(Circular) (Six-base) MAPSORT of: pFN21AB6427.seq Check: 6907 from: 1 to: 5623 pFN21AB6427 5623 bp With 182 enzymes: SgfI * October 20, 2009 10:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2860 2860 Size: 5623 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3433 278 Size: 53 83 186 936 1897 2468 Fragments arranged by size: 2468 1897 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2966 1937 Size: 1029 4594 Acc65I G'GTAC_C Cuts at: 2944 2944 Size: 5623 AclI AA'CG_TT Cuts at: 1563 3750 4123 1563 Size: 2187 373 3063 Fragments arranged by size: 3063 2187 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2164 2297 2459 3686 5070 5338 1632 Size: 532 133 162 1227 1384 268 1917 Fragments arranged by size: 1917 1384 1227 532 268 162 133 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5426 AflIII A'CryG_T Cuts at: 1203 4522 5499 1203 Size: 3319 977 1327 Fragments arranged by size: 3319 1327 977 AgeI A'CCGG_T Cuts at: 4454 4454 Size: 5623 AhdI GACnn_n'nnGTC Cuts at: 4352 4352 Size: 5623 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5623 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5623 AlwNI CAG_nnn'CTG Cuts at: 2035 2149 2404 3366 4938 2035 Size: 114 255 962 1572 2720 Fragments arranged by size: 2720 1572 962 255 114 ApaLI G'TGCA_C Cuts at: 2247 3680 4836 2247 Size: 1433 1156 3034 Fragments arranged by size: 3034 1433 1156 ApoI r'AATT_y Cuts at: 1493 2654 2932 3158 3310 5419 1493 Size: 1161 278 226 152 2109 1697 Fragments arranged by size: 2109 1697 1161 278 226 152 AseI AT'TA_AT Cuts at: 160 1032 4177 160 Size: 872 3145 1606 Fragments arranged by size: 3145 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5623 AvaI C'yCGr_G Cuts at: 1870 1943 2302 2948 1870 Size: 73 359 646 4545 Fragments arranged by size: 4545 646 359 73 AvrII C'CTAG_G Cuts at: 2371 2371 Size: 5623 BamHI G'GATC_C Cuts at: 2953 3320 2953 Size: 367 5256 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2944 4399 618 Size: 325 217 627 1157 1455 1842 Fragments arranged by size: 1842 1455 1157 627 325 217 BanII G_rGCy'C Cuts at: 1391 2042 2487 2942 1391 Size: 651 445 455 4072 Fragments arranged by size: 4072 651 455 445 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2823 928 Size: 971 924 3728 Fragments arranged by size: 3728 971 924 BbvCI CC'TCA_GC Cuts at: 2586 2586 Size: 5623 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3814 3848 1903 Size: 34 1877 34 3678 Fragments arranged by size: 3678 1877 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3515 4725 3515 Size: 1210 4413 BclI T'GATC_A Cuts at: 1548 1548 Size: 5623 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4234 136 Size: 107 122 71 631 605 2562 1525 Fragments arranged by size: 2562 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5618 5618 Size: 5623 BlpI GC'TnA_GC Cuts at: 2036 3040 2036 Size: 1004 4619 Bme1580I G_kGCm'C Cuts at: 1790 2251 2799 3684 4840 1790 Size: 461 548 885 1156 2573 Fragments arranged by size: 2573 1156 885 548 461 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5623 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2244 2539 2773 Size: 416 53 83 399 24 827 295 234 Cuts at: 2773 3354 4312 442 Size: 581 958 1753 Fragments arranged by size: 1753 958 827 581 416 399 295 234 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5623 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2405 2630 4283 1239 Size: 1166 225 1653 2579 Fragments arranged by size: 2579 1653 1166 225 Bpu10I CC'TnA_GC Cuts at: 2195 2586 2195 Size: 391 5232 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3748 4613 4911 3748 Size: 865 298 4460 Fragments arranged by size: 4460 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2702 4286 882 Size: 617 1203 1584 2219 Fragments arranged by size: 2219 1584 1203 617 BsaAI yAC'GTr Cuts at: 493 1204 3414 493 Size: 711 2210 2702 Fragments arranged by size: 2702 2210 711 BsaBI GATnn'nnATC Cuts at: 5617 5617 Size: 5623 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3430 3812 4447 275 Size: 53 83 186 936 1897 382 635 1451 Fragments arranged by size: 1897 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4056 4454 4728 4875 5286 4056 Size: 398 274 147 411 4393 Fragments arranged by size: 4393 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2334 2364 1178 Size: 30 165 30 931 30 4437 Fragments arranged by size: 4437 931 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2290 2477 2660 1186 Size: 1104 187 183 4149 Fragments arranged by size: 4149 1104 187 183 BseYI C'CCAG_C Cuts at: 2142 2803 2880 4826 5322 2142 Size: 661 77 1946 496 2443 Fragments arranged by size: 2443 1946 661 496 77 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5050 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3073 3834 4862 1540 Size: 275 186 1072 761 1028 2301 Fragments arranged by size: 2301 1072 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2042 2251 2309 2487 2541 3684 3769 4840 2042 Size: 209 58 178 54 1143 85 1071 2825 Fragments arranged by size: 2825 1143 1071 209 178 85 58 54 BsmI GAATG_Cn' Cuts at: 2908 3134 3227 2908 Size: 226 93 5304 Fragments arranged by size: 5304 226 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2165 2165 Size: 5623 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2042 2251 2309 2487 2541 2799 2942 Size: 399 252 209 58 178 54 258 143 Cuts at: 2942 3684 3769 4840 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 258 252 209 178 143 85 58 54 BspEI T'CCGG_A Cuts at: 5286 5286 Size: 5623 BspHI T'CATG_A Cuts at: 3512 5242 3512 Size: 1730 3893 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2466 2522 2860 2978 844 Size: 1622 56 338 118 3489 Fragments arranged by size: 3489 1622 338 118 56 BsrBI CCG'CTC Cuts at: 1394 1823 3510 1394 Size: 429 1687 3507 Fragments arranged by size: 3507 1687 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4118 4292 65 Size: 1183 2870 174 1396 Fragments arranged by size: 2870 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2909 4267 4454 1700 Size: 110 1099 1358 187 2869 Fragments arranged by size: 2869 1358 1099 187 110 BsrGI T'GTAC_A Cuts at: 96 2662 96 Size: 2566 3057 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5623 BssSI C'ACGA_G Cuts at: 2798 3683 4695 2798 Size: 885 1012 3726 Fragments arranged by size: 3726 1012 885 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5623 BstBI TT'CG_AA Cuts at: 4427 4427 Size: 5623 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5623 BstXI CCAn_nnnn'nTGG Cuts at: 2787 2840 2787 Size: 53 5570 BstYI r'GATC_y Cuts at: 1972 2953 3320 3705 3722 5174 5260 5272 5618 Size: 981 367 385 17 1452 86 12 346 Cuts at: 5618 1972 Size: 1977 Fragments arranged by size: 1977 1452 981 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2169 2338 2451 513 Size: 552 939 165 169 113 3685 Fragments arranged by size: 3685 939 552 169 165 113 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4884 Fragments arranged by size: 4884 683 56 BtsI GCAGTG_nn' Cuts at: 3139 3933 3953 3139 Size: 794 20 4809 Fragments arranged by size: 4809 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5623 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5588 DraI TTT'AAA Cuts at: 2927 2927 Size: 5623 DraIII CAC_nnn'GTG Cuts at: 2626 2626 Size: 5623 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4630 809 Size: 507 3314 1802 Fragments arranged by size: 3314 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2842 2919 Size: 54 1425 50 275 18 177 835 77 Cuts at: 2919 3070 3959 5588 8 Size: 151 889 1629 43 Fragments arranged by size: 1629 1425 889 835 275 177 151 77 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3070 1537 Size: 275 1258 4090 Fragments arranged by size: 4090 1258 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2435 3553 1353 Size: 1082 1118 3423 Fragments arranged by size: 3423 1118 1082 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2606 2914 4210 4584 4730 387 Size: 1064 138 1017 308 1296 374 146 1280 Fragments arranged by size: 1296 1280 1064 1017 374 308 146 138 EcoICRI GAG'CTC Cuts at: 2040 2485 2040 Size: 445 5178 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2164 2297 2405 2459 2630 3686 4283 Size: 393 532 133 108 54 171 1056 597 Cuts at: 4283 5070 5338 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1056 787 597 532 393 268 171 133 108 54 EcoNI CCTnn'n_nnAGG Cuts at: 2315 2315 Size: 5623 EcoO109I rG'GnC_Cy Cuts at: 2238 2401 2511 2238 Size: 163 110 5350 Fragments arranged by size: 5350 163 110 EcoRI G'AATT_C Cuts at: 2932 3310 2932 Size: 378 5245 EcoRV GAT'ATC Cuts at: 2026 2026 Size: 5623 FspI TGC'GCA Cuts at: 4129 4129 Size: 5623 HaeII r_GCGC'y Cuts at: 1678 4770 1678 Size: 3092 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2098 2130 4264 4296 4338 Size: 32 135 32 526 32 2134 32 42 Cuts at: 4338 4370 1373 Size: 32 2626 Fragments arranged by size: 2626 2134 526 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2527 2967 3213 669 Size: 988 281 589 440 246 3079 Fragments arranged by size: 3079 988 589 440 281 246 HindIII A'AGCT_T Cuts at: 748 2283 748 Size: 1535 4088 HpaI GTT'AAC Cuts at: 3213 3213 Size: 5623 KpnI G_GTAC'C Cuts at: 2948 2948 Size: 5623 MfeI C'AATT_G Cuts at: 3222 3222 Size: 5623 MluI A'CGCG_T Cuts at: 5499 5499 Size: 5623 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4737 4921 1305 Size: 3432 184 2007 Fragments arranged by size: 3432 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 3624 Fragments arranged by size: 3624 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2070 2228 3581 3940 4099 518 Size: 648 904 158 1353 359 159 2042 Fragments arranged by size: 2042 1353 904 648 359 159 158 MspA1I CmG'CkG Cuts at: 1606 2035 2254 2550 2608 2847 2880 3039 3716 Size: 429 219 296 58 239 33 159 677 Cuts at: 3716 4864 5109 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 429 296 245 239 219 159 58 33 NcoI C'CATG_G Cuts at: 513 1065 2004 2169 513 Size: 552 939 165 3967 Fragments arranged by size: 3967 939 552 165 NdeI CA'TA_TG Cuts at: 387 387 Size: 5623 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5623 NotI GC'GGCC_GC Cuts at: 3070 3070 Size: 5623 NspI r_CATG'y Cuts at: 1132 1229 2981 4526 1132 Size: 97 1752 1545 2229 Fragments arranged by size: 2229 1752 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5623 PciI A'CATG_T Cuts at: 4522 4522 Size: 5623 PflMI CCAn_nnn'nTGG Cuts at: 1108 2149 1108 Size: 1041 4582 PfoI T'CCnGG_A Cuts at: 2570 2570 Size: 5623 PmeI GTTT'AAAC Cuts at: 2927 2927 Size: 5623 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3692 3724 5205 5237 3692 Size: 32 1481 32 4078 Fragments arranged by size: 4078 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2238 2401 2511 2238 Size: 163 110 5350 Fragments arranged by size: 5350 163 110 PshAI GACnn'nnGTC Cuts at: 1613 2318 1613 Size: 705 4918 PsiI TTA'TAA Cuts at: 3193 3193 Size: 5623 PspXI vC'TCGA_Gb Cuts at: 2302 2302 Size: 5623 PstI C_TGCA'G Cuts at: 830 2871 2975 830 Size: 2041 104 3478 Fragments arranged by size: 3478 2041 104 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5623 PvuII CAG'CTG Cuts at: 2035 2254 2550 2608 2035 Size: 219 296 58 5050 Fragments arranged by size: 5050 296 219 58 SacI G_AGCT'C Cuts at: 2042 2487 2042 Size: 445 5178 SalI G'TCGA_C Cuts at: 1936 2965 1936 Size: 1029 4594 SanDI GG'GwC_CC Cuts at: 2238 2238 Size: 5623 SapI GCTCTTCn'nnn_ Cuts at: 2435 2435 Size: 5623 SbfI CC_TGCA'GG Cuts at: 2871 2975 2871 Size: 104 5519 ScaI AGT'ACT Cuts at: 2600 3871 5583 2600 Size: 1271 1712 2640 Fragments arranged by size: 2640 1712 1271 SexAI A'CCwGG_T Cuts at: 2622 2622 Size: 5623 SfcI C'TryA_G Cuts at: 826 1045 2743 2867 2971 4106 4787 4978 826 Size: 219 1698 124 104 1135 681 191 1471 Fragments arranged by size: 1698 1471 1135 681 219 191 124 104 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5623 SgrAI Cr'CCGG_yG Cuts at: 1700 2909 1700 Size: 1209 4414 SmaI CCC'GGG Cuts at: 1872 2950 1872 Size: 1078 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2302 3727 4628 4890 820 Size: 197 926 359 1425 901 262 1553 Fragments arranged by size: 1553 1425 926 901 359 262 197 SnaBI TAC'GTA Cuts at: 493 3414 493 Size: 2921 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5623 SphI G_CATG'C Cuts at: 1132 2981 1132 Size: 1849 3774 SspI AAT'ATT Cuts at: 5 52 3547 5 Size: 47 3495 2081 Fragments arranged by size: 3495 2081 47 StuI AGG'CCT Cuts at: 1517 2579 1517 Size: 1062 4561 StyI C'CwwG_G Cuts at: 513 1065 2004 2010 2169 2371 2542 3062 513 Size: 552 939 6 159 202 171 520 3074 Fragments arranged by size: 3074 939 552 520 202 171 159 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2342 2351 2521 2716 2795 2872 3650 Size: 1255 762 9 170 195 79 77 778 Cuts at: 3650 3667 3820 4005 4450 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1255 778 762 445 195 185 170 153 79 77 17 9 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2598 2662 3869 Size: 274 80 33 51 1443 621 64 1207 Cuts at: 3869 5581 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1207 621 274 138 80 64 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2334 2366 5583 5615 2334 Size: 32 3217 32 2342 Fragments arranged by size: 3217 2342 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2236 1316 Size: 920 4703 XbaI T'CTAG_A Cuts at: 2959 2959 Size: 5623 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2914 2914 Size: 5623 XhoI C'TCGA_G Cuts at: 1943 2302 1943 Size: 359 5264 XmaI C'CCGG_G Cuts at: 1870 2948 1870 Size: 1078 4545 XmnI GAAnn'nnTTC Cuts at: 2444 2678 3752 2444 Size: 234 1074 4315 Fragments arranged by size: 4315 1074 234 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3431 276 Size: 53 83 186 936 1897 2468 Fragments arranged by size: 2468 1897 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI RsrII SacII SfiI SfoI SrfI SwaI