(Circular) (Six-base) MAPSORT of: pFN21AB5747.seq Check: 6532 from: 1 to: 7126 pFN21AB5747 7126 bp With 182 enzymes: SgfI * April 15, 2010 09:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4936 278 Size: 53 83 186 936 3400 2468 Fragments arranged by size: 3400 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2850 4469 1937 Size: 913 1619 4594 Fragments arranged by size: 4594 1619 913 Acc65I G'GTAC_C Cuts at: 3635 4447 3635 Size: 812 6314 AclI AA'CG_TT Cuts at: 1563 5253 5626 1563 Size: 3690 373 3063 Fragments arranged by size: 3690 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2111 2755 3215 3468 4241 5189 6573 6841 Size: 479 644 460 253 773 948 1384 268 Cuts at: 6841 1632 Size: 1917 Fragments arranged by size: 1917 1384 948 773 644 479 460 268 253 AfeI AGC'GCT Cuts at: 2426 2426 Size: 7126 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6929 AflIII A'CryG_T Cuts at: 1203 6025 7002 1203 Size: 4822 977 1327 Fragments arranged by size: 4822 1327 977 AgeI A'CCGG_T Cuts at: 5957 5957 Size: 7126 AhdI GACnn_n'nnGTC Cuts at: 5855 5855 Size: 7126 AleI CACnn'nnGTG Cuts at: 1166 2685 1166 Size: 1519 5607 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7126 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3673 3705 3673 Size: 32 7094 AlwNI CAG_nnn'CTG Cuts at: 4219 4325 4869 6441 4219 Size: 106 544 1572 4904 Fragments arranged by size: 4904 1572 544 106 ApaLI G'TGCA_C Cuts at: 5183 6339 5183 Size: 1156 5970 ApoI r'AATT_y Cuts at: 1493 2019 2084 2459 3763 3902 3995 4195 4263 Size: 526 65 375 1304 139 93 200 68 Cuts at: 4263 4357 4435 4661 4813 6922 1493 Size: 94 78 226 152 2109 1697 Fragments arranged by size: 2109 1697 1304 526 375 226 200 152 139 94 93 78 68 65 AseI AT'TA_AT Cuts at: 160 1032 5680 160 Size: 872 4648 1606 Fragments arranged by size: 4648 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7126 AvaI C'yCGr_G Cuts at: 1870 1943 4451 1870 Size: 73 2508 4545 Fragments arranged by size: 4545 2508 73 BamHI G'GATC_C Cuts at: 4456 4823 4456 Size: 367 6759 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3328 3635 4447 5902 618 Size: 325 217 627 1541 307 812 1455 1842 Fragments arranged by size: 1842 1541 1455 812 627 325 307 217 BanII G_rGCy'C Cuts at: 1391 2628 3260 4445 1391 Size: 1237 632 1185 4072 Fragments arranged by size: 4072 1237 1185 632 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4229 928 Size: 971 2330 3825 Fragments arranged by size: 3825 2330 971 BbvCI CC'TCA_GC Cuts at: 3934 3934 Size: 7126 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5317 5351 1903 Size: 34 3380 34 3678 Fragments arranged by size: 3678 3380 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2488 5018 6228 2488 Size: 2530 1210 3386 Fragments arranged by size: 3386 2530 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7126 BfrBI ATG'CAT Cuts at: 2391 2676 3475 2391 Size: 285 799 6042 Fragments arranged by size: 6042 799 285 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5737 136 Size: 107 122 71 631 605 4065 1525 Fragments arranged by size: 4065 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7121 7121 Size: 7126 BlpI GC'TnA_GC Cuts at: 2436 4543 2436 Size: 2107 5019 Bme1580I G_kGCm'C Cuts at: 1790 5187 6343 1790 Size: 3397 1156 2573 Fragments arranged by size: 3397 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7126 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2440 4857 5815 Size: 416 53 83 399 24 1023 2417 958 Cuts at: 5815 442 Size: 1753 Fragments arranged by size: 2417 1753 1023 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7126 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2365 3167 3918 4327 5786 1239 Size: 1126 802 751 409 1459 2579 Fragments arranged by size: 2579 1459 1126 802 751 409 Bpu10I CC'TnA_GC Cuts at: 3934 3934 Size: 7126 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2642 5251 6116 6414 2642 Size: 2609 865 298 3354 Fragments arranged by size: 3354 2609 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5789 882 Size: 617 4290 2219 Fragments arranged by size: 4290 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3533 4917 493 Size: 711 2329 1384 2702 Fragments arranged by size: 2702 2329 1384 711 BsaBI GATnn'nnATC Cuts at: 7120 7120 Size: 7126 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4933 5315 5950 275 Size: 53 83 186 936 3400 382 635 1451 Fragments arranged by size: 3400 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5559 5957 6231 6378 6789 5559 Size: 398 274 147 411 5896 Fragments arranged by size: 5896 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4077 4107 4240 4270 1178 Size: 30 165 30 2674 30 133 30 4034 Fragments arranged by size: 4034 2674 165 133 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2084 4080 1186 Size: 898 1996 4232 Fragments arranged by size: 4232 1996 898 BseYI C'CCAG_C Cuts at: 6329 6825 6329 Size: 496 6630 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6553 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4576 5337 6365 1540 Size: 275 186 2575 761 1028 2301 Fragments arranged by size: 2575 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2628 3571 5187 5272 6343 2628 Size: 943 1616 85 1071 3411 Fragments arranged by size: 3411 1616 1071 943 85 BsmI GAATG_Cn' Cuts at: 2676 4637 4730 2676 Size: 1961 93 5072 Fragments arranged by size: 5072 1961 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2628 3260 3571 4445 5187 5272 6343 Size: 399 838 632 311 874 742 85 1071 Cuts at: 6343 1391 Size: 2174 Fragments arranged by size: 2174 1071 874 838 742 632 399 311 85 BspEI T'CCGG_A Cuts at: 6789 6789 Size: 7126 BspHI T'CATG_A Cuts at: 5015 6745 5015 Size: 1730 5396 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4481 844 Size: 3637 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5013 1394 Size: 429 3190 3507 Fragments arranged by size: 3507 3190 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5621 5795 65 Size: 1183 4373 174 1396 Fragments arranged by size: 4373 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5770 5957 1700 Size: 110 3960 187 2869 Fragments arranged by size: 3960 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7126 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7126 BssSI C'ACGA_G Cuts at: 5186 6198 5186 Size: 1012 6114 BstAPI GCAn_nnn'nTGC Cuts at: 794 2682 794 Size: 1888 5238 BstBI TT'CG_AA Cuts at: 5930 5930 Size: 7126 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7126 BstXI CCAn_nnnn'nTGG Cuts at: 3391 4416 3391 Size: 1025 6101 BstYI r'GATC_y Cuts at: 1972 4456 4823 5208 5225 6677 6763 6775 7121 Size: 2484 367 385 17 1452 86 12 346 Cuts at: 7121 1972 Size: 1977 Fragments arranged by size: 2484 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 4188 513 Size: 552 939 2184 3451 Fragments arranged by size: 3451 2184 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6387 Fragments arranged by size: 6387 683 56 BtsI GCAGTG_nn' Cuts at: 2680 2692 3503 4642 5436 5456 2680 Size: 12 811 1139 794 20 4350 Fragments arranged by size: 4350 1139 811 794 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7126 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7091 DraI TTT'AAA Cuts at: 4430 4430 Size: 7126 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6133 809 Size: 507 4817 1802 Fragments arranged by size: 4817 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2582 4573 5462 Size: 54 1425 50 275 18 752 1991 889 Cuts at: 5462 7091 8 Size: 1629 43 Fragments arranged by size: 1991 1629 1425 889 752 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4573 1537 Size: 275 2761 4090 Fragments arranged by size: 4090 2761 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3093 3234 3731 5056 1353 Size: 1740 141 497 1325 3423 Fragments arranged by size: 3423 1740 1325 497 141 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5713 6087 6233 387 Size: 1064 138 4124 374 146 1280 Fragments arranged by size: 4124 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2626 2626 Size: 7126 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2111 2365 2755 3167 3215 3468 3918 Size: 393 479 254 390 412 48 253 450 Cuts at: 3918 4241 4327 5189 5786 6573 6841 1239 Size: 323 86 862 597 787 268 1524 Fragments arranged by size: 1524 862 787 597 479 450 412 393 390 323 268 254 253 86 48 EcoNI CCTnn'n_nnAGG Cuts at: 2039 2039 Size: 7126 EcoO109I rG'GnC_Cy Cuts at: 2315 2315 Size: 7126 EcoRI G'AATT_C Cuts at: 3763 3902 4435 4813 3763 Size: 139 533 378 6076 Fragments arranged by size: 6076 533 378 139 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3150 3424 3150 Size: 274 6852 FspI TGC'GCA Cuts at: 5632 5632 Size: 7126 HaeII r_GCGC'y Cuts at: 1678 2428 6273 1678 Size: 750 3845 2531 Fragments arranged by size: 3845 2531 750 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3242 3274 3445 3477 3740 Size: 32 135 32 1670 32 171 32 263 Cuts at: 3740 3772 5767 5799 5841 5873 1373 Size: 32 1995 32 42 32 2626 Fragments arranged by size: 2626 1995 1670 263 171 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4470 4716 669 Size: 988 281 2532 246 3079 Fragments arranged by size: 3079 2532 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7126 HpaI GTT'AAC Cuts at: 4716 4716 Size: 7126 KpnI G_GTAC'C Cuts at: 3639 4451 3639 Size: 812 6314 MfeI C'AATT_G Cuts at: 4725 4725 Size: 7126 MluI A'CGCG_T Cuts at: 7002 7002 Size: 7126 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3794 6240 6424 1305 Size: 2489 2446 184 2007 Fragments arranged by size: 2489 2446 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2584 10 Size: 54 1425 343 752 4552 Fragments arranged by size: 4552 1425 752 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2381 2685 3185 3967 5084 5443 5602 Size: 648 1215 304 500 782 1117 359 159 Cuts at: 5602 518 Size: 2042 Fragments arranged by size: 2042 1215 1117 782 648 500 359 304 159 MspA1I CmG'CkG Cuts at: 1606 2455 3806 3938 4542 5219 6367 6612 1606 Size: 849 1351 132 604 677 1148 245 2120 Fragments arranged by size: 2120 1351 1148 849 677 604 245 132 NcoI C'CATG_G Cuts at: 513 1065 2004 4188 513 Size: 552 939 2184 3451 Fragments arranged by size: 3451 2184 939 552 NdeI CA'TA_TG Cuts at: 387 2672 3511 387 Size: 2285 839 4002 Fragments arranged by size: 4002 2285 839 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7126 NotI GC'GGCC_GC Cuts at: 4573 4573 Size: 7126 NsiI A_TGCA'T Cuts at: 2393 2678 3477 2393 Size: 285 799 6042 Fragments arranged by size: 6042 799 285 NspI r_CATG'y Cuts at: 1132 1229 2391 3479 4484 6029 1132 Size: 97 1162 1088 1005 1545 2229 Fragments arranged by size: 2229 1545 1162 1088 1005 97 PasI CC'CwG_GG Cuts at: 1348 2311 1348 Size: 963 6163 PciI A'CATG_T Cuts at: 6025 6025 Size: 7126 PflMI CCAn_nnn'nTGG Cuts at: 1108 3192 3218 1108 Size: 2084 26 5016 Fragments arranged by size: 5016 2084 26 PfoI T'CCnGG_A Cuts at: 2034 2334 2790 4228 2034 Size: 300 456 1438 4932 Fragments arranged by size: 4932 1438 456 300 PmeI GTTT'AAAC Cuts at: 4430 4430 Size: 7126 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5195 5227 6708 6740 5195 Size: 32 1481 32 5581 Fragments arranged by size: 5581 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2315 2315 Size: 7126 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7126 PsiI TTA'TAA Cuts at: 4696 4696 Size: 7126 PstI C_TGCA'G Cuts at: 830 2028 2687 4321 4478 830 Size: 1198 659 1634 157 3478 Fragments arranged by size: 3478 1634 1198 659 157 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7126 PvuII CAG'CTG Cuts at: 2455 3806 3938 2455 Size: 1351 132 5643 Fragments arranged by size: 5643 1351 132 SacI G_AGCT'C Cuts at: 2628 2628 Size: 7126 SalI G'TCGA_C Cuts at: 1936 4468 1936 Size: 2532 4594 SapI GCTCTTCn'nnn_ Cuts at: 3093 3093 Size: 7126 SbfI CC_TGCA'GG Cuts at: 4478 4478 Size: 7126 ScaI AGT'ACT Cuts at: 5374 7086 5374 Size: 1712 5414 SfcI C'TryA_G Cuts at: 826 1045 2024 2607 2683 4155 4317 4474 5609 Size: 219 979 583 76 1472 162 157 1135 Cuts at: 5609 6290 6481 826 Size: 681 191 1471 Fragments arranged by size: 1472 1471 1135 979 681 583 219 191 162 157 76 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7126 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7126 SmaI CCC'GGG Cuts at: 1872 4453 1872 Size: 2581 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2621 5230 6131 6393 820 Size: 197 926 678 2609 901 262 1553 Fragments arranged by size: 2609 1553 926 901 678 262 197 SnaBI TAC'GTA Cuts at: 493 3533 4917 493 Size: 3040 1384 2702 Fragments arranged by size: 3040 2702 1384 SpeI A'CTAG_T Cuts at: 152 152 Size: 7126 SphI G_CATG'C Cuts at: 1132 2391 4484 1132 Size: 1259 2093 3774 Fragments arranged by size: 3774 2093 1259 SspI AAT'ATT Cuts at: 5 52 3778 4423 5050 5 Size: 47 3726 645 627 2081 Fragments arranged by size: 3726 2081 645 627 47 StuI AGG'CCT Cuts at: 1517 2812 1517 Size: 1295 5831 StyI C'CwwG_G Cuts at: 513 1065 2004 2292 3384 3824 4188 4397 4565 Size: 552 939 288 1092 440 364 209 168 Cuts at: 4565 513 Size: 3074 Fragments arranged by size: 3074 1092 939 552 440 364 288 209 168 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2324 5153 5170 5323 5508 5953 325 Size: 1255 744 2829 17 153 185 445 1498 Fragments arranged by size: 2829 1498 1255 744 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2115 3808 5372 Size: 274 80 33 51 1443 138 1693 1564 Cuts at: 5372 7084 96 Size: 1712 138 Fragments arranged by size: 1712 1693 1564 1443 274 138 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2299 2331 4077 4109 7086 7118 2299 Size: 32 1746 32 2977 32 2307 Fragments arranged by size: 2977 2307 1746 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7126 XbaI T'CTAG_A Cuts at: 4108 4462 4108 Size: 354 6772 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4413 4413 Size: 7126 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7126 XmaI C'CCGG_G Cuts at: 1870 4451 1870 Size: 2581 4545 XmnI GAAnn'nnTTC Cuts at: 4326 5255 4326 Size: 929 6197 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4934 276 Size: 53 83 186 936 3400 2468 Fragments arranged by size: 3400 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI