(Circular) (Six-base) MAPSORT of: pFN21AB6413.seq Check: 3142 from: 1 to: 5629 pFN21AB6413 5629 bp With 182 enzymes: SgfI * October 20, 2009 10:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2512 2512 Size: 5629 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2580 3439 278 Size: 53 83 186 936 1044 859 2468 Fragments arranged by size: 2468 1044 936 859 186 83 53 AccI GT'mk_AC Cuts at: 1937 2874 2972 1937 Size: 937 98 4594 Fragments arranged by size: 4594 937 98 Acc65I G'GTAC_C Cuts at: 2950 2950 Size: 5629 AclI AA'CG_TT Cuts at: 1563 3756 4129 1563 Size: 2193 373 3063 Fragments arranged by size: 3063 2193 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2294 2905 3692 5076 5344 1632 Size: 662 611 787 1384 268 1917 Fragments arranged by size: 1917 1384 787 662 611 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5432 AflIII A'CryG_T Cuts at: 1203 2334 4528 5505 1203 Size: 1131 2194 977 1327 Fragments arranged by size: 2194 1327 1131 977 AgeI A'CCGG_T Cuts at: 4460 4460 Size: 5629 AhdI GACnn_n'nnGTC Cuts at: 4358 4358 Size: 5629 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5629 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5629 AlwNI CAG_nnn'CTG Cuts at: 2818 3372 4944 2818 Size: 554 1572 3503 Fragments arranged by size: 3503 1572 554 ApaI G_GGCC'C Cuts at: 2590 2590 Size: 5629 ApaLI G'TGCA_C Cuts at: 3686 4842 3686 Size: 1156 4473 ApoI r'AATT_y Cuts at: 1493 2938 3164 3316 5425 1493 Size: 1445 226 152 2109 1697 Fragments arranged by size: 2109 1697 1445 226 152 AseI AT'TA_AT Cuts at: 160 1032 4183 160 Size: 872 3151 1606 Fragments arranged by size: 3151 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5629 AvaI C'yCGr_G Cuts at: 1870 1943 2426 2954 1870 Size: 73 483 528 4545 Fragments arranged by size: 4545 528 483 73 BamHI G'GATC_C Cuts at: 2959 3326 2959 Size: 367 5262 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2085 2710 2950 4405 618 Size: 325 217 627 298 625 240 1455 1842 Fragments arranged by size: 1842 1455 627 625 325 298 240 217 BanII G_rGCy'C Cuts at: 1391 2048 2590 2948 1391 Size: 657 542 358 4072 Fragments arranged by size: 4072 657 542 358 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4658 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3820 3854 1903 Size: 34 1883 34 3678 Fragments arranged by size: 3678 1883 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3521 4731 3521 Size: 1210 4419 BclI T'GATC_A Cuts at: 1548 1548 Size: 5629 BfrBI ATG'CAT Cuts at: 2848 2848 Size: 5629 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4240 136 Size: 107 122 71 631 605 2568 1525 Fragments arranged by size: 2568 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5624 5624 Size: 5629 BlpI GC'TnA_GC Cuts at: 3046 3046 Size: 5629 Bme1580I G_kGCm'C Cuts at: 1790 2590 3690 4846 1790 Size: 800 1100 1156 2573 Fragments arranged by size: 2573 1156 1100 800 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5629 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2640 2788 2851 Size: 416 53 83 399 24 1223 148 63 Cuts at: 2851 2887 3360 4318 442 Size: 36 473 958 1753 Fragments arranged by size: 1753 1223 958 473 416 399 148 83 63 53 36 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5629 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2153 4289 1239 Size: 914 2136 2579 Fragments arranged by size: 2579 2136 914 Bpu10I CC'TnA_GC Cuts at: 2238 2661 2238 Size: 423 5206 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2000 2138 2749 3754 4619 4917 2000 Size: 138 611 1005 865 298 2712 Fragments arranged by size: 2712 1005 865 611 298 138 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4292 882 Size: 617 2793 2219 Fragments arranged by size: 2793 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3420 493 Size: 711 2216 2702 Fragments arranged by size: 2702 2216 711 BsaBI GATnn'nnATC Cuts at: 5623 5623 Size: 5629 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2577 3436 3818 4453 Size: 53 83 186 936 1044 859 382 635 Cuts at: 4453 275 Size: 1451 Fragments arranged by size: 1451 1044 936 859 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2040 4062 4460 4734 4881 5292 2040 Size: 2022 398 274 147 411 2377 Fragments arranged by size: 2377 2022 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2336 2366 2562 2592 1178 Size: 30 165 30 933 30 196 30 4215 Fragments arranged by size: 4215 933 196 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2210 2666 1186 Size: 1024 456 4149 Fragments arranged by size: 4149 1024 456 BseYI C'CCAG_C Cuts at: 2048 2067 4832 5328 2048 Size: 19 2765 496 2349 Fragments arranged by size: 2765 2349 496 19 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2431 2584 1163 Size: 573 695 153 4208 Fragments arranged by size: 4208 695 573 153 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2208 3079 3840 4868 1540 Size: 275 186 207 871 761 1028 2301 Fragments arranged by size: 2301 1028 871 761 275 207 186 BsiHKAI G_wGCw'C Cuts at: 3690 3775 4846 3690 Size: 85 1071 4473 Fragments arranged by size: 4473 1071 85 BsmI GAATG_Cn' Cuts at: 3140 3233 3140 Size: 93 5536 BsmBI CGTCTCn'nnnn_ Cuts at: 2117 2117 Size: 5629 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2048 2590 2948 3690 3775 4846 1391 Size: 399 258 542 358 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 542 399 358 258 85 BspEI T'CCGG_A Cuts at: 2040 5292 2040 Size: 3252 2377 BspHI T'CATG_A Cuts at: 3518 5248 3518 Size: 1730 3899 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2512 2646 2984 844 Size: 1668 134 338 3489 Fragments arranged by size: 3489 1668 338 134 BsrBI CCG'CTC Cuts at: 1394 1823 3516 1394 Size: 429 1693 3507 Fragments arranged by size: 3507 1693 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4124 4298 65 Size: 1183 2876 174 1396 Fragments arranged by size: 2876 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2082 4273 4460 1700 Size: 110 272 2191 187 2869 Fragments arranged by size: 2869 2191 272 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5629 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5629 BssSI C'ACGA_G Cuts at: 3689 4701 3689 Size: 1012 4617 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5629 BstBI TT'CG_AA Cuts at: 4433 4433 Size: 5629 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5629 BstXI CCAn_nnnn'nTGG Cuts at: 2701 2701 Size: 5629 BstYI r'GATC_y Cuts at: 1972 2349 2959 3326 3711 3728 5180 5266 5278 Size: 377 610 367 385 17 1452 86 12 Cuts at: 5278 5624 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 610 385 377 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2811 2811 Size: 5629 BtgI C'CryG_G Cuts at: 513 1065 2004 2285 2676 2714 513 Size: 552 939 281 391 38 3428 Fragments arranged by size: 3428 939 552 391 281 38 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2383 487 Size: 683 56 1157 3733 Fragments arranged by size: 3733 1157 683 56 BtsI GCAGTG_nn' Cuts at: 2746 3145 3939 3959 2746 Size: 399 794 20 4416 Fragments arranged by size: 4416 794 399 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5629 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2709 2744 529 Size: 35 2145 35 3414 Fragments arranged by size: 3414 2145 35 35 DraI TTT'AAA Cuts at: 2933 2933 Size: 5629 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4636 809 Size: 507 3320 1802 Fragments arranged by size: 3320 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2055 2080 2205 Size: 54 1425 50 275 18 225 25 125 Cuts at: 2205 2717 3076 3965 5594 8 Size: 512 359 889 1629 43 Fragments arranged by size: 1629 1425 889 512 359 275 225 125 54 50 43 25 18 EagI C'GGCC_G Cuts at: 1537 1812 2205 3076 1537 Size: 275 393 871 4090 Fragments arranged by size: 4090 871 393 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2529 2540 2609 2924 3559 1353 Size: 1176 11 69 315 635 3423 Fragments arranged by size: 3423 1176 635 315 69 11 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4216 4590 4736 387 Size: 1064 138 2627 374 146 1280 Fragments arranged by size: 2627 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2153 2294 2905 3692 4289 5076 5344 Size: 393 521 141 611 787 597 787 268 Cuts at: 5344 1239 Size: 1524 Fragments arranged by size: 1524 787 787 611 597 521 393 268 141 EcoO109I rG'GnC_Cy Cuts at: 2586 2665 2815 2586 Size: 79 150 5400 Fragments arranged by size: 5400 150 79 EcoRI G'AATT_C Cuts at: 2938 3316 2938 Size: 378 5251 FspI TGC'GCA Cuts at: 4135 4135 Size: 5629 HaeII r_GCGC'y Cuts at: 1678 2036 2688 4776 1678 Size: 358 652 2088 2531 Fragments arranged by size: 2531 2088 652 358 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2186 2218 2321 2353 2562 Size: 32 135 32 614 32 103 32 209 Cuts at: 2562 2594 2794 2826 4270 4302 4344 4376 1373 Size: 32 200 32 1444 32 42 32 2626 Fragments arranged by size: 2626 1444 614 209 200 135 103 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2875 2973 3219 669 Size: 988 281 937 98 246 3079 Fragments arranged by size: 3079 988 937 281 246 98 HindIII A'AGCT_T Cuts at: 748 748 Size: 5629 HpaI GTT'AAC Cuts at: 3219 3219 Size: 5629 KpnI G_GTAC'C Cuts at: 2954 2954 Size: 5629 MfeI C'AATT_G Cuts at: 3228 3228 Size: 5629 MluI A'CGCG_T Cuts at: 5505 5505 Size: 5629 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4743 4927 1305 Size: 3438 184 2007 Fragments arranged by size: 3438 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2057 10 Size: 54 1425 343 225 3582 Fragments arranged by size: 3582 1425 343 225 54 MslI CAynn'nnrTG Cuts at: 518 1166 2699 3587 3946 4105 518 Size: 648 1533 888 359 159 2042 Fragments arranged by size: 2042 1533 888 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2671 2716 2898 3045 3722 4870 5115 1606 Size: 1065 45 182 147 677 1148 245 2120 Fragments arranged by size: 2120 1148 1065 677 245 182 147 45 NaeI GCC'GGC Cuts at: 2084 2084 Size: 5629 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4138 Fragments arranged by size: 4138 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5629 NgoMIV G'CCGG_C Cuts at: 2082 2082 Size: 5629 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5629 NotI GC'GGCC_GC Cuts at: 3076 3076 Size: 5629 NsiI A_TGCA'T Cuts at: 2850 2850 Size: 5629 NspI r_CATG'y Cuts at: 1132 1229 2848 2987 4532 1132 Size: 97 1619 139 1545 2229 Fragments arranged by size: 2229 1619 1545 139 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5629 PciI A'CATG_T Cuts at: 4528 4528 Size: 5629 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5629 PfoI T'CCnGG_A Cuts at: 2817 2817 Size: 5629 PmeI GTTT'AAAC Cuts at: 2933 2933 Size: 5629 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3698 3730 5211 5243 3698 Size: 32 1481 32 4084 Fragments arranged by size: 4084 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2815 2815 Size: 5629 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5629 PsiI TTA'TAA Cuts at: 3199 3199 Size: 5629 PspOMI G'GGCC_C Cuts at: 2586 2586 Size: 5629 PspXI vC'TCGA_Gb Cuts at: 2426 2426 Size: 5629 PstI C_TGCA'G Cuts at: 830 2262 2981 830 Size: 1432 719 3478 Fragments arranged by size: 3478 1432 719 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5629 SacII CC_GC'GG Cuts at: 2717 2717 Size: 5629 SalI G'TCGA_C Cuts at: 1936 2873 2971 1936 Size: 937 98 4594 Fragments arranged by size: 4594 937 98 SapI GCTCTTCn'nnn_ Cuts at: 2609 2609 Size: 5629 SbfI CC_TGCA'GG Cuts at: 2981 2981 Size: 5629 ScaI AGT'ACT Cuts at: 3877 5589 3877 Size: 1712 3917 SfcI C'TryA_G Cuts at: 826 1045 2232 2258 2977 4112 4793 4984 826 Size: 219 1187 26 719 1135 681 191 1471 Fragments arranged by size: 1471 1187 1135 719 681 219 191 26 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5629 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5629 SmaI CCC'GGG Cuts at: 1872 2956 1872 Size: 1084 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2015 2153 2426 2728 3733 4634 Size: 197 926 72 138 273 302 1005 901 Cuts at: 4634 4896 820 Size: 262 1553 Fragments arranged by size: 1553 1005 926 901 302 273 262 197 138 72 SnaBI TAC'GTA Cuts at: 493 3420 493 Size: 2927 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5629 SphI G_CATG'C Cuts at: 1132 2987 1132 Size: 1855 3774 SspI AAT'ATT Cuts at: 5 52 3553 5 Size: 47 3501 2081 Fragments arranged by size: 3501 2081 47 StuI AGG'CCT Cuts at: 1517 2495 1517 Size: 978 4651 StyI C'CwwG_G Cuts at: 513 1065 2004 2187 2529 3068 513 Size: 552 939 183 342 539 3074 Fragments arranged by size: 3074 939 552 539 342 183 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3656 3673 3826 4011 4456 325 Size: 1255 2076 17 153 185 445 1498 Fragments arranged by size: 2076 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3875 5587 96 Size: 274 80 33 51 1443 1898 1712 138 Fragments arranged by size: 1898 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5589 5621 5589 Size: 32 5597 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5629 XbaI T'CTAG_A Cuts at: 2965 2965 Size: 5629 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2458 2458 Size: 5629 XhoI C'TCGA_G Cuts at: 1943 2426 1943 Size: 483 5146 XmaI C'CCGG_G Cuts at: 1870 2954 1870 Size: 1084 4545 XmnI GAAnn'nnTTC Cuts at: 3758 3758 Size: 5629 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2578 3437 276 Size: 53 83 186 936 1044 859 2468 Fragments arranged by size: 2468 1044 936 859 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI PacI PmlI PsrI PvuII RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI