(Circular) (Six-base) MAPSORT of: pFN21AB6551.seq Check: 2578 from: 1 to: 5818 pFN21AB6551 5818 bp With 182 enzymes: SgfI * October 20, 2009 10:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3628 278 Size: 53 83 186 936 2092 2468 Fragments arranged by size: 2468 2092 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3161 1937 Size: 1224 4594 Acc65I G'GTAC_C Cuts at: 3139 3139 Size: 5818 AclI AA'CG_TT Cuts at: 1563 3945 4318 1563 Size: 2382 373 3063 Fragments arranged by size: 3063 2382 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2460 3881 5265 5533 1632 Size: 828 1421 1384 268 1917 Fragments arranged by size: 1917 1421 1384 828 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5621 AflIII A'CryG_T Cuts at: 1203 4717 5694 1203 Size: 3514 977 1327 Fragments arranged by size: 3514 1327 977 AgeI A'CCGG_T Cuts at: 4649 4649 Size: 5818 AhdI GACnn_n'nnGTC Cuts at: 2084 4547 2084 Size: 2463 3355 AleI CACnn'nnGTG Cuts at: 1166 2634 1166 Size: 1468 4350 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5818 AlwNI CAG_nnn'CTG Cuts at: 3561 5133 3561 Size: 1572 4246 ApaI G_GGCC'C Cuts at: 2032 3042 2032 Size: 1010 4808 ApaLI G'TGCA_C Cuts at: 3875 5031 3875 Size: 1156 4662 ApoI r'AATT_y Cuts at: 1493 2527 3127 3353 3505 5614 1493 Size: 1034 600 226 152 2109 1697 Fragments arranged by size: 2109 1697 1034 600 226 152 AseI AT'TA_AT Cuts at: 160 1032 4372 160 Size: 872 3340 1606 Fragments arranged by size: 3340 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5818 AvaI C'yCGr_G Cuts at: 1870 1943 2058 2820 2856 2889 2955 3143 1870 Size: 73 115 762 36 33 66 188 4545 Fragments arranged by size: 4545 762 188 115 73 66 36 33 BamHI G'GATC_C Cuts at: 3148 3515 3148 Size: 367 5451 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2904 3139 4594 618 Size: 325 217 627 1117 235 1455 1842 Fragments arranged by size: 1842 1455 1117 627 325 235 217 BanII G_rGCy'C Cuts at: 1391 2032 2313 3042 3065 3137 1391 Size: 641 281 729 23 72 4072 Fragments arranged by size: 4072 729 641 281 72 23 BbeI G_GCGC'C Cuts at: 2908 2908 Size: 5818 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4847 BbvCI CC'TCA_GC Cuts at: 2511 2511 Size: 5818 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4009 4043 1903 Size: 34 2072 34 3678 Fragments arranged by size: 3678 2072 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3710 4920 3710 Size: 1210 4608 BclI T'GATC_A Cuts at: 1548 2680 1548 Size: 1132 4686 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4429 136 Size: 107 122 71 631 605 2757 1525 Fragments arranged by size: 2757 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2846 5813 2846 Size: 2967 2851 BlpI GC'TnA_GC Cuts at: 3235 3235 Size: 5818 Bme1580I G_kGCm'C Cuts at: 1790 2032 2054 3042 3879 5035 1790 Size: 242 22 988 837 1156 2573 Fragments arranged by size: 2573 1156 988 837 242 22 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5818 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3549 4507 442 Size: 416 53 83 399 24 2132 958 1753 Fragments arranged by size: 2132 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5818 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2968 3070 4478 1239 Size: 1729 102 1408 2579 Fragments arranged by size: 2579 1729 1408 102 Bpu10I CC'TnA_GC Cuts at: 2511 2511 Size: 5818 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2755 3077 3943 4808 5106 2755 Size: 322 866 865 298 3467 Fragments arranged by size: 3467 866 865 322 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2232 2603 4481 882 Size: 617 733 371 1878 2219 Fragments arranged by size: 2219 1878 733 617 371 BsaAI yAC'GTr Cuts at: 493 1204 3609 493 Size: 711 2405 2702 Fragments arranged by size: 2702 2405 711 BsaBI GATnn'nnATC Cuts at: 2898 5812 2898 Size: 2914 2904 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2830 2905 3625 4007 Size: 53 83 186 936 1297 75 720 382 Cuts at: 4007 4642 275 Size: 635 1451 Fragments arranged by size: 1451 1297 936 720 635 382 186 83 75 53 BsaWI w'CCGG_w Cuts at: 4251 4649 4923 5070 5481 4251 Size: 398 274 147 411 4588 Fragments arranged by size: 4588 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2966 2996 1178 Size: 30 165 30 1563 30 4000 Fragments arranged by size: 4000 1563 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2752 2755 2762 2777 3020 1186 Size: 1566 3 7 15 243 3984 Fragments arranged by size: 3984 1566 243 15 7 3 BseYI C'CCAG_C Cuts at: 2153 5021 5517 2153 Size: 2868 496 2454 Fragments arranged by size: 2868 2454 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5245 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3268 4029 5057 1540 Size: 275 186 1267 761 1028 2301 Fragments arranged by size: 2301 1267 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2313 3065 3879 3964 5035 2313 Size: 752 814 85 1071 3096 Fragments arranged by size: 3096 1071 814 752 85 BsmI GAATG_Cn' Cuts at: 3329 3422 3329 Size: 93 5725 BsmBI CGTCTCn'nnnn_ Cuts at: 2061 2061 Size: 5818 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2032 2054 2313 3042 3065 3137 3879 Size: 399 242 22 259 729 23 72 742 Cuts at: 3879 3964 5035 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 729 399 259 242 85 72 23 22 BspEI T'CCGG_A Cuts at: 5481 5481 Size: 5818 BspHI T'CATG_A Cuts at: 3707 5437 3707 Size: 1730 4088 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3173 844 Size: 2329 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2992 3021 3705 1394 Size: 429 1169 29 684 3507 Fragments arranged by size: 3507 1169 684 429 29 BsrDI GCAATG_nn' Cuts at: 65 1248 2268 4313 4487 65 Size: 1183 1020 2045 174 1396 Fragments arranged by size: 2045 1396 1183 1020 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3107 4462 4649 1700 Size: 110 1297 1355 187 2869 Fragments arranged by size: 2869 1355 1297 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5818 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5818 BssSI C'ACGA_G Cuts at: 3878 4890 3878 Size: 1012 4806 BstAPI GCAn_nnn'nTGC Cuts at: 794 2563 794 Size: 1769 4049 BstBI TT'CG_AA Cuts at: 4622 4622 Size: 5818 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5818 BstXI CCAn_nnnn'nTGG Cuts at: 2636 2636 Size: 5818 BstYI r'GATC_y Cuts at: 1972 2664 2846 3148 3515 3900 3917 5369 5455 Size: 692 182 302 367 385 17 1452 86 Cuts at: 5455 5467 5813 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 692 385 367 346 302 182 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5266 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5079 Fragments arranged by size: 5079 683 56 BtsI GCAGTG_nn' Cuts at: 3334 4128 4148 3334 Size: 794 20 5004 Fragments arranged by size: 5004 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5818 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5783 DraI TTT'AAA Cuts at: 3122 3122 Size: 5818 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4825 809 Size: 507 3509 1802 Fragments arranged by size: 3509 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3265 4154 5783 Size: 54 1425 50 275 18 1435 889 1629 Cuts at: 5783 8 Size: 43 Fragments arranged by size: 1629 1435 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3265 1537 Size: 275 1453 4090 Fragments arranged by size: 4090 1453 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2711 3090 3748 1353 Size: 1358 379 658 3423 Fragments arranged by size: 3423 1358 658 379 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 1999 3040 4405 4779 4925 387 Size: 1064 138 410 1041 1365 374 146 1280 Fragments arranged by size: 1365 1280 1064 1041 410 374 146 138 EcoICRI GAG'CTC Cuts at: 2311 3063 2311 Size: 752 5066 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2460 2968 3070 3881 4478 5265 5533 Size: 393 828 508 102 811 597 787 268 Cuts at: 5533 1239 Size: 1524 Fragments arranged by size: 1524 828 811 787 597 508 393 268 102 EcoO109I rG'GnC_Cy Cuts at: 2029 3038 3039 2029 Size: 1009 1 4808 Fragments arranged by size: 4808 1009 1 EcoRI G'AATT_C Cuts at: 3127 3505 3127 Size: 378 5440 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2665 2665 Size: 5818 FspI TGC'GCA Cuts at: 4324 4324 Size: 5818 HaeII r_GCGC'y Cuts at: 1678 2908 4965 1678 Size: 1230 2057 2531 Fragments arranged by size: 2531 2057 1230 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2070 2102 2981 3013 4459 Size: 32 135 32 498 32 879 32 1446 Cuts at: 4459 4491 4533 4565 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1446 879 498 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3162 3408 669 Size: 988 281 1224 246 3079 Fragments arranged by size: 3079 1224 988 281 246 HindIII A'AGCT_T Cuts at: 748 2675 748 Size: 1927 3891 HpaI GTT'AAC Cuts at: 3408 3408 Size: 5818 KasI G'GCGC_C Cuts at: 2904 2904 Size: 5818 KpnI G_GTAC'C Cuts at: 3143 3143 Size: 5818 MfeI C'AATT_G Cuts at: 3417 3417 Size: 5818 MluI A'CGCG_T Cuts at: 5694 5694 Size: 5818 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2813 3090 4932 5116 1305 Size: 1508 277 1842 184 2007 Fragments arranged by size: 2007 1842 1508 277 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3996 Fragments arranged by size: 3996 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2607 2634 3776 4135 4294 518 Size: 648 1441 27 1142 359 159 2042 Fragments arranged by size: 2042 1441 1142 648 359 159 27 MspA1I CmG'CkG Cuts at: 1606 3234 3911 5059 5304 1606 Size: 1628 677 1148 245 2120 Fragments arranged by size: 2120 1628 1148 677 245 NaeI GCC'GGC Cuts at: 3109 3109 Size: 5818 NarI GG'CG_CC Cuts at: 2905 2905 Size: 5818 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5266 NdeI CA'TA_TG Cuts at: 387 387 Size: 5818 NgoMIV G'CCGG_C Cuts at: 3107 3107 Size: 5818 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5818 NotI GC'GGCC_GC Cuts at: 3265 3265 Size: 5818 NspI r_CATG'y Cuts at: 1132 1229 3176 4721 1132 Size: 97 1947 1545 2229 Fragments arranged by size: 2229 1947 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5818 PciI A'CATG_T Cuts at: 4717 4717 Size: 5818 PflMI CCAn_nnn'nTGG Cuts at: 1108 2047 2197 1108 Size: 939 150 4729 Fragments arranged by size: 4729 939 150 PfoI T'CCnGG_A Cuts at: 2921 2921 Size: 5818 PmeI GTTT'AAAC Cuts at: 3122 3122 Size: 5818 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3887 3919 5400 5432 3887 Size: 32 1481 32 4273 Fragments arranged by size: 4273 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5818 PsiI TTA'TAA Cuts at: 3388 3388 Size: 5818 PspOMI G'GGCC_C Cuts at: 2028 3038 2028 Size: 1010 4808 PstI C_TGCA'G Cuts at: 830 3170 830 Size: 2340 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5818 SacI G_AGCT'C Cuts at: 2313 3065 2313 Size: 752 5066 SalI G'TCGA_C Cuts at: 1936 3160 1936 Size: 1224 4594 SbfI CC_TGCA'GG Cuts at: 3170 3170 Size: 5818 ScaI AGT'ACT Cuts at: 4066 5778 4066 Size: 1712 4106 SfcI C'TryA_G Cuts at: 826 1045 3166 4301 4982 5173 826 Size: 219 2121 1135 681 191 1471 Fragments arranged by size: 2121 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2906 2906 Size: 5818 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5818 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5818 SmaI CCC'GGG Cuts at: 1872 2822 2957 3145 1872 Size: 950 135 188 4545 Fragments arranged by size: 4545 950 188 135 SmlI C'TyrA_G Cuts at: 820 1017 1943 2734 3056 3922 4823 5085 820 Size: 197 926 791 322 866 901 262 1553 Fragments arranged by size: 1553 926 901 866 791 322 262 197 SnaBI TAC'GTA Cuts at: 493 3609 493 Size: 3116 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5818 SphI G_CATG'C Cuts at: 1132 3176 1132 Size: 2044 3774 SspI AAT'ATT Cuts at: 5 52 3742 5 Size: 47 3690 2081 Fragments arranged by size: 3690 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5818 StyI C'CwwG_G Cuts at: 513 1065 2593 3257 513 Size: 552 1528 664 3074 Fragments arranged by size: 3074 1528 664 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2976 3845 3862 4015 4200 4645 325 Size: 1255 1396 869 17 153 185 445 1498 Fragments arranged by size: 1498 1396 1255 869 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2380 4064 5776 Size: 274 80 33 51 1443 403 1684 1712 Cuts at: 5776 96 Size: 138 Fragments arranged by size: 1712 1684 1443 403 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2261 2293 5778 5810 2261 Size: 32 3485 32 2269 Fragments arranged by size: 3485 2269 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2105 2943 1316 Size: 789 838 4191 Fragments arranged by size: 4191 838 789 XbaI T'CTAG_A Cuts at: 3154 3154 Size: 5818 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2323 3073 2323 Size: 750 5068 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5818 XmaI C'CCGG_G Cuts at: 1870 2820 2955 3143 1870 Size: 950 135 188 4545 Fragments arranged by size: 4545 950 188 135 XmnI GAAnn'nnTTC Cuts at: 2487 3947 2487 Size: 1460 4358 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3626 276 Size: 53 83 186 936 2092 2468 Fragments arranged by size: 2468 2092 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI