(Circular) (Six-base) MAPSORT of: pFN21AB6504.seq Check: 10 from: 1 to: 5851 pFN21AB6504 5851 bp With 182 enzymes: SgfI * October 20, 2009 10:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3661 278 Size: 53 83 186 936 2125 2468 Fragments arranged by size: 2468 2125 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3194 1937 Size: 1257 4594 Acc65I G'GTAC_C Cuts at: 3010 3172 3010 Size: 162 5689 AclI AA'CG_TT Cuts at: 1563 3978 4351 1563 Size: 2415 373 3063 Fragments arranged by size: 3063 2415 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2708 3914 5298 5566 1632 Size: 1076 1206 1384 268 1917 Fragments arranged by size: 1917 1384 1206 1076 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5654 AflIII A'CryG_T Cuts at: 1203 4750 5727 1203 Size: 3547 977 1327 Fragments arranged by size: 3547 1327 977 AgeI A'CCGG_T Cuts at: 4682 4682 Size: 5851 AhdI GACnn_n'nnGTC Cuts at: 4580 4580 Size: 5851 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5851 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2312 3078 1184 Size: 1128 766 3957 Fragments arranged by size: 3957 1128 766 AlwNI CAG_nnn'CTG Cuts at: 3594 5166 3594 Size: 1572 4279 ApaLI G'TGCA_C Cuts at: 3908 5064 3908 Size: 1156 4695 ApoI r'AATT_y Cuts at: 1493 3160 3386 3538 5647 1493 Size: 1667 226 152 2109 1697 Fragments arranged by size: 2109 1697 1667 226 152 AseI AT'TA_AT Cuts at: 160 1032 4405 160 Size: 872 3373 1606 Fragments arranged by size: 3373 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5851 AvaI C'yCGr_G Cuts at: 1870 1943 2323 2332 2954 3176 1870 Size: 73 380 9 622 222 4545 Fragments arranged by size: 4545 622 380 222 73 9 BamHI G'GATC_C Cuts at: 2269 2407 2778 3181 3548 2269 Size: 138 371 403 367 4572 Fragments arranged by size: 4572 403 371 367 138 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2169 2727 3010 3172 4627 Size: 325 217 627 382 558 283 162 1455 Cuts at: 4627 618 Size: 1842 Fragments arranged by size: 1842 1455 627 558 382 325 283 217 162 BanII G_rGCy'C Cuts at: 1391 2141 2796 2824 2964 3150 3170 1391 Size: 750 655 28 140 186 20 4072 Fragments arranged by size: 4072 750 655 186 140 28 20 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2985 928 Size: 971 1086 3794 Fragments arranged by size: 3794 1086 971 BbvCI CC'TCA_GC Cuts at: 2879 2879 Size: 5851 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4042 4076 1903 Size: 34 50 34 2021 34 3678 Fragments arranged by size: 3678 2021 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3743 4953 3743 Size: 1210 4641 BclI T'GATC_A Cuts at: 1548 1548 Size: 5851 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4462 136 Size: 107 122 71 631 605 2790 1525 Fragments arranged by size: 2790 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5846 5846 Size: 5851 BlpI GC'TnA_GC Cuts at: 2618 3268 2618 Size: 650 5201 Bme1580I G_kGCm'C Cuts at: 1790 2574 2581 3912 5068 1790 Size: 784 7 1331 1156 2573 Fragments arranged by size: 2573 1331 1156 784 7 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5851 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3582 4540 442 Size: 416 53 83 399 24 2165 958 1753 Fragments arranged by size: 2165 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5851 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2305 2381 2609 2633 4511 1239 Size: 1066 76 228 24 1878 2579 Fragments arranged by size: 2579 1878 1066 228 76 24 Bpu10I CC'TnA_GC Cuts at: 2879 2879 Size: 5851 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3976 4841 5139 3976 Size: 865 298 4688 Fragments arranged by size: 4688 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4514 882 Size: 617 3015 2219 Fragments arranged by size: 3015 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2398 3642 493 Size: 711 1194 1244 2702 Fragments arranged by size: 2702 1244 1194 711 BsaBI GATnn'nnATC Cuts at: 5845 5845 Size: 5851 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3658 4040 4675 275 Size: 53 83 186 936 2125 382 635 1451 Fragments arranged by size: 2125 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4284 4682 4956 5103 5514 4284 Size: 398 274 147 411 4621 Fragments arranged by size: 4621 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3003 3033 1178 Size: 30 165 30 1600 30 3996 Fragments arranged by size: 3996 1600 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2198 2896 1186 Size: 1012 698 4141 Fragments arranged by size: 4141 1012 698 BseYI C'CCAG_C Cuts at: 2041 2194 2736 5054 5550 2041 Size: 153 542 2318 496 2342 Fragments arranged by size: 2342 2318 542 496 153 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2885 1163 Size: 573 1149 4129 Fragments arranged by size: 4129 1149 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3301 4062 5090 1540 Size: 275 186 1300 761 1028 2301 Fragments arranged by size: 2301 1300 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2291 2796 3021 3912 3997 5068 2291 Size: 505 225 891 85 1071 3074 Fragments arranged by size: 3074 1071 891 505 225 85 BsmI GAATG_Cn' Cuts at: 3114 3362 3455 3114 Size: 248 93 5510 Fragments arranged by size: 5510 248 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2285 2285 Size: 5851 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2141 2291 2574 2581 2796 2824 2964 Size: 399 351 150 283 7 215 28 140 Cuts at: 2964 3021 3150 3170 3912 3997 5068 1391 Size: 57 129 20 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 351 283 215 150 140 129 85 57 28 20 7 BspEI T'CCGG_A Cuts at: 5514 5514 Size: 5851 BspHI T'CATG_A Cuts at: 3740 5470 3740 Size: 1730 4121 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2378 2922 3206 844 Size: 1534 544 284 3489 Fragments arranged by size: 3489 1534 544 284 BsrBI CCG'CTC Cuts at: 1394 1823 2163 3738 1394 Size: 429 340 1575 3507 Fragments arranged by size: 3507 1575 429 340 BsrDI GCAATG_nn' Cuts at: 65 1248 2458 4346 4520 65 Size: 1183 1210 1888 174 1396 Fragments arranged by size: 1888 1396 1210 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4495 4682 1700 Size: 110 2685 187 2869 Fragments arranged by size: 2869 2685 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5851 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5851 BssSI C'ACGA_G Cuts at: 3911 4923 3911 Size: 1012 4839 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5851 BstBI TT'CG_AA Cuts at: 4655 4655 Size: 5851 BstEII G'GTnAC_C Cuts at: 1184 2647 1184 Size: 1463 4388 BstYI r'GATC_y Cuts at: 1972 2269 2407 2778 3181 3548 3933 3950 5402 Size: 297 138 371 403 367 385 17 1452 Cuts at: 5402 5488 5500 5846 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 403 385 371 367 346 297 138 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2020 2096 2133 2556 3093 513 Size: 552 955 76 37 423 537 3271 Fragments arranged by size: 3271 955 552 537 423 76 37 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5112 Fragments arranged by size: 5112 683 56 BtsI GCAGTG_nn' Cuts at: 3367 4161 4181 3367 Size: 794 20 5037 Fragments arranged by size: 5037 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5851 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5816 DraI TTT'AAA Cuts at: 3155 3155 Size: 5851 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3074 4858 809 Size: 507 1758 1784 1802 Fragments arranged by size: 1802 1784 1758 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2130 2460 3298 Size: 54 1425 50 275 18 300 330 838 Cuts at: 3298 4187 5816 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 838 330 300 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3298 1537 Size: 275 1486 4090 Fragments arranged by size: 4090 1486 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2528 2669 2673 3781 1353 Size: 1175 141 4 1108 3423 Fragments arranged by size: 3423 1175 1108 141 4 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4438 4812 4958 387 Size: 1064 138 2849 374 146 1280 Fragments arranged by size: 2849 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2794 2794 Size: 5851 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2305 2381 2609 2633 2708 3914 4511 Size: 393 673 76 228 24 75 1206 597 Cuts at: 4511 5298 5566 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1206 787 673 597 393 268 228 76 75 24 EcoO109I rG'GnC_Cy Cuts at: 2212 2699 2212 Size: 487 5364 EcoRI G'AATT_C Cuts at: 3160 3538 3160 Size: 378 5473 FspI TGC'GCA Cuts at: 4357 4357 Size: 5851 HaeII r_GCGC'y Cuts at: 1678 2086 2129 2687 4998 1678 Size: 408 43 558 2311 2531 Fragments arranged by size: 2531 2311 558 408 43 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2278 2310 4492 4524 4566 Size: 32 135 32 706 32 2182 32 42 Cuts at: 4566 4598 1373 Size: 32 2626 Fragments arranged by size: 2626 2182 706 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3195 3441 669 Size: 988 281 1257 246 3079 Fragments arranged by size: 3079 1257 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5851 HpaI GTT'AAC Cuts at: 3441 3441 Size: 5851 KpnI G_GTAC'C Cuts at: 3014 3176 3014 Size: 162 5689 MfeI C'AATT_G Cuts at: 3450 3450 Size: 5851 MluI A'CGCG_T Cuts at: 5727 5727 Size: 5851 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2974 4965 5149 1305 Size: 1669 1991 184 2007 Fragments arranged by size: 2007 1991 1669 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2462 10 Size: 54 1425 343 630 3399 Fragments arranged by size: 3399 1425 630 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3809 4168 4327 518 Size: 648 2643 359 159 2042 Fragments arranged by size: 2643 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2022 2098 2194 2865 3267 3944 5092 5337 Size: 416 76 96 671 402 677 1148 245 Cuts at: 5337 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 671 416 402 245 96 76 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5299 NdeI CA'TA_TG Cuts at: 387 387 Size: 5851 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5851 NotI GC'GGCC_GC Cuts at: 3298 3298 Size: 5851 NspI r_CATG'y Cuts at: 1132 1229 3209 4754 1132 Size: 97 1980 1545 2229 Fragments arranged by size: 2229 1980 1545 97 PasI CC'CwG_GG Cuts at: 1348 2431 1348 Size: 1083 4768 PciI A'CATG_T Cuts at: 4750 4750 Size: 5851 PflMI CCAn_nnn'nTGG Cuts at: 1108 2743 1108 Size: 1635 4216 PfoI T'CCnGG_A Cuts at: 2358 2358 Size: 5851 PmeI GTTT'AAAC Cuts at: 3155 3155 Size: 5851 PmlI CAC'GTG Cuts at: 2398 2398 Size: 5851 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2040 2072 3920 3952 5433 5465 2040 Size: 32 1848 32 1481 32 2426 Fragments arranged by size: 2426 1848 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2212 2699 2212 Size: 487 5364 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5851 PsiI TTA'TAA Cuts at: 3421 3421 Size: 5851 PstI C_TGCA'G Cuts at: 830 2922 3203 830 Size: 2092 281 3478 Fragments arranged by size: 3478 2092 281 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5851 RsrII CG'GwC_CG Cuts at: 2860 2860 Size: 5851 SacI G_AGCT'C Cuts at: 2796 2796 Size: 5851 SacII CC_GC'GG Cuts at: 2023 2099 2023 Size: 76 5775 SalI G'TCGA_C Cuts at: 1936 3193 1936 Size: 1257 4594 SanDI GG'GwC_CC Cuts at: 2212 2699 2212 Size: 487 5364 SapI GCTCTTCn'nnn_ Cuts at: 2528 2669 2528 Size: 141 5710 SbfI CC_TGCA'GG Cuts at: 3203 3203 Size: 5851 ScaI AGT'ACT Cuts at: 4099 5811 4099 Size: 1712 4139 SfcI C'TryA_G Cuts at: 826 1045 2839 2918 3199 4334 5015 5206 826 Size: 219 1794 79 281 1135 681 191 1471 Fragments arranged by size: 1794 1471 1135 681 281 219 191 79 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5851 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5851 SmaI CCC'GGG Cuts at: 1872 2325 2334 2956 3178 1872 Size: 453 9 622 222 4545 Fragments arranged by size: 4545 622 453 222 9 SmlI C'TyrA_G Cuts at: 820 1017 1943 3955 4856 5118 820 Size: 197 926 2012 901 262 1553 Fragments arranged by size: 2012 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3642 493 Size: 3149 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5851 SphI G_CATG'C Cuts at: 1132 3209 1132 Size: 2077 3774 SspI AAT'ATT Cuts at: 5 52 3775 5 Size: 47 3723 2081 Fragments arranged by size: 3723 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5851 StyI C'CwwG_G Cuts at: 513 1065 2474 2703 3290 513 Size: 552 1409 229 587 3074 Fragments arranged by size: 3074 1409 587 552 229 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3102 3106 3878 3895 4048 4233 4678 Size: 1255 1522 4 772 17 153 185 445 Cuts at: 4678 325 Size: 1498 Fragments arranged by size: 1522 1498 1255 772 445 185 153 17 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2943 4097 5809 Size: 274 80 33 51 1443 966 1154 1712 Cuts at: 5809 96 Size: 138 Fragments arranged by size: 1712 1443 1154 966 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5811 5843 5811 Size: 32 5819 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5851 XbaI T'CTAG_A Cuts at: 3187 3187 Size: 5851 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2280 2280 Size: 5851 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5851 XmaI C'CCGG_G Cuts at: 1870 2323 2332 2954 3176 1870 Size: 453 9 622 222 4545 Fragments arranged by size: 4545 622 453 222 9 XmnI GAAnn'nnTTC Cuts at: 2522 3980 2522 Size: 1458 4393 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3659 276 Size: 53 83 186 936 2125 2468 Fragments arranged by size: 2468 2125 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI PvuII SexAI SfiI SfoI SrfI SwaI