(Circular) (Six-base) MAPSORT of: pFN21AB6306.seq Check: 9256 from: 1 to: 5545 pFN21AB6306 5545 bp With 182 enzymes: SgfI * October 20, 2009 10:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2255 2255 Size: 5545 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3355 278 Size: 53 83 186 936 1819 2468 Fragments arranged by size: 2468 1819 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2888 1937 Size: 951 4594 Acc65I G'GTAC_C Cuts at: 2866 2866 Size: 5545 AclI AA'CG_TT Cuts at: 1563 3672 4045 1563 Size: 2109 373 3063 Fragments arranged by size: 3063 2109 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3608 4992 5260 1632 Size: 1976 1384 268 1917 Fragments arranged by size: 1976 1917 1384 268 AfeI AGC'GCT Cuts at: 2222 2222 Size: 5545 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5348 AflIII A'CryG_T Cuts at: 1203 4444 5421 1203 Size: 3241 977 1327 Fragments arranged by size: 3241 1327 977 AgeI A'CCGG_T Cuts at: 4376 4376 Size: 5545 AhdI GACnn_n'nnGTC Cuts at: 4274 4274 Size: 5545 AleI CACnn'nnGTG Cuts at: 1166 2662 1166 Size: 1496 4049 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2680 1184 Size: 1496 4049 AlwNI CAG_nnn'CTG Cuts at: 2397 2410 2673 3288 4860 2397 Size: 13 263 615 1572 3082 Fragments arranged by size: 3082 1572 615 263 13 ApaI G_GGCC'C Cuts at: 2844 2844 Size: 5545 ApaLI G'TGCA_C Cuts at: 3602 4758 3602 Size: 1156 4389 ApoI r'AATT_y Cuts at: 1493 2854 3080 3232 5341 1493 Size: 1361 226 152 2109 1697 Fragments arranged by size: 2109 1697 1361 226 152 AseI AT'TA_AT Cuts at: 160 1032 4099 160 Size: 872 3067 1606 Fragments arranged by size: 3067 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5545 AvaI C'yCGr_G Cuts at: 1870 1943 2155 2610 2870 1870 Size: 73 212 455 260 4545 Fragments arranged by size: 4545 455 260 212 73 BamHI G'GATC_C Cuts at: 2875 3242 2875 Size: 367 5178 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2736 2866 4321 618 Size: 325 217 627 949 130 1455 1842 Fragments arranged by size: 1842 1455 949 627 325 217 130 BanII G_rGCy'C Cuts at: 1391 2417 2844 2864 1391 Size: 1026 427 20 4072 Fragments arranged by size: 4072 1026 427 20 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2181 2730 928 Size: 971 282 549 3743 Fragments arranged by size: 3743 971 549 282 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2423 2457 3736 3770 1903 Size: 34 486 34 1279 34 3678 Fragments arranged by size: 3678 1279 486 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3437 4647 3437 Size: 1210 4335 BclI T'GATC_A Cuts at: 1548 2536 1548 Size: 988 4557 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4156 136 Size: 107 122 71 631 605 2484 1525 Fragments arranged by size: 2484 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5540 5540 Size: 5545 BlpI GC'TnA_GC Cuts at: 2962 2962 Size: 5545 Bme1580I G_kGCm'C Cuts at: 1790 2094 2433 2460 2844 3606 4762 1790 Size: 304 339 27 384 762 1156 2573 Fragments arranged by size: 2573 1156 762 384 339 304 27 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5545 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2420 3276 4234 Size: 416 53 83 399 24 1003 856 958 Cuts at: 4234 442 Size: 1753 Fragments arranged by size: 1753 1003 958 856 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5545 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4205 1239 Size: 2966 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3670 4535 4833 3670 Size: 865 298 4382 Fragments arranged by size: 4382 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2799 4208 882 Size: 617 1300 1409 2219 Fragments arranged by size: 2219 1409 1300 617 BsaAI yAC'GTr Cuts at: 493 1204 3336 493 Size: 711 2132 2702 Fragments arranged by size: 2702 2132 711 BsaBI GATnn'nnATC Cuts at: 2619 5539 2619 Size: 2920 2625 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2582 3352 3734 4369 Size: 53 83 186 936 1049 770 382 635 Cuts at: 4369 275 Size: 1451 Fragments arranged by size: 1451 1049 936 770 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2570 2797 3978 4376 4650 4797 5208 2570 Size: 227 1181 398 274 147 411 2907 Fragments arranged by size: 2907 1181 411 398 274 227 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2735 2765 2796 2826 1178 Size: 30 165 30 1332 30 31 30 3897 Fragments arranged by size: 3897 1332 165 31 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2472 1186 Size: 1286 4259 BseYI C'CCAG_C Cuts at: 4748 5244 4748 Size: 496 5049 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2410 2686 1163 Size: 573 674 276 4022 Fragments arranged by size: 4022 674 573 276 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2995 3756 4784 1540 Size: 275 186 994 761 1028 2301 Fragments arranged by size: 2301 1028 994 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2309 2379 2446 2817 3606 3691 4762 2309 Size: 70 67 371 789 85 1071 3092 Fragments arranged by size: 3092 1071 789 371 85 70 67 BsmI GAATG_Cn' Cuts at: 3056 3149 3056 Size: 93 5452 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2094 2309 2379 2417 2433 2446 2460 Size: 399 304 215 70 38 16 13 14 Cuts at: 2460 2817 2844 2864 3606 3691 4762 1391 Size: 357 27 20 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 357 304 215 85 70 38 27 20 16 14 13 BspEI T'CCGG_A Cuts at: 5208 5208 Size: 5545 BspHI T'CATG_A Cuts at: 3434 5164 3434 Size: 1730 3815 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2255 2900 844 Size: 1411 645 3489 Fragments arranged by size: 3489 1411 645 BsrBI CCG'CTC Cuts at: 1394 1823 3432 1394 Size: 429 1609 3507 Fragments arranged by size: 3507 1609 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2278 4040 4214 65 Size: 1183 1030 1762 174 1396 Fragments arranged by size: 1762 1396 1183 1030 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4189 4376 1700 Size: 110 2379 187 2869 Fragments arranged by size: 2869 2379 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5545 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5545 BssSI C'ACGA_G Cuts at: 2189 3605 4617 2189 Size: 1416 1012 3117 Fragments arranged by size: 3117 1416 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2277 2673 794 Size: 1483 396 3666 Fragments arranged by size: 3666 1483 396 BstBI TT'CG_AA Cuts at: 4349 4349 Size: 5545 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5545 BstYI r'GATC_y Cuts at: 1972 2620 2875 3242 3627 3644 5096 5182 5194 Size: 648 255 367 385 17 1452 86 12 Cuts at: 5194 5540 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 648 385 367 346 255 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2543 513 Size: 552 1478 3515 Fragments arranged by size: 3515 1478 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4806 Fragments arranged by size: 4806 683 56 BtsI GCAGTG_nn' Cuts at: 2293 2309 2377 2398 3061 3855 3875 2293 Size: 16 68 21 663 794 20 3963 Fragments arranged by size: 3963 794 663 68 21 20 16 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5545 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5510 DraI TTT'AAA Cuts at: 2849 2849 Size: 5545 DraIII CAC_nnn'GTG Cuts at: 2519 2519 Size: 5545 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4552 809 Size: 507 3236 1802 Fragments arranged by size: 3236 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2992 3881 5510 Size: 54 1425 50 275 18 1162 889 1629 Cuts at: 5510 8 Size: 43 Fragments arranged by size: 1629 1425 1162 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2992 1537 Size: 275 1180 4090 Fragments arranged by size: 4090 1180 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3475 1353 Size: 2122 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4132 4506 4652 387 Size: 1064 138 2543 374 146 1280 Fragments arranged by size: 2543 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3608 4205 4992 5260 1239 Size: 393 1976 597 787 268 1524 Fragments arranged by size: 1976 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2337 2840 2337 Size: 503 5042 EcoRI G'AATT_C Cuts at: 2854 3232 2854 Size: 378 5167 FspI TGC'GCA Cuts at: 4051 4051 Size: 5545 HaeII r_GCGC'y Cuts at: 1678 2224 2683 4692 1678 Size: 546 459 2009 2531 Fragments arranged by size: 2531 2009 546 459 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4186 4218 4260 4292 1373 Size: 32 135 32 2614 32 42 32 2626 Fragments arranged by size: 2626 2614 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2889 3135 669 Size: 988 281 951 246 3079 Fragments arranged by size: 3079 988 951 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5545 HpaI GTT'AAC Cuts at: 3135 3135 Size: 5545 KpnI G_GTAC'C Cuts at: 2870 2870 Size: 5545 MfeI C'AATT_G Cuts at: 3144 3144 Size: 5545 MluI A'CGCG_T Cuts at: 5421 5421 Size: 5545 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4659 4843 1305 Size: 3354 184 2007 Fragments arranged by size: 3354 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3723 Fragments arranged by size: 3723 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2662 3503 3862 4021 518 Size: 648 1496 841 359 159 2042 Fragments arranged by size: 2042 1496 841 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2076 2205 2214 2496 2673 2763 2961 3638 Size: 470 129 9 282 177 90 198 677 Cuts at: 3638 4786 5031 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 470 282 245 198 177 129 90 9 NcoI C'CATG_G Cuts at: 513 1065 2543 513 Size: 552 1478 3515 Fragments arranged by size: 3515 1478 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5545 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5545 NotI GC'GGCC_GC Cuts at: 2992 2992 Size: 5545 NspI r_CATG'y Cuts at: 1132 1229 2903 4448 1132 Size: 97 1674 1545 2229 Fragments arranged by size: 2229 1674 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5545 PciI A'CATG_T Cuts at: 4444 4444 Size: 5545 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5545 PfoI T'CCnGG_A Cuts at: 2339 2339 Size: 5545 PmeI GTTT'AAAC Cuts at: 2849 2849 Size: 5545 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3614 3646 5127 5159 3614 Size: 32 1481 32 4000 Fragments arranged by size: 4000 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2337 2337 Size: 5545 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5545 PsiI TTA'TAA Cuts at: 3115 3115 Size: 5545 PspOMI G'GGCC_C Cuts at: 2840 2840 Size: 5545 PspXI vC'TCGA_Gb Cuts at: 2155 2155 Size: 5545 PstI C_TGCA'G Cuts at: 830 2288 2501 2897 830 Size: 1458 213 396 3478 Fragments arranged by size: 3478 1458 396 213 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5545 PvuII CAG'CTG Cuts at: 2673 2673 Size: 5545 SalI G'TCGA_C Cuts at: 1936 2887 1936 Size: 951 4594 SanDI GG'GwC_CC Cuts at: 2337 2337 Size: 5545 SbfI CC_TGCA'GG Cuts at: 2897 2897 Size: 5545 ScaI AGT'ACT Cuts at: 3793 5505 3793 Size: 1712 3833 SfcI C'TryA_G Cuts at: 826 1045 2284 2497 2827 2893 4028 4709 4900 Size: 219 1239 213 330 66 1135 681 191 Cuts at: 4900 826 Size: 1471 Fragments arranged by size: 1471 1239 1135 681 330 219 213 191 66 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5545 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5545 SmaI CCC'GGG Cuts at: 1872 2612 2872 1872 Size: 740 260 4545 Fragments arranged by size: 4545 740 260 SmlI C'TyrA_G Cuts at: 820 1017 1943 2155 3649 4550 4812 820 Size: 197 926 212 1494 901 262 1553 Fragments arranged by size: 1553 1494 926 901 262 212 197 SnaBI TAC'GTA Cuts at: 493 3336 493 Size: 2843 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5545 SphI G_CATG'C Cuts at: 1132 2903 1132 Size: 1771 3774 SspI AAT'ATT Cuts at: 5 52 3469 5 Size: 47 3417 2081 Fragments arranged by size: 3417 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5545 StyI C'CwwG_G Cuts at: 513 1065 2543 2984 513 Size: 552 1478 441 3074 Fragments arranged by size: 3074 1478 552 441 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2742 3572 3589 3742 3927 4372 325 Size: 1255 1162 830 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1162 830 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2058 3791 5503 Size: 274 80 33 51 1443 81 1733 1712 Cuts at: 5503 96 Size: 138 Fragments arranged by size: 1733 1712 1443 274 138 81 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2796 2828 5505 5537 2796 Size: 32 2677 32 2804 Fragments arranged by size: 2804 2677 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5545 XbaI T'CTAG_A Cuts at: 2881 2881 Size: 5545 XhoI C'TCGA_G Cuts at: 1943 2155 1943 Size: 212 5333 XmaI C'CCGG_G Cuts at: 1870 2610 2870 1870 Size: 740 260 4545 Fragments arranged by size: 4545 740 260 XmnI GAAnn'nnTTC Cuts at: 3674 3674 Size: 5545 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3353 276 Size: 53 83 186 936 1819 2468 Fragments arranged by size: 2468 1819 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI