Restriction map for
pFN21AB6504
(Linear) (Six-base) MAPSORT of: KIBB6504.seq Check: 2928 from: 1 to: 1146
KIBB6504.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
Acc65I G'GTAC_C
Cuts at: 0 1005 1146
Size: 1005 141
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 703 1146
Size: 703 443
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 307 1073 1146
Size: 307 766 73
Fragments arranged by size:
766 307 73
AvaI C'yCGr_G
Cuts at: 0 318 327 949 1146
Size: 318 9 622 197
Fragments arranged by size:
622 318 197 9
BamHI G'GATC_C
Cuts at: 0 264 402 773 1146
Size: 264 138 371 373
Fragments arranged by size:
373 371 264 138
BanI G'GyrC_C
Cuts at: 0 164 722 1005 1146
Size: 164 558 283 141
Fragments arranged by size:
558 283 164 141
BanII G_rGCy'C
Cuts at: 0 136 791 819 959 1145 1146
Size: 136 655 28 140 186 1
Fragments arranged by size:
655 186 140 136 28 1
BbsI GAAGACnn'nnnn_
Cuts at: 0 980 1146
Size: 980 166
BbvCI CC'TCA_GC
Cuts at: 0 874 1146
Size: 874 272
BlpI GC'TnA_GC
Cuts at: 0 613 1146
Size: 613 533
Bme1580I G_kGCm'C
Cuts at: 0 569 576 1146
Size: 569 7 570
Fragments arranged by size:
570 569 7
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 300 376 604 628 1146
Size: 300 76 228 24 518
Fragments arranged by size:
518 300 228 76 24
Bpu10I CC'TnA_GC
Cuts at: 0 874 1146
Size: 874 272
BsaAI yAC'GTr
Cuts at: 0 393 1146
Size: 393 753
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 998 1028 1146
Size: 998 30 118
Fragments arranged by size:
998 118 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 193 891 1146
Size: 193 698 255
Fragments arranged by size:
698 255 193
BseYI C'CCAG_C
Cuts at: 0 36 189 731 1146
Size: 36 153 542 415
Fragments arranged by size:
542 415 153 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 880 1146
Size: 880 266
BsiHKAI G_wGCw'C
Cuts at: 0 286 791 1016 1146
Size: 286 505 225 130
Fragments arranged by size:
505 286 225 130
BsmI GAATG_Cn'
Cuts at: 0 1109 1146
Size: 1109 37
BsmBI CGTCTCn'nnnn_
Cuts at: 0 280 1146
Size: 280 866
Bsp1286I G_dGCh'C
Cuts at: 0 136 286 569 576 791 819 959 1016
Size: 136 150 283 7 215 28 140 57
Cuts at: 1016 1145 1146
Size: 129 1
Fragments arranged by size:
283 215 150 140 136 129 57 28
7 1
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 373 917 1146
Size: 373 544 229
Fragments arranged by size:
544 373 229
BsrBI CCG'CTC
Cuts at: 0 158 1146
Size: 158 988
BsrDI GCAATG_nn'
Cuts at: 0 453 1146
Size: 453 693
BstEII G'GTnAC_C
Cuts at: 0 642 1146
Size: 642 504
BstYI r'GATC_y
Cuts at: 0 264 402 773 1146
Size: 264 138 371 373
Fragments arranged by size:
373 371 264 138
BtgI C'CryG_G
Cuts at: 0 15 91 128 551 1088 1146
Size: 15 76 37 423 537 58
Fragments arranged by size:
537 423 76 58 37 15
DrdI GACnn_nn'nnGTC
Cuts at: 0 1069 1146
Size: 1069 77
EaeI y'GGCC_r
Cuts at: 0 125 455 1146
Size: 125 330 691
Fragments arranged by size:
691 330 125
EarI CTCTTCn'nnn_
Cuts at: 0 523 664 668 1146
Size: 523 141 4 478
Fragments arranged by size:
523 478 141 4
EcoICRI GAG'CTC
Cuts at: 0 789 1146
Size: 789 357
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 300 376 604 628 703 1146
Size: 300 76 228 24 75 443
Fragments arranged by size:
443 300 228 76 75 24
EcoO109I rG'GnC_Cy
Cuts at: 0 207 694 1146
Size: 207 487 452
Fragments arranged by size:
487 452 207
HaeII r_GCGC'y
Cuts at: 0 81 124 682 1146
Size: 81 43 558 464
Fragments arranged by size:
558 464 81 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 273 305 1146
Size: 273 32 841
Fragments arranged by size:
841 273 32
KpnI G_GTAC'C
Cuts at: 0 1009 1146
Size: 1009 137
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 969 1146
Size: 969 177
MscI TGG'CCA
Cuts at: 0 457 1146
Size: 457 689
MspA1I CmG'CkG
Cuts at: 0 17 93 189 860 1146
Size: 17 76 96 671 286
Fragments arranged by size:
671 286 96 76 17
PasI CC'CwG_GG
Cuts at: 0 426 1146
Size: 426 720
PflMI CCAn_nnn'nTGG
Cuts at: 0 738 1146
Size: 738 408
PfoI T'CCnGG_A
Cuts at: 0 353 1146
Size: 353 793
PmlI CAC'GTG
Cuts at: 0 393 1146
Size: 393 753
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 35 67 1146
Size: 35 32 1079
Fragments arranged by size:
1079 35 32
PpuMI rG'GwC_Cy
Cuts at: 0 207 694 1146
Size: 207 487 452
Fragments arranged by size:
487 452 207
PstI C_TGCA'G
Cuts at: 0 917 1146
Size: 917 229
RsrII CG'GwC_CG
Cuts at: 0 855 1146
Size: 855 291
SacI G_AGCT'C
Cuts at: 0 791 1146
Size: 791 355
SacII CC_GC'GG
Cuts at: 0 18 94 1146
Size: 18 76 1052
Fragments arranged by size:
1052 76 18
SanDI GG'GwC_CC
Cuts at: 0 207 694 1146
Size: 207 487 452
Fragments arranged by size:
487 452 207
SapI GCTCTTCn'nnn_
Cuts at: 0 523 664 1146
Size: 523 141 482
Fragments arranged by size:
523 482 141
SfcI C'TryA_G
Cuts at: 0 834 913 1146
Size: 834 79 233
Fragments arranged by size:
834 233 79
SmaI CCC'GGG
Cuts at: 0 320 329 951 1146
Size: 320 9 622 195
Fragments arranged by size:
622 320 195 9
StyI C'CwwG_G
Cuts at: 0 469 698 1146
Size: 469 229 448
Fragments arranged by size:
469 448 229
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1097 1101 1146
Size: 1097 4 45
Fragments arranged by size:
1097 45 4
TatI w'GTAC_w
Cuts at: 0 938 1146
Size: 938 208
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 275 1146
Size: 275 871
XmaI C'CCGG_G
Cuts at: 0 318 327 949 1146
Size: 318 9 622 197
Fragments arranged by size:
622 318 197 9
XmnI GAAnn'nnTTC
Cuts at: 0 517 1146
Size: 517 629
Enzymes that do cut:
Acc65I AcuI AlfI AvaI BamHI BanI BanII BbsI
BbvCI BlpI Bme1580I BpmI Bpu10I BsaAI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI
BsrDI BstEII BstYI BtgI DrdI EaeI EarI EcoICRI
Eco57MI EcoO109I HaeII Hin4I KpnI MmeI MscI MspA1I
PasI PflMI PfoI PmlI PpiI PpuMI PstI RsrII
SacI SacII SanDI SapI SfcI SmaI StyI TaqII
TatI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BbeI BcgI BciVI BclI
BfrBI BglI BglII BmgBI BmrI BmtI BplI BpuEI
BsaI BsaBI BsaHI BsaWI BsiEI BsiWI BspEI BspHI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII EagI
EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KasI MfeI MluI MslI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PmeI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII SalI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TstI Tth111I XbaI XhoI ZraI