Restriction map for
pFN21AB6306
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
AhdI Alw44I AocI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BciVI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DrdI EaeI EagI Eam1105I EarI
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MaeII MfeI
MluI MluNI MroI MscI MseI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tsp509I Tth111I Van91I XbaI
XcmI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AcyI 576 GACGCC G[GA]^CG[CT]C
AlwI 616 GATCC GGATC(4/5)
Aor51HI 215 AGCGCT AGC^GCT
ApaI 835 GGGCCC GGGCC^C
AvaII 332 GGTCC G^G[AT]CC
BanI 731 GGCACC G^G[CT][GA]CC
BcgI 429 CGACAAGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 531 TGATCA T^GATCA
BfaI 223 CTAG C^TAG
BsaBI 610 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 576 GACGCC G[GA]^CG[CT]C
BsaI 800 GAGACC GGTCTC(1/5)
BseRI 476 CTCCTC GAGGAG(10/8)
BspMI 241 ACCTGC ACCTGC(4/8)
BsrDI 266 GCAATG GCAATG(2/0)
BssSI 184 CACGAG CACGAG(-5/-1)
BstYI 615 AGATCC [GA]^GATC[CT]
DraIII 509 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 538 CCATGG C^C[GA][CT]GG
Eco47III 215 AGCGCT AGC^GCT
EcoT14I 538 CCATGG C^C[AT][AT]GG
FbaI 531 TGATCA T^GATCA
Hin1I 576 GACGCC G[GA]^CG[CT]C
MaeI 223 CTAG C^TAG
MaeIII 520 GTGAC ^GT[ACGT]AC
MamI 610 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 615 AGATCC [GA]^GATC[CT]
MslI 653 CACCTAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 538 CCATGG C^CATGG
PaeR7I 150 CTCGAG C^TCGAG
PpuMI 331 GGGTCCC [GA]G^G[AT]CC[CT]
PvuII 666 CAGCTG CAG^CTG
SmaI 605 CCCGGG CCC^GGG
SmlI 150 CTCGAG C^T[CT][GA]AG
StyI 538 CCATGG C^C[AT][AT]GG
Tsp45I 520 GTGAC ^GT[GC]AC
XhoI 150 CTCGAG C^TCGAG
XhoII 615 AGATCC [GA]^GATC[CT]
XmaCI 605 CCCGGG C^CCGGG
XmaI 605 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AvaI 150 CTCGAG C^[CT]CG[GA]G
605 CCCGGG
BanII 408 GGGCTC G[GA]GC[CT]^C
835 GGGCCC
BbsI 169 GAAGAC GAAGAC(2/6)
718 GAAGAC
BpuAI 169 GAAGAC GAAGAC(2/6)
718 GAAGAC
BsaWI 565 ACCGGA [AT]^CCGG[AT]
792 ACCGGA
BsgI 384 GTGCAG GTGCAG(16/14)
660 GTGCAG
BsmAI 316 GAGAC GTCTC(1/5)
800 GAGAC
BsmFI 36 GTCCC GGGAC(10/14)
333 GTCCC
BsoBI 150 CTCGAG C^[CT]CG[GA]G
605 CCCGGG
BsrI 405 ACTGG ACTGG(1/-1)
728 ACTGG
BstAPI 266 GCAATGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
662 GCAGCAGCTGC
DdeI 122 CTGAG C^T[ACGT]AG
656 CTAAG
DpnII 532 GATC ^GATC
616 GATC
DraII 331 GGGTCCC [GA]G^G[ACGT]CC[CT]
834 AGGGCCC
EcoO109I 331 GGGTCCC [GA]G^G[ACGT]CC[CT]
834 AGGGCCC
FauI 488 CCCGC CCCGC(4/6)
755 CCCGC
HaeII 215 AGCGCT [GA]GCGC^[CT]
674 AGCGCC
HphI 239 TCACC GGTGA(8/7)
599 TCACC
MboI 532 GATC ^GATC
616 GATC
NdeII 532 GATC ^GATC
616 GATC
PstI 279 CTGCAG CTGCA^G
492 CTGCAG
Sau3AI 532 GATC ^GATC
616 GATC
SfaNI 569 GATGC GCATC(5/9)
543 GCATC
TaqI 151 TCGA T^CGA
546 TCGA
TthHB8I 151 TCGA T^CGA
546 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
BbvI BcnI BmyI BsaJI BsiHKAI
BsiYI BslI Bsp1286I BssKI BstF5I
BstNI Cac8I CfoI Cfr13I EcoRII
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII ItaI
MboII MnlI MspA1I MspI MvaI
MwoI NciI NlaIII NlaIV PleI
RsaI Sau96I ScrFI SfcI TseI
TspRI