(Circular) (Six-base) MAPSORT of: pFN21AB6510.seq Check: 704 from: 1 to: 5857 pFN21AB6510 5857 bp With 182 enzymes: SgfI * October 20, 2009 10:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3667 278 Size: 53 83 186 936 2131 2468 Fragments arranged by size: 2468 2131 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3200 1937 Size: 1263 4594 Acc65I G'GTAC_C Cuts at: 3178 3178 Size: 5857 AclI AA'CG_TT Cuts at: 1563 3984 4357 1563 Size: 2421 373 3063 Fragments arranged by size: 3063 2421 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2063 2564 2691 3920 5304 5572 1632 Size: 431 501 127 1229 1384 268 1917 Fragments arranged by size: 1917 1384 1229 501 431 268 127 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5660 AflIII A'CryG_T Cuts at: 1203 4756 5733 1203 Size: 3553 977 1327 Fragments arranged by size: 3553 1327 977 AgeI A'CCGG_T Cuts at: 4688 4688 Size: 5857 AhdI GACnn_n'nnGTC Cuts at: 4586 4586 Size: 5857 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5857 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2301 2895 1184 Size: 1117 594 4146 Fragments arranged by size: 4146 1117 594 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2679 2711 2679 Size: 32 5825 AlwNI CAG_nnn'CTG Cuts at: 2555 3600 5172 2555 Size: 1045 1572 3240 Fragments arranged by size: 3240 1572 1045 ApaLI G'TGCA_C Cuts at: 2226 3914 5070 2226 Size: 1688 1156 3013 Fragments arranged by size: 3013 1688 1156 ApoI r'AATT_y Cuts at: 1493 2114 2143 3166 3392 3544 5653 1493 Size: 621 29 1023 226 152 2109 1697 Fragments arranged by size: 2109 1697 1023 621 226 152 29 AseI AT'TA_AT Cuts at: 160 1032 4411 160 Size: 872 3379 1606 Fragments arranged by size: 3379 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5857 AvaI C'yCGr_G Cuts at: 1870 1943 3182 1870 Size: 73 1239 4545 Fragments arranged by size: 4545 1239 73 BamHI G'GATC_C Cuts at: 3187 3554 3187 Size: 367 5490 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2972 3178 4633 618 Size: 325 217 627 1185 206 1455 1842 Fragments arranged by size: 1842 1455 1185 627 325 217 206 BanII G_rGCy'C Cuts at: 1391 2112 2985 3176 1391 Size: 721 873 191 4072 Fragments arranged by size: 4072 873 721 191 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4886 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4048 4082 1903 Size: 34 2111 34 3678 Fragments arranged by size: 3678 2111 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3749 4959 3749 Size: 1210 4647 BclI T'GATC_A Cuts at: 1548 1548 Size: 5857 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2372 4468 136 Size: 107 122 71 631 605 700 2096 1525 Fragments arranged by size: 2096 1525 700 631 605 122 107 71 BglII A'GATC_T Cuts at: 2176 3022 5852 2176 Size: 846 2830 2181 Fragments arranged by size: 2830 2181 846 BlpI GC'TnA_GC Cuts at: 2268 3274 2268 Size: 1006 4851 Bme1580I G_kGCm'C Cuts at: 1790 2230 2775 3918 5074 1790 Size: 440 545 1143 1156 2573 Fragments arranged by size: 2573 1156 1143 545 440 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5857 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2329 3588 4546 Size: 416 53 83 399 24 912 1259 958 Cuts at: 4546 442 Size: 1753 Fragments arranged by size: 1753 1259 958 912 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5857 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2294 2756 4517 1239 Size: 1055 462 1761 2579 Fragments arranged by size: 2579 1761 1055 462 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3982 4847 5145 3982 Size: 865 298 4694 Fragments arranged by size: 4694 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4520 882 Size: 617 3021 2219 Fragments arranged by size: 3021 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3648 493 Size: 711 2444 2702 Fragments arranged by size: 2702 2444 711 BsaBI GATnn'nnATC Cuts at: 2688 5851 2688 Size: 3163 2694 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3664 4046 4681 275 Size: 53 83 186 936 2131 382 635 1451 Fragments arranged by size: 2131 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4290 4688 4962 5109 5520 4290 Size: 398 274 147 411 4627 Fragments arranged by size: 4627 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2777 2807 2901 2931 1178 Size: 30 165 30 1374 30 94 30 4104 Fragments arranged by size: 4104 1374 165 94 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2195 2819 2822 1186 Size: 1009 624 3 4221 Fragments arranged by size: 4221 1009 624 3 BseYI C'CCAG_C Cuts at: 5060 5556 5060 Size: 496 5361 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2186 2568 2891 1163 Size: 573 450 382 323 4129 Fragments arranged by size: 4129 573 450 382 323 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3307 4068 5096 1540 Size: 275 186 1306 761 1028 2301 Fragments arranged by size: 2301 1306 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2230 2985 3918 4003 5074 2230 Size: 755 933 85 1071 3013 Fragments arranged by size: 3013 1071 933 755 85 BsmI GAATG_Cn' Cuts at: 3368 3461 3368 Size: 93 5764 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2112 2230 2775 2985 3176 3918 4003 Size: 399 322 118 545 210 191 742 85 Cuts at: 4003 5074 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 545 399 322 210 191 118 85 BspEI T'CCGG_A Cuts at: 5520 5520 Size: 5857 BspHI T'CATG_A Cuts at: 2800 3746 5476 2800 Size: 946 1730 3181 Fragments arranged by size: 3181 1730 946 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2939 3212 844 Size: 2095 273 3489 Fragments arranged by size: 3489 2095 273 BsrBI CCG'CTC Cuts at: 1394 1823 3744 1394 Size: 429 1921 3507 Fragments arranged by size: 3507 1921 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4352 4526 65 Size: 1183 3104 174 1396 Fragments arranged by size: 3104 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4501 4688 1700 Size: 110 2691 187 2869 Fragments arranged by size: 2869 2691 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5857 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5857 BssSI C'ACGA_G Cuts at: 3917 4929 3917 Size: 1012 4845 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5857 BstBI TT'CG_AA Cuts at: 4661 4661 Size: 5857 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5857 BstYI r'GATC_y Cuts at: 1972 2176 2649 3022 3187 3554 3939 3956 5408 Size: 204 473 373 165 367 385 17 1452 Cuts at: 5408 5494 5506 5852 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 473 385 373 367 346 204 165 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2069 513 Size: 552 939 65 4301 Fragments arranged by size: 4301 939 552 65 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5118 Fragments arranged by size: 5118 683 56 BtsI GCAGTG_nn' Cuts at: 3373 4167 4187 3373 Size: 794 20 5043 Fragments arranged by size: 5043 794 20 ClaI AT'CG_AT Cuts at: 1554 2080 1554 Size: 526 5331 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5822 DraI TTT'AAA Cuts at: 3161 3161 Size: 5857 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4864 809 Size: 507 3548 1802 Fragments arranged by size: 3548 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2183 2341 2988 Size: 54 1425 50 275 18 353 158 647 Cuts at: 2988 3111 3304 4193 5822 8 Size: 123 193 889 1629 43 Fragments arranged by size: 1629 1425 889 647 353 275 193 158 123 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3304 1537 Size: 275 1492 4090 Fragments arranged by size: 4090 1492 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2667 3787 1353 Size: 1314 1120 3423 Fragments arranged by size: 3423 1314 1120 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4444 4818 4964 387 Size: 1064 138 2855 374 146 1280 Fragments arranged by size: 2855 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2983 2983 Size: 5857 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2063 2294 2564 2691 2756 3920 4517 Size: 393 431 231 270 127 65 1164 597 Cuts at: 4517 5304 5572 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1164 787 597 431 393 270 268 231 127 65 EcoNI CCTnn'n_nnAGG Cuts at: 2801 2801 Size: 5857 EcoRI G'AATT_C Cuts at: 3166 3544 3166 Size: 378 5479 EcoRV GAT'ATC Cuts at: 2419 2419 Size: 5857 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2891 2891 Size: 5857 FspI TGC'GCA Cuts at: 4363 4363 Size: 5857 HaeII r_GCGC'y Cuts at: 1678 5004 1678 Size: 3326 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2403 2435 2471 2503 2777 Size: 32 135 32 831 32 36 32 274 Cuts at: 2777 2809 4498 4530 4572 4604 1373 Size: 32 1689 32 42 32 2626 Fragments arranged by size: 2626 1689 831 274 135 42 36 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3201 3447 669 Size: 988 281 1263 246 3079 Fragments arranged by size: 3079 1263 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5857 HpaI GTT'AAC Cuts at: 3447 3447 Size: 5857 KpnI G_GTAC'C Cuts at: 3182 3182 Size: 5857 MfeI C'AATT_G Cuts at: 3456 3456 Size: 5857 MluI A'CGCG_T Cuts at: 5733 5733 Size: 5857 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4971 5155 1305 Size: 3666 184 2007 Fragments arranged by size: 3666 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2185 2343 2990 3113 10 Size: 54 1425 343 353 158 647 123 2754 Fragments arranged by size: 2754 1425 647 353 343 158 123 54 MslI CAynn'nnrTG Cuts at: 518 1166 2262 2661 3815 4174 4333 518 Size: 648 1096 399 1154 359 159 2042 Fragments arranged by size: 2042 1154 1096 648 399 359 159 MspA1I CmG'CkG Cuts at: 1606 2071 2281 3273 3950 5098 5343 1606 Size: 465 210 992 677 1148 245 2120 Fragments arranged by size: 2120 1148 992 677 465 245 210 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4366 Fragments arranged by size: 4366 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5857 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5857 NotI GC'GGCC_GC Cuts at: 3304 3304 Size: 5857 NruI TCG'CGA Cuts at: 2060 2060 Size: 5857 NspI r_CATG'y Cuts at: 1132 1229 3215 4760 1132 Size: 97 1986 1545 2229 Fragments arranged by size: 2229 1986 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5857 PciI A'CATG_T Cuts at: 4756 4756 Size: 5857 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5857 PfoI T'CCnGG_A Cuts at: 2031 2031 Size: 5857 PmeI GTTT'AAAC Cuts at: 3161 3161 Size: 5857 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3926 3958 5439 5471 3926 Size: 32 1481 32 4312 Fragments arranged by size: 4312 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5857 PsiI TTA'TAA Cuts at: 3427 3427 Size: 5857 PstI C_TGCA'G Cuts at: 830 2331 2353 2381 2520 3209 830 Size: 1501 22 28 139 689 3478 Fragments arranged by size: 3478 1501 689 139 28 22 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5857 PvuII CAG'CTG Cuts at: 2281 2281 Size: 5857 SacI G_AGCT'C Cuts at: 2985 2985 Size: 5857 SacII CC_GC'GG Cuts at: 2072 2072 Size: 5857 SalI G'TCGA_C Cuts at: 1936 3199 1936 Size: 1263 4594 SbfI CC_TGCA'GG Cuts at: 3209 3209 Size: 5857 ScaI AGT'ACT Cuts at: 2468 4105 5817 2468 Size: 1637 1712 2508 Fragments arranged by size: 2508 1712 1637 SfcI C'TryA_G Cuts at: 826 1045 2327 2349 2377 2516 2642 3205 4340 Size: 219 1282 22 28 139 126 563 1135 Cuts at: 4340 5021 5212 826 Size: 681 191 1471 Fragments arranged by size: 1471 1282 1135 681 563 219 191 139 126 28 22 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5857 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5857 SmaI CCC'GGG Cuts at: 1872 3184 1872 Size: 1312 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3961 4862 5124 820 Size: 197 926 2018 901 262 1553 Fragments arranged by size: 2018 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3648 493 Size: 3155 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5857 SphI G_CATG'C Cuts at: 1132 3215 1132 Size: 2083 3774 SspI AAT'ATT Cuts at: 5 52 3781 5 Size: 47 3729 2081 Fragments arranged by size: 3729 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5857 StyI C'CwwG_G Cuts at: 513 1065 2004 2289 3296 513 Size: 552 939 285 1007 3074 Fragments arranged by size: 3074 1007 939 552 285 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2444 3884 3901 4054 4239 4684 325 Size: 1255 864 1440 17 153 185 445 1498 Fragments arranged by size: 1498 1440 1255 864 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2466 3129 4103 Size: 274 80 33 51 1443 489 663 974 Cuts at: 4103 5815 96 Size: 1712 138 Fragments arranged by size: 1712 1443 974 663 489 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5817 5849 5817 Size: 32 5825 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5857 XbaI T'CTAG_A Cuts at: 3193 3193 Size: 5857 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2983 2983 Size: 5857 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5857 XmaI C'CCGG_G Cuts at: 1870 3182 1870 Size: 1312 4545 XmnI GAAnn'nnTTC Cuts at: 3986 3986 Size: 5857 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3665 276 Size: 53 83 186 936 2131 2468 Fragments arranged by size: 2468 2131 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI