(Circular) (Six-base) MAPSORT of: pFN21AB6592.seq Check: 9641 from: 1 to: 5923 pFN21AB6592 5923 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3733 278 Size: 53 83 186 936 2197 2468 Fragments arranged by size: 2468 2197 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3266 1937 Size: 1329 4594 Acc65I G'GTAC_C Cuts at: 3244 3244 Size: 5923 AclI AA'CG_TT Cuts at: 1563 4050 4423 1563 Size: 2487 373 3063 Fragments arranged by size: 3063 2487 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2510 3986 5370 5638 1632 Size: 878 1476 1384 268 1917 Fragments arranged by size: 1917 1476 1384 878 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5726 AflIII A'CryG_T Cuts at: 1203 2581 4822 5799 1203 Size: 1378 2241 977 1327 Fragments arranged by size: 2241 1378 1327 977 AgeI A'CCGG_T Cuts at: 4754 4754 Size: 5923 AhdI GACnn_n'nnGTC Cuts at: 4652 4652 Size: 5923 AleI CACnn'nnGTG Cuts at: 1166 2661 1166 Size: 1495 4428 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2796 1184 Size: 1612 4311 AlwNI CAG_nnn'CTG Cuts at: 2092 2200 3666 5238 2092 Size: 108 1466 1572 2777 Fragments arranged by size: 2777 1572 1466 108 ApaLI G'TGCA_C Cuts at: 3980 5136 3980 Size: 1156 4767 ApoI r'AATT_y Cuts at: 1493 2044 2391 2603 2843 3232 3458 3610 5719 Size: 551 347 212 240 389 226 152 2109 Cuts at: 5719 1493 Size: 1697 Fragments arranged by size: 2109 1697 551 389 347 240 226 212 152 AseI AT'TA_AT Cuts at: 160 1032 4477 160 Size: 872 3445 1606 Fragments arranged by size: 3445 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 AvaI C'yCGr_G Cuts at: 1870 1943 3248 1870 Size: 73 1305 4545 Fragments arranged by size: 4545 1305 73 BamHI G'GATC_C Cuts at: 2790 3253 3620 2790 Size: 463 367 5093 Fragments arranged by size: 5093 463 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2766 3244 4699 618 Size: 325 217 627 979 478 1455 1842 Fragments arranged by size: 1842 1455 979 627 478 325 217 BanII G_rGCy'C Cuts at: 1391 2637 2983 3242 1391 Size: 1246 346 259 4072 Fragments arranged by size: 4072 1246 346 259 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4952 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2714 2748 4114 4148 1903 Size: 34 777 34 1366 34 3678 Fragments arranged by size: 3678 1366 777 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3815 5025 3815 Size: 1210 4713 BclI T'GATC_A Cuts at: 1548 1548 Size: 5923 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3215 4534 Size: 107 122 71 631 605 337 1206 1319 Cuts at: 4534 136 Size: 1525 Fragments arranged by size: 1525 1319 1206 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2065 5918 2065 Size: 3853 2070 BlpI GC'TnA_GC Cuts at: 3340 3340 Size: 5923 Bme1580I G_kGCm'C Cuts at: 1790 3984 5140 1790 Size: 2194 1156 2573 Fragments arranged by size: 2573 2194 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5923 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3654 4612 442 Size: 416 53 83 399 24 2237 958 1753 Fragments arranged by size: 2237 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2744 1056 Size: 1688 4235 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2073 2073 Size: 5923 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2136 2459 2492 4583 1239 Size: 897 323 33 2091 2579 Fragments arranged by size: 2579 2091 897 323 33 Bpu10I CC'TnA_GC Cuts at: 3003 3219 3003 Size: 216 5707 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4048 4913 5211 4048 Size: 865 298 4760 Fragments arranged by size: 4760 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4586 882 Size: 617 3087 2219 Fragments arranged by size: 3087 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2582 3714 493 Size: 711 1378 1132 2702 Fragments arranged by size: 2702 1378 1132 711 BsaBI GATnn'nnATC Cuts at: 2481 5917 2481 Size: 3436 2487 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3730 4112 4747 275 Size: 53 83 186 936 2197 382 635 1451 Fragments arranged by size: 2197 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4356 4754 5028 5175 5586 4356 Size: 398 274 147 411 4693 Fragments arranged by size: 4693 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5698 Fragments arranged by size: 5698 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2318 1186 Size: 1132 4791 BseYI C'CCAG_C Cuts at: 2034 5126 5622 2034 Size: 3092 496 2335 Fragments arranged by size: 3092 2335 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2601 1163 Size: 573 865 4485 Fragments arranged by size: 4485 865 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3373 4134 5162 1540 Size: 275 186 1372 761 1028 2301 Fragments arranged by size: 2301 1372 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2637 2983 3984 4069 5140 2637 Size: 346 1001 85 1071 3420 Fragments arranged by size: 3420 1071 1001 346 85 BsmI GAATG_Cn' Cuts at: 3434 3527 3434 Size: 93 5830 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2637 2983 3242 3984 4069 5140 1391 Size: 399 847 346 259 742 85 1071 2174 Fragments arranged by size: 2174 1071 847 742 399 346 259 85 BspEI T'CCGG_A Cuts at: 5586 5586 Size: 5923 BspHI T'CATG_A Cuts at: 2040 3812 5542 2040 Size: 1772 1730 2421 Fragments arranged by size: 2421 1772 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2299 3278 844 Size: 1455 979 3489 Fragments arranged by size: 3489 1455 979 BsrBI CCG'CTC Cuts at: 1394 1823 2052 3810 1394 Size: 429 229 1758 3507 Fragments arranged by size: 3507 1758 429 229 BsrDI GCAATG_nn' Cuts at: 65 1248 2163 4418 4592 65 Size: 1183 915 2255 174 1396 Fragments arranged by size: 2255 1396 1183 915 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4567 4754 1700 Size: 110 2757 187 2869 Fragments arranged by size: 2869 2757 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5923 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5923 BssSI C'ACGA_G Cuts at: 2180 3093 3983 4995 2180 Size: 913 890 1012 3108 Fragments arranged by size: 3108 1012 913 890 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5923 BstBI TT'CG_AA Cuts at: 2967 4727 2967 Size: 1760 4163 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5923 BstXI CCAn_nnnn'nTGG Cuts at: 2902 3071 2902 Size: 169 5754 BstYI r'GATC_y Cuts at: 1972 2065 2088 2277 2790 2991 3123 3253 3620 Size: 93 23 189 513 201 132 130 367 Cuts at: 3620 4005 4022 5474 5560 5572 5918 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 513 385 367 346 201 189 132 130 93 86 23 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3201 513 Size: 552 939 1197 3235 Fragments arranged by size: 3235 1197 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5184 Fragments arranged by size: 5184 683 56 BtsI GCAGTG_nn' Cuts at: 3439 4233 4253 3439 Size: 794 20 5109 Fragments arranged by size: 5109 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5923 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5888 DraI TTT'AAA Cuts at: 3227 3227 Size: 5923 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4930 809 Size: 507 3614 1802 Fragments arranged by size: 3614 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3370 4259 5888 Size: 54 1425 50 275 18 1540 889 1629 Cuts at: 5888 8 Size: 43 Fragments arranged by size: 1629 1540 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3370 1537 Size: 275 1558 4090 Fragments arranged by size: 4090 1558 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3853 1353 Size: 2500 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3023 4510 4884 5030 387 Size: 1064 138 1434 1487 374 146 1280 Fragments arranged by size: 1487 1434 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2635 2981 2635 Size: 346 5577 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2136 2459 2492 2510 3986 4583 5370 Size: 393 504 323 33 18 1476 597 787 Cuts at: 5370 5638 1239 Size: 268 1524 Fragments arranged by size: 1524 1476 787 597 504 393 323 268 33 18 EcoRI G'AATT_C Cuts at: 2044 2391 3232 3610 2044 Size: 347 841 378 4357 Fragments arranged by size: 4357 841 378 347 FspI TGC'GCA Cuts at: 4429 4429 Size: 5923 HaeII r_GCGC'y Cuts at: 1678 5070 1678 Size: 3392 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3007 3039 4564 4596 4638 Size: 32 135 32 1435 32 1525 32 42 Cuts at: 4638 4670 1373 Size: 32 2626 Fragments arranged by size: 2626 1525 1435 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3267 3513 669 Size: 988 281 1329 246 3079 Fragments arranged by size: 3079 1329 988 281 246 HindIII A'AGCT_T Cuts at: 748 2133 2142 748 Size: 1385 9 4529 Fragments arranged by size: 4529 1385 9 HpaI GTT'AAC Cuts at: 3513 3513 Size: 5923 KpnI G_GTAC'C Cuts at: 3248 3248 Size: 5923 MfeI C'AATT_G Cuts at: 3522 3522 Size: 5923 MluI A'CGCG_T Cuts at: 5799 5799 Size: 5923 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5037 5221 1305 Size: 3732 184 2007 Fragments arranged by size: 3732 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4101 Fragments arranged by size: 4101 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2661 2858 3184 3881 4240 4399 518 Size: 648 1495 197 326 697 359 159 2042 Fragments arranged by size: 2042 1495 697 648 359 326 197 159 MspA1I CmG'CkG Cuts at: 1606 2885 3339 4016 5164 5409 1606 Size: 1279 454 677 1148 245 2120 Fragments arranged by size: 2120 1279 1148 677 454 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3201 513 Size: 552 939 1197 3235 Fragments arranged by size: 3235 1197 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5923 NheI G'CTAG_C Cuts at: 1052 2740 1052 Size: 1688 4235 NotI GC'GGCC_GC Cuts at: 3370 3370 Size: 5923 NspI r_CATG'y Cuts at: 1132 1229 2690 3281 4826 1132 Size: 97 1461 591 1545 2229 Fragments arranged by size: 2229 1545 1461 591 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5923 PciI A'CATG_T Cuts at: 4822 4822 Size: 5923 PflMI CCAn_nnn'nTGG Cuts at: 1108 2383 1108 Size: 1275 4648 PfoI T'CCnGG_A Cuts at: 2091 2091 Size: 5923 PmeI GTTT'AAAC Cuts at: 3227 3227 Size: 5923 PmlI CAC'GTG Cuts at: 2582 2582 Size: 5923 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3992 4024 5505 5537 3992 Size: 32 1481 32 4378 Fragments arranged by size: 4378 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2651 1613 Size: 1038 4885 PsiI TTA'TAA Cuts at: 2433 2565 3493 2433 Size: 132 928 4863 Fragments arranged by size: 4863 928 132 PstI C_TGCA'G Cuts at: 830 3001 3275 830 Size: 2171 274 3478 Fragments arranged by size: 3478 2171 274 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5923 PvuII CAG'CTG Cuts at: 2885 2885 Size: 5923 SacI G_AGCT'C Cuts at: 2637 2983 2637 Size: 346 5577 SalI G'TCGA_C Cuts at: 1936 3265 1936 Size: 1329 4594 SbfI CC_TGCA'GG Cuts at: 3275 3275 Size: 5923 ScaI AGT'ACT Cuts at: 4171 5883 4171 Size: 1712 4211 SfcI C'TryA_G Cuts at: 826 1045 2880 2997 3271 4406 5087 5278 826 Size: 219 1835 117 274 1135 681 191 1471 Fragments arranged by size: 1835 1471 1135 681 274 219 191 117 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5923 SmaI CCC'GGG Cuts at: 1872 3250 1872 Size: 1378 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4027 4928 5190 820 Size: 197 926 2084 901 262 1553 Fragments arranged by size: 2084 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3714 493 Size: 3221 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5923 SphI G_CATG'C Cuts at: 1132 3281 1132 Size: 2149 3774 SspI AAT'ATT Cuts at: 5 52 2959 3847 5 Size: 47 2907 888 2081 Fragments arranged by size: 2907 2081 888 47 StuI AGG'CCT Cuts at: 1517 2678 1517 Size: 1161 4762 StyI C'CwwG_G Cuts at: 513 1065 2004 2268 3201 3362 513 Size: 552 939 264 933 161 3074 Fragments arranged by size: 3074 939 933 552 264 161 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3950 3967 4120 4305 4750 325 Size: 1255 2370 17 153 185 445 1498 Fragments arranged by size: 2370 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2418 4169 5881 Size: 274 80 33 51 1443 441 1751 1712 Cuts at: 5881 96 Size: 138 Fragments arranged by size: 1751 1712 1443 441 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2206 2238 2750 2782 3123 3155 5883 5915 2206 Size: 32 512 32 341 32 2728 32 2214 Fragments arranged by size: 2728 2214 512 341 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5923 XbaI T'CTAG_A Cuts at: 2809 3259 2809 Size: 450 5473 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5923 XmaI C'CCGG_G Cuts at: 1870 3248 1870 Size: 1378 4545 XmnI GAAnn'nnTTC Cuts at: 4052 4052 Size: 5923 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3731 276 Size: 53 83 186 936 2197 2468 Fragments arranged by size: 2468 2197 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI