(Circular) (Six-base) MAPSORT of: pFN21AE0102.seq Check: 1209 from: 1 to: 5782 pFN21AE0102 5782 bp With 182 enzymes: SgfI * August 27, 2010 15:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3592 278 Size: 53 83 186 936 2056 2468 Fragments arranged by size: 2468 2056 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3125 1937 Size: 1188 4594 Acc65I G'GTAC_C Cuts at: 3103 3103 Size: 5782 AclI AA'CG_TT Cuts at: 1563 3909 4282 1563 Size: 2346 373 3063 Fragments arranged by size: 3063 2346 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2468 3845 5229 5497 1632 Size: 836 1377 1384 268 1917 Fragments arranged by size: 1917 1384 1377 836 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5585 AflIII A'CryG_T Cuts at: 1203 4681 5658 1203 Size: 3478 977 1327 Fragments arranged by size: 3478 1327 977 AgeI A'CCGG_T Cuts at: 4613 4613 Size: 5782 AhdI GACnn_n'nnGTC Cuts at: 4511 4511 Size: 5782 AleI CACnn'nnGTG Cuts at: 1166 2396 1166 Size: 1230 4552 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5782 AlwNI CAG_nnn'CTG Cuts at: 3055 3525 5097 3055 Size: 470 1572 3740 Fragments arranged by size: 3740 1572 470 ApaI G_GGCC'C Cuts at: 2666 2666 Size: 5782 ApaLI G'TGCA_C Cuts at: 3839 4995 3839 Size: 1156 4626 ApoI r'AATT_y Cuts at: 1493 3091 3317 3469 5578 1493 Size: 1598 226 152 2109 1697 Fragments arranged by size: 2109 1697 1598 226 152 AseI AT'TA_AT Cuts at: 160 1032 4336 160 Size: 872 3304 1606 Fragments arranged by size: 3304 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5782 AvaI C'yCGr_G Cuts at: 1870 1943 3107 1870 Size: 73 1164 4545 Fragments arranged by size: 4545 1164 73 BamHI G'GATC_C Cuts at: 2931 3112 3479 2931 Size: 181 367 5234 Fragments arranged by size: 5234 367 181 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2060 2276 2321 2360 2525 Size: 325 217 627 273 216 45 39 165 Cuts at: 2525 2603 2948 3103 4558 618 Size: 78 345 155 1455 1842 Fragments arranged by size: 1842 1455 627 345 325 273 217 216 165 155 78 45 39 BanII G_rGCy'C Cuts at: 1391 2473 2666 3101 1391 Size: 1082 193 435 4072 Fragments arranged by size: 4072 1082 435 193 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4811 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3973 4007 1903 Size: 34 2036 34 3678 Fragments arranged by size: 3678 2036 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3674 4884 3674 Size: 1210 4572 BclI T'GATC_A Cuts at: 1548 2538 3015 1548 Size: 990 477 4315 Fragments arranged by size: 4315 990 477 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4393 136 Size: 107 122 71 631 605 2721 1525 Fragments arranged by size: 2721 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5777 5777 Size: 5782 BlpI GC'TnA_GC Cuts at: 2577 3199 2577 Size: 622 5160 Bme1580I G_kGCm'C Cuts at: 1790 2270 2281 2363 2528 2666 2688 2951 3843 Size: 480 11 82 165 138 22 263 892 Cuts at: 3843 4999 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 892 480 263 165 138 82 22 11 BmgBI CAC'GTC Cuts at: 1318 2104 2836 1318 Size: 786 732 4264 Fragments arranged by size: 4264 786 732 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3513 4471 442 Size: 416 53 83 399 24 2096 958 1753 Fragments arranged by size: 2096 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5782 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2024 2149 2270 2395 2570 4442 1239 Size: 785 125 121 125 175 1872 2579 Fragments arranged by size: 2579 1872 785 175 125 125 121 Bpu10I CC'TnA_GC Cuts at: 2628 2628 Size: 5782 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3907 4772 5070 3907 Size: 865 298 4619 Fragments arranged by size: 4619 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4445 882 Size: 617 2946 2219 Fragments arranged by size: 2946 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3573 493 Size: 711 2369 2702 Fragments arranged by size: 2702 2369 711 BsaBI GATnn'nnATC Cuts at: 5776 5776 Size: 5782 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3589 3971 4606 275 Size: 53 83 186 936 2056 382 635 1451 Fragments arranged by size: 2056 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4215 4613 4887 5034 5445 4215 Size: 398 274 147 411 4552 Fragments arranged by size: 4552 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2243 2273 1178 Size: 30 165 30 840 30 4687 Fragments arranged by size: 4687 840 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2191 1186 Size: 1005 4777 BseYI C'CCAG_C Cuts at: 2599 2653 2658 4985 5481 2599 Size: 54 5 2327 496 2900 Fragments arranged by size: 2900 2327 496 54 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5209 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3232 3993 5021 1540 Size: 275 186 1231 761 1028 2301 Fragments arranged by size: 2301 1231 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3843 3928 4999 3843 Size: 85 1071 4626 Fragments arranged by size: 4626 1071 85 BsmI GAATG_Cn' Cuts at: 3293 3386 3293 Size: 93 5689 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2270 2281 2363 2473 2528 2666 2688 Size: 399 480 11 82 110 55 138 22 Cuts at: 2688 2951 3101 3843 3928 4999 1391 Size: 263 150 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 480 399 263 150 138 110 85 82 55 22 11 BspEI T'CCGG_A Cuts at: 5445 5445 Size: 5782 BspHI T'CATG_A Cuts at: 2088 3012 3671 5401 2088 Size: 924 659 1730 2469 Fragments arranged by size: 2469 1730 924 659 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3137 844 Size: 2293 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2379 3669 1394 Size: 429 556 1290 3507 Fragments arranged by size: 3507 1290 556 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2245 4277 4451 65 Size: 1183 997 2032 174 1396 Fragments arranged by size: 2032 1396 1183 997 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3066 4426 4613 1700 Size: 110 1256 1360 187 2869 Fragments arranged by size: 2869 1360 1256 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5782 BssHII G'CGCG_C Cuts at: 1925 2508 1925 Size: 583 5199 BssSI C'ACGA_G Cuts at: 2354 3842 4854 2354 Size: 1488 1012 3282 Fragments arranged by size: 3282 1488 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5782 BstBI TT'CG_AA Cuts at: 2614 4586 2614 Size: 1972 3810 BstEII G'GTnAC_C Cuts at: 1184 2074 1184 Size: 890 4892 BstYI r'GATC_y Cuts at: 1972 2931 3112 3479 3864 3881 5333 5419 5431 Size: 959 181 367 385 17 1452 86 12 Cuts at: 5431 5777 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 959 385 367 346 181 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2056 2056 Size: 5782 BtgI C'CryG_G Cuts at: 513 1065 2004 2139 2325 2496 513 Size: 552 939 135 186 171 3799 Fragments arranged by size: 3799 939 552 186 171 135 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2885 487 Size: 683 56 1659 3384 Fragments arranged by size: 3384 1659 683 56 BtsI GCAGTG_nn' Cuts at: 2430 2628 2667 2820 3298 4092 4112 2430 Size: 198 39 153 478 794 20 4100 Fragments arranged by size: 4100 794 478 198 153 39 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5782 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5747 DraI TTT'AAA Cuts at: 3086 3086 Size: 5782 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4789 809 Size: 507 3473 1802 Fragments arranged by size: 3473 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2142 3229 4118 Size: 54 1425 50 275 18 312 1087 889 Cuts at: 4118 5747 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1087 889 312 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3229 1537 Size: 275 1417 4090 Fragments arranged by size: 4090 1417 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2090 2720 3712 1353 Size: 737 630 992 3423 Fragments arranged by size: 3423 992 737 630 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4369 4743 4889 387 Size: 1064 138 2780 374 146 1280 Fragments arranged by size: 2780 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2024 2149 2270 2395 2468 2570 3845 Size: 393 392 125 121 125 73 102 1275 Cuts at: 3845 4442 5229 5497 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1275 787 597 393 392 268 125 125 121 102 73 EcoO109I rG'GnC_Cy Cuts at: 2017 2367 2662 2663 2017 Size: 350 295 1 5136 Fragments arranged by size: 5136 350 295 1 EcoRI G'AATT_C Cuts at: 3091 3469 3091 Size: 378 5404 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2977 2977 Size: 5782 FspI TGC'GCA Cuts at: 4288 4288 Size: 5782 HaeII r_GCGC'y Cuts at: 1678 2644 4929 1678 Size: 966 2285 2531 Fragments arranged by size: 2531 2285 966 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4423 4455 4497 4529 1373 Size: 32 135 32 2851 32 42 32 2626 Fragments arranged by size: 2851 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3126 3372 669 Size: 988 281 1188 246 3079 Fragments arranged by size: 3079 1188 988 281 246 HindIII A'AGCT_T Cuts at: 748 3040 748 Size: 2292 3490 HpaI GTT'AAC Cuts at: 3372 3372 Size: 5782 KpnI G_GTAC'C Cuts at: 3107 3107 Size: 5782 MfeI C'AATT_G Cuts at: 3381 3381 Size: 5782 MluI A'CGCG_T Cuts at: 5658 5658 Size: 5782 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4896 5080 1305 Size: 3591 184 2007 Fragments arranged by size: 3591 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2144 10 Size: 54 1425 343 312 3648 Fragments arranged by size: 3648 1425 343 312 54 MslI CAynn'nnrTG Cuts at: 518 1166 2396 3740 4099 4258 518 Size: 648 1230 1344 359 159 2042 Fragments arranged by size: 2042 1344 1230 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2131 2283 3198 3875 5023 5268 1606 Size: 525 152 915 677 1148 245 2120 Fragments arranged by size: 2120 1148 915 677 525 245 152 NcoI C'CATG_G Cuts at: 513 1065 2004 2139 2325 2496 513 Size: 552 939 135 186 171 3799 Fragments arranged by size: 3799 939 552 186 171 135 NdeI CA'TA_TG Cuts at: 387 387 Size: 5782 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5782 NotI GC'GGCC_GC Cuts at: 3229 3229 Size: 5782 NspI r_CATG'y Cuts at: 1132 1229 2585 2903 3140 4685 1132 Size: 97 1356 318 237 1545 2229 Fragments arranged by size: 2229 1545 1356 318 237 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5782 PciI A'CATG_T Cuts at: 4681 4681 Size: 5782 PflMI CCAn_nnn'nTGG Cuts at: 1108 2047 2172 1108 Size: 939 125 4718 Fragments arranged by size: 4718 939 125 PfoI T'CCnGG_A Cuts at: 2247 2247 Size: 5782 PmeI GTTT'AAAC Cuts at: 3086 3086 Size: 5782 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3851 3883 5364 5396 3851 Size: 32 1481 32 4237 Fragments arranged by size: 4237 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2017 2367 2017 Size: 350 5432 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5782 PsiI TTA'TAA Cuts at: 3352 3352 Size: 5782 PspOMI G'GGCC_C Cuts at: 2662 2662 Size: 5782 PstI C_TGCA'G Cuts at: 830 2124 2574 2623 2815 3134 830 Size: 1294 450 49 192 319 3478 Fragments arranged by size: 3478 1294 450 319 192 49 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5782 SalI G'TCGA_C Cuts at: 1936 3124 1936 Size: 1188 4594 SapI GCTCTTCn'nnn_ Cuts at: 2720 2720 Size: 5782 SbfI CC_TGCA'GG Cuts at: 3134 3134 Size: 5782 ScaI AGT'ACT Cuts at: 4030 5742 4030 Size: 1712 4070 SexAI A'CCwGG_T Cuts at: 2291 2291 Size: 5782 SfcI C'TryA_G Cuts at: 826 1045 2120 2570 2619 2811 3130 4265 4946 Size: 219 1075 450 49 192 319 1135 681 Cuts at: 4946 5137 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1075 681 450 319 219 192 191 49 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5782 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5782 SmaI CCC'GGG Cuts at: 1872 3109 1872 Size: 1237 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3886 4787 5049 820 Size: 197 926 1943 901 262 1553 Fragments arranged by size: 1943 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3573 493 Size: 3080 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5782 SphI G_CATG'C Cuts at: 1132 3140 1132 Size: 2008 3774 SspI AAT'ATT Cuts at: 5 52 3706 5 Size: 47 3654 2081 Fragments arranged by size: 3654 2081 47 StuI AGG'CCT Cuts at: 1517 2453 1517 Size: 936 4846 StyI C'CwwG_G Cuts at: 513 1065 2004 2139 2325 2496 2952 3221 513 Size: 552 939 135 186 171 456 269 3074 Fragments arranged by size: 3074 939 552 456 269 186 171 135 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2509 2591 3809 3826 3979 4164 4609 Size: 1255 929 82 1218 17 153 185 445 Cuts at: 4609 325 Size: 1498 Fragments arranged by size: 1498 1255 1218 929 445 185 153 82 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2700 4028 5740 Size: 274 80 33 51 1443 723 1328 1712 Cuts at: 5740 96 Size: 138 Fragments arranged by size: 1712 1443 1328 723 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5742 5774 5742 Size: 32 5750 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5782 XbaI T'CTAG_A Cuts at: 3118 3118 Size: 5782 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5782 XmaI C'CCGG_G Cuts at: 1870 3107 1870 Size: 1237 4545 XmnI GAAnn'nnTTC Cuts at: 2453 3911 2453 Size: 1458 4324 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3590 276 Size: 53 83 186 936 2056 2468 Fragments arranged by size: 2468 2056 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI