Restriction map for
pFN21AB6448
(Linear) (Six-base) MAPSORT of: KIBB6448.seq Check: 357 from: 1 to: 1047
KIBB6448.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 597 1047
Size: 597 450
AleI CACnn'nnGTG
Cuts at: 0 704 1047
Size: 704 343
ApaI G_GGCC'C
Cuts at: 0 1013 1047
Size: 1013 34
ApoI r'AATT_y
Cuts at: 0 370 1047
Size: 370 677
AseI AT'TA_AT
Cuts at: 0 509 1047
Size: 509 538
AvaI C'yCGr_G
Cuts at: 0 47 149 1047
Size: 47 102 898
Fragments arranged by size:
898 102 47
BanII G_rGCy'C
Cuts at: 0 47 66 900 1013 1047
Size: 47 19 834 113 34
Fragments arranged by size:
834 113 47 34 19
BciVI GTATCCnnnnn_n'
Cuts at: 0 221 1047
Size: 221 826
BclI T'GATC_A
Cuts at: 0 1028 1047
Size: 1028 19
BfrBI ATG'CAT
Cuts at: 0 647 1047
Size: 647 400
Bme1580I G_kGCm'C
Cuts at: 0 1013 1047
Size: 1013 34
BmrI ACTGGGnnnn_n'
Cuts at: 0 84 1047
Size: 84 963
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 80 1047
Size: 59 21 967
Fragments arranged by size:
967 59 21
BsaBI GATnn'nnATC
Cuts at: 0 868 1047
Size: 868 179
BsaWI w'CCGG_w
Cuts at: 0 660 1047
Size: 660 387
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 67 172 190 428 1047
Size: 67 105 18 238 619
Fragments arranged by size:
619 238 105 67 18
BseYI C'CCAG_C
Cuts at: 0 533 1047
Size: 533 514
Bsp1286I G_dGCh'C
Cuts at: 0 47 66 900 1013 1047
Size: 47 19 834 113 34
Fragments arranged by size:
834 113 47 34 19
BspEI T'CCGG_A
Cuts at: 0 660 1047
Size: 660 387
BspHI T'CATG_A
Cuts at: 0 375 1047
Size: 375 672
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 248 563 597 1047
Size: 248 315 34 450
Fragments arranged by size:
450 315 248 34
BsrDI GCAATG_nn'
Cuts at: 0 264 1047
Size: 264 783
BsrGI T'GTAC_A
Cuts at: 0 275 1047
Size: 275 772
BstXI CCAn_nnnn'nTGG
Cuts at: 0 827 1047
Size: 827 220
EaeI y'GGCC_r
Cuts at: 0 323 947 1047
Size: 323 624 100
Fragments arranged by size:
624 323 100
EarI CTCTTCn'nnn_
Cuts at: 0 416 1047
Size: 416 631
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 31 1047
Size: 31 1016
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 80 1047
Size: 59 21 967
Fragments arranged by size:
967 59 21
EcoO109I rG'GnC_Cy
Cuts at: 0 228 1009 1010 1047
Size: 228 781 1 37
Fragments arranged by size:
781 228 37 1
FspI TGC'GCA
Cuts at: 0 97 1047
Size: 97 950
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 417 449 576 608 1047
Size: 417 32 127 32 439
Fragments arranged by size:
439 417 127 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 377 1047
Size: 377 670
MscI TGG'CCA
Cuts at: 0 325 949 1047
Size: 325 624 98
Fragments arranged by size:
624 325 98
MslI CAynn'nnrTG
Cuts at: 0 568 599 704 820 874 1047
Size: 568 31 105 116 54 173
Fragments arranged by size:
568 173 116 105 54 31
MspA1I CmG'CkG
Cuts at: 0 13 1047
Size: 13 1034
NsiI A_TGCA'T
Cuts at: 0 649 1047
Size: 649 398
PflMI CCAn_nnn'nTGG
Cuts at: 0 771 1047
Size: 771 276
PpuMI rG'GwC_Cy
Cuts at: 0 228 1047
Size: 228 819
PspOMI G'GGCC_C
Cuts at: 0 1009 1047
Size: 1009 38
PstI C_TGCA'G
Cuts at: 0 398 440 1047
Size: 398 42 607
Fragments arranged by size:
607 398 42
SfcI C'TryA_G
Cuts at: 0 394 436 1047
Size: 394 42 611
Fragments arranged by size:
611 394 42
SmaI CCC'GGG
Cuts at: 0 49 151 1047
Size: 49 102 896
Fragments arranged by size:
896 102 49
SspI AAT'ATT
Cuts at: 0 252 1047
Size: 252 795
StuI AGG'CCT
Cuts at: 0 985 1047
Size: 985 62
StyI C'CwwG_G
Cuts at: 0 986 1047
Size: 986 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 208 304 716 1047
Size: 208 96 412 331
Fragments arranged by size:
412 331 208 96
TatI w'GTAC_w
Cuts at: 0 275 302 824 1047
Size: 275 27 522 223
Fragments arranged by size:
522 275 223 27
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 772 827 1047
Size: 772 55 220
Fragments arranged by size:
772 220 55
XmaI C'CCGG_G
Cuts at: 0 47 149 1047
Size: 47 102 898
Fragments arranged by size:
898 102 47
Enzymes that do cut:
AarI AleI ApaI ApoI AseI AvaI BanII BciVI
BclI BfrBI Bme1580I BmrI BpmI BsaBI BsaWI BseRI
BseYI Bsp1286I BspEI BspHI BspMI BsrDI BsrGI BstXI
EaeI EarI EciI Eco57MI EcoO109I FspI Hin4I MmeI
MscI MslI MspA1I NsiI PflMI PpuMI PspOMI PstI
SfcI SmaI SspI StuI StyI TaqII TatI XcmI
XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AloI AlwNI ApaLI AscI AsiSI
AvrII BaeI BamHI BanI BbeI BbsI BbvCI BcgI
BglI BglII BlpI BmgBI BmtI BplI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaXI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI BsrBI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EagI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspI PacI PasI PciI
PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SwaI TstI Tth111I XbaI
XhoI XmnI ZraI