Restriction map for
pFN21AB6592
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AvaII AviII AvrII
BalI BbeI BbsI BbvCI BciVI
BclI BcnI BfrI BlnI BlpI
Bpu1102I BpuAI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrFI BsrGI BssHII Bst1107I
BstAPI BstEII BstPI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HpaI
HpaII KasI KpnI Ksp632I KspI
MaeIII MfeI MluI MluNI MroI
MscI MspI MunI NaeI NarI
NciI NdeI NgoMIV NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PmeI PpuMI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SwaI Tsp45I Tth111I
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccII 185 CGCG CG^CG
AflIII 576 ACGTGT A^C[GA][CT]GT
BamHI 785 GGATCC G^GATCC
BanI 761 GGTGCC G^G[CT][GA]CC
BbrPI 575 CACGTG CAC^GTG
BcgI 720 GCAGCGTTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1204 GCCCAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 60 AGATCT A^GATCT
BsaAI 575 CACGTG [CT]AC^GT[GA]
BsaBI 472 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 298 GAGGAG GAGGAG(10/8)
BsgI 611 CTGCAC GTGCAG(16/14)
BspHI 35 TCATGA T^CATGA
BspMI 303 GCAGGT ACCTGC(4/8)
BsrBI 45 GAGCGG CCGCTC(-3/-3)
BsrDI 151 GCAATG GCAATG(2/0)
BstBI 961 TTCGAA TT^CGAA
BstUI 185 CGCG CG^CG
Cfr13I 27 GGCCC G^G[ACGT]CC
DsaI 1196 CCATGG C^C[GA][CT]GG
MamI 472 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 878 CAGCTG C[AC]G^C[GT]G
MvnI 185 CGCG CG^CG
NcoI 1196 CCATGG C^CATGG
NheI 735 GCTAGC G^CTAGC
NspI 681 ACATGC [GA]CATG^[CT]
NspV 961 TTCGAA TT^CGAA
PflMI 372 CCAAGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 284 GAGTC GAGTC(4/5)
PmaCI 575 CACGTG CAC^GTG
PmlI 575 CACGTG CAC^GTG
PshAI 642 GACAATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 992 CTGCAG CTGCA^G
PvuII 878 CAGCTG CAG^CTG
RcaI 35 TCATGA T^CATGA
Sau96I 27 GGCCC G^G[ACGT]CC
SfuI 961 TTCGAA TT^CGAA
SspI 952 AATATT AAT^ATT
StuI 671 AGGCCT AGG^CCT
TfiI 529 GAATC G^A[AT]TC
TspRI 691 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 372 CCAAGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 804 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AlwNI 82 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
190 CAGTTGCTG
AspHI 628 GAGCTC G[AT]GC[AT]^C
974 GAGCTC
BanII 628 GAGCTC G[GA]GC[CT]^C
974 GAGCTC
BmyI 628 GAGCTC G[AGT]GC[ACT]^C
974 GAGCTC
BsaJI 263 CCTTGG C^C[ACGT][ACGT]GG
1196 CCATGG
BsiHKAI 628 GAGCTC G[AT]GC[AT]^C
974 GAGCTC
BsiYI 372 CCAAGTGTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
486 CCAGGCAGAGG
BslI 372 CCAAGTGTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
486 CCAGGCAGAGG
BsmAI 436 GAGAC GTCTC(1/5)
173 GTCTC
Bsp1286I 628 GAGCTC G[AGT]GC[ACT]^C
974 GAGCTC
BsrI 432 ACTGG ACTGG(1/-1)
481 CCAGT
BssKI 87 CCTGG ^CC[ACGT]GG
486 CCAGG
BssSI 1088 CACGAG CACGAG(-5/-1)
175 CTCGTG
BstNI 87 CCTGG CC^[AT]GG
486 CCAGG
BstXI 890 CCAGCATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1059 CCAGATTGATGG
EcoRI 39 GAATTC G^AATTC
386 GAATTC
EcoRII 87 CCTGG ^CC[AT]GG
486 CCAGG
EcoT14I 263 CCTTGG C^C[AT][AT]GG
1196 CCATGG
HaeIII 27 GGCC GG^CC
672 GGCC
HindIII 128 AAGCTT A^AGCTT
137 AAGCTT
HinfI 284 GAGTC G^A[ACGT]TC
529 GAATC
HphI 260 TCACC GGTGA(8/7)
861 TCACC
MseI 676 TTAA T^TAA
749 TTAA
MvaI 87 CCTGG CC^[AT]GG
486 CCAGG
SacI 628 GAGCTC GAGCT^C
974 GAGCTC
ScrFI 87 CCTGG CC^[ACGT]GG
486 CCAGG
SfcI 875 CTACAG C^T[GA][CT]AG
992 CTGCAG
StyI 263 CCTTGG C^C[AT][AT]GG
1196 CCATGG
Tru9I 676 TTAA T^TAA
749 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BbvI BfaI BpmI BstF5I
BstYI Cac8I DdeI DpnII Fnu4HI
FokI ItaI MaeI MaeII MboI
MboII MflI MnlI MslI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
SfaNI TaqI TseI Tsp509I TthHB8I
XhoII