(Circular) (Six-base) MAPSORT of: pFN21AE0876.seq Check: 4265 from: 1 to: 5743 pFN21AE0876 5743 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2075 2075 Size: 5743 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3553 278 Size: 53 83 186 936 2017 2468 Fragments arranged by size: 2468 2017 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3086 1937 Size: 1149 4594 Acc65I G'GTAC_C Cuts at: 3064 3064 Size: 5743 AclI AA'CG_TT Cuts at: 1563 3870 4243 1563 Size: 2307 373 3063 Fragments arranged by size: 3063 2307 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3806 5190 5458 1632 Size: 2174 1384 268 1917 Fragments arranged by size: 2174 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5546 AflIII A'CryG_T Cuts at: 1203 4642 5619 1203 Size: 3439 977 1327 Fragments arranged by size: 3439 1327 977 AgeI A'CCGG_T Cuts at: 4574 4574 Size: 5743 AhdI GACnn_n'nnGTC Cuts at: 4472 4472 Size: 5743 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5743 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2550 3041 1184 Size: 1366 491 3886 Fragments arranged by size: 3886 1366 491 AlwNI CAG_nnn'CTG Cuts at: 3486 5058 3486 Size: 1572 4171 ApaLI G'TGCA_C Cuts at: 3800 4956 3800 Size: 1156 4587 ApoI r'AATT_y Cuts at: 1493 2132 2640 3052 3278 3430 5539 1493 Size: 639 508 412 226 152 2109 1697 Fragments arranged by size: 2109 1697 639 508 412 226 152 AseI AT'TA_AT Cuts at: 160 1032 2784 4297 160 Size: 872 1752 1513 1606 Fragments arranged by size: 1752 1606 1513 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5743 AvaI C'yCGr_G Cuts at: 1870 1943 3068 1870 Size: 73 1125 4545 Fragments arranged by size: 4545 1125 73 BamHI G'GATC_C Cuts at: 3073 3440 3073 Size: 367 5376 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3064 4519 618 Size: 325 217 627 1277 1455 1842 Fragments arranged by size: 1842 1455 1277 627 325 217 BanII G_rGCy'C Cuts at: 1391 2169 2426 3062 1391 Size: 778 257 636 4072 Fragments arranged by size: 4072 778 636 257 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4772 BbvCI CC'TCA_GC Cuts at: 2793 2793 Size: 5743 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3934 3968 1903 Size: 34 1997 34 3678 Fragments arranged by size: 3678 1997 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3635 4845 3635 Size: 1210 4533 BclI T'GATC_A Cuts at: 1548 2623 1548 Size: 1075 4668 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4354 136 Size: 107 122 71 631 605 2682 1525 Fragments arranged by size: 2682 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5738 5738 Size: 5743 BlpI GC'TnA_GC Cuts at: 3160 3160 Size: 5743 Bme1580I G_kGCm'C Cuts at: 1790 3804 4960 1790 Size: 2014 1156 2573 Fragments arranged by size: 2573 2014 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5743 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3474 4432 442 Size: 416 53 83 399 24 2057 958 1753 Fragments arranged by size: 2057 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5743 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2153 4403 1239 Size: 914 2250 2579 Fragments arranged by size: 2579 2250 914 Bpu10I CC'TnA_GC Cuts at: 2793 3014 2793 Size: 221 5522 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3868 4733 5031 3868 Size: 865 298 4580 Fragments arranged by size: 4580 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4406 882 Size: 617 2907 2219 Fragments arranged by size: 2907 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3534 493 Size: 711 2330 2702 Fragments arranged by size: 2702 2330 711 BsaBI GATnn'nnATC Cuts at: 5737 5737 Size: 5743 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3550 3932 4567 275 Size: 53 83 186 936 2017 382 635 1451 Fragments arranged by size: 2017 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4176 4574 4848 4995 5406 4176 Size: 398 274 147 411 4513 Fragments arranged by size: 4513 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5518 Fragments arranged by size: 5518 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2222 1186 Size: 1036 4707 BseYI C'CCAG_C Cuts at: 2076 4946 5442 2076 Size: 2870 496 2377 Fragments arranged by size: 2870 2377 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5170 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3193 3954 4982 1540 Size: 275 186 1192 761 1028 2301 Fragments arranged by size: 2301 1192 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2169 2395 3804 3889 4960 2169 Size: 226 1409 85 1071 2952 Fragments arranged by size: 2952 1409 1071 226 85 BsmI GAATG_Cn' Cuts at: 3254 3347 3254 Size: 93 5650 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2169 2395 2426 3062 3804 3889 4960 Size: 399 379 226 31 636 742 85 1071 Cuts at: 4960 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 636 399 379 226 85 31 BspEI T'CCGG_A Cuts at: 5406 5406 Size: 5743 BspHI T'CATG_A Cuts at: 2505 2694 3632 5362 2505 Size: 189 938 1730 2886 Fragments arranged by size: 2886 1730 938 189 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2075 3098 844 Size: 1231 1023 3489 Fragments arranged by size: 3489 1231 1023 BsrBI CCG'CTC Cuts at: 1394 1823 3630 1394 Size: 429 1807 3507 Fragments arranged by size: 3507 1807 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4238 4412 65 Size: 1183 2990 174 1396 Fragments arranged by size: 2990 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4387 4574 1700 Size: 110 2577 187 2869 Fragments arranged by size: 2869 2577 187 110 BsrGI T'GTAC_A Cuts at: 96 2007 2202 2874 96 Size: 1911 195 672 2965 Fragments arranged by size: 2965 1911 672 195 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5743 BssSI C'ACGA_G Cuts at: 3803 4815 3803 Size: 1012 4731 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5743 BstBI TT'CG_AA Cuts at: 4547 4547 Size: 5743 BstEII G'GTnAC_C Cuts at: 1184 2087 1184 Size: 903 4840 BstYI r'GATC_y Cuts at: 1972 3073 3440 3825 3842 5294 5380 5392 5738 Size: 1101 367 385 17 1452 86 12 346 Cuts at: 5738 1972 Size: 1977 Fragments arranged by size: 1977 1452 1101 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2975 2975 Size: 5743 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5191 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5004 Fragments arranged by size: 5004 683 56 BtsI GCAGTG_nn' Cuts at: 3259 4053 4073 3259 Size: 794 20 4929 Fragments arranged by size: 4929 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5743 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5708 DraI TTT'AAA Cuts at: 3047 3047 Size: 5743 DraIII CAC_nnn'GTG Cuts at: 2433 2470 2433 Size: 37 5706 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4750 809 Size: 507 3434 1802 Fragments arranged by size: 3434 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2472 3190 4079 Size: 54 1425 50 275 18 642 718 889 Cuts at: 4079 5708 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 718 642 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3190 1537 Size: 275 1378 4090 Fragments arranged by size: 4090 1378 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2773 3673 1353 Size: 1420 900 3423 Fragments arranged by size: 3423 1420 900 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4330 4704 4850 387 Size: 1064 138 2741 374 146 1280 Fragments arranged by size: 2741 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2167 2167 Size: 5743 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2153 3806 4403 5190 5458 1239 Size: 393 521 1653 597 787 268 1524 Fragments arranged by size: 1653 1524 787 597 521 393 268 EcoRI G'AATT_C Cuts at: 2132 3052 3430 2132 Size: 920 378 4445 Fragments arranged by size: 4445 920 378 EcoRV GAT'ATC Cuts at: 2674 2674 Size: 5743 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2827 2827 Size: 5743 FspI TGC'GCA Cuts at: 4249 4249 Size: 5743 HaeII r_GCGC'y Cuts at: 1678 4890 1678 Size: 3212 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2354 2386 3007 3039 4384 Size: 32 135 32 782 32 621 32 1345 Cuts at: 4384 4416 4458 4490 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1345 782 621 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3087 3333 669 Size: 988 281 1149 246 3079 Fragments arranged by size: 3079 1149 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5743 HpaI GTT'AAC Cuts at: 3333 3333 Size: 5743 KpnI G_GTAC'C Cuts at: 3068 3068 Size: 5743 MfeI C'AATT_G Cuts at: 3342 3342 Size: 5743 MluI A'CGCG_T Cuts at: 5619 5619 Size: 5743 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4857 5041 1305 Size: 3552 184 2007 Fragments arranged by size: 3552 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2474 10 Size: 54 1425 343 642 3279 Fragments arranged by size: 3279 1425 642 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2204 2504 2684 2699 3701 4060 4219 Size: 648 1038 300 180 15 1002 359 159 Cuts at: 4219 518 Size: 2042 Fragments arranged by size: 2042 1038 1002 648 359 300 180 159 15 MspA1I CmG'CkG Cuts at: 1606 3159 3836 4984 5229 1606 Size: 1553 677 1148 245 2120 Fragments arranged by size: 2120 1553 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5191 NdeI CA'TA_TG Cuts at: 387 387 Size: 5743 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5743 NotI GC'GGCC_GC Cuts at: 3190 3190 Size: 5743 NspI r_CATG'y Cuts at: 1132 1229 3101 4646 1132 Size: 97 1872 1545 2229 Fragments arranged by size: 2229 1872 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5743 PciI A'CATG_T Cuts at: 4642 4642 Size: 5743 PflMI CCAn_nnn'nTGG Cuts at: 1108 2470 1108 Size: 1362 4381 PfoI T'CCnGG_A Cuts at: 2543 2543 Size: 5743 PmeI GTTT'AAAC Cuts at: 3047 3047 Size: 5743 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3812 3844 5325 5357 3812 Size: 32 1481 32 4198 Fragments arranged by size: 4198 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5743 PsiI TTA'TAA Cuts at: 3313 3313 Size: 5743 PstI C_TGCA'G Cuts at: 830 2400 3095 830 Size: 1570 695 3478 Fragments arranged by size: 3478 1570 695 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5743 SacI G_AGCT'C Cuts at: 2169 2169 Size: 5743 SalI G'TCGA_C Cuts at: 1936 3085 1936 Size: 1149 4594 SbfI CC_TGCA'GG Cuts at: 3095 3095 Size: 5743 ScaI AGT'ACT Cuts at: 3991 5703 3991 Size: 1712 4031 SfcI C'TryA_G Cuts at: 826 1045 2396 2968 3091 4226 4907 5098 826 Size: 219 1351 572 123 1135 681 191 1471 Fragments arranged by size: 1471 1351 1135 681 572 219 191 123 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5743 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5743 SmaI CCC'GGG Cuts at: 1872 3070 1872 Size: 1198 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3847 4748 5010 820 Size: 197 926 1904 901 262 1553 Fragments arranged by size: 1904 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3534 493 Size: 3041 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5743 SphI G_CATG'C Cuts at: 1132 3101 1132 Size: 1969 3774 SspI AAT'ATT Cuts at: 5 52 2710 3667 5 Size: 47 2658 957 2081 Fragments arranged by size: 2658 2081 957 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5743 StyI C'CwwG_G Cuts at: 513 1065 2413 3182 513 Size: 552 1348 769 3074 Fragments arranged by size: 3074 1348 769 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3770 3787 3940 4125 4570 325 Size: 1255 2190 17 153 185 445 1498 Fragments arranged by size: 2190 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2007 2202 2874 Size: 274 80 33 51 1443 30 195 672 Cuts at: 2874 3989 5701 96 Size: 1115 1712 138 Fragments arranged by size: 1712 1443 1115 672 274 195 138 80 51 33 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5703 5735 5703 Size: 32 5711 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5743 XbaI T'CTAG_A Cuts at: 3079 3079 Size: 5743 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5743 XmaI C'CCGG_G Cuts at: 1870 3068 1870 Size: 1198 4545 XmnI GAAnn'nnTTC Cuts at: 2136 2810 3872 2136 Size: 674 1062 4007 Fragments arranged by size: 4007 1062 674 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3551 276 Size: 53 83 186 936 2017 2468 Fragments arranged by size: 2468 2017 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI