(Circular) (Six-base) MAPSORT of: pFN21AB6475.seq Check: 8955 from: 1 to: 5683 pFN21AB6475 5683 bp With 182 enzymes: SgfI * October 20, 2009 10:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2952 2952 Size: 5683 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3493 278 Size: 53 83 186 936 1957 2468 Fragments arranged by size: 2468 1957 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3026 1937 Size: 1089 4594 Acc65I G'GTAC_C Cuts at: 3004 3004 Size: 5683 AclI AA'CG_TT Cuts at: 1563 3810 4183 1563 Size: 2247 373 3063 Fragments arranged by size: 3063 2247 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2858 3746 5130 5398 1632 Size: 1226 888 1384 268 1917 Fragments arranged by size: 1917 1384 1226 888 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5486 AflIII A'CryG_T Cuts at: 1203 2302 4582 5559 1203 Size: 1099 2280 977 1327 Fragments arranged by size: 2280 1327 1099 977 AgeI A'CCGG_T Cuts at: 4514 4514 Size: 5683 AhdI GACnn_n'nnGTC Cuts at: 4412 4412 Size: 5683 AleI CACnn'nnGTG Cuts at: 1166 2600 1166 Size: 1434 4249 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5683 AlwNI CAG_nnn'CTG Cuts at: 2348 2911 3426 4998 2348 Size: 563 515 1572 3033 Fragments arranged by size: 3033 1572 563 515 ApaLI G'TGCA_C Cuts at: 2251 3740 4896 2251 Size: 1489 1156 3038 Fragments arranged by size: 3038 1489 1156 ApoI r'AATT_y Cuts at: 1493 2992 3218 3370 5479 1493 Size: 1499 226 152 2109 1697 Fragments arranged by size: 2109 1697 1499 226 152 AseI AT'TA_AT Cuts at: 160 1032 4237 160 Size: 872 3205 1606 Fragments arranged by size: 3205 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5683 AvaI C'yCGr_G Cuts at: 1870 1943 3008 1870 Size: 73 1065 4545 Fragments arranged by size: 4545 1065 73 BamHI G'GATC_C Cuts at: 3013 3380 3013 Size: 367 5316 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2069 2202 3004 4459 618 Size: 325 217 627 282 133 802 1455 1842 Fragments arranged by size: 1842 1455 802 627 325 282 217 133 BanII G_rGCy'C Cuts at: 1391 3002 1391 Size: 1611 4072 BbeI G_GCGC'C Cuts at: 2073 2073 Size: 5683 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2733 928 Size: 971 834 3878 Fragments arranged by size: 3878 971 834 BbvCI CC'TCA_GC Cuts at: 2198 2198 Size: 5683 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3874 3908 1903 Size: 34 1937 34 3678 Fragments arranged by size: 3678 1937 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3575 4785 3575 Size: 1210 4473 BclI T'GATC_A Cuts at: 1548 2680 1548 Size: 1132 4551 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4294 136 Size: 107 122 71 631 605 2622 1525 Fragments arranged by size: 2622 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5678 5678 Size: 5683 BlpI GC'TnA_GC Cuts at: 3100 3100 Size: 5683 Bme1580I G_kGCm'C Cuts at: 1790 2255 2358 2887 3744 4900 1790 Size: 465 103 529 857 1156 2573 Fragments arranged by size: 2573 1156 857 529 465 103 BmgBI CAC'GTC Cuts at: 1318 2704 1318 Size: 1386 4297 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3414 4372 442 Size: 416 53 83 399 24 1997 958 1753 Fragments arranged by size: 1997 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5683 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2228 2370 2564 2632 4343 1239 Size: 989 142 194 68 1711 2579 Fragments arranged by size: 2579 1711 989 194 142 68 Bpu10I CC'TnA_GC Cuts at: 2198 2198 Size: 5683 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3808 4673 4971 3808 Size: 865 298 4520 Fragments arranged by size: 4520 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2204 4346 882 Size: 617 705 2142 2219 Fragments arranged by size: 2219 2142 705 617 BsaAI yAC'GTr Cuts at: 493 1204 3474 493 Size: 711 2270 2702 Fragments arranged by size: 2702 2270 711 BsaBI GATnn'nnATC Cuts at: 2679 5677 2679 Size: 2998 2685 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2070 3490 3872 4507 Size: 53 83 186 936 537 1420 382 635 Cuts at: 4507 275 Size: 1451 Fragments arranged by size: 1451 1420 936 635 537 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4116 4514 4788 4935 5346 4116 Size: 398 274 147 411 4453 Fragments arranged by size: 4453 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5458 Fragments arranged by size: 5458 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2171 1186 Size: 985 4698 BseYI C'CCAG_C Cuts at: 2857 2951 4886 5382 2857 Size: 94 1935 496 3158 Fragments arranged by size: 3158 1935 496 94 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2165 1163 Size: 573 429 4681 Fragments arranged by size: 4681 573 429 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3133 3894 4922 1540 Size: 275 186 1132 761 1028 2301 Fragments arranged by size: 2301 1132 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2223 2255 2566 3744 3829 4900 2223 Size: 32 311 1178 85 1071 3006 Fragments arranged by size: 3006 1178 1071 311 85 32 BsmI GAATG_Cn' Cuts at: 2051 3194 3287 2051 Size: 1143 93 4447 Fragments arranged by size: 4447 1143 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2223 2255 2358 2566 2887 3002 3744 Size: 399 433 32 103 208 321 115 742 Cuts at: 3744 3829 4900 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 433 399 321 208 115 103 85 32 BspEI T'CCGG_A Cuts at: 5346 5346 Size: 5683 BspHI T'CATG_A Cuts at: 3572 5302 3572 Size: 1730 3953 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2952 3038 844 Size: 2108 86 3489 Fragments arranged by size: 3489 2108 86 BsrBI CCG'CTC Cuts at: 1394 1823 2170 3570 1394 Size: 429 347 1400 3507 Fragments arranged by size: 3507 1400 429 347 BsrDI GCAATG_nn' Cuts at: 65 1248 4178 4352 65 Size: 1183 2930 174 1396 Fragments arranged by size: 2930 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2150 4327 4514 1700 Size: 110 340 2177 187 2869 Fragments arranged by size: 2869 2177 340 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5683 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5683 BssSI C'ACGA_G Cuts at: 3743 4755 3743 Size: 1012 4671 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5683 BstBI TT'CG_AA Cuts at: 4487 4487 Size: 5683 BstEII G'GTnAC_C Cuts at: 1184 2461 1184 Size: 1277 4406 BstXI CCAn_nnnn'nTGG Cuts at: 2329 2602 2329 Size: 273 5410 BstYI r'GATC_y Cuts at: 1972 2605 3013 3380 3765 3782 5234 5320 5332 Size: 633 408 367 385 17 1452 86 12 Cuts at: 5332 5678 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 633 408 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2601 513 Size: 552 939 597 3595 Fragments arranged by size: 3595 939 597 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2417 487 Size: 683 56 1191 3753 Fragments arranged by size: 3753 1191 683 56 BtsI GCAGTG_nn' Cuts at: 3199 3993 4013 3199 Size: 794 20 4869 Fragments arranged by size: 4869 794 20 ClaI AT'CG_AT Cuts at: 1554 2811 1554 Size: 1257 4426 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5648 DraI TTT'AAA Cuts at: 2987 2987 Size: 5683 DraIII CAC_nnn'GTG Cuts at: 2553 2553 Size: 5683 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4690 809 Size: 507 3374 1802 Fragments arranged by size: 3374 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3130 4019 5648 Size: 54 1425 50 275 18 1300 889 1629 Cuts at: 5648 8 Size: 43 Fragments arranged by size: 1629 1425 1300 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3130 1537 Size: 275 1318 4090 Fragments arranged by size: 4090 1318 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3613 1353 Size: 2260 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2069 4270 4644 4790 387 Size: 1064 138 480 2201 374 146 1280 Fragments arranged by size: 2201 1280 1064 480 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2228 2370 2564 2632 2858 3746 4343 Size: 393 596 142 194 68 226 888 597 Cuts at: 4343 5130 5398 1239 Size: 787 268 1524 Fragments arranged by size: 1524 888 787 597 596 393 268 226 194 142 68 EcoO109I rG'GnC_Cy Cuts at: 2908 2908 Size: 5683 EcoRI G'AATT_C Cuts at: 2992 3370 2992 Size: 378 5305 EcoRV GAT'ATC Cuts at: 2809 2809 Size: 5683 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2849 2849 Size: 5683 FspI TGC'GCA Cuts at: 4189 4189 Size: 5683 HaeII r_GCGC'y Cuts at: 1678 2073 4830 1678 Size: 395 2757 2531 Fragments arranged by size: 2757 2531 395 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4324 4356 4398 4430 1373 Size: 32 135 32 2752 32 42 32 2626 Fragments arranged by size: 2752 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3027 3273 669 Size: 988 281 1089 246 3079 Fragments arranged by size: 3079 1089 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5683 HpaI GTT'AAC Cuts at: 3273 3273 Size: 5683 KasI G'GCGC_C Cuts at: 2069 2069 Size: 5683 KpnI G_GTAC'C Cuts at: 3008 3008 Size: 5683 MfeI C'AATT_G Cuts at: 2787 3282 2787 Size: 495 5188 MluI A'CGCG_T Cuts at: 5559 5559 Size: 5683 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4797 4981 1305 Size: 3492 184 2007 Fragments arranged by size: 3492 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3861 Fragments arranged by size: 3861 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2459 2600 3641 4000 4159 518 Size: 648 1293 141 1041 359 159 2042 Fragments arranged by size: 2042 1293 1041 648 359 159 141 MspA1I CmG'CkG Cuts at: 1606 2472 3099 3776 4924 5169 1606 Size: 866 627 677 1148 245 2120 Fragments arranged by size: 2120 1148 866 677 627 245 NarI GG'CG_CC Cuts at: 2070 2070 Size: 5683 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4192 Fragments arranged by size: 4192 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5683 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5683 NotI GC'GGCC_GC Cuts at: 3130 3130 Size: 5683 NruI TCG'CGA Cuts at: 2436 2436 Size: 5683 NspI r_CATG'y Cuts at: 1132 1229 2167 2306 3041 4586 1132 Size: 97 938 139 735 1545 2229 Fragments arranged by size: 2229 1545 938 735 139 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5683 PciI A'CATG_T Cuts at: 2302 4582 2302 Size: 2280 3403 PflMI CCAn_nnn'nTGG Cuts at: 1108 2348 2911 1108 Size: 1240 563 3880 Fragments arranged by size: 3880 1240 563 PfoI T'CCnGG_A Cuts at: 2904 2904 Size: 5683 PmeI GTTT'AAAC Cuts at: 2987 2987 Size: 5683 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3752 3784 5265 5297 3752 Size: 32 1481 32 4138 Fragments arranged by size: 4138 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2908 2908 Size: 5683 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5683 PsiI TTA'TAA Cuts at: 3253 3253 Size: 5683 PstI C_TGCA'G Cuts at: 830 3035 830 Size: 2205 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5683 SalI G'TCGA_C Cuts at: 1936 3025 1936 Size: 1089 4594 SbfI CC_TGCA'GG Cuts at: 3035 3035 Size: 5683 ScaI AGT'ACT Cuts at: 3931 5643 3931 Size: 1712 3971 SfcI C'TryA_G Cuts at: 826 1045 2512 2878 3031 4166 4847 5038 826 Size: 219 1467 366 153 1135 681 191 1471 Fragments arranged by size: 1471 1467 1135 681 366 219 191 153 SfoI GGC'GCC Cuts at: 2071 2071 Size: 5683 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5683 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5683 SmaI CCC'GGG Cuts at: 1872 3010 1872 Size: 1138 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3787 4688 4950 820 Size: 197 926 1844 901 262 1553 Fragments arranged by size: 1844 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3474 493 Size: 2981 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5683 SphI G_CATG'C Cuts at: 1132 2167 3041 1132 Size: 1035 874 3774 Fragments arranged by size: 3774 1035 874 SspI AAT'ATT Cuts at: 5 52 3607 5 Size: 47 3555 2081 Fragments arranged by size: 3555 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5683 StyI C'CwwG_G Cuts at: 513 1065 2004 3122 513 Size: 552 939 1118 3074 Fragments arranged by size: 3074 1118 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2910 3710 3727 3880 4065 4510 325 Size: 1255 1330 800 17 153 185 445 1498 Fragments arranged by size: 1498 1330 1255 800 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3929 5641 96 Size: 274 80 33 51 1443 1952 1712 138 Fragments arranged by size: 1952 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2006 2038 5643 5675 2006 Size: 32 3605 32 2014 Fragments arranged by size: 3605 2014 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5683 XbaI T'CTAG_A Cuts at: 3019 3019 Size: 5683 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2185 2185 Size: 5683 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5683 XmaI C'CCGG_G Cuts at: 1870 3008 1870 Size: 1138 4545 XmnI GAAnn'nnTTC Cuts at: 2084 2640 3812 2084 Size: 556 1172 3955 Fragments arranged by size: 3955 1172 556 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3491 276 Size: 53 83 186 936 1957 2468 Fragments arranged by size: 2468 1957 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI FseI FspAI NaeI NgoMIV NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI