(Circular) (Six-base) MAPSORT of: pFN21AB6514.seq Check: 7462 from: 1 to: 5818 pFN21AB6514 5818 bp With 182 enzymes: SgfI * October 20, 2009 10:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3628 278 Size: 53 83 186 936 2092 2468 Fragments arranged by size: 2468 2092 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2880 3161 1937 Size: 943 281 4594 Fragments arranged by size: 4594 943 281 Acc65I G'GTAC_C Cuts at: 3057 3139 3057 Size: 82 5736 AclI AA'CG_TT Cuts at: 1563 3945 4318 1563 Size: 2382 373 3063 Fragments arranged by size: 3063 2382 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3881 5265 5533 1632 Size: 2249 1384 268 1917 Fragments arranged by size: 2249 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5621 AflIII A'CryG_T Cuts at: 1203 4717 5694 1203 Size: 3514 977 1327 Fragments arranged by size: 3514 1327 977 AgeI A'CCGG_T Cuts at: 4649 4649 Size: 5818 AhdI GACnn_n'nnGTC Cuts at: 4547 4547 Size: 5818 AleI CACnn'nnGTG Cuts at: 1166 2528 1166 Size: 1362 4456 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5818 AlwNI CAG_nnn'CTG Cuts at: 2109 2680 2861 3561 5133 2109 Size: 571 181 700 1572 2794 Fragments arranged by size: 2794 1572 700 571 181 ApaLI G'TGCA_C Cuts at: 3875 5031 3875 Size: 1156 4662 ApoI r'AATT_y Cuts at: 1493 2201 3127 3353 3505 5614 1493 Size: 708 926 226 152 2109 1697 Fragments arranged by size: 2109 1697 926 708 226 152 AseI AT'TA_AT Cuts at: 160 1032 4372 160 Size: 872 3340 1606 Fragments arranged by size: 3340 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5818 AvaI C'yCGr_G Cuts at: 1870 1943 3143 1870 Size: 73 1200 4545 Fragments arranged by size: 4545 1200 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2473 2506 2473 Size: 33 5785 BamHI G'GATC_C Cuts at: 3148 3515 3148 Size: 367 5451 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3057 3139 4594 618 Size: 325 217 627 1270 82 1455 1842 Fragments arranged by size: 1842 1455 1270 627 325 217 82 BanII G_rGCy'C Cuts at: 1391 2396 3137 1391 Size: 1005 741 4072 Fragments arranged by size: 4072 1005 741 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4847 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2977 3011 4009 4043 1903 Size: 34 1040 34 998 34 3678 Fragments arranged by size: 3678 1040 998 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3710 4920 3710 Size: 1210 4608 BclI T'GATC_A Cuts at: 1548 2515 1548 Size: 967 4851 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4429 136 Size: 107 122 71 631 605 2757 1525 Fragments arranged by size: 2757 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5813 5813 Size: 5818 BlpI GC'TnA_GC Cuts at: 3235 3235 Size: 5818 Bme1580I G_kGCm'C Cuts at: 1790 3879 5035 1790 Size: 2089 1156 2573 Fragments arranged by size: 2573 2089 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5818 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2871 3549 4507 Size: 416 53 83 399 24 1454 678 958 Cuts at: 4507 442 Size: 1753 Fragments arranged by size: 1753 1454 958 678 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5818 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2077 2339 2483 4478 1239 Size: 838 262 144 1995 2579 Fragments arranged by size: 2579 1995 838 262 144 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3943 4808 5106 3943 Size: 865 298 4655 Fragments arranged by size: 4655 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2380 4481 882 Size: 617 881 2101 2219 Fragments arranged by size: 2219 2101 881 617 BsaAI yAC'GTr Cuts at: 493 1204 3609 493 Size: 711 2405 2702 Fragments arranged by size: 2702 2405 711 BsaBI GATnn'nnATC Cuts at: 5812 5812 Size: 5818 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3625 4007 4642 275 Size: 53 83 186 936 2092 382 635 1451 Fragments arranged by size: 2092 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4251 4649 4923 5070 5481 4251 Size: 398 274 147 411 4588 Fragments arranged by size: 4588 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2212 2242 2337 2367 3060 Size: 30 165 30 809 30 95 30 693 Cuts at: 3060 3090 1178 Size: 30 3906 Fragments arranged by size: 3906 809 693 165 95 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5818 BseYI C'CCAG_C Cuts at: 2612 2657 2688 5021 5517 2612 Size: 45 31 2333 496 2913 Fragments arranged by size: 2913 2333 496 45 31 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5245 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2488 3268 4029 5057 1540 Size: 275 186 487 780 761 1028 2301 Fragments arranged by size: 2301 1028 780 761 487 275 186 BsiHKAI G_wGCw'C Cuts at: 2396 3879 3964 5035 2396 Size: 1483 85 1071 3179 Fragments arranged by size: 3179 1483 1071 85 BsmI GAATG_Cn' Cuts at: 3329 3422 3329 Size: 93 5725 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2396 3137 3879 3964 5035 1391 Size: 399 606 741 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 741 606 399 85 BspEI T'CCGG_A Cuts at: 5481 5481 Size: 5818 BspHI T'CATG_A Cuts at: 3707 5437 3707 Size: 1730 4088 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3173 844 Size: 2329 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3705 1394 Size: 429 1882 3507 Fragments arranged by size: 3507 1882 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2089 4313 4487 65 Size: 1183 841 2224 174 1396 Fragments arranged by size: 2224 1396 1183 841 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2709 4462 4649 1700 Size: 110 899 1753 187 2869 Fragments arranged by size: 2869 1753 899 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5818 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5818 BssSI C'ACGA_G Cuts at: 3878 4890 3878 Size: 1012 4806 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5818 BstBI TT'CG_AA Cuts at: 4622 4622 Size: 5818 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5818 BstXI CCAn_nnnn'nTGG Cuts at: 2542 2542 Size: 5818 BstYI r'GATC_y Cuts at: 1972 3148 3515 3900 3917 5369 5455 5467 5813 Size: 1176 367 385 17 1452 86 12 346 Cuts at: 5813 1972 Size: 1977 Fragments arranged by size: 1977 1452 1176 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2049 2049 Size: 5818 BtgI C'CryG_G Cuts at: 513 1065 2004 2040 3033 513 Size: 552 939 36 993 3298 Fragments arranged by size: 3298 993 939 552 36 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3025 487 Size: 683 56 1799 3280 Fragments arranged by size: 3280 1799 683 56 BtsI GCAGTG_nn' Cuts at: 2949 3334 4128 4148 2949 Size: 385 794 20 4619 Fragments arranged by size: 4619 794 385 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5818 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2811 2846 529 Size: 35 2247 35 3501 Fragments arranged by size: 3501 2247 35 35 DraI TTT'AAA Cuts at: 2340 3094 3122 2340 Size: 754 28 5036 Fragments arranged by size: 5036 754 28 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4825 809 Size: 507 3509 1802 Fragments arranged by size: 3509 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2485 2602 3030 Size: 54 1425 50 275 18 655 117 428 Cuts at: 3030 3036 3265 4154 5783 8 Size: 6 229 889 1629 43 Fragments arranged by size: 1629 1425 889 655 428 275 229 117 54 50 43 18 6 EagI C'GGCC_G Cuts at: 1537 1812 2485 3265 1537 Size: 275 673 780 4090 Fragments arranged by size: 4090 780 673 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2219 3748 1353 Size: 866 1529 3423 Fragments arranged by size: 3423 1529 866 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4405 4779 4925 387 Size: 1064 138 2816 374 146 1280 Fragments arranged by size: 2816 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2394 2394 Size: 5818 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2077 2339 2483 3881 4478 5265 5533 Size: 393 445 262 144 1398 597 787 268 Cuts at: 5533 1239 Size: 1524 Fragments arranged by size: 1524 1398 787 597 445 393 268 262 144 EcoNI CCTnn'n_nnAGG Cuts at: 2404 2404 Size: 5818 EcoO109I rG'GnC_Cy Cuts at: 2677 2677 Size: 5818 EcoRI G'AATT_C Cuts at: 2201 3127 3505 2201 Size: 926 378 4514 Fragments arranged by size: 4514 926 378 FspI TGC'GCA Cuts at: 4324 4324 Size: 5818 HaeII r_GCGC'y Cuts at: 1678 2716 4965 1678 Size: 1038 2249 2531 Fragments arranged by size: 2531 2249 1038 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2337 2369 4459 4491 4533 Size: 32 135 32 765 32 2090 32 42 Cuts at: 4533 4565 1373 Size: 32 2626 Fragments arranged by size: 2626 2090 765 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2170 2296 3162 3408 669 Size: 988 281 232 126 866 246 3079 Fragments arranged by size: 3079 988 866 281 246 232 126 HindIII A'AGCT_T Cuts at: 748 748 Size: 5818 HpaI GTT'AAC Cuts at: 2296 3408 2296 Size: 1112 4706 KpnI G_GTAC'C Cuts at: 3061 3143 3061 Size: 82 5736 MfeI C'AATT_G Cuts at: 3417 3417 Size: 5818 MluI A'CGCG_T Cuts at: 5694 5694 Size: 5818 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2090 4932 5116 1305 Size: 785 2842 184 2007 Fragments arranged by size: 2842 2007 785 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2604 3038 10 Size: 54 1425 343 772 434 2790 Fragments arranged by size: 2790 1425 772 434 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2528 3776 4135 4294 518 Size: 648 1362 1248 359 159 2042 Fragments arranged by size: 2042 1362 1248 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2616 3234 3911 5059 5304 1606 Size: 1010 618 677 1148 245 2120 Fragments arranged by size: 2120 1148 1010 677 618 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2040 3033 513 Size: 552 939 36 993 3298 Fragments arranged by size: 3298 993 939 552 36 NdeI CA'TA_TG Cuts at: 387 2380 387 Size: 1993 3825 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5818 NotI GC'GGCC_GC Cuts at: 3265 3265 Size: 5818 NspI r_CATG'y Cuts at: 1132 1229 3176 4721 1132 Size: 97 1947 1545 2229 Fragments arranged by size: 2229 1947 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5818 PciI A'CATG_T Cuts at: 4717 4717 Size: 5818 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 1108 Size: 902 4916 PmeI GTTT'AAAC Cuts at: 3122 3122 Size: 5818 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3887 3919 5400 5432 3887 Size: 32 1481 32 4273 Fragments arranged by size: 4273 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5818 PsiI TTA'TAA Cuts at: 3388 3388 Size: 5818 PstI C_TGCA'G Cuts at: 830 3170 830 Size: 2340 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5818 SacI G_AGCT'C Cuts at: 2396 2396 Size: 5818 SalI G'TCGA_C Cuts at: 1936 3160 1936 Size: 1224 4594 SbfI CC_TGCA'GG Cuts at: 3170 3170 Size: 5818 ScaI AGT'ACT Cuts at: 4066 5778 4066 Size: 1712 4106 SfcI C'TryA_G Cuts at: 826 1045 3166 4301 4982 5173 826 Size: 219 2121 1135 681 191 1471 Fragments arranged by size: 2121 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5818 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5818 SmaI CCC'GGG Cuts at: 1872 3145 1872 Size: 1273 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3922 4823 5085 820 Size: 197 926 1979 901 262 1553 Fragments arranged by size: 1979 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3609 493 Size: 3116 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5818 SphI G_CATG'C Cuts at: 1132 3176 1132 Size: 2044 3774 SspI AAT'ATT Cuts at: 5 52 3742 5 Size: 47 3690 2081 Fragments arranged by size: 3690 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5818 StyI C'CwwG_G Cuts at: 513 1065 2004 2040 3033 3257 513 Size: 552 939 36 993 224 3074 Fragments arranged by size: 3074 993 939 552 224 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2853 3845 3862 4015 4200 4645 325 Size: 1255 1273 992 17 153 185 445 1498 Fragments arranged by size: 1498 1273 1255 992 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4064 5776 96 Size: 274 80 33 51 1443 2087 1712 138 Fragments arranged by size: 2087 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2212 2244 3060 3092 5778 5810 2212 Size: 32 816 32 2686 32 2220 Fragments arranged by size: 2686 2220 816 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5818 XbaI T'CTAG_A Cuts at: 3154 3154 Size: 5818 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5818 XmaI C'CCGG_G Cuts at: 1870 3143 1870 Size: 1273 4545 XmnI GAAnn'nnTTC Cuts at: 3947 3947 Size: 5818 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3626 276 Size: 53 83 186 936 2092 2468 Fragments arranged by size: 2468 2092 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI