(Circular) (Six-base) MAPSORT of: pFN21AB6418.seq Check: 7523 from: 1 to: 5716 pFN21AB6418 5716 bp With 182 enzymes: SgfI * October 20, 2009 10:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3526 278 Size: 53 83 186 936 1990 2468 Fragments arranged by size: 2468 1990 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2325 3059 1937 Size: 388 734 4594 Fragments arranged by size: 4594 734 388 Acc65I G'GTAC_C Cuts at: 3037 3037 Size: 5716 AclI AA'CG_TT Cuts at: 1563 3843 4216 1563 Size: 2280 373 3063 Fragments arranged by size: 3063 2280 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3779 5163 5431 1632 Size: 2147 1384 268 1917 Fragments arranged by size: 2147 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5519 AflIII A'CryG_T Cuts at: 1203 4615 5592 1203 Size: 3412 977 1327 Fragments arranged by size: 3412 1327 977 AgeI A'CCGG_T Cuts at: 4547 4547 Size: 5716 AhdI GACnn_n'nnGTC Cuts at: 4445 4445 Size: 5716 AleI CACnn'nnGTG Cuts at: 1166 2072 2075 1166 Size: 906 3 4807 Fragments arranged by size: 4807 906 3 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5716 AlwNI CAG_nnn'CTG Cuts at: 2185 3459 5031 2185 Size: 1274 1572 2870 Fragments arranged by size: 2870 1572 1274 ApaLI G'TGCA_C Cuts at: 3773 4929 3773 Size: 1156 4560 ApoI r'AATT_y Cuts at: 1493 2418 2451 3025 3251 3403 5512 1493 Size: 925 33 574 226 152 2109 1697 Fragments arranged by size: 2109 1697 925 574 226 152 33 AseI AT'TA_AT Cuts at: 160 1032 4270 160 Size: 872 3238 1606 Fragments arranged by size: 3238 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5716 AvaI C'yCGr_G Cuts at: 1870 1943 2471 3041 1870 Size: 73 528 570 4545 Fragments arranged by size: 4545 570 528 73 BamHI G'GATC_C Cuts at: 2937 3046 3413 2937 Size: 109 367 5240 Fragments arranged by size: 5240 367 109 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2708 2815 3037 4492 618 Size: 325 217 627 921 107 222 1455 1842 Fragments arranged by size: 1842 1455 921 627 325 222 217 107 BanII G_rGCy'C Cuts at: 1391 2015 2388 2472 2628 3035 1391 Size: 624 373 84 156 407 4072 Fragments arranged by size: 4072 624 407 373 156 84 BbeI G_GCGC'C Cuts at: 2712 2712 Size: 5716 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2640 928 Size: 971 741 4004 Fragments arranged by size: 4004 971 741 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3907 3941 1903 Size: 34 1970 34 3678 Fragments arranged by size: 3678 1970 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2323 3608 4818 2323 Size: 1285 1210 3221 Fragments arranged by size: 3221 1285 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5716 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2837 4327 136 Size: 107 122 71 631 605 1165 1490 1525 Fragments arranged by size: 1525 1490 1165 631 605 122 107 71 BglII A'GATC_T Cuts at: 5711 5711 Size: 5716 BlpI GC'TnA_GC Cuts at: 3133 3133 Size: 5716 Bme1580I G_kGCm'C Cuts at: 1790 3777 4933 1790 Size: 1987 1156 2573 Fragments arranged by size: 2573 1987 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5716 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3447 4405 442 Size: 416 53 83 399 24 2030 958 1753 Fragments arranged by size: 2030 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5716 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2944 2944 Size: 5716 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2990 4376 1239 Size: 1751 1386 2579 Fragments arranged by size: 2579 1751 1386 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2042 3841 4706 5004 2042 Size: 1799 865 298 2754 Fragments arranged by size: 2754 1799 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4379 882 Size: 617 2880 2219 Fragments arranged by size: 2880 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3507 493 Size: 711 2303 2702 Fragments arranged by size: 2702 2303 711 BsaBI GATnn'nnATC Cuts at: 5710 5710 Size: 5716 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2709 3523 3905 4540 Size: 53 83 186 936 1176 814 382 635 Cuts at: 4540 275 Size: 1451 Fragments arranged by size: 1451 1176 936 814 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4149 4547 4821 4968 5379 4149 Size: 398 274 147 411 4486 Fragments arranged by size: 4486 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2763 2793 1178 Size: 30 165 30 1360 30 4101 Fragments arranged by size: 4101 1360 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2207 2489 1186 Size: 1021 282 4413 Fragments arranged by size: 4413 1021 282 BseYI C'CCAG_C Cuts at: 2218 2399 4919 5415 2218 Size: 181 2520 496 2519 Fragments arranged by size: 2520 2519 496 181 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5143 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3166 3927 4955 1540 Size: 275 186 1165 761 1028 2301 Fragments arranged by size: 2301 1165 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2015 2388 2472 2826 3777 3862 4933 2015 Size: 373 84 354 951 85 1071 2798 Fragments arranged by size: 2798 1071 951 373 354 85 84 BsmI GAATG_Cn' Cuts at: 3227 3320 3227 Size: 93 5623 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2015 2388 2472 2628 2826 3035 3777 Size: 399 225 373 84 156 198 209 742 Cuts at: 3777 3862 4933 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 373 225 209 198 156 85 84 BspEI T'CCGG_A Cuts at: 5379 5379 Size: 5716 BspHI T'CATG_A Cuts at: 3605 5335 3605 Size: 1730 3986 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3071 844 Size: 2227 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2974 3603 1394 Size: 429 1151 629 3507 Fragments arranged by size: 3507 1151 629 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4211 4385 65 Size: 1183 2963 174 1396 Fragments arranged by size: 2963 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2747 4360 4547 1700 Size: 110 937 1613 187 2869 Fragments arranged by size: 2869 1613 937 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5716 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5716 BssSI C'ACGA_G Cuts at: 3776 4788 3776 Size: 1012 4704 BstAPI GCAn_nnn'nTGC Cuts at: 794 2493 794 Size: 1699 4017 BstBI TT'CG_AA Cuts at: 4520 4520 Size: 5716 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5716 BstXI CCAn_nnnn'nTGG Cuts at: 2077 2704 2782 2854 2077 Size: 627 78 72 4939 Fragments arranged by size: 4939 627 78 72 BstYI r'GATC_y Cuts at: 1972 2937 3046 3413 3798 3815 5267 5353 5365 Size: 965 109 367 385 17 1452 86 12 Cuts at: 5365 5711 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 965 385 367 346 109 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2073 513 Size: 552 1008 4156 Fragments arranged by size: 4156 1008 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2369 487 Size: 683 56 1143 3834 Fragments arranged by size: 3834 1143 683 56 BtsI GCAGTG_nn' Cuts at: 2491 2967 3232 4026 4046 2491 Size: 476 265 794 20 4161 Fragments arranged by size: 4161 794 476 265 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5716 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5681 DraI TTT'AAA Cuts at: 3020 3020 Size: 5716 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4723 809 Size: 507 3407 1802 Fragments arranged by size: 3407 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2079 2154 2209 Size: 54 1425 50 275 18 249 75 55 Cuts at: 2209 2361 2745 3163 4052 5681 8 Size: 152 384 418 889 1629 43 Fragments arranged by size: 1629 1425 889 418 384 275 249 152 75 55 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3163 1537 Size: 275 1351 4090 Fragments arranged by size: 4090 1351 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2516 3646 1353 Size: 1163 1130 3423 Fragments arranged by size: 3423 1163 1130 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4303 4677 4823 387 Size: 1064 138 2714 374 146 1280 Fragments arranged by size: 2714 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2013 2386 2470 2013 Size: 373 84 5259 Fragments arranged by size: 5259 373 84 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2990 3779 4376 5163 5431 1239 Size: 393 1358 789 597 787 268 1524 Fragments arranged by size: 1524 1358 789 787 597 393 268 EcoRI G'AATT_C Cuts at: 2418 2451 3025 3403 2418 Size: 33 574 378 4731 Fragments arranged by size: 4731 574 378 33 FspI TGC'GCA Cuts at: 4222 4222 Size: 5716 HaeII r_GCGC'y Cuts at: 1678 2712 4863 1678 Size: 1034 2151 2531 Fragments arranged by size: 2531 2151 1034 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2600 2632 2864 2896 4357 Size: 32 135 32 1028 32 232 32 1461 Cuts at: 4357 4389 4431 4463 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1461 1028 232 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3060 3306 669 Size: 988 281 1122 246 3079 Fragments arranged by size: 3079 1122 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5716 HpaI GTT'AAC Cuts at: 3306 3306 Size: 5716 KasI G'GCGC_C Cuts at: 2708 2708 Size: 5716 KpnI G_GTAC'C Cuts at: 3041 3041 Size: 5716 MfeI C'AATT_G Cuts at: 3315 3315 Size: 5716 MluI A'CGCG_T Cuts at: 5592 5592 Size: 5716 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4830 5014 1305 Size: 3525 184 2007 Fragments arranged by size: 3525 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2363 10 Size: 54 1425 343 531 3363 Fragments arranged by size: 3363 1425 531 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2072 2075 2702 2871 3674 4033 4192 Size: 648 906 3 627 169 803 359 159 Cuts at: 4192 518 Size: 2042 Fragments arranged by size: 2042 906 803 648 627 359 169 159 3 MspA1I CmG'CkG Cuts at: 1606 2954 3132 3809 4957 5202 1606 Size: 1348 178 677 1148 245 2120 Fragments arranged by size: 2120 1348 1148 677 245 178 NaeI GCC'GGC Cuts at: 2749 2749 Size: 5716 NarI GG'CG_CC Cuts at: 2709 2709 Size: 5716 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5164 NdeI CA'TA_TG Cuts at: 387 387 Size: 5716 NgoMIV G'CCGG_C Cuts at: 2747 2747 Size: 5716 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5716 NotI GC'GGCC_GC Cuts at: 3163 3163 Size: 5716 NspI r_CATG'y Cuts at: 1132 1229 2597 3074 4619 1132 Size: 97 1368 477 1545 2229 Fragments arranged by size: 2229 1545 1368 477 97 PasI CC'CwG_GG Cuts at: 1348 2639 1348 Size: 1291 4425 PciI A'CATG_T Cuts at: 4615 4615 Size: 5716 PflMI CCAn_nnn'nTGG Cuts at: 1108 2185 2530 2573 1108 Size: 1077 345 43 4251 Fragments arranged by size: 4251 1077 345 43 PmeI GTTT'AAAC Cuts at: 3020 3020 Size: 5716 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3785 3817 5298 5330 3785 Size: 32 1481 32 4171 Fragments arranged by size: 4171 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5716 PsiI TTA'TAA Cuts at: 2426 3286 2426 Size: 860 4856 PspXI vC'TCGA_Gb Cuts at: 2471 2471 Size: 5716 PstI C_TGCA'G Cuts at: 830 2799 3068 830 Size: 1969 269 3478 Fragments arranged by size: 3478 1969 269 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5716 SacI G_AGCT'C Cuts at: 2015 2388 2472 2015 Size: 373 84 5259 Fragments arranged by size: 5259 373 84 SalI G'TCGA_C Cuts at: 1936 3058 1936 Size: 1122 4594 SbfI CC_TGCA'GG Cuts at: 3068 3068 Size: 5716 ScaI AGT'ACT Cuts at: 3964 5676 3964 Size: 1712 4004 SfcI C'TryA_G Cuts at: 826 1045 2795 3064 4199 4880 5071 826 Size: 219 1750 269 1135 681 191 1471 Fragments arranged by size: 1750 1471 1135 681 269 219 191 SfoI GGC'GCC Cuts at: 2710 2710 Size: 5716 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5716 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5716 SmaI CCC'GGG Cuts at: 1872 3043 1872 Size: 1171 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2057 2471 3820 4721 4983 820 Size: 197 926 114 414 1349 901 262 1553 Fragments arranged by size: 1553 1349 926 901 414 262 197 114 SnaBI TAC'GTA Cuts at: 493 3507 493 Size: 3014 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5716 SphI G_CATG'C Cuts at: 1132 3074 1132 Size: 1942 3774 SspI AAT'ATT Cuts at: 5 52 3640 5 Size: 47 3588 2081 Fragments arranged by size: 3588 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5716 StyI C'CwwG_G Cuts at: 513 1065 2832 3155 513 Size: 552 1767 323 3074 Fragments arranged by size: 3074 1767 552 323 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2430 2788 3743 3760 3913 4098 4543 Size: 1255 850 358 955 17 153 185 445 Cuts at: 4543 325 Size: 1498 Fragments arranged by size: 1498 1255 955 850 445 358 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2112 3962 5674 Size: 274 80 33 51 1443 135 1850 1712 Cuts at: 5674 96 Size: 138 Fragments arranged by size: 1850 1712 1443 274 138 135 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2763 2795 5676 5708 2763 Size: 32 2881 32 2771 Fragments arranged by size: 2881 2771 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5716 XbaI T'CTAG_A Cuts at: 3052 3052 Size: 5716 XhoI C'TCGA_G Cuts at: 1943 2471 1943 Size: 528 5188 XmaI C'CCGG_G Cuts at: 1870 3041 1870 Size: 1171 4545 XmnI GAAnn'nnTTC Cuts at: 2451 2882 3845 2451 Size: 431 963 4322 Fragments arranged by size: 4322 963 431 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3524 276 Size: 53 83 186 936 1990 2468 Fragments arranged by size: 2468 1990 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI