(Circular) (Six-base) MAPSORT of: pFN21AB6342.seq Check: 8646 from: 1 to: 5578 pFN21AB6342 5578 bp With 182 enzymes: SgfI * October 20, 2009 10:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2695 2695 Size: 5578 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3388 278 Size: 53 83 186 936 1852 2468 Fragments arranged by size: 2468 1852 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2921 1937 Size: 984 4594 Acc65I G'GTAC_C Cuts at: 2899 2899 Size: 5578 AclI AA'CG_TT Cuts at: 1563 3705 4078 1563 Size: 2142 373 3063 Fragments arranged by size: 3063 2142 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3641 5025 5293 1632 Size: 2009 1384 268 1917 Fragments arranged by size: 2009 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5381 AflIII A'CryG_T Cuts at: 1203 4477 5454 1203 Size: 3274 977 1327 Fragments arranged by size: 3274 1327 977 AgeI A'CCGG_T Cuts at: 4409 4409 Size: 5578 AhdI GACnn_n'nnGTC Cuts at: 4307 4307 Size: 5578 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5578 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5578 AlwNI CAG_nnn'CTG Cuts at: 2644 3321 4893 2644 Size: 677 1572 3329 Fragments arranged by size: 3329 1572 677 ApaLI G'TGCA_C Cuts at: 3635 4791 3635 Size: 1156 4422 ApoI r'AATT_y Cuts at: 1493 2887 3113 3265 5374 1493 Size: 1394 226 152 2109 1697 Fragments arranged by size: 2109 1697 1394 226 152 AseI AT'TA_AT Cuts at: 160 1032 4132 160 Size: 872 3100 1606 Fragments arranged by size: 3100 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5578 AvaI C'yCGr_G Cuts at: 1870 1943 2116 2903 1870 Size: 73 173 787 4545 Fragments arranged by size: 4545 787 173 73 BamHI G'GATC_C Cuts at: 2908 3275 2908 Size: 367 5211 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2782 2899 4354 618 Size: 325 217 627 995 117 1455 1842 Fragments arranged by size: 1842 1455 995 627 325 217 117 BanII G_rGCy'C Cuts at: 1391 2850 2897 1391 Size: 1459 47 4072 Fragments arranged by size: 4072 1459 47 BbeI G_GCGC'C Cuts at: 2786 2786 Size: 5578 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2829 928 Size: 971 930 3677 Fragments arranged by size: 3677 971 930 BbvCI CC'TCA_GC Cuts at: 2460 2805 2460 Size: 345 5233 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3769 3803 1903 Size: 34 1832 34 3678 Fragments arranged by size: 3678 1832 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3470 4680 3470 Size: 1210 4368 BclI T'GATC_A Cuts at: 1548 1548 Size: 5578 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4189 136 Size: 107 122 71 631 605 2517 1525 Fragments arranged by size: 2517 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5573 5573 Size: 5578 BlpI GC'TnA_GC Cuts at: 2432 2995 2432 Size: 563 5015 Bme1580I G_kGCm'C Cuts at: 1790 2030 2067 3639 4795 1790 Size: 240 37 1572 1156 2573 Fragments arranged by size: 2573 1572 1156 240 37 BmgBI CAC'GTC Cuts at: 1318 2875 1318 Size: 1557 4021 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3309 4267 442 Size: 416 53 83 399 24 1892 958 1753 Fragments arranged by size: 1892 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5578 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2741 4238 1239 Size: 1502 1497 2579 Fragments arranged by size: 2579 1502 1497 Bpu10I CC'TnA_GC Cuts at: 2180 2460 2805 2180 Size: 280 345 4953 Fragments arranged by size: 4953 345 280 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3703 4568 4866 3703 Size: 865 298 4415 Fragments arranged by size: 4415 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4241 882 Size: 617 2742 2219 Fragments arranged by size: 2742 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2755 3369 493 Size: 711 1551 614 2702 Fragments arranged by size: 2702 1551 711 614 BsaBI GATnn'nnATC Cuts at: 5572 5572 Size: 5578 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2318 2783 3385 3767 Size: 53 83 186 936 785 465 602 382 Cuts at: 3767 4402 275 Size: 635 1451 Fragments arranged by size: 1451 936 785 635 602 465 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4011 4409 4683 4830 5241 4011 Size: 398 274 147 411 4348 Fragments arranged by size: 4348 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2243 2273 1178 Size: 30 165 30 840 30 4483 Fragments arranged by size: 4483 840 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2248 2477 2738 2774 2843 2846 2849 2852 Size: 1062 229 261 36 69 3 3 3 Cuts at: 2852 2855 2858 1186 Size: 3 3 3906 Fragments arranged by size: 3906 1062 261 229 69 36 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2046 2360 4781 5277 2046 Size: 314 2421 496 2347 Fragments arranged by size: 2421 2347 496 314 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2503 2543 2720 1163 Size: 573 767 40 177 4021 Fragments arranged by size: 4021 767 573 177 40 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2088 2598 3028 3789 4817 1540 Size: 275 186 87 510 430 761 1028 2301 Fragments arranged by size: 2301 1028 761 510 430 275 186 87 BsiHKAI G_wGCw'C Cuts at: 2186 2874 3639 3724 4795 2186 Size: 688 765 85 1071 2969 Fragments arranged by size: 2969 1071 765 688 85 BsmI GAATG_Cn' Cuts at: 3089 3182 3089 Size: 93 5485 BsmBI CGTCTCn'nnnn_ Cuts at: 2522 2522 Size: 5578 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2030 2067 2186 2850 2874 2897 3639 Size: 399 240 37 119 664 24 23 742 Cuts at: 3639 3724 4795 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 664 399 240 119 85 37 24 23 BspEI T'CCGG_A Cuts at: 5241 5241 Size: 5578 BspHI T'CATG_A Cuts at: 3467 5197 3467 Size: 1730 3848 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2695 2933 844 Size: 1851 238 3489 Fragments arranged by size: 3489 1851 238 BsrBI CCG'CTC Cuts at: 1394 1823 2011 2121 2436 3465 1394 Size: 429 188 110 315 1029 3507 Fragments arranged by size: 3507 1029 429 315 188 110 BsrDI GCAATG_nn' Cuts at: 65 1248 2454 4073 4247 65 Size: 1183 1206 1619 174 1396 Fragments arranged by size: 1619 1396 1206 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2087 2335 4222 4409 1700 Size: 110 277 248 1887 187 2869 Fragments arranged by size: 2869 1887 277 248 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5578 BssHII G'CGCG_C Cuts at: 1925 2153 1925 Size: 228 5350 BssSI C'ACGA_G Cuts at: 3638 4650 3638 Size: 1012 4566 BstAPI GCAn_nnn'nTGC Cuts at: 794 2707 794 Size: 1913 3665 BstBI TT'CG_AA Cuts at: 4382 4382 Size: 5578 BstEII G'GTnAC_C Cuts at: 1184 2338 2662 1184 Size: 1154 324 4100 Fragments arranged by size: 4100 1154 324 BstYI r'GATC_y Cuts at: 1972 2908 3275 3660 3677 5129 5215 5227 5573 Size: 936 367 385 17 1452 86 12 346 Cuts at: 5573 1972 Size: 1977 Fragments arranged by size: 1977 1452 936 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2172 2478 2601 513 Size: 552 939 168 306 123 3490 Fragments arranged by size: 3490 939 552 306 168 123 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2530 487 Size: 683 56 1304 3535 Fragments arranged by size: 3535 1304 683 56 BtsI GCAGTG_nn' Cuts at: 3094 3888 3908 3094 Size: 794 20 4764 Fragments arranged by size: 4764 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5578 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5543 DraI TTT'AAA Cuts at: 2882 2882 Size: 5578 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2536 4585 809 Size: 507 1220 2049 1802 Fragments arranged by size: 2049 1802 1220 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2085 2089 2541 Size: 54 1425 50 275 18 255 4 452 Cuts at: 2541 2595 2736 3025 3914 5543 8 Size: 54 141 289 889 1629 43 Fragments arranged by size: 1629 1425 889 452 289 275 255 141 54 54 50 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 2085 2595 3025 1537 Size: 275 273 510 430 4090 Fragments arranged by size: 4090 510 430 275 273 EarI CTCTTCn'nnn_ Cuts at: 1353 2009 2420 2685 3508 1353 Size: 656 411 265 823 3423 Fragments arranged by size: 3423 823 656 411 265 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4165 4539 4685 387 Size: 1064 138 2576 374 146 1280 Fragments arranged by size: 2576 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2741 3641 4238 5025 5293 1239 Size: 393 1109 900 597 787 268 1524 Fragments arranged by size: 1524 1109 900 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2489 2489 Size: 5578 EcoO109I rG'GnC_Cy Cuts at: 2494 2510 2494 Size: 16 5562 EcoRI G'AATT_C Cuts at: 2887 3265 2887 Size: 378 5200 FseI GG_CCGG'CC Cuts at: 2091 2091 Size: 5578 FspI TGC'GCA Cuts at: 4084 4084 Size: 5578 HaeII r_GCGC'y Cuts at: 1678 2036 2300 2556 2732 2786 4725 1678 Size: 358 264 256 176 54 1939 2531 Fragments arranged by size: 2531 1939 358 264 256 176 54 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4219 4251 4293 4325 1373 Size: 32 135 32 2647 32 42 32 2626 Fragments arranged by size: 2647 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2922 3168 669 Size: 988 281 984 246 3079 Fragments arranged by size: 3079 988 984 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5578 HpaI GTT'AAC Cuts at: 3168 3168 Size: 5578 KasI G'GCGC_C Cuts at: 2782 2782 Size: 5578 KpnI G_GTAC'C Cuts at: 2903 2903 Size: 5578 MfeI C'AATT_G Cuts at: 3177 3177 Size: 5578 MluI A'CGCG_T Cuts at: 5454 5454 Size: 5578 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2286 4692 4876 1305 Size: 981 2406 184 2007 Fragments arranged by size: 2406 2007 981 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3756 Fragments arranged by size: 3756 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2097 3536 3895 4054 518 Size: 648 931 1439 359 159 2042 Fragments arranged by size: 2042 1439 931 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2149 2788 2994 3671 4819 5064 1606 Size: 543 639 206 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 639 543 245 206 NaeI GCC'GGC Cuts at: 2089 2089 Size: 5578 NarI GG'CG_CC Cuts at: 2783 2783 Size: 5578 NcoI C'CATG_G Cuts at: 513 1065 2004 2172 2478 513 Size: 552 939 168 306 3613 Fragments arranged by size: 3613 939 552 306 168 NdeI CA'TA_TG Cuts at: 387 387 Size: 5578 NgoMIV G'CCGG_C Cuts at: 2087 2087 Size: 5578 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5578 NotI GC'GGCC_GC Cuts at: 3025 3025 Size: 5578 NspI r_CATG'y Cuts at: 1132 1229 2746 2936 4481 1132 Size: 97 1517 190 1545 2229 Fragments arranged by size: 2229 1545 1517 190 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5578 PciI A'CATG_T Cuts at: 4477 4477 Size: 5578 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5578 PmeI GTTT'AAAC Cuts at: 2882 2882 Size: 5578 PmlI CAC'GTG Cuts at: 2755 2755 Size: 5578 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3647 3679 5160 5192 3647 Size: 32 1481 32 4033 Fragments arranged by size: 4033 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2510 2510 Size: 5578 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5578 PsiI TTA'TAA Cuts at: 3148 3148 Size: 5578 PstI C_TGCA'G Cuts at: 830 2502 2930 830 Size: 1672 428 3478 Fragments arranged by size: 3478 1672 428 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5578 PvuII CAG'CTG Cuts at: 2149 2149 Size: 5578 SalI G'TCGA_C Cuts at: 1936 2920 1936 Size: 984 4594 SapI GCTCTTCn'nnn_ Cuts at: 2420 2685 2420 Size: 265 5313 SbfI CC_TGCA'GG Cuts at: 2502 2930 2502 Size: 428 5150 ScaI AGT'ACT Cuts at: 3826 5538 3826 Size: 1712 3866 SexAI A'CCwGG_T Cuts at: 2265 2265 Size: 5578 SfcI C'TryA_G Cuts at: 826 1045 2110 2498 2926 4061 4742 4933 826 Size: 219 1065 388 428 1135 681 191 1471 Fragments arranged by size: 1471 1135 1065 681 428 388 219 191 SfoI GGC'GCC Cuts at: 2784 2784 Size: 5578 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5578 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5578 SmaI CCC'GGG Cuts at: 1872 2905 1872 Size: 1033 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3682 4583 4845 820 Size: 197 926 1739 901 262 1553 Fragments arranged by size: 1739 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3369 493 Size: 2876 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5578 SphI G_CATG'C Cuts at: 1132 2936 1132 Size: 1804 3774 SspI AAT'ATT Cuts at: 5 52 3502 5 Size: 47 3450 2081 Fragments arranged by size: 3450 2081 47 StuI AGG'CCT Cuts at: 1517 2798 1517 Size: 1281 4297 StyI C'CwwG_G Cuts at: 513 1065 2004 2172 2478 2490 3017 513 Size: 552 939 168 306 12 527 3074 Fragments arranged by size: 3074 939 552 527 306 168 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2681 3605 3622 3775 3960 4405 325 Size: 1255 1101 924 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1101 924 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3824 5536 96 Size: 274 80 33 51 1443 1847 1712 138 Fragments arranged by size: 1847 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5538 5570 5538 Size: 32 5546 Tth111I GACn'n_nGTC Cuts at: 1316 2317 1316 Size: 1001 4577 XbaI T'CTAG_A Cuts at: 2914 2914 Size: 5578 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5578 XmaI C'CCGG_G Cuts at: 1870 2903 1870 Size: 1033 4545 XmnI GAAnn'nnTTC Cuts at: 2129 2447 3707 2129 Size: 318 1260 4000 Fragments arranged by size: 4000 1260 318 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3386 276 Size: 53 83 186 936 1852 2468 Fragments arranged by size: 2468 1852 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FspAI NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SrfI SwaI XcmI