(Circular) (Six-base) MAPSORT of: pFN21AB6541.seq Check: 1223 from: 1 to: 5788 pFN21AB6541 5788 bp With 182 enzymes: SgfI * October 20, 2009 10:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3598 278 Size: 53 83 186 936 2062 2468 Fragments arranged by size: 2468 2062 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2659 2810 3131 1937 Size: 722 151 321 4594 Fragments arranged by size: 4594 722 321 151 Acc65I G'GTAC_C Cuts at: 2394 3109 2394 Size: 715 5073 AclI AA'CG_TT Cuts at: 1563 3915 4288 1563 Size: 2352 373 3063 Fragments arranged by size: 3063 2352 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3851 5235 5503 1632 Size: 2219 1384 268 1917 Fragments arranged by size: 2219 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5591 AflIII A'CryG_T Cuts at: 1203 2246 2805 4687 5664 1203 Size: 1043 559 1882 977 1327 Fragments arranged by size: 1882 1327 1043 977 559 AgeI A'CCGG_T Cuts at: 4619 4619 Size: 5788 AhdI GACnn_n'nnGTC Cuts at: 4517 4517 Size: 5788 AleI CACnn'nnGTG Cuts at: 1166 2259 1166 Size: 1093 4695 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5788 AlwNI CAG_nnn'CTG Cuts at: 3531 5103 3531 Size: 1572 4216 ApaI G_GGCC'C Cuts at: 2867 2867 Size: 5788 ApaLI G'TGCA_C Cuts at: 3845 5001 3845 Size: 1156 4632 ApoI r'AATT_y Cuts at: 1493 2962 3031 3097 3323 3475 5584 1493 Size: 1469 69 66 226 152 2109 1697 Fragments arranged by size: 2109 1697 1469 226 152 69 66 AseI AT'TA_AT Cuts at: 160 1032 4342 160 Size: 872 3310 1606 Fragments arranged by size: 3310 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5788 AvaI C'yCGr_G Cuts at: 1870 1943 2058 3113 1870 Size: 73 115 1055 4545 Fragments arranged by size: 4545 1055 115 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2378 2411 2378 Size: 33 5755 BamHI G'GATC_C Cuts at: 2485 3118 3485 2485 Size: 633 367 4788 Fragments arranged by size: 4788 633 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2394 2557 3109 4564 618 Size: 325 217 627 607 163 552 1455 1842 Fragments arranged by size: 1842 1455 627 607 552 325 217 163 BanII G_rGCy'C Cuts at: 1391 2110 2867 3107 1391 Size: 719 757 240 4072 Fragments arranged by size: 4072 757 719 240 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4817 BbvCI CC'TCA_GC Cuts at: 2704 2704 Size: 5788 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3979 4013 1903 Size: 34 2042 34 3678 Fragments arranged by size: 3678 2042 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2418 3680 4890 2418 Size: 1262 1210 3316 Fragments arranged by size: 3316 1262 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5788 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4399 136 Size: 107 122 71 631 605 2727 1525 Fragments arranged by size: 2727 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5783 5783 Size: 5788 BlpI GC'TnA_GC Cuts at: 3205 3205 Size: 5788 Bme1580I G_kGCm'C Cuts at: 1790 2562 2867 3002 3849 5005 1790 Size: 772 305 135 847 1156 2573 Fragments arranged by size: 2573 1156 847 772 305 135 BmgBI CAC'GTC Cuts at: 1318 2240 1318 Size: 922 4866 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2298 2469 3519 Size: 416 53 83 399 24 881 171 1050 Cuts at: 3519 4477 442 Size: 958 1753 Fragments arranged by size: 1753 1050 958 881 416 399 171 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5788 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2556 3079 2556 Size: 523 5265 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2214 2421 2718 4448 1239 Size: 975 207 297 1730 2579 Fragments arranged by size: 2579 1730 975 297 207 Bpu10I CC'TnA_GC Cuts at: 2704 2935 2704 Size: 231 5557 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2242 3913 4778 5076 2242 Size: 1671 865 298 2954 Fragments arranged by size: 2954 1671 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2587 4451 882 Size: 617 1088 1864 2219 Fragments arranged by size: 2219 1864 1088 617 BsaAI yAC'GTr Cuts at: 493 1204 2080 3579 493 Size: 711 876 1499 2702 Fragments arranged by size: 2702 1499 876 711 BsaBI GATnn'nnATC Cuts at: 5782 5782 Size: 5788 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3595 3977 4612 275 Size: 53 83 186 936 2062 382 635 1451 Fragments arranged by size: 2062 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4221 4619 4893 5040 5451 4221 Size: 398 274 147 411 4558 Fragments arranged by size: 4558 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5563 Fragments arranged by size: 5563 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2532 2692 1186 Size: 1346 160 4282 Fragments arranged by size: 4282 1346 160 BseYI C'CCAG_C Cuts at: 4991 5487 4991 Size: 496 5292 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2244 1163 Size: 573 508 4707 Fragments arranged by size: 4707 573 508 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3238 3999 5027 1540 Size: 275 186 1237 761 1028 2301 Fragments arranged by size: 2301 1237 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2110 3849 3934 5005 2110 Size: 1739 85 1071 2893 Fragments arranged by size: 2893 1739 1071 85 BsmI GAATG_Cn' Cuts at: 2611 2631 2827 3299 3392 2611 Size: 20 196 472 93 5007 Fragments arranged by size: 5007 472 196 93 20 BsmBI CGTCTCn'nnnn_ Cuts at: 2230 2230 Size: 5788 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2110 2562 2867 3002 3107 3849 3934 Size: 399 320 452 305 135 105 742 85 Cuts at: 3934 5005 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 452 399 320 305 135 105 85 BspEI T'CCGG_A Cuts at: 5451 5451 Size: 5788 BspHI T'CATG_A Cuts at: 3677 5407 3677 Size: 1730 4058 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3143 844 Size: 2299 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3675 1394 Size: 429 1852 3507 Fragments arranged by size: 3507 1852 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2595 4283 4457 65 Size: 1183 1347 1688 174 1396 Fragments arranged by size: 1688 1396 1347 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4432 4619 1700 Size: 110 2622 187 2869 Fragments arranged by size: 2869 2622 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5788 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5788 BssSI C'ACGA_G Cuts at: 3848 4860 3848 Size: 1012 4776 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5788 BstBI TT'CG_AA Cuts at: 4592 4592 Size: 5788 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5788 BstXI CCAn_nnnn'nTGG Cuts at: 2482 2482 Size: 5788 BstYI r'GATC_y Cuts at: 1972 2212 2485 3118 3485 3870 3887 5339 5425 Size: 240 273 633 367 385 17 1452 86 Cuts at: 5425 5437 5783 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 633 385 367 346 273 240 86 17 12 BstZ17I GTA'TAC Cuts at: 2811 2811 Size: 5788 Bsu36I CC'TnA_GG Cuts at: 2563 2563 Size: 5788 BtgI C'CryG_G Cuts at: 513 1065 2883 513 Size: 552 1818 3418 Fragments arranged by size: 3418 1818 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5049 Fragments arranged by size: 5049 683 56 BtsI GCAGTG_nn' Cuts at: 3304 4098 4118 3304 Size: 794 20 4974 Fragments arranged by size: 4974 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5788 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3066 3101 529 Size: 35 2502 35 3216 Fragments arranged by size: 3216 2502 35 35 DraI TTT'AAA Cuts at: 3092 3092 Size: 5788 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4795 809 Size: 507 3479 1802 Fragments arranged by size: 3479 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2666 3235 4124 Size: 54 1425 50 275 18 836 569 889 Cuts at: 4124 5753 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 836 569 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3235 1537 Size: 275 1423 4090 Fragments arranged by size: 4090 1423 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3718 1353 Size: 2365 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4375 4749 4895 387 Size: 1064 138 2786 374 146 1280 Fragments arranged by size: 2786 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2108 2108 Size: 5788 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2214 2421 2718 3851 4448 5235 5503 Size: 393 582 207 297 1133 597 787 268 Cuts at: 5503 1239 Size: 1524 Fragments arranged by size: 1524 1133 787 597 582 393 297 268 207 EcoNI CCTnn'n_nnAGG Cuts at: 2547 2736 2547 Size: 189 5599 EcoO109I rG'GnC_Cy Cuts at: 2351 2412 2729 2864 2351 Size: 61 317 135 5275 Fragments arranged by size: 5275 317 135 61 EcoRI G'AATT_C Cuts at: 2962 3097 3475 2962 Size: 135 378 5275 Fragments arranged by size: 5275 378 135 FspI TGC'GCA Cuts at: 4294 4294 Size: 5788 HaeII r_GCGC'y Cuts at: 1678 2037 4935 1678 Size: 359 2898 2531 Fragments arranged by size: 2898 2531 359 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2416 2448 4429 4461 4503 Size: 32 135 32 844 32 1981 32 42 Cuts at: 4503 4535 1373 Size: 32 2626 Fragments arranged by size: 2626 1981 844 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3132 3378 669 Size: 988 281 1194 246 3079 Fragments arranged by size: 3079 1194 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5788 HpaI GTT'AAC Cuts at: 3378 3378 Size: 5788 KpnI G_GTAC'C Cuts at: 2398 3113 2398 Size: 715 5073 MfeI C'AATT_G Cuts at: 3387 3387 Size: 5788 MluI A'CGCG_T Cuts at: 5664 5664 Size: 5788 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2070 2513 4902 5086 1305 Size: 765 443 2389 184 2007 Fragments arranged by size: 2389 2007 765 443 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2668 10 Size: 54 1425 343 836 3130 Fragments arranged by size: 3130 1425 836 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2259 2919 3077 3746 4105 4264 518 Size: 648 1093 660 158 669 359 159 2042 Fragments arranged by size: 2042 1093 669 660 648 359 159 158 MspA1I CmG'CkG Cuts at: 1606 3204 3881 5029 5274 1606 Size: 1598 677 1148 245 2120 Fragments arranged by size: 2120 1598 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2883 513 Size: 552 1818 3418 Fragments arranged by size: 3418 1818 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5788 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5788 NotI GC'GGCC_GC Cuts at: 3235 3235 Size: 5788 NspI r_CATG'y Cuts at: 1132 1229 2250 2344 2809 3146 4691 1132 Size: 97 1021 94 465 337 1545 2229 Fragments arranged by size: 2229 1545 1021 465 337 97 94 PasI CC'CwG_GG Cuts at: 1348 2867 1348 Size: 1519 4269 PciI A'CATG_T Cuts at: 2246 2805 4687 2246 Size: 559 1882 3347 Fragments arranged by size: 3347 1882 559 PflMI CCAn_nnn'nTGG Cuts at: 1108 2336 2654 2741 1108 Size: 1228 318 87 4155 Fragments arranged by size: 4155 1228 318 87 PfoI T'CCnGG_A Cuts at: 2734 2833 2734 Size: 99 5689 PmeI GTTT'AAAC Cuts at: 3092 3092 Size: 5788 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3857 3889 5370 5402 3857 Size: 32 1481 32 4243 Fragments arranged by size: 4243 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2351 2729 2351 Size: 378 5410 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5788 PsiI TTA'TAA Cuts at: 3358 3358 Size: 5788 PspOMI G'GGCC_C Cuts at: 2863 2863 Size: 5788 PstI C_TGCA'G Cuts at: 830 2296 3140 830 Size: 1466 844 3478 Fragments arranged by size: 3478 1466 844 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5788 SacI G_AGCT'C Cuts at: 2110 2110 Size: 5788 SalI G'TCGA_C Cuts at: 1936 3130 1936 Size: 1194 4594 SbfI CC_TGCA'GG Cuts at: 3140 3140 Size: 5788 ScaI AGT'ACT Cuts at: 4036 5748 4036 Size: 1712 4076 SfcI C'TryA_G Cuts at: 826 1045 2292 3136 4271 4952 5143 826 Size: 219 1247 844 1135 681 191 1471 Fragments arranged by size: 1471 1247 1135 844 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5788 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5788 SmaI CCC'GGG Cuts at: 1872 2060 3115 1872 Size: 188 1055 4545 Fragments arranged by size: 4545 1055 188 SmlI C'TyrA_G Cuts at: 820 1017 1943 2257 3892 4793 5055 820 Size: 197 926 314 1635 901 262 1553 Fragments arranged by size: 1635 1553 926 901 314 262 197 SnaBI TAC'GTA Cuts at: 493 2080 3579 493 Size: 1587 1499 2702 Fragments arranged by size: 2702 1587 1499 SpeI A'CTAG_T Cuts at: 152 152 Size: 5788 SphI G_CATG'C Cuts at: 1132 2344 3146 1132 Size: 1212 802 3774 Fragments arranged by size: 3774 1212 802 SspI AAT'ATT Cuts at: 5 52 3712 5 Size: 47 3660 2081 Fragments arranged by size: 3660 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5788 StyI C'CwwG_G Cuts at: 513 1065 2408 2663 2883 3005 3227 513 Size: 552 1343 255 220 122 222 3074 Fragments arranged by size: 3074 1343 552 255 222 220 122 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2507 2776 2876 3815 3832 3985 4170 Size: 1255 927 269 100 939 17 153 185 Cuts at: 4170 4615 325 Size: 445 1498 Fragments arranged by size: 1498 1255 939 927 445 269 185 153 100 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4034 5746 96 Size: 274 80 33 51 1443 2057 1712 138 Fragments arranged by size: 2057 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5748 5780 5748 Size: 32 5756 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5788 XbaI T'CTAG_A Cuts at: 3124 3124 Size: 5788 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5788 XmaI C'CCGG_G Cuts at: 1870 2058 3113 1870 Size: 188 1055 4545 Fragments arranged by size: 4545 1055 188 XmnI GAAnn'nnTTC Cuts at: 3917 3917 Size: 5788 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3596 276 Size: 53 83 186 936 2062 2468 Fragments arranged by size: 2468 2062 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BfrBI BsiWI DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI