(Circular) (Six-base) MAPSORT of: pFN21AB6505.seq Check: 384 from: 1 to: 5779 pFN21AB6505 5779 bp With 182 enzymes: SgfI * October 20, 2009 10:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3589 278 Size: 53 83 186 936 2053 2468 Fragments arranged by size: 2468 2053 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2285 3122 1937 Size: 348 837 4594 Fragments arranged by size: 4594 837 348 Acc65I G'GTAC_C Cuts at: 2390 3100 2390 Size: 710 5069 AclI AA'CG_TT Cuts at: 1563 3906 4279 1563 Size: 2343 373 3063 Fragments arranged by size: 3063 2343 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3842 5226 5494 1632 Size: 2210 1384 268 1917 Fragments arranged by size: 2210 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5582 AflIII A'CryG_T Cuts at: 1203 2664 4678 5655 1203 Size: 1461 2014 977 1327 Fragments arranged by size: 2014 1461 1327 977 AgeI A'CCGG_T Cuts at: 4610 4610 Size: 5779 AhdI GACnn_n'nnGTC Cuts at: 4508 4508 Size: 5779 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5779 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2951 1184 Size: 1767 4012 AlwNI CAG_nnn'CTG Cuts at: 2260 2279 3522 5094 2260 Size: 19 1243 1572 2945 Fragments arranged by size: 2945 1572 1243 19 ApaI G_GGCC'C Cuts at: 2121 2214 2121 Size: 93 5686 ApaLI G'TGCA_C Cuts at: 3836 4992 3836 Size: 1156 4623 ApoI r'AATT_y Cuts at: 1493 3088 3314 3466 5575 1493 Size: 1595 226 152 2109 1697 Fragments arranged by size: 2109 1697 1595 226 152 AseI AT'TA_AT Cuts at: 160 1032 4333 160 Size: 872 3301 1606 Fragments arranged by size: 3301 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5779 AvaI C'yCGr_G Cuts at: 1870 1943 2231 2716 3104 1870 Size: 73 288 485 388 4545 Fragments arranged by size: 4545 485 388 288 73 BamHI G'GATC_C Cuts at: 3109 3476 3109 Size: 367 5412 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2390 3100 4555 618 Size: 325 217 627 603 710 1455 1842 Fragments arranged by size: 1842 1455 710 627 603 325 217 BanII G_rGCy'C Cuts at: 1391 2110 2121 2159 2214 2232 2334 3098 1391 Size: 719 11 38 55 18 102 764 4072 Fragments arranged by size: 4072 764 719 102 55 38 18 11 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2563 928 Size: 971 664 4144 Fragments arranged by size: 4144 971 664 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2348 2382 2838 2872 3970 Size: 34 50 34 327 34 456 34 1098 Cuts at: 3970 4004 1903 Size: 34 3678 Fragments arranged by size: 3678 1098 456 327 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2474 3671 4881 2474 Size: 1197 1210 3372 Fragments arranged by size: 3372 1210 1197 BclI T'GATC_A Cuts at: 1548 1548 Size: 5779 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2255 2274 4390 Size: 107 122 71 631 605 583 19 2116 Cuts at: 4390 136 Size: 1525 Fragments arranged by size: 2116 1525 631 605 583 122 107 71 19 BglII A'GATC_T Cuts at: 5774 5774 Size: 5779 BlpI GC'TnA_GC Cuts at: 3196 3196 Size: 5779 Bme1580I G_kGCm'C Cuts at: 1790 2121 2214 2672 3840 4996 1790 Size: 331 93 458 1168 1156 2573 Fragments arranged by size: 2573 1168 1156 458 331 93 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5779 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2643 3032 3510 Size: 416 53 83 399 24 1226 389 478 Cuts at: 3510 4468 442 Size: 958 1753 Fragments arranged by size: 1753 1226 958 478 416 399 389 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5779 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2154 2246 2348 2624 2633 2758 4439 1239 Size: 915 92 102 276 9 125 1681 2579 Fragments arranged by size: 2579 1681 915 276 125 102 92 9 Bpu10I CC'TnA_GC Cuts at: 2153 2469 2968 2153 Size: 316 499 4964 Fragments arranged by size: 4964 499 316 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2537 3904 4769 5067 2537 Size: 1367 865 298 3249 Fragments arranged by size: 3249 1367 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4442 882 Size: 617 2943 2219 Fragments arranged by size: 2943 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2680 3570 493 Size: 711 1476 890 2702 Fragments arranged by size: 2702 1476 890 711 BsaBI GATnn'nnATC Cuts at: 5773 5773 Size: 5779 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3586 3968 4603 275 Size: 53 83 186 936 2053 382 635 1451 Fragments arranged by size: 2053 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4212 4610 4884 5031 5442 4212 Size: 398 274 147 411 4549 Fragments arranged by size: 4549 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5554 Fragments arranged by size: 5554 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2249 2390 3062 1186 Size: 1063 141 672 3903 Fragments arranged by size: 3903 1063 672 141 BseYI C'CCAG_C Cuts at: 2121 2394 2671 4982 5478 2121 Size: 273 277 2311 496 2422 Fragments arranged by size: 2422 2311 496 277 273 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2246 2921 1163 Size: 573 510 675 4021 Fragments arranged by size: 4021 675 573 510 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3229 3990 5018 1540 Size: 275 186 1228 761 1028 2301 Fragments arranged by size: 2301 1228 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2110 2490 3840 3925 4996 2110 Size: 380 1350 85 1071 2893 Fragments arranged by size: 2893 1350 1071 380 85 BsmI GAATG_Cn' Cuts at: 3290 3383 3290 Size: 93 5686 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2110 2121 2159 2214 2232 2334 2490 Size: 399 320 11 38 55 18 102 156 Cuts at: 2490 2672 3098 3840 3925 4996 1391 Size: 182 426 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 426 399 320 182 156 102 85 55 38 18 11 BspEI T'CCGG_A Cuts at: 5442 5442 Size: 5779 BspHI T'CATG_A Cuts at: 3668 5398 3668 Size: 1730 4049 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2297 3134 844 Size: 1453 837 3489 Fragments arranged by size: 3489 1453 837 BsrBI CCG'CTC Cuts at: 1394 1823 3666 1394 Size: 429 1843 3507 Fragments arranged by size: 3507 1843 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4274 4448 65 Size: 1183 3026 174 1396 Fragments arranged by size: 3026 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2771 4423 4610 1700 Size: 110 961 1652 187 2869 Fragments arranged by size: 2869 1652 961 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5779 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5779 BssSI C'ACGA_G Cuts at: 3839 4851 3839 Size: 1012 4767 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5779 BstBI TT'CG_AA Cuts at: 4583 4583 Size: 5779 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5779 BstXI CCAn_nnnn'nTGG Cuts at: 2020 2512 2020 Size: 492 5287 BstYI r'GATC_y Cuts at: 1972 2820 3109 3476 3861 3878 5330 5416 5428 Size: 848 289 367 385 17 1452 86 12 Cuts at: 5428 5774 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 848 385 367 346 289 86 17 12 BstZ17I GTA'TAC Cuts at: 2286 2286 Size: 5779 Bsu36I CC'TnA_GG Cuts at: 2475 2475 Size: 5779 BtgI C'CryG_G Cuts at: 513 1065 2425 2827 513 Size: 552 1360 402 3465 Fragments arranged by size: 3465 1360 552 402 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5040 Fragments arranged by size: 5040 683 56 BtsI GCAGTG_nn' Cuts at: 2193 2263 3295 4089 4109 2193 Size: 70 1032 794 20 3863 Fragments arranged by size: 3863 1032 794 70 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5779 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2169 2204 529 Size: 35 1605 35 4104 Fragments arranged by size: 4104 1605 35 35 DraI TTT'AAA Cuts at: 3083 3083 Size: 5779 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4786 809 Size: 507 3470 1802 Fragments arranged by size: 3470 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2190 2319 2502 Size: 54 1425 50 275 18 360 129 183 Cuts at: 2502 2769 2773 3226 4115 5744 8 Size: 267 4 453 889 1629 43 Fragments arranged by size: 1629 1425 889 453 360 275 267 183 129 54 50 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 3226 1537 Size: 275 1414 4090 Fragments arranged by size: 4090 1414 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2495 2762 2816 3039 3709 1353 Size: 1142 267 54 223 670 3423 Fragments arranged by size: 3423 1142 670 267 223 54 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2483 2813 4366 4740 4886 387 Size: 1064 138 894 330 1553 374 146 1280 Fragments arranged by size: 1553 1280 1064 894 374 330 146 138 EcoICRI GAG'CTC Cuts at: 2108 2108 Size: 5779 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2154 2246 2348 2624 2633 2758 3842 Size: 393 522 92 102 276 9 125 1084 Cuts at: 3842 4439 5226 5494 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1084 787 597 522 393 276 268 125 102 92 9 EcoO109I rG'GnC_Cy Cuts at: 2118 2211 2257 2380 2684 2832 2956 2118 Size: 93 46 123 304 148 124 4941 Fragments arranged by size: 4941 304 148 124 123 93 46 EcoRI G'AATT_C Cuts at: 3088 3466 3088 Size: 378 5401 FseI GG_CCGG'CC Cuts at: 2775 2775 Size: 5779 FspI TGC'GCA Cuts at: 4285 4285 Size: 5779 HaeII r_GCGC'y Cuts at: 1678 2756 4926 1678 Size: 1078 2170 2531 Fragments arranged by size: 2531 2170 1078 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2973 3005 4420 4452 4494 Size: 32 135 32 1401 32 1415 32 42 Cuts at: 4494 4526 1373 Size: 32 2626 Fragments arranged by size: 2626 1415 1401 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3123 3369 669 Size: 988 281 1185 246 3079 Fragments arranged by size: 3079 1185 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5779 HpaI GTT'AAC Cuts at: 3369 3369 Size: 5779 KpnI G_GTAC'C Cuts at: 2394 3104 2394 Size: 710 5069 MfeI C'AATT_G Cuts at: 3378 3378 Size: 5779 MluI A'CGCG_T Cuts at: 2664 5655 2664 Size: 2991 2788 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2040 2046 4893 5077 1305 Size: 735 6 2847 184 2007 Fragments arranged by size: 2847 2007 735 184 6 MscI TGG'CCA Cuts at: 10 64 1489 1832 2192 2504 10 Size: 54 1425 343 360 312 3285 Fragments arranged by size: 3285 1425 360 343 312 54 MslI CAynn'nnrTG Cuts at: 518 1166 2358 3737 4096 4255 518 Size: 648 1192 1379 359 159 2042 Fragments arranged by size: 2042 1379 1192 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2829 2871 2906 3195 3872 5020 5265 1606 Size: 1223 42 35 289 677 1148 245 2120 Fragments arranged by size: 2120 1223 1148 677 289 245 42 35 NaeI GCC'GGC Cuts at: 2773 2773 Size: 5779 NcoI C'CATG_G Cuts at: 513 1065 2425 513 Size: 552 1360 3867 Fragments arranged by size: 3867 1360 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5779 NgoMIV G'CCGG_C Cuts at: 2771 2771 Size: 5779 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5779 NotI GC'GGCC_GC Cuts at: 3226 3226 Size: 5779 NspI r_CATG'y Cuts at: 1132 1229 2363 2437 3137 4682 1132 Size: 97 1134 74 700 1545 2229 Fragments arranged by size: 2229 1545 1134 700 97 74 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5779 PciI A'CATG_T Cuts at: 4678 4678 Size: 5779 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5779 PfoI T'CCnGG_A Cuts at: 2045 2223 2307 2787 2045 Size: 178 84 480 5037 Fragments arranged by size: 5037 480 178 84 PmeI GTTT'AAAC Cuts at: 3083 3083 Size: 5779 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3051 3083 3848 3880 5361 5393 3051 Size: 32 765 32 1481 32 3437 Fragments arranged by size: 3437 1481 765 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2380 2684 2956 2380 Size: 304 272 5203 Fragments arranged by size: 5203 304 272 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5779 PsiI TTA'TAA Cuts at: 3349 3349 Size: 5779 PspOMI G'GGCC_C Cuts at: 2117 2210 2117 Size: 93 5686 PstI C_TGCA'G Cuts at: 830 2851 3131 830 Size: 2021 280 3478 Fragments arranged by size: 3478 2021 280 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5779 PvuII CAG'CTG Cuts at: 2906 2906 Size: 5779 SacI G_AGCT'C Cuts at: 2110 2110 Size: 5779 SacII CC_GC'GG Cuts at: 2830 2830 Size: 5779 SalI G'TCGA_C Cuts at: 1936 3121 1936 Size: 1185 4594 SapI GCTCTTCn'nnn_ Cuts at: 2495 2762 2816 2495 Size: 267 54 5458 Fragments arranged by size: 5458 267 54 SbfI CC_TGCA'GG Cuts at: 2851 3131 2851 Size: 280 5499 ScaI AGT'ACT Cuts at: 4027 5739 4027 Size: 1712 4067 SfcI C'TryA_G Cuts at: 826 1045 2271 2847 3127 4262 4943 5134 826 Size: 219 1226 576 280 1135 681 191 1471 Fragments arranged by size: 1471 1226 1135 681 576 280 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2255 2255 Size: 5779 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5779 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5779 SmaI CCC'GGG Cuts at: 1872 3106 1872 Size: 1234 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2552 3883 4784 5046 820 Size: 197 926 609 1331 901 262 1553 Fragments arranged by size: 1553 1331 926 901 609 262 197 SnaBI TAC'GTA Cuts at: 493 3570 493 Size: 3077 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5779 SphI G_CATG'C Cuts at: 1132 3137 1132 Size: 2005 3774 SspI AAT'ATT Cuts at: 5 52 3703 5 Size: 47 3651 2081 Fragments arranged by size: 3651 2081 47 StuI AGG'CCT Cuts at: 1517 2853 1517 Size: 1336 4443 StyI C'CwwG_G Cuts at: 513 1065 2425 3218 513 Size: 552 1360 793 3074 Fragments arranged by size: 3074 1360 793 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3806 3823 3976 4161 4606 325 Size: 1255 2226 17 153 185 445 1498 Fragments arranged by size: 2226 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4025 5737 96 Size: 274 80 33 51 1443 2048 1712 138 Fragments arranged by size: 2048 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5739 5771 5739 Size: 32 5747 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5779 XbaI T'CTAG_A Cuts at: 3115 3115 Size: 5779 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2380 2380 Size: 5779 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5779 XmaI C'CCGG_G Cuts at: 1870 3104 1870 Size: 1234 4545 XmnI GAAnn'nnTTC Cuts at: 3066 3908 3066 Size: 842 4937 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3587 276 Size: 53 83 186 936 2053 2468 Fragments arranged by size: 2468 2053 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI DraIII EcoNI EcoRV FalI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI SexAI SfoI SrfI SwaI