(Circular) (Six-base) MAPSORT of: pFN21AB6483.seq Check: 5209 from: 1 to: 5689 pFN21AB6483 5689 bp With 182 enzymes: SgfI * October 20, 2009 10:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2865 2865 Size: 5689 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3499 278 Size: 53 83 186 936 1963 2468 Fragments arranged by size: 2468 1963 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3032 1937 Size: 1095 4594 Acc65I G'GTAC_C Cuts at: 3010 3010 Size: 5689 AclI AA'CG_TT Cuts at: 1563 3816 4189 1563 Size: 2253 373 3063 Fragments arranged by size: 3063 2253 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3752 5136 5404 1632 Size: 2120 1384 268 1917 Fragments arranged by size: 2120 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5492 AflIII A'CryG_T Cuts at: 1203 2373 2925 4588 5565 1203 Size: 1170 552 1663 977 1327 Fragments arranged by size: 1663 1327 1170 977 552 AgeI A'CCGG_T Cuts at: 4520 4520 Size: 5689 AhdI GACnn_n'nnGTC Cuts at: 4418 4418 Size: 5689 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5689 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5689 AlwNI CAG_nnn'CTG Cuts at: 2839 3432 5004 2839 Size: 593 1572 3524 Fragments arranged by size: 3524 1572 593 ApaI G_GGCC'C Cuts at: 2059 2059 Size: 5689 ApaLI G'TGCA_C Cuts at: 2105 3746 4902 2105 Size: 1641 1156 2892 Fragments arranged by size: 2892 1641 1156 ApoI r'AATT_y Cuts at: 1493 2204 2456 2998 3224 3376 5485 1493 Size: 711 252 542 226 152 2109 1697 Fragments arranged by size: 2109 1697 711 542 252 226 152 AseI AT'TA_AT Cuts at: 160 1032 4243 160 Size: 872 3211 1606 Fragments arranged by size: 3211 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5689 AvaI C'yCGr_G Cuts at: 1870 1943 2502 2556 2585 3014 1870 Size: 73 559 54 29 429 4545 Fragments arranged by size: 4545 559 429 73 54 29 BamHI G'GATC_C Cuts at: 3019 3386 3019 Size: 367 5322 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2781 2973 3010 4465 618 Size: 325 217 627 994 192 37 1455 1842 Fragments arranged by size: 1842 1455 994 627 325 217 192 37 BanII G_rGCy'C Cuts at: 1391 2059 3008 1391 Size: 668 949 4072 Fragments arranged by size: 4072 949 668 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4718 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3880 3914 1903 Size: 34 1943 34 3678 Fragments arranged by size: 3678 1943 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3581 4791 3581 Size: 1210 4479 BclI T'GATC_A Cuts at: 1548 1548 Size: 5689 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2091 4300 136 Size: 107 122 71 631 605 419 2209 1525 Fragments arranged by size: 2209 1525 631 605 419 122 107 71 BglII A'GATC_T Cuts at: 5684 5684 Size: 5689 BlpI GC'TnA_GC Cuts at: 2894 3106 2894 Size: 212 5477 Bme1580I G_kGCm'C Cuts at: 1790 2059 2109 2784 3750 4906 1790 Size: 269 50 675 966 1156 2573 Fragments arranged by size: 2573 1156 966 675 269 50 BmgBI CAC'GTC Cuts at: 1318 2467 1318 Size: 1149 4540 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2818 3420 4378 Size: 416 53 83 399 24 1401 602 958 Cuts at: 4378 442 Size: 1753 Fragments arranged by size: 1753 1401 958 602 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5689 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2148 2148 Size: 5689 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2651 4349 1239 Size: 1412 1698 2579 Fragments arranged by size: 2579 1698 1412 Bpu10I CC'TnA_GC Cuts at: 2123 2123 Size: 5689 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3814 4679 4977 3814 Size: 865 298 4526 Fragments arranged by size: 4526 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2474 4352 882 Size: 617 975 1878 2219 Fragments arranged by size: 2219 1878 975 617 BsaAI yAC'GTr Cuts at: 493 1204 2376 3480 493 Size: 711 1172 1104 2702 Fragments arranged by size: 2702 1172 1104 711 BsaBI GATnn'nnATC Cuts at: 5683 5683 Size: 5689 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2145 3496 3878 4513 Size: 53 83 186 936 612 1351 382 635 Cuts at: 4513 275 Size: 1451 Fragments arranged by size: 1451 1351 936 635 612 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4122 4520 4794 4941 5352 4122 Size: 398 274 147 411 4459 Fragments arranged by size: 4459 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2708 2738 1178 Size: 30 165 30 1305 30 4129 Fragments arranged by size: 4129 1305 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2426 2576 2603 2687 2726 2729 1186 Size: 1240 150 27 84 39 3 4146 Fragments arranged by size: 4146 1240 150 84 39 27 3 BseYI C'CCAG_C Cuts at: 2289 4892 5388 2289 Size: 2603 496 2590 Fragments arranged by size: 2603 2590 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2564 2750 2846 1163 Size: 573 828 186 96 4006 Fragments arranged by size: 4006 828 573 186 96 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3139 3900 4928 1540 Size: 275 186 1138 761 1028 2301 Fragments arranged by size: 2301 1138 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2109 3750 3835 4906 2109 Size: 1641 85 1071 2892 Fragments arranged by size: 2892 1641 1071 85 BsiWI C'GTAC_G Cuts at: 2376 2376 Size: 5689 BsmI GAATG_Cn' Cuts at: 3200 3293 3200 Size: 93 5596 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2059 2109 2784 3008 3750 3835 4906 Size: 399 269 50 675 224 742 85 1071 Cuts at: 4906 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 675 399 269 224 85 50 BspEI T'CCGG_A Cuts at: 5352 5352 Size: 5689 BspHI T'CATG_A Cuts at: 2461 3578 5308 2461 Size: 1117 1730 2842 Fragments arranged by size: 2842 1730 1117 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2865 3044 844 Size: 2021 179 3489 Fragments arranged by size: 3489 2021 179 BsrBI CCG'CTC Cuts at: 1394 1823 2512 3576 1394 Size: 429 689 1064 3507 Fragments arranged by size: 3507 1064 689 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4184 4358 65 Size: 1183 2936 174 1396 Fragments arranged by size: 2936 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2719 4333 4520 1700 Size: 110 909 1614 187 2869 Fragments arranged by size: 2869 1614 909 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5689 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5689 BssSI C'ACGA_G Cuts at: 2864 3749 4761 2864 Size: 885 1012 3792 Fragments arranged by size: 3792 1012 885 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5689 BstBI TT'CG_AA Cuts at: 4493 4493 Size: 5689 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5689 BstYI r'GATC_y Cuts at: 1972 2686 2764 3019 3386 3771 3788 5240 5326 Size: 714 78 255 367 385 17 1452 86 Cuts at: 5326 5338 5684 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 714 385 367 346 255 86 78 17 12 BtgI C'CryG_G Cuts at: 513 1065 2149 513 Size: 552 1084 4053 Fragments arranged by size: 4053 1084 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2665 487 Size: 683 56 1439 3511 Fragments arranged by size: 3511 1439 683 56 BtsI GCAGTG_nn' Cuts at: 3205 3999 4019 3205 Size: 794 20 4875 Fragments arranged by size: 4875 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5689 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5654 DraI TTT'AAA Cuts at: 2993 2993 Size: 5689 DraIII CAC_nnn'GTG Cuts at: 2812 2812 Size: 5689 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4696 809 Size: 507 3380 1802 Fragments arranged by size: 3380 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2092 2196 2301 Size: 54 1425 50 275 18 262 104 105 Cuts at: 2301 2434 2526 2550 3136 4025 5654 8 Size: 133 92 24 586 889 1629 43 Fragments arranged by size: 1629 1425 889 586 275 262 133 105 104 92 54 50 43 24 18 EagI C'GGCC_G Cuts at: 1537 1812 3136 1537 Size: 275 1324 4090 Fragments arranged by size: 4090 1324 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3619 1353 Size: 2266 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2263 4276 4650 4796 387 Size: 1064 138 674 2013 374 146 1280 Fragments arranged by size: 2013 1280 1064 674 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2651 3752 4349 5136 5404 1239 Size: 393 1019 1101 597 787 268 1524 Fragments arranged by size: 1524 1101 1019 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2055 2084 2635 2962 2055 Size: 29 551 327 4782 Fragments arranged by size: 4782 551 327 29 EcoRI G'AATT_C Cuts at: 2204 2998 3376 2204 Size: 794 378 4517 Fragments arranged by size: 4517 794 378 FspI TGC'GCA Cuts at: 2420 4195 2420 Size: 1775 3914 HaeII r_GCGC'y Cuts at: 1678 4836 1678 Size: 3158 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2485 2517 2528 2539 2560 Size: 32 135 32 913 32 11 11 21 Cuts at: 2560 2571 2678 2710 4330 4362 4404 4436 1373 Size: 11 107 32 1620 32 42 32 2626 Fragments arranged by size: 2626 1620 913 135 107 42 32 32 32 32 32 32 21 11 11 11 HincII GTy'rAC Cuts at: 669 1657 1938 3033 3279 669 Size: 988 281 1095 246 3079 Fragments arranged by size: 3079 1095 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5689 HpaI GTT'AAC Cuts at: 3279 3279 Size: 5689 KpnI G_GTAC'C Cuts at: 3014 3014 Size: 5689 MfeI C'AATT_G Cuts at: 3288 3288 Size: 5689 MluI A'CGCG_T Cuts at: 5565 5565 Size: 5689 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4803 4987 1305 Size: 3498 184 2007 Fragments arranged by size: 3498 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2094 2198 2436 2552 10 Size: 54 1425 343 262 104 238 116 3147 Fragments arranged by size: 3147 1425 343 262 238 116 104 54 MslI CAynn'nnrTG Cuts at: 518 1166 2787 2930 2979 3647 4006 4165 518 Size: 648 1621 143 49 668 359 159 2042 Fragments arranged by size: 2042 1621 668 648 359 159 143 49 MspA1I CmG'CkG Cuts at: 1606 2338 3105 3782 4930 5175 1606 Size: 732 767 677 1148 245 2120 Fragments arranged by size: 2120 1148 767 732 677 245 NaeI GCC'GGC Cuts at: 2721 2721 Size: 5689 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5137 NdeI CA'TA_TG Cuts at: 387 387 Size: 5689 NgoMIV G'CCGG_C Cuts at: 2719 2719 Size: 5689 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5689 NotI GC'GGCC_GC Cuts at: 3136 3136 Size: 5689 NspI r_CATG'y Cuts at: 1132 1229 2605 2929 3047 4592 1132 Size: 97 1376 324 118 1545 2229 Fragments arranged by size: 2229 1545 1376 324 118 97 PasI CC'CwG_GG Cuts at: 1348 2059 1348 Size: 711 4978 PciI A'CATG_T Cuts at: 2925 4588 2925 Size: 1663 4026 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 2839 1108 Size: 902 829 3958 Fragments arranged by size: 3958 902 829 PfoI T'CCnGG_A Cuts at: 2751 2760 2751 Size: 9 5680 PmeI GTTT'AAAC Cuts at: 2993 2993 Size: 5689 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3758 3790 5271 5303 3758 Size: 32 1481 32 4144 Fragments arranged by size: 4144 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2635 2962 2635 Size: 327 5362 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5689 PsiI TTA'TAA Cuts at: 3259 3259 Size: 5689 PspOMI G'GGCC_C Cuts at: 2055 2055 Size: 5689 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2147 2179 2147 Size: 32 5657 PstI C_TGCA'G Cuts at: 830 3041 830 Size: 2211 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5689 RsrII CG'GwC_CG Cuts at: 2386 2386 Size: 5689 SalI G'TCGA_C Cuts at: 1936 3031 1936 Size: 1095 4594 SbfI CC_TGCA'GG Cuts at: 3041 3041 Size: 5689 ScaI AGT'ACT Cuts at: 3937 5649 3937 Size: 1712 3977 SfcI C'TryA_G Cuts at: 826 1045 3037 4172 4853 5044 826 Size: 219 1992 1135 681 191 1471 Fragments arranged by size: 1992 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2091 2091 Size: 5689 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5689 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5689 SmaI CCC'GGG Cuts at: 1872 3016 1872 Size: 1144 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2556 3793 4694 4956 820 Size: 197 926 613 1237 901 262 1553 Fragments arranged by size: 1553 1237 926 901 613 262 197 SnaBI TAC'GTA Cuts at: 493 3480 493 Size: 2987 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5689 SphI G_CATG'C Cuts at: 1132 3047 1132 Size: 1915 3774 SspI AAT'ATT Cuts at: 5 52 3613 5 Size: 47 3561 2081 Fragments arranged by size: 3561 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5689 StyI C'CwwG_G Cuts at: 513 1065 2199 3128 513 Size: 552 1134 929 3074 Fragments arranged by size: 3074 1134 929 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2281 2716 2800 3716 3733 3886 4071 Size: 1255 701 435 84 916 17 153 185 Cuts at: 4071 4516 325 Size: 445 1498 Fragments arranged by size: 1498 1255 916 701 445 435 185 153 84 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2676 2691 3935 Size: 274 80 33 51 1443 699 15 1244 Cuts at: 3935 5647 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1244 699 274 138 80 51 33 15 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2178 2210 2708 2740 5649 5681 2178 Size: 32 498 32 2909 32 2186 Fragments arranged by size: 2909 2186 498 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5689 XbaI T'CTAG_A Cuts at: 3025 3025 Size: 5689 XhoI C'TCGA_G Cuts at: 1943 2556 1943 Size: 613 5076 XmaI C'CCGG_G Cuts at: 1870 3014 1870 Size: 1144 4545 XmnI GAAnn'nnTTC Cuts at: 2457 3818 2457 Size: 1361 4328 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3497 276 Size: 53 83 186 936 1963 2468 Fragments arranged by size: 2468 1963 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI RsrII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PvuII SacI SacII SanDI SapI SexAI SfoI SrfI SwaI XcmI