227 3583 4327 5675 844 Size: 1383 1356 744 1348 3489 Fragments arranged by size: 3489 1383 1356 1348 744 BsrBI CCG'CTC Cuts at: 1394 1823 6207 1394 Size: 429 4384 3507 Fragments arranged by size: 4384 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4119 6815 6989 65 Size: 1183 2871 2696 174 1396 Fragments arranged by size: 2871 2696 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3101 4188 4332 6964 7151 1700 Size: 110 1291 1087 144 2632 187 2869 Fragments arranged by size: 2869 2632 1291 1087 187 144 110 BsrGI T'GTAC_A Cuts at: 96 2155 3699 3768 5066 96 Size: 2059 1544 69 1298 3350 Fragments arranged by size: 3350 2059 1544 1298 69 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8320 BssSI C'ACGA_G Cuts at: 6380 7392 6380 Size: 1012 7308 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8320 BstBI TT'CG_AA Cuts at: 7124 7124 Size: 8320 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8320 BstXI CCAn_nnnn'nTGG Cuts at: 2044 3301 3505 2044 Size: 1257 204 6859 Fragments arranged by size: 6859 1257 204 BstYI r'GATC_y Cuts at: 1972 2218 3637 3975 4239 4944 5274 5650 6017 Size: 246 1419 338 264 705 330 376 367 Cuts at: 6017 6402 6419 7871 7957 7969 8315 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1419 705 385 376 367 346 338 330 264 246 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3182 3369 3891 4407 4948 513 Size: 552 2117 187 522 516 541 3885 Fragments arranged by size: 3885 2117 552 541 522 516 187 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4399 487 Size: 683 56 3173 4408 Fragments arranged by size: 4408 3173 683 56 BtsI GCAGTG_nn' Cuts at: 2552 3843 4497 5836 6630 6650 2552 Size: 1291 654 1339 794 20 4222 Fragments arranged by size: 4222 1339 1291 794 654 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8320 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8285 DraI TTT'AAA Cuts at: 5624 5624 Size: 8320 DraIII CAC_nnn'GTG Cuts at: 2254 2254 Size: 8320 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3515 7327 809 Size: 507 2199 3812 1802 Fragments arranged by size: 3812 2199 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 2803 4563 Size: 54 1425 50 275 18 216 757 1760 Cuts at: 4563 4674 4720 5767 6656 8285 8 Size: 111 46 1047 889 1629 43 Fragments arranged by size: 1760 1629 1425 1047 889 757 275 216 111 54 50 46 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5767 1537 Size: 275 3955 4090 Fragments arranged by size: 4090 3955 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2310 4925 5108 5451 6250 1353 Size: 957 2615 183 343 799 3423 Fragments arranged by size: 3423 2615 957 799 343 183 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6907 7281 7427 387 Size: 1064 138 5318 374 146 1280 Fragments arranged by size: 5318 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2715 2715 Size: 8320 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2243 2351 2720 3549 3662 4403 4428 Size: 393 611 108 369 829 113 741 25 Cuts at: 4428 4605 5044 5186 5195 6383 6980 7767 8035 Size: 177 439 142 9 1188 597 787 268 Cuts at: 8035 1239 Size: 1524 Fragments arranged by size: 1524 1188 829 787 741 611 597 439 393 369 268 177 142 113 108 25 9 EcoNI CCTnn'n_nnAGG Cuts at: 2193 5177 5450 2193 Size: 2984 273 5063 Fragments arranged by size: 5063 2984 273 EcoO109I rG'GnC_Cy Cuts at: 2781 4018 4258 5290 2781 Size: 1237 240 1032 5811 Fragments arranged by size: 5811 1237 1032 240 EcoRI G'AATT_C Cuts at: 4181 5408 5629 6007 4181 Size: 1227 221 378 6494 Fragments arranged by size: 6494 1227 378 221 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2299 4780 4981 2299 Size: 2481 201 5638 Fragments arranged by size: 5638 2481 201 FspI TGC'GCA Cuts at: 3719 3800 6826 3719 Size: 81 3026 5213 Fragments arranged by size: 5213 3026 81 HaeII r_GCGC'y Cuts at: 1678 3783 4714 7467 1678 Size: 2105 931 2753 2531 Fragments arranged by size: 2753 2531 2105 931 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2699 2731 3171 3203 3547 Size: 32 135 32 1127 32 440 32 344 Cuts at: 3547 3579 4494 4508 4526 4540 4578 4610 5259 Size: 32 915 14 18 14 38 32 649 Cuts at: 5259 5291 6961 6993 7035 7067 1373 Size: 32 1670 32 42 32 2626 Fragments arranged by size: 2626 1670 1127 915 649 440 344 135 42 38 32 32 32 32 32 32 32 32 32 18 14 14 HincII GTy'rAC Cuts at: 669 1657 1938 2594 2833 3414 5664 5910 669 Size: 988 281 656 239 581 2250 246 3079 Fragments arranged by size: 3079 2250 988 656 581 281 246 239 HindIII A'AGCT_T Cuts at: 748 3441 4250 748 Size: 2693 809 4818 Fragments arranged by size: 4818 2693 809 HpaI GTT'AAC Cuts at: 5910 5910 Size: 8320 KasI G'GCGC_C Cuts at: 3779 4710 3779 Size: 931 7389 KpnI G_GTAC'C Cuts at: 5645 5645 Size: 8320 MfeI C'AATT_G Cuts at: 5919 5919 Size: 8320 MluI A'CGCG_T Cuts at: 8196 8196 Size: 8320 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5490 5565 7434 7618 1305 Size: 4185 75 1869 184 2007 Fragments arranged by size: 4185 2007 1869 184 75 MscI TGG'CCA Cuts at: 10 64 1489 1832 4676 10 Size: 54 1425 343 2844 3654 Fragments arranged by size: 3654 2844 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2081 2489 3354 3431 4295 4751 4904 Size: 648 915 408 865 77 864 456 153 Cuts at: 4904 6278 6637 6796 518 Size: 1374 359 159 2042 Fragments arranged by size: 2042 1374 915 865 864 648 456 408 359 159 153 77 MspA1I CmG'CkG Cuts at: 1606 2822 5736 6413 7561 7806 1606 Size: 1216 2914 677 1148 245 2120 Fragments arranged by size: 2914 2120 1216 1148 677 245 NarI GG'CG_CC Cuts at: 3780 4711 3780 Size: 931 7389 NcoI C'CATG_G Cuts at: 513 1065 3182 3369 3891 4407 4948 513 Size: 552 2117 187 522 516 541 3885 Fragments arranged by size: 3885 2117 552 541 522 516 187 NdeI CA'TA_TG Cuts at: 387 5530 387 Size: 5143 3177 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8320 NotI GC'GGCC_GC Cuts at: 5767 5767 Size: 8320 NsiI A_TGCA'T Cuts at: 4777 4777 Size: 8320 NspI r_CATG'y Cuts at: 1132 1229 5678 7223 1132 Size: 97 4449 1545 2229 Fragments arranged by size: 4449 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8320 PciI A'CATG_T Cuts at: 7219 7219 Size: 8320 PflMI CCAn_nnn'nTGG Cuts at: 1108 2791 1108 Size: 1683 6637 PfoI T'CCnGG_A Cuts at: 2328 3504 3816 5445 2328 Size: 1176 312 1629 5203 Fragments arranged by size: 5203 1629 1176 312 PmeI GTTT'AAAC Cuts at: 5624 5624 Size: 8320 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2555 2587 6389 6421 7902 7934 2555 Size: 32 3802 32 1481 32 2941 Fragments arranged by size: 3802 2941 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2781 4018 4258 2781 Size: 1237 240 6843 Fragments arranged by size: 6843 1237 240 PshAI GACnn'nnGTC Cuts at: 1613 2786 1613 Size: 1173 7147 PsiI TTA'TAA Cuts at: 5890 5890 Size: 8320 PstI C_TGCA'G Cuts at: 830 2171 2218 2547 2671 3063 4539 5672 830 Size: 1341 47 329 124 392 1476 1133 3478 Fragments arranged by size: 3478 1476 1341 1133 392 329 124 47 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8320 PvuII CAG'CTG Cuts at: 2822 2822 Size: 8320 SacI G_AGCT'C Cuts at: 2717 2717 Size: 8320 SalI G'TCGA_C Cuts at: 1936 5662 1936 Size: 3726 4594 SanDI GG'GwC_CC Cuts at: 2781 2781 Size: 8320 SapI GCTCTTCn'nnn_ Cuts at: 2310 4925 2310 Size: 2615 5705 SbfI CC_TGCA'GG Cuts at: 5672 5672 Size: 8320 ScaI AGT'ACT Cuts at: 3218 6568 8280 3218 Size: 3350 1712 3258 Fragments arranged by size: 3350 3258 1712 SfcI C'TryA_G Cuts at: 826 1045 2167 2214 2543 2667 3059 4535 5001 Size: 219 1122 47 329 124 392 1476 466 Cuts at: 5001 5597 5668 6803 7484 7675 826 Size: 596 71 1135 681 191 1471 Fragments arranged by size: 1476 1471 1135 1122 681 596 466 392 329 219 191 124 71 47 SfoI GGC'GCC Cuts at: 3781 4712 3781 Size: 931 7389 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8320 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8320 SmaI CCC'GGG Cuts at: 1872 4115 5647 1872 Size: 2243 1532 4545 Fragments arranged by size: 4545 2243 1532 SmlI C'TyrA_G Cuts at: 820 1017 1943 6424 7325 7587 820 Size: 197 926 4481 901 262 1553 Fragments arranged by size: 4481 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6111 493 Size: 5618 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8320 SphI G_CATG'C Cuts at: 1132 5678 1132 Size: 4546 3774 SrfI GCCC'GGGC Cuts at: 4115 4115 Size: 8320 SspI AAT'ATT Cuts at: 5 52 6244 5 Size: 47 6192 2081 Fragments arranged by size: 6192 2081 47 StuI AGG'CCT Cuts at: 1517 3582 1517 Size: 2065 6255 StyI C'CwwG_G Cuts at: 513 1065 2686 3182 3369 3891 4407 4948 5214 Size: 552 1621 496 187 522 516 541 266 Cuts at: 5214 5759 513 Size: 545 3074 Fragments arranged by size: 3074 1621 552 545 541 522 516 496 266 187 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3623 4885 6347 6364 6517 6702 7147 Size: 1255 2043 1262 1462 17 153 185 445 Cuts at: 7147 325 Size: 1498 Fragments arranged by size: 2043 1498 1462 1262 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2155 3197 3216 Size: 274 80 33 51 1443 178 1042 19 Cuts at: 3216 3250 3699 3768 5066 6566 8278 96 Size: 34 449 69 1298 1500 1712 138 Fragments arranged by size: 1712 1500 1443 1298 1042 449 274 178 138 80 69 51 34 33 19 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2238 2270 8280 8312 2238 Size: 32 6010 32 2246 Fragments arranged by size: 6010 2246 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2024 2738 1316 Size: 708 714 6898 Fragments arranged by size: 6898 714 708 XbaI T'CTAG_A Cuts at: 5656 5656 Size: 8320 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2350 2350 Size: 8320 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8320 XmaI C'CCGG_G Cuts at: 1870 4113 5645 1870 Size: 2243 1532 4545 Fragments arranged by size: 4545 2243 1532 XmnI GAAnn'nnTTC Cuts at: 2304 2531 5355 6449 2304 Size: 227 2824 1094 4175 Fragments arranged by size: 4175 2824 1094 227 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6128 276 Size: 53 83 186 936 4594 2468 Fragments arranged by size: 4594 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI NaeI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SwaI