Restriction map for
pFN21AB6306
(Linear) (Six-base) MAPSORT of: KIBB6306.seq Check: 4777 from: 1 to: 840
KIBB6306.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 250 840
Size: 250 590
AfeI AGC'GCT
Cuts at: 0 217 840
Size: 217 623
AleI CACnn'nnGTG
Cuts at: 0 657 840
Size: 657 183
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 675 840
Size: 675 165
AlwNI CAG_nnn'CTG
Cuts at: 0 392 405 668 840
Size: 392 13 263 172
Fragments arranged by size:
392 263 172 13
ApaI G_GGCC'C
Cuts at: 0 839 840
Size: 839 1
AvaI C'yCGr_G
Cuts at: 0 150 605 840
Size: 150 455 235
Fragments arranged by size:
455 235 150
BanI G'GyrC_C
Cuts at: 0 731 840
Size: 731 109
BanII G_rGCy'C
Cuts at: 0 412 839 840
Size: 412 427 1
Fragments arranged by size:
427 412 1
BbsI GAAGACnn'nnnn_
Cuts at: 0 176 725 840
Size: 176 549 115
Fragments arranged by size:
549 176 115
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 418 452 840
Size: 418 34 388
Fragments arranged by size:
418 388 34
BclI T'GATC_A
Cuts at: 0 531 840
Size: 531 309
Bme1580I G_kGCm'C
Cuts at: 0 89 428 455 839 840
Size: 89 339 27 384 1
Fragments arranged by size:
384 339 89 27 1
BmrI ACTGGGnnnn_n'
Cuts at: 0 415 840
Size: 415 425
BsaI GGTCTCn'nnnn_
Cuts at: 0 794 840
Size: 794 46
BsaBI GATnn'nnATC
Cuts at: 0 614 840
Size: 614 226
BsaHI Gr'CG_yC
Cuts at: 0 577 840
Size: 577 263
BsaWI w'CCGG_w
Cuts at: 0 565 792 840
Size: 565 227 48
Fragments arranged by size:
565 227 48
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 730 760 791 821 840
Size: 730 30 31 30 19
Fragments arranged by size:
730 31 30 30 19
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 467 840
Size: 467 373
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 405 681 840
Size: 405 276 159
Fragments arranged by size:
405 276 159
BsiHKAI G_wGCw'C
Cuts at: 0 304 374 441 812 840
Size: 304 70 67 371 28
Fragments arranged by size:
371 304 70 67 28
Bsp1286I G_dGCh'C
Cuts at: 0 89 304 374 412 428 441 455 812
Size: 89 215 70 38 16 13 14 357
Cuts at: 812 839 840
Size: 27 1
Fragments arranged by size:
357 215 89 70 38 27 16 14
13 1
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 250 840
Size: 250 590
BsrDI GCAATG_nn'
Cuts at: 0 273 840
Size: 273 567
BssSI C'ACGA_G
Cuts at: 0 184 840
Size: 184 656
BstAPI GCAn_nnn'nTGC
Cuts at: 0 272 668 840
Size: 272 396 172
Fragments arranged by size:
396 272 172
BstYI r'GATC_y
Cuts at: 0 615 840
Size: 615 225
BtgI C'CryG_G
Cuts at: 0 538 840
Size: 538 302
BtsI GCAGTG_nn'
Cuts at: 0 288 304 372 393 840
Size: 288 16 68 21 447
Fragments arranged by size:
447 288 68 21 16
DraIII CAC_nnn'GTG
Cuts at: 0 514 840
Size: 514 326
EcoO109I rG'GnC_Cy
Cuts at: 0 332 835 840
Size: 332 503 5
Fragments arranged by size:
503 332 5
HaeII r_GCGC'y
Cuts at: 0 219 678 840
Size: 219 459 162
Fragments arranged by size:
459 219 162
MslI CAynn'nnrTG
Cuts at: 0 657 840
Size: 657 183
MspA1I CmG'CkG
Cuts at: 0 71 200 209 491 668 758 840
Size: 71 129 9 282 177 90 82
Fragments arranged by size:
282 177 129 90 82 71 9
NcoI C'CATG_G
Cuts at: 0 538 840
Size: 538 302
PfoI T'CCnGG_A
Cuts at: 0 334 840
Size: 334 506
PpuMI rG'GwC_Cy
Cuts at: 0 332 840
Size: 332 508
PspOMI G'GGCC_C
Cuts at: 0 835 840
Size: 835 5
PspXI vC'TCGA_Gb
Cuts at: 0 150 840
Size: 150 690
PstI C_TGCA'G
Cuts at: 0 283 496 840
Size: 283 213 344
Fragments arranged by size:
344 283 213
PvuII CAG'CTG
Cuts at: 0 668 840
Size: 668 172
SanDI GG'GwC_CC
Cuts at: 0 332 840
Size: 332 508
SfcI C'TryA_G
Cuts at: 0 279 492 822 840
Size: 279 213 330 18
Fragments arranged by size:
330 279 213 18
SmaI CCC'GGG
Cuts at: 0 607 840
Size: 607 233
SmlI C'TyrA_G
Cuts at: 0 150 840
Size: 150 690
StyI C'CwwG_G
Cuts at: 0 538 840
Size: 538 302
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 737 840
Size: 737 103
TatI w'GTAC_w
Cuts at: 0 53 840
Size: 53 787
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 791 823 840
Size: 791 32 17
Fragments arranged by size:
791 32 17
XhoI C'TCGA_G
Cuts at: 0 150 840
Size: 150 690
XmaI C'CCGG_G
Cuts at: 0 605 840
Size: 605 235
Enzymes that do cut:
AarI AfeI AleI AlfI AlwNI ApaI AvaI BanI
BanII BbsI BcgI BclI Bme1580I BmrI BsaI BsaBI
BsaHI BsaWI BsaXI BseRI BsgI BsiHKAI Bsp1286I BspMI
BsrDI BssSI BstAPI BstYI BtgI BtsI DraIII EcoO109I
HaeII MslI MspA1I NcoI PfoI PpuMI PspOMI PspXI
PstI PvuII SanDI SfcI SmaI SmlI StyI TaqII
TatI TstI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BciVI BfrBI BglI BglII
BlpI BmgBI BmtI BplI BpmI Bpu10I BpuEI BsaAI
BseYI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrFI BsrGI BssHII BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PsrI PvuI
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI StuI SwaI Tth111I XbaI XcmI XmnI ZraI