(Circular) (Six-base) MAPSORT of: pFN21AB6478.seq Check: 2970 from: 1 to: 5770 pFN21AB6478 5770 bp With 182 enzymes: SgfI * October 20, 2009 10:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3580 278 Size: 53 83 186 936 2044 2468 Fragments arranged by size: 2468 2044 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3113 1937 Size: 1176 4594 Acc65I G'GTAC_C Cuts at: 3091 3091 Size: 5770 AclI AA'CG_TT Cuts at: 1563 3897 4270 1563 Size: 2334 373 3063 Fragments arranged by size: 3063 2334 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2179 2501 3833 5217 5485 1632 Size: 547 322 1332 1384 268 1917 Fragments arranged by size: 1917 1384 1332 547 322 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5573 AflIII A'CryG_T Cuts at: 1203 2409 4669 5646 1203 Size: 1206 2260 977 1327 Fragments arranged by size: 2260 1327 1206 977 AgeI A'CCGG_T Cuts at: 4601 4601 Size: 5770 AhdI GACnn_n'nnGTC Cuts at: 4499 4499 Size: 5770 AleI CACnn'nnGTG Cuts at: 1166 2157 2462 1166 Size: 991 305 4474 Fragments arranged by size: 4474 991 305 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5770 AlwNI CAG_nnn'CTG Cuts at: 3513 5085 3513 Size: 1572 4198 ApaLI G'TGCA_C Cuts at: 3827 4983 3827 Size: 1156 4614 ApoI r'AATT_y Cuts at: 1493 3079 3305 3457 5566 1493 Size: 1586 226 152 2109 1697 Fragments arranged by size: 2109 1697 1586 226 152 AseI AT'TA_AT Cuts at: 160 1032 4324 160 Size: 872 3292 1606 Fragments arranged by size: 3292 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5770 AvaI C'yCGr_G Cuts at: 1870 1943 3095 1870 Size: 73 1152 4545 Fragments arranged by size: 4545 1152 73 BamHI G'GATC_C Cuts at: 3100 3467 3100 Size: 367 5403 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2159 2468 3091 4546 618 Size: 325 217 627 372 309 623 1455 1842 Fragments arranged by size: 1842 1455 627 623 372 325 309 217 BanII G_rGCy'C Cuts at: 1391 2358 2400 2901 2961 2988 3089 1391 Size: 967 42 501 60 27 101 4072 Fragments arranged by size: 4072 967 501 101 60 42 27 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2127 928 Size: 971 228 4571 Fragments arranged by size: 4571 971 228 BbvCI CC'TCA_GC Cuts at: 2546 2546 Size: 5770 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2732 2766 3961 3995 1903 Size: 34 795 34 1195 34 3678 Fragments arranged by size: 3678 1195 795 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3662 4872 3662 Size: 1210 4560 BclI T'GATC_A Cuts at: 1548 1548 Size: 5770 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2609 2703 4381 Size: 107 122 71 631 605 937 94 1678 Cuts at: 4381 136 Size: 1525 Fragments arranged by size: 1678 1525 937 631 605 122 107 94 71 BglII A'GATC_T Cuts at: 2070 5765 2070 Size: 3695 2075 BlpI GC'TnA_GC Cuts at: 3187 3187 Size: 5770 Bme1580I G_kGCm'C Cuts at: 1790 2471 3831 4987 1790 Size: 681 1360 1156 2573 Fragments arranged by size: 2573 1360 1156 681 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5770 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2575 3501 4459 Size: 416 53 83 399 24 1158 926 958 Cuts at: 4459 442 Size: 1753 Fragments arranged by size: 1753 1158 958 926 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5770 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2114 2327 2837 2870 2915 4430 1239 Size: 875 213 510 33 45 1515 2579 Fragments arranged by size: 2579 1515 875 510 213 45 33 Bpu10I CC'TnA_GC Cuts at: 2368 2511 2546 2368 Size: 143 35 5592 Fragments arranged by size: 5592 143 35 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3895 4760 5058 3895 Size: 865 298 4607 Fragments arranged by size: 4607 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4433 882 Size: 617 2934 2219 Fragments arranged by size: 2934 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3561 493 Size: 711 2357 2702 Fragments arranged by size: 2702 2357 711 BsaBI GATnn'nnATC Cuts at: 5764 5764 Size: 5770 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3577 3959 4594 275 Size: 53 83 186 936 2044 382 635 1451 Fragments arranged by size: 2044 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4203 4601 4875 5022 5433 4203 Size: 398 274 147 411 4540 Fragments arranged by size: 4540 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5545 Fragments arranged by size: 5545 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2102 2240 2312 1186 Size: 916 138 72 4644 Fragments arranged by size: 4644 916 138 72 BseYI C'CCAG_C Cuts at: 2641 2666 2967 3065 4973 5469 2641 Size: 25 301 98 1908 496 2942 Fragments arranged by size: 2942 1908 496 301 98 25 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2321 2485 1163 Size: 573 585 164 4448 Fragments arranged by size: 4448 585 573 164 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2587 2746 3220 3981 5009 1540 Size: 275 186 586 159 474 761 1028 2301 Fragments arranged by size: 2301 1028 761 586 474 275 186 159 BsiHKAI G_wGCw'C Cuts at: 2400 3831 3916 4987 2400 Size: 1431 85 1071 3183 Fragments arranged by size: 3183 1431 1071 85 BsiWI C'GTAC_G Cuts at: 2037 2037 Size: 5770 BsmI GAATG_Cn' Cuts at: 3281 3374 3281 Size: 93 5677 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2358 2400 2471 2901 2961 2988 3089 Size: 399 568 42 71 430 60 27 101 Cuts at: 3089 3831 3916 4987 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 568 430 399 101 85 71 60 42 27 BspEI T'CCGG_A Cuts at: 5433 5433 Size: 5770 BspHI T'CATG_A Cuts at: 2787 2799 3659 5389 2787 Size: 12 860 1730 3168 Fragments arranged by size: 3168 1730 860 12 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2685 2733 3125 844 Size: 1841 48 392 3489 Fragments arranged by size: 3489 1841 392 48 BsrBI CCG'CTC Cuts at: 1394 1823 2098 3007 3657 1394 Size: 429 275 909 650 3507 Fragments arranged by size: 3507 909 650 429 275 BsrDI GCAATG_nn' Cuts at: 65 1248 4265 4439 65 Size: 1183 3017 174 1396 Fragments arranged by size: 3017 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2162 2493 2942 4414 4601 1700 Size: 110 352 331 449 1472 187 2869 Fragments arranged by size: 2869 1472 449 352 331 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5770 BssHII G'CGCG_C Cuts at: 1925 2268 1925 Size: 343 5427 BssSI C'ACGA_G Cuts at: 3830 4842 3830 Size: 1012 4758 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5770 BstBI TT'CG_AA Cuts at: 4574 4574 Size: 5770 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5770 BstXI CCAn_nnnn'nTGG Cuts at: 2641 2641 Size: 5770 BstYI r'GATC_y Cuts at: 1972 2070 2658 2820 3100 3467 3852 3869 5321 Size: 98 588 162 280 367 385 17 1452 Cuts at: 5321 5407 5419 5765 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 588 385 367 346 280 162 98 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2206 2220 2401 2472 2487 2505 2767 Size: 552 1141 14 181 71 15 18 262 Cuts at: 2767 513 Size: 3516 Fragments arranged by size: 3516 1141 552 262 181 71 18 15 14 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2634 487 Size: 683 56 1408 3623 Fragments arranged by size: 3623 1408 683 56 BtsI GCAGTG_nn' Cuts at: 2172 3286 4080 4100 2172 Size: 1114 794 20 3842 Fragments arranged by size: 3842 1114 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5770 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5735 DraI TTT'AAA Cuts at: 3074 3074 Size: 5770 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4777 809 Size: 507 3461 1802 Fragments arranged by size: 3461 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3217 4106 5735 Size: 54 1425 50 275 18 1387 889 1629 Cuts at: 5735 8 Size: 43 Fragments arranged by size: 1629 1425 1387 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3217 1537 Size: 275 1405 4090 Fragments arranged by size: 4090 1405 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3700 1353 Size: 2347 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4357 4731 4877 387 Size: 1064 138 2768 374 146 1280 Fragments arranged by size: 2768 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2398 2398 Size: 5770 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2114 2179 2327 2501 2837 2870 2915 Size: 393 482 65 148 174 336 33 45 Cuts at: 2915 3833 4430 5217 5485 1239 Size: 918 597 787 268 1524 Fragments arranged by size: 1524 918 787 597 482 393 336 268 174 148 65 45 33 EcoO109I rG'GnC_Cy Cuts at: 2201 2590 2201 Size: 389 5381 EcoRI G'AATT_C Cuts at: 3079 3457 3079 Size: 378 5392 FspI TGC'GCA Cuts at: 4276 4276 Size: 5770 HaeII r_GCGC'y Cuts at: 1678 2297 2698 2861 4917 1678 Size: 619 401 163 2056 2531 Fragments arranged by size: 2531 2056 619 401 163 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2445 2477 4411 4443 4485 Size: 32 135 32 873 32 1934 32 42 Cuts at: 4485 4517 1373 Size: 32 2626 Fragments arranged by size: 2626 1934 873 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2722 2779 3114 3360 669 Size: 988 281 784 57 335 246 3079 Fragments arranged by size: 3079 988 784 335 281 246 57 HindIII A'AGCT_T Cuts at: 748 748 Size: 5770 HpaI GTT'AAC Cuts at: 3360 3360 Size: 5770 KpnI G_GTAC'C Cuts at: 3095 3095 Size: 5770 MfeI C'AATT_G Cuts at: 3369 3369 Size: 5770 MluI A'CGCG_T Cuts at: 5646 5646 Size: 5770 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4884 5068 1305 Size: 3579 184 2007 Fragments arranged by size: 3579 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3948 Fragments arranged by size: 3948 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2157 2414 2462 3728 4087 4246 Size: 648 843 148 257 48 1266 359 159 Cuts at: 4246 518 Size: 2042 Fragments arranged by size: 2042 1266 843 648 359 257 159 148 48 MspA1I CmG'CkG Cuts at: 1606 2305 2670 2683 3186 3863 5011 5256 1606 Size: 699 365 13 503 677 1148 245 2120 Fragments arranged by size: 2120 1148 699 677 503 365 245 13 NaeI GCC'GGC Cuts at: 2164 2495 2944 2164 Size: 331 449 4990 Fragments arranged by size: 4990 449 331 NcoI C'CATG_G Cuts at: 513 1065 2220 2472 2767 513 Size: 552 1155 252 295 3516 Fragments arranged by size: 3516 1155 552 295 252 NdeI CA'TA_TG Cuts at: 387 387 Size: 5770 NgoMIV G'CCGG_C Cuts at: 2162 2493 2942 2162 Size: 331 449 4990 Fragments arranged by size: 4990 449 331 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5770 NotI GC'GGCC_GC Cuts at: 3217 3217 Size: 5770 NspI r_CATG'y Cuts at: 1132 1229 2413 3128 4673 1132 Size: 97 1184 715 1545 2229 Fragments arranged by size: 2229 1545 1184 715 97 PasI CC'CwG_GG Cuts at: 1348 2379 1348 Size: 1031 4739 PciI A'CATG_T Cuts at: 2409 4669 2409 Size: 2260 3510 PflMI CCAn_nnn'nTGG Cuts at: 1108 2347 1108 Size: 1239 4531 PfoI T'CCnGG_A Cuts at: 2064 2064 Size: 5770 PmeI GTTT'AAAC Cuts at: 3074 3074 Size: 5770 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3839 3871 5352 5384 3839 Size: 32 1481 32 4225 Fragments arranged by size: 4225 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2201 2590 2201 Size: 389 5381 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5770 PsiI TTA'TAA Cuts at: 3340 3340 Size: 5770 PstI C_TGCA'G Cuts at: 830 2537 2658 2730 3122 830 Size: 1707 121 72 392 3478 Fragments arranged by size: 3478 1707 392 121 72 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5770 PvuII CAG'CTG Cuts at: 2305 2305 Size: 5770 SacI G_AGCT'C Cuts at: 2400 2400 Size: 5770 SalI G'TCGA_C Cuts at: 1936 3112 1936 Size: 1176 4594 SanDI GG'GwC_CC Cuts at: 2201 2201 Size: 5770 SbfI CC_TGCA'GG Cuts at: 2537 3122 2537 Size: 585 5185 ScaI AGT'ACT Cuts at: 4018 5730 4018 Size: 1712 4058 SfcI C'TryA_G Cuts at: 826 1045 2533 2654 2726 3118 4253 4934 5125 Size: 219 1488 121 72 392 1135 681 191 Cuts at: 5125 826 Size: 1471 Fragments arranged by size: 1488 1471 1135 681 392 219 191 121 72 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5770 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5770 SmaI CCC'GGG Cuts at: 1872 3097 1872 Size: 1225 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3874 4775 5037 820 Size: 197 926 1931 901 262 1553 Fragments arranged by size: 1931 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3561 493 Size: 3068 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5770 SphI G_CATG'C Cuts at: 1132 3128 1132 Size: 1996 3774 SspI AAT'ATT Cuts at: 5 52 3694 5 Size: 47 3642 2081 Fragments arranged by size: 3642 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5770 StyI C'CwwG_G Cuts at: 513 1065 2220 2472 2767 2901 3054 3209 513 Size: 552 1155 252 295 134 153 155 3074 Fragments arranged by size: 3074 1155 552 295 252 155 153 134 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3797 3814 3967 4152 4597 325 Size: 1255 2217 17 153 185 445 1498 Fragments arranged by size: 2217 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4016 5728 96 Size: 274 80 33 51 1443 2039 1712 138 Fragments arranged by size: 2039 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5730 5762 5730 Size: 32 5738 Tth111I GACn'n_nGTC Cuts at: 1316 2459 2783 1316 Size: 1143 324 4303 Fragments arranged by size: 4303 1143 324 XbaI T'CTAG_A Cuts at: 3106 3106 Size: 5770 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2386 2890 2386 Size: 504 5266 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5770 XmaI C'CCGG_G Cuts at: 1870 3095 1870 Size: 1225 4545 XmnI GAAnn'nnTTC Cuts at: 3899 3899 Size: 5770 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3578 276 Size: 53 83 186 936 2044 2468 Fragments arranged by size: 2468 2044 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI