(Circular) (Six-base) MAPSORT of: pFN21AB8146.seq Check: 3954 from: 1 to: 5824 pFN21AB8146 5824 bp With 182 enzymes: SgfI * October 20, 2009 10:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3634 278 Size: 53 83 186 936 2098 2468 Fragments arranged by size: 2468 2098 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3167 1937 Size: 1230 4594 Acc65I G'GTAC_C Cuts at: 3145 3145 Size: 5824 AclI AA'CG_TT Cuts at: 1563 3951 4324 1563 Size: 2388 373 3063 Fragments arranged by size: 3063 2388 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2390 2462 2708 3887 5271 5539 1632 Size: 758 72 246 1179 1384 268 1917 Fragments arranged by size: 1917 1384 1179 758 268 246 72 AfeI AGC'GCT Cuts at: 2485 2485 Size: 5824 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5627 AflIII A'CryG_T Cuts at: 1203 4723 5700 1203 Size: 3520 977 1327 Fragments arranged by size: 3520 1327 977 AgeI A'CCGG_T Cuts at: 4655 4655 Size: 5824 AhdI GACnn_n'nnGTC Cuts at: 3064 4553 3064 Size: 1489 4335 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5824 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5824 AlwNI CAG_nnn'CTG Cuts at: 2075 2674 3032 3567 5139 2075 Size: 599 358 535 1572 2760 Fragments arranged by size: 2760 1572 599 535 358 ApaLI G'TGCA_C Cuts at: 3881 5037 3881 Size: 1156 4668 ApoI r'AATT_y Cuts at: 1493 3133 3359 3511 5620 1493 Size: 1640 226 152 2109 1697 Fragments arranged by size: 2109 1697 1640 226 152 AseI AT'TA_AT Cuts at: 160 1032 4378 160 Size: 872 3346 1606 Fragments arranged by size: 3346 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5824 AvaI C'yCGr_G Cuts at: 1870 1943 2127 3149 1870 Size: 73 184 1022 4545 Fragments arranged by size: 4545 1022 184 73 BamHI G'GATC_C Cuts at: 2161 3154 3521 2161 Size: 993 367 4464 Fragments arranged by size: 4464 993 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2151 3145 4600 618 Size: 325 217 627 364 994 1455 1842 Fragments arranged by size: 1842 1455 994 627 364 325 217 BanII G_rGCy'C Cuts at: 1391 3143 1391 Size: 1752 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4853 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2312 2346 4015 4049 1903 Size: 34 375 34 1669 34 3678 Fragments arranged by size: 3678 1669 375 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3716 4926 3716 Size: 1210 4614 BclI T'GATC_A Cuts at: 1548 1548 Size: 5824 BfrBI ATG'CAT Cuts at: 2576 2576 Size: 5824 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4435 136 Size: 107 122 71 631 605 2763 1525 Fragments arranged by size: 2763 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5819 5819 Size: 5824 BlpI GC'TnA_GC Cuts at: 2703 3241 2703 Size: 538 5286 Bme1580I G_kGCm'C Cuts at: 1790 2154 3885 5041 1790 Size: 364 1731 1156 2573 Fragments arranged by size: 2573 1731 1156 364 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5824 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2392 2938 3555 Size: 416 53 83 399 24 975 546 617 Cuts at: 3555 4513 442 Size: 958 1753 Fragments arranged by size: 1753 975 958 617 546 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2201 1056 Size: 1145 4679 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3038 3038 Size: 5824 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2213 4484 1239 Size: 974 2271 2579 Fragments arranged by size: 2579 2271 974 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2168 3949 4814 5112 2168 Size: 1781 865 298 2880 Fragments arranged by size: 2880 1781 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4487 882 Size: 617 2988 2219 Fragments arranged by size: 2988 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3615 493 Size: 711 2411 2702 Fragments arranged by size: 2702 2411 711 BsaBI GATnn'nnATC Cuts at: 5818 5818 Size: 5824 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3631 4013 4648 275 Size: 53 83 186 936 2098 382 635 1451 Fragments arranged by size: 2098 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4257 4655 4929 5076 5487 4257 Size: 398 274 147 411 4594 Fragments arranged by size: 4594 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2370 2400 1178 Size: 30 165 30 967 30 4602 Fragments arranged by size: 4602 967 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2089 2738 1186 Size: 903 649 4272 Fragments arranged by size: 4272 903 649 BseYI C'CCAG_C Cuts at: 2068 2769 5027 5523 2068 Size: 701 2258 496 2369 Fragments arranged by size: 2369 2258 701 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2494 1163 Size: 573 758 4493 Fragments arranged by size: 4493 758 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3274 4035 5063 1540 Size: 275 186 1273 761 1028 2301 Fragments arranged by size: 2301 1273 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3885 3970 5041 3885 Size: 85 1071 4668 Fragments arranged by size: 4668 1071 85 BsmI GAATG_Cn' Cuts at: 2578 3335 3428 2578 Size: 757 93 4974 Fragments arranged by size: 4974 757 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2154 3143 3885 3970 5041 1391 Size: 399 364 989 742 85 1071 2174 Fragments arranged by size: 2174 1071 989 742 399 364 85 BspEI T'CCGG_A Cuts at: 5487 5487 Size: 5824 BspHI T'CATG_A Cuts at: 3713 5443 3713 Size: 1730 4094 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2098 3179 844 Size: 1254 1081 3489 Fragments arranged by size: 3489 1254 1081 BsrBI CCG'CTC Cuts at: 1394 1823 3711 1394 Size: 429 1888 3507 Fragments arranged by size: 3507 1888 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2362 4319 4493 65 Size: 1183 1114 1957 174 1396 Fragments arranged by size: 1957 1396 1183 1114 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4468 4655 1700 Size: 110 2658 187 2869 Fragments arranged by size: 2869 2658 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5824 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5824 BssSI C'ACGA_G Cuts at: 3884 4896 3884 Size: 1012 4812 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5824 BstBI TT'CG_AA Cuts at: 4628 4628 Size: 5824 BstEII G'GTnAC_C Cuts at: 1184 2851 1184 Size: 1667 4157 BstXI CCAn_nnnn'nTGG Cuts at: 2382 2644 2382 Size: 262 5562 BstYI r'GATC_y Cuts at: 1972 2161 2914 3154 3521 3906 3923 5375 5461 Size: 189 753 240 367 385 17 1452 86 Cuts at: 5461 5473 5819 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 753 385 367 346 240 189 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2155 2774 2847 3120 513 Size: 552 1090 619 73 273 3217 Fragments arranged by size: 3217 1090 619 552 273 73 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5085 Fragments arranged by size: 5085 683 56 BtsI GCAGTG_nn' Cuts at: 2057 3340 4134 4154 2057 Size: 1283 794 20 3727 Fragments arranged by size: 3727 1283 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5824 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5789 DraI TTT'AAA Cuts at: 3128 3128 Size: 5824 DraIII CAC_nnn'GTG Cuts at: 2997 2997 Size: 5824 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2691 4831 809 Size: 507 1375 2140 1802 Fragments arranged by size: 2140 1802 1375 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2868 3271 4160 Size: 54 1425 50 275 18 1038 403 889 Cuts at: 4160 5789 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1038 889 403 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3271 1537 Size: 275 1459 4090 Fragments arranged by size: 4090 1459 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2481 2684 2715 3754 1353 Size: 1128 203 31 1039 3423 Fragments arranged by size: 3423 1128 1039 203 31 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2104 2434 2999 4411 4785 4931 Size: 1064 138 515 330 565 1412 374 146 Cuts at: 4931 387 Size: 1280 Fragments arranged by size: 1412 1280 1064 565 515 374 330 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2213 2390 2462 2708 3887 4484 5271 Size: 393 581 177 72 246 1179 597 787 Cuts at: 5271 5539 1239 Size: 268 1524 Fragments arranged by size: 1524 1179 787 597 581 393 268 246 177 72 EcoO109I rG'GnC_Cy Cuts at: 3046 3046 Size: 5824 EcoRI G'AATT_C Cuts at: 3133 3511 3133 Size: 378 5446 EcoRV GAT'ATC Cuts at: 2665 2665 Size: 5824 FspI TGC'GCA Cuts at: 4330 4330 Size: 5824 HaeII r_GCGC'y Cuts at: 1678 2141 2487 4971 1678 Size: 463 346 2484 2531 Fragments arranged by size: 2531 2484 463 346 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2205 2237 2649 2681 3050 Size: 32 135 32 633 32 412 32 369 Cuts at: 3050 3082 4465 4497 4539 4571 1373 Size: 32 1383 32 42 32 2626 Fragments arranged by size: 2626 1383 633 412 369 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3168 3414 669 Size: 988 281 1230 246 3079 Fragments arranged by size: 3079 1230 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5824 HpaI GTT'AAC Cuts at: 3414 3414 Size: 5824 KpnI G_GTAC'C Cuts at: 3149 3149 Size: 5824 MfeI C'AATT_G Cuts at: 3423 3423 Size: 5824 MluI A'CGCG_T Cuts at: 5700 5700 Size: 5824 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2293 2604 4938 5122 1305 Size: 988 311 2334 184 2007 Fragments arranged by size: 2334 2007 988 311 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2870 10 Size: 54 1425 343 1038 2964 Fragments arranged by size: 2964 1425 1038 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2267 2642 3782 4141 4300 518 Size: 648 1101 375 1140 359 159 2042 Fragments arranged by size: 2042 1140 1101 648 375 359 159 MspA1I CmG'CkG Cuts at: 1606 2586 3052 3240 3917 5065 5310 1606 Size: 980 466 188 677 1148 245 2120 Fragments arranged by size: 2120 1148 980 677 466 245 188 NcoI C'CATG_G Cuts at: 513 1065 2847 3120 513 Size: 552 1782 273 3217 Fragments arranged by size: 3217 1782 552 273 NdeI CA'TA_TG Cuts at: 387 387 Size: 5824 NheI G'CTAG_C Cuts at: 1052 2197 1052 Size: 1145 4679 NotI GC'GGCC_GC Cuts at: 3271 3271 Size: 5824 NsiI A_TGCA'T Cuts at: 2578 2578 Size: 5824 NspI r_CATG'y Cuts at: 1132 1229 2711 2767 3182 4727 1132 Size: 97 1482 56 415 1545 2229 Fragments arranged by size: 2229 1545 1482 415 97 56 PasI CC'CwG_GG Cuts at: 1348 2494 2623 1348 Size: 1146 129 4549 Fragments arranged by size: 4549 1146 129 PciI A'CATG_T Cuts at: 4723 4723 Size: 5824 PflMI CCAn_nnn'nTGG Cuts at: 1108 2075 1108 Size: 967 4857 PmeI GTTT'AAAC Cuts at: 3128 3128 Size: 5824 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2417 2449 3893 3925 5406 5438 2417 Size: 32 1444 32 1481 32 2803 Fragments arranged by size: 2803 1481 1444 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3046 3046 Size: 5824 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5824 PsiI TTA'TAA Cuts at: 3394 3394 Size: 5824 PstI C_TGCA'G Cuts at: 830 2018 2550 3057 3176 830 Size: 1188 532 507 119 3478 Fragments arranged by size: 3478 1188 532 507 119 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5824 RsrII CG'GwC_CG Cuts at: 2421 2421 Size: 5824 SalI G'TCGA_C Cuts at: 1936 3166 1936 Size: 1230 4594 SbfI CC_TGCA'GG Cuts at: 3176 3176 Size: 5824 ScaI AGT'ACT Cuts at: 4072 5784 4072 Size: 1712 4112 SfcI C'TryA_G Cuts at: 826 1045 2014 2518 2546 3053 3172 4307 4988 Size: 219 969 504 28 507 119 1135 681 Cuts at: 4988 5179 826 Size: 191 1471 Fragments arranged by size: 1471 1135 969 681 507 504 219 191 119 28 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5824 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5824 SmaI CCC'GGG Cuts at: 1872 2129 3151 1872 Size: 257 1022 4545 Fragments arranged by size: 4545 1022 257 SmlI C'TyrA_G Cuts at: 820 1017 1943 2183 3928 4829 5091 820 Size: 197 926 240 1745 901 262 1553 Fragments arranged by size: 1745 1553 926 901 262 240 197 SnaBI TAC'GTA Cuts at: 493 3615 493 Size: 3122 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5824 SphI G_CATG'C Cuts at: 1132 3182 1132 Size: 2050 3774 SspI AAT'ATT Cuts at: 5 52 3748 5 Size: 47 3696 2081 Fragments arranged by size: 3696 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5824 StyI C'CwwG_G Cuts at: 513 1065 2169 2275 2847 3072 3120 3263 513 Size: 552 1104 106 572 225 48 143 3074 Fragments arranged by size: 3074 1104 572 552 225 143 106 48 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2374 2438 2486 3851 3868 4021 4206 Size: 1255 794 64 48 1365 17 153 185 Cuts at: 4206 4651 325 Size: 445 1498 Fragments arranged by size: 1498 1365 1255 794 445 185 153 64 48 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4070 5782 96 Size: 274 80 33 51 1443 2093 1712 138 Fragments arranged by size: 2093 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2311 2343 2370 2402 5784 5816 2311 Size: 32 27 32 3382 32 2319 Fragments arranged by size: 3382 2319 32 32 32 27 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5824 XbaI T'CTAG_A Cuts at: 3160 3160 Size: 5824 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2587 2587 Size: 5824 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5824 XmaI C'CCGG_G Cuts at: 1870 2127 3149 1870 Size: 257 1022 4545 Fragments arranged by size: 4545 1022 257 XmnI GAAnn'nnTTC Cuts at: 2750 3092 3953 2750 Size: 342 861 4621 Fragments arranged by size: 4621 861 342 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3632 276 Size: 53 83 186 936 2098 2468 Fragments arranged by size: 2468 2098 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI RsrII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI PvuII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI